Standard name | |
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Human Ortholog | |
Description | Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 0 | 2 | 2 |
Bud | 0 | 1 | 5 | 5 | 0 | 4 | – | 5 | 8 | 7 | 6 | 10 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 10 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 4 | 2 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 129 | 130 | 151 | 107 | 148 | 143 | – | 217 | 144 | 180 | 150 | 156 | 141 | 193 | 249 | 20 | 18 | 10 | 124 | 107 | 105 | 84 | 136 | 148 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 1 | 0 | 2 | 0 | 2 | 4 | 0 | 4 | 1 | 1 | 0 | 1 | 3 | 0 | 1 | 1 |
Endosome | 0 | 1 | 1 | 2 | 30 | 2 | – | 2 | 0 | 2 | 0 | 0 | 11 | 12 | 7 | 6 | 4 | 4 | 1 | 3 | 2 | 4 | 5 | 6 |
Golgi | 0 | 2 | 0 | 0 | 0 | 0 | – | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 5 | 1 |
Mitochondria | 39 | 11 | 114 | 109 | 76 | 213 | – | 277 | 329 | 324 | 329 | 349 | 7 | 9 | 6 | 6 | 9 | 2 | 4 | 13 | 29 | 10 | 20 | 13 |
Nucleus | 13 | 25 | 5 | 16 | 23 | 39 | – | 46 | 46 | 37 | 38 | 27 | 55 | 35 | 46 | 28 | 18 | 12 | 53 | 74 | 26 | 32 | 22 | 19 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 1 | 0 | – | 2 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 2 | 0 | – | 7 | 2 | 6 | 6 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 7 | 7 | 5 | 7 | 14 |
Vac/Vac Membrane | 48 | 90 | 42 | 13 | 49 | 61 | – | 33 | 44 | 26 | 11 | 22 | 93 | 97 | 73 | 29 | 25 | 11 | 53 | 72 | 69 | 58 | 81 | 65 |
Unique Cell Count | 200 | 236 | 252 | 188 | 276 | 347 | 456 | 426 | 458 | 421 | 458 | 277 | 320 | 361 | 74 | 64 | 35 | 265 | 293 | 265 | 209 | 306 | 301 | |
Labelled Cell Count | 230 | 260 | 318 | 255 | 330 | 462 | 596 | 579 | 586 | 547 | 588 | 310 | 352 | 381 | 94 | 76 | 40 | 265 | 293 | 265 | 209 | 306 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.3 | 3.6 | 3.4 | 4.3 | 3.1 | – | 3.6 | 2.9 | 3.1 | 2.8 | 2.7 | 4.6 | 4.7 | 4.9 | 6.1 | 5.7 | 6.2 | 4.4 | 5.0 | 4.8 |
Std Deviation (1e-4) | 0.5 | 0.9 | 1.4 | 2.0 | 2.7 | 1.2 | – | 3.8 | 1.6 | 1.1 | 1.3 | 0.6 | 1.0 | 1.5 | 1.8 | 1.4 | 1.5 | 1.6 | 1.3 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.25 | -0.19 | – | 0.01 | -0.32 | -0.23 | -0.33 | -0.41 | 0.36 | 0.4 | 0.46 | 0.77 | 0.68 | 0.79 | 0.3 | 0.47 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 4.1 | 3.9 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5842 | -0.2949 | -0.5411 | -0.2456 | -0.669 | -0.5411 | -0.6376 | -0.718 | -0.449 | -0.2913 | -0.3925 | -0.6291 | 0.2428 | 0.5516 | 0.32 | -0.0587 | 0.0872 | 0.3856 |
Actin | 0.0374 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0.001 | 0.017 | 0.0004 | 0.0073 | 0.0467 | 0.0309 | 0.0067 | 0.035 | 0.0001 | 0.0122 | 0.0003 | 0.0005 | 0.0003 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0021 | 0.0006 | 0.0017 | 0.008 | 0.0012 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0044 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.003 | 0.004 | 0.0004 | 0.0062 | 0.0023 | 0.0014 | 0.004 | 0.0041 | 0.0002 | 0.0004 | 0.0011 | 0.0006 | 0.005 |
Bud Periphery | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0027 | 0.0021 | 0.0031 | 0.0028 | 0.0023 | 0.001 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0029 | 0.0041 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0127 | 0.0032 | 0.0216 | 0.0201 | 0.0072 | 0.0035 | 0.0022 | 0.0005 | 0.0011 | 0.0005 | 0.0001 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.2001 | 0.1507 | 0.1562 | 0.1614 | 0.178 | 0.1955 | 0.1314 | 0.1976 | 0.1558 | 0.1601 | 0.137 | 0.188 | 0.1345 | 0.1364 | 0.1278 | 0.1284 | 0.1586 | 0.1947 |
Cytoplasmic Foci | 0.0263 | 0.0066 | 0.0058 | 0.0024 | 0.0047 | 0.0088 | 0.0394 | 0.0161 | 0.0113 | 0.0219 | 0.0316 | 0.0119 | 0.016 | 0.0033 | 0.0066 | 0.0029 | 0.004 | 0.006 |
Eisosomes | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0058 | 0.0023 | 0.0039 | 0.0017 | 0.0027 | 0.0028 | 0.0127 | 0.0055 | 0.0045 | 0.0049 | 0.0076 | 0.0039 | 0.005 | 0.0039 | 0.003 | 0.0015 | 0.0025 | 0.0033 |
Endosome | 0.0173 | 0.002 | 0.0066 | 0.0017 | 0.0036 | 0.0038 | 0.0316 | 0.0061 | 0.0162 | 0.0224 | 0.0452 | 0.0169 | 0.0089 | 0.0016 | 0.0069 | 0.0028 | 0.0036 | 0.0035 |
Golgi | 0.0024 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0088 | 0.0006 | 0.0067 | 0.0096 | 0.0256 | 0.0027 | 0.0029 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0045 | 0.0004 | 0.0013 | 0.0001 | 0.0004 | 0.0009 | 0.0215 | 0.003 | 0.0042 | 0.0075 | 0.0358 | 0.0093 | 0.0052 | 0.0009 | 0.0028 | 0.0003 | 0.0005 | 0.0005 |
Mitochondria | 0.0013 | 0.0009 | 0.0007 | 0.0005 | 0.0006 | 0.0007 | 0.0096 | 0.0076 | 0.0056 | 0.0087 | 0.0619 | 0.0037 | 0.0022 | 0.0005 | 0.0036 | 0.0018 | 0.0016 | 0.001 |
None | 0.4489 | 0.5174 | 0.4935 | 0.5807 | 0.5479 | 0.39 | 0.4118 | 0.4624 | 0.4444 | 0.4174 | 0.305 | 0.3563 | 0.6251 | 0.6306 | 0.5155 | 0.4447 | 0.6868 | 0.3865 |
Nuclear Periphery | 0.0147 | 0.008 | 0.0235 | 0.0084 | 0.0126 | 0.0102 | 0.0384 | 0.022 | 0.0206 | 0.0228 | 0.0204 | 0.0128 | 0.0097 | 0.0097 | 0.0161 | 0.0104 | 0.0182 | 0.0166 |
Nucleolus | 0.0028 | 0.0029 | 0.0028 | 0.0013 | 0.0019 | 0.0061 | 0.0086 | 0.005 | 0.0035 | 0.0027 | 0.0031 | 0.0056 | 0.003 | 0.0021 | 0.0033 | 0.0042 | 0.0044 | 0.0054 |
Nucleus | 0.1923 | 0.2714 | 0.2492 | 0.2118 | 0.2318 | 0.3189 | 0.1701 | 0.223 | 0.2449 | 0.1758 | 0.2146 | 0.303 | 0.1173 | 0.1987 | 0.264 | 0.3847 | 0.0945 | 0.3493 |
Peroxisomes | 0.0038 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0142 | 0.0017 | 0.0029 | 0.0055 | 0.0267 | 0.0039 | 0.0055 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0287 | 0.0293 | 0.0523 | 0.0279 | 0.0131 | 0.0555 | 0.0518 | 0.0378 | 0.0323 | 0.0549 | 0.0329 | 0.0621 | 0.0192 | 0.0093 | 0.0299 | 0.0135 | 0.0209 | 0.0246 |
Vacuole | 0.0038 | 0.0016 | 0.0015 | 0.001 | 0.0018 | 0.0016 | 0.0077 | 0.0037 | 0.0045 | 0.0037 | 0.0059 | 0.003 | 0.0022 | 0.0015 | 0.003 | 0.0017 | 0.0019 | 0.0019 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0006 | 0.0002 | 0.0004 | 0.0002 | 0.0027 | 0.0009 | 0.0021 | 0.0011 | 0.0034 | 0.0009 | 0.0006 | 0.0003 | 0.001 | 0.0005 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.8328 | 2.3757 | 2.8206 | 7.4969 | 4.8238 | 6.9057 | 5.7649 | 5.5121 | 6.0084 | 7.2057 |
Translational Efficiency | 0.6143 | 1.4709 | 1.1516 | 0.4655 | 0.881 | 1.2781 | 0.6498 | 0.6836 | 0.7518 | 0.9443 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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