Standard name
Human Ortholog
Description Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0.05 0 0 0 0.06 0.08 0.06 0.05 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.96 0.9 0.88 0.81 0.7 0.82 0.72 0.73 0.7 0.98 0.99 0.97 0.95 0.92 0.94 0.97 0.96 0.93 0.88 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0 0 0.06 0.06 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.06 0.09 0.23 0.09 0.16 0.16 0.14 0 0 0 0 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 1 4 6 5 5 3 19 0 0 0 0 0 0 0 0 2 1 1 5
Bud 0 0 0 1 6 0 1 1 5 6 1 6 1 0 0 0 0 0 1 3 6 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 9 24 34 11 7 25 21 20 13 40 30 32 12 6 9 9 22 6 0 0 0 0 0 0
Cytoplasm 272 594 563 424 376 438 509 408 424 447 269 355 232 405 466 358 246 294 257 583 557 216 312 363
Endoplasmic Reticulum 2 3 7 7 6 21 56 15 23 40 21 24 1 1 3 19 10 7 1 2 12 2 6 2
Endosome 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 2 2 0 0 2 2 0 1 1
Golgi 0 0 3 0 1 1 3 5 0 3 1 2 0 1 0 2 3 2 0 1 2 3 4 4
Mitochondria 0 4 0 0 6 3 6 3 19 13 16 35 4 3 4 8 2 1 0 3 2 6 9 9
Nucleus 0 0 0 0 2 2 4 8 4 7 3 6 0 2 4 0 2 0 0 3 2 2 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 0 0 1 1 1 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 4
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Vac/Vac Membrane 1 8 12 11 29 30 55 135 46 101 61 71 3 0 5 11 10 28 2 4 3 1 2 3
Unique Cell Count 274 608 587 440 418 497 629 580 515 625 371 506 237 411 478 375 266 313 266 609 600 247 351 408
Labelled Cell Count 284 633 623 455 435 521 659 601 540 662 409 551 253 418 493 409 297 338 266 609 600 247 351 408


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.6 20.3 20.3 18.8 16.2 18.5 18.0 18.1 16.3 17.6 16.3 15.9 22.4 22.0 20.1 19.5 24.4 23.3 20.6 23.1 24.9
Std Deviation (1e-4) 4.3 5.6 6.5 4.6 7.1 4.6 4.7 4.8 5.1 5.7 5.4 4.5 8.7 9.5 4.2 10.8 14.2 11.7 7.3 9.7 8.4
Intensity Change (Log2) – – – -0.11 -0.33 -0.14 -0.17 -0.17 -0.32 -0.21 -0.32 -0.36 0.14 0.11 -0.01 -0.06 0.27 0.2 0.02 0.19 0.29

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 4.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.6 -3.3 -0.6 -2.1 -1.9 -2.7 0.4 1.4 0.4 -0.4 -3.4 -3.2 -2.5 1.4 -2.7
Cytoplasm 0.4 -3.8 -4.8 -8.1 -11.7 -7.4 -11.4 -10.4 -11.6 1.4 2.4 1.4 -0.3 -2.1 -1.3
Endoplasmic Reticulum 0.5 0.3 3.1 6.1 1.8 3.3 4.7 4.0 3.5 0 0 0 3.6 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.7 3.5 5.1 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.5 3.9 3.4 5.1 10.9 5.1 8.4 8.2 7.5 0 0 -1.3 0.9 1.5 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.6864 22.3801 20.0568 18.0059 18.6742 20.7452 16.3353 24.494 20.98 22.3408 24.5846 24.0096 15.4802 18.2545 17.5082 16.5251 16.9292 16.9564
Actin 0.0213 0.0004 0.0093 0.0302 0.0357 0.004 0.0649 0.0028 0.0091 0.0001 0 0.0031 0.023 0.0043 0.0182 0.0009 0.0014 0.0013
Bud 0.0011 0.0002 0.0007 0.0012 0.0012 0.0003 0.0031 0.0003 0.001 0 0 0 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001
Bud Neck 0.0009 0.0003 0.0006 0.0014 0.0005 0.0017 0.0062 0.0007 0.0004 0.0005 0.0002 0.0005 0.0013 0.001 0.0006 0.0086 0.0004 0.0006
Bud Periphery 0.0013 0.0001 0.0007 0.0014 0.0008 0.0002 0.0052 0.0002 0.0023 0 0 0 0.0002 0.0001 0.0002 0.0003 0 0.0001
Bud Site 0.0052 0.0004 0.0012 0.0031 0.0009 0.0001 0.006 0.0466 0.0065 0 0 0 0.0054 0.0117 0.0051 0.0069 0.0001 0
Cell Periphery 0.0008 0.0001 0.0003 0.0005 0.0002 0.0001 0.0009 0.0004 0.0003 0 0 0 0.0001 0.0006 0.0001 0.0006 0 0
Cytoplasm 0.8496 0.9871 0.9101 0.7805 0.8661 0.959 0.6477 0.9327 0.8975 0.9953 0.9958 0.9775 0.9097 0.9772 0.9272 0.9263 0.9818 0.9788
Cytoplasmic Foci 0.0216 0.0005 0.012 0.0149 0.0139 0.0054 0.0468 0.0011 0.0108 0.0012 0.0004 0.001 0.0128 0.0012 0.0082 0.0063 0.007 0.0017
Eisosomes 0.0008 0 0.0002 0.0003 0 0 0.0004 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0218 0.0008 0.0045 0.0088 0.0017 0.0031 0.0165 0.012 0.0182 0.0006 0.0006 0.0056 0.0117 0.0008 0.0017 0.001 0.0016 0.0034
Endosome 0.021 0.0003 0.0224 0.0362 0.0318 0.0033 0.0882 0.0008 0.0195 0.0005 0.0002 0.0062 0.007 0.0005 0.0165 0.0047 0.0039 0.0015
Golgi 0.0094 0.0001 0.012 0.0269 0.0065 0.0033 0.0161 0.0003 0.0045 0 0 0.0016 0.0035 0.0002 0.0057 0.001 0.0004 0.0002
Lipid Particles 0.0054 0 0.0017 0.0148 0.0178 0.0012 0.0075 0 0.0015 0 0 0.0002 0.0016 0.0001 0.0053 0.0043 0 0.0001
Mitochondria 0.0085 0.0066 0.012 0.056 0.0016 0.013 0.0225 0.0001 0.0183 0 0 0.0005 0.0011 0 0.0023 0.0024 0.0002 0.0002
None 0.0096 0.0002 0.0005 0.0011 0.0003 0.0003 0.013 0.0002 0.0015 0.0003 0.0002 0.0003 0.0018 0.0007 0.0009 0.0095 0.0009 0.0004
Nuclear Periphery 0.0065 0.0003 0.0028 0.002 0.001 0.0007 0.0132 0.0007 0.0029 0.0002 0.0005 0.0021 0.0055 0.0003 0.0009 0.0005 0.0004 0.0096
Nucleolus 0.0002 0 0.0001 0.0004 0.0001 0 0.0013 0 0.0001 0 0 0 0.0001 0 0 0.0103 0 0
Nucleus 0.0037 0.0021 0.0016 0.0021 0.0017 0.0016 0.0123 0.0009 0.0015 0.0008 0.0019 0.001 0.0042 0.0007 0.0011 0.0071 0.0012 0.0012
Peroxisomes 0.0066 0 0.0023 0.0097 0.0127 0.0017 0.0033 0.0001 0.0014 0 0 0 0.0081 0 0.0038 0.002 0.0002 0
Punctate Nuclear 0.0008 0.0001 0.0003 0.0005 0.0003 0.0001 0.0081 0.0001 0.0006 0 0 0.0001 0.0009 0.0001 0.0008 0.0038 0.0001 0.0002
Vacuole 0.0032 0.0002 0.0034 0.0058 0.0046 0.0006 0.0128 0.0001 0.0014 0.0002 0.0001 0.0001 0.0012 0.0002 0.0007 0.0028 0.0003 0.0003
Vacuole Periphery 0.0011 0.0001 0.0014 0.002 0.0007 0.0002 0.0041 0 0.0008 0.0001 0 0.0001 0.0004 0 0.0003 0.0003 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.5569 48.5844 41.9206 58.082 46.7038 65.6876 78.2551 70.5511 71.6417 58.0577
Translational Efficiency 2.4857 3.1421 2.0399 2.1377 1.9752 2.0926 2.2439 1.9354 1.8028 1.7067

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1335 539 2148 1590 1972 2149 2020 1326 3307 2688 4168 2916

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1517.82 1875.59 1803.98 2803.60 1543.56 1711.18 1864.02 2562.84 1533.17 1744.15 1833.08 2694.12
Standard Deviation 278.97 397.25 349.24 793.02 253.98 419.19 439.11 696.38 264.66 420.07 396.49 760.13
Intensity Change Log 2 — 0.305344 0.249183 0.885280 — 0.148730 0.272156 0.731482 — 0.228503 0.260812 0.809783

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000234 0.000209 0.000136 0.000279 0.000118 0.000099 0.000110 0.000179 0.000165 0.000121 0.000123 0.000234
Bud Neck 0.000293 0.000455 0.000496 0.001349 0.000346 0.000561 0.001438 0.000996 0.000325 0.000540 0.000952 0.001189
Bud Site 0.009340 0.006062 0.006747 0.009695 0.006352 0.006832 0.006538 0.007123 0.007558 0.006678 0.006646 0.008526
Cell Periphery 0.000346 0.000418 0.000645 0.001423 0.000187 0.000084 0.000126 0.000732 0.000251 0.000151 0.000393 0.001109
Cytoplasm 0.915626 0.937350 0.940098 0.900412 0.900176 0.864907 0.778652 0.908233 0.906413 0.879433 0.861854 0.903968
Cytoplasmic Foci 0.037743 0.018605 0.009347 0.006953 0.039262 0.033372 0.012207 0.008240 0.038649 0.030411 0.010733 0.007538
Eisosomes 0.000002 0.000001 0.000001 0.000003 0.000002 0.000002 0.000002 0.000001 0.000002 0.000002 0.000001 0.000002
Endoplasmic Reticulum 0.002102 0.000337 0.000255 0.000077 0.001833 0.000836 0.000891 0.000045 0.001942 0.000736 0.000563 0.000062
Endosome 0.001945 0.001217 0.000830 0.001309 0.003024 0.003617 0.003675 0.000458 0.002588 0.003136 0.002209 0.000922
Golgi 0.010243 0.007492 0.004355 0.004174 0.005772 0.004344 0.001741 0.001969 0.007577 0.004975 0.003088 0.003171
Lipid Particles 0.002115 0.002435 0.003526 0.003685 0.001633 0.000655 0.001433 0.003396 0.001827 0.001012 0.002512 0.003554
Mitochondria 0.000130 0.000106 0.000044 0.000127 0.000371 0.000625 0.000110 0.000103 0.000274 0.000521 0.000076 0.000116
Mitotic Spindle 0.001008 0.002769 0.000781 0.003377 0.000919 0.001332 0.002936 0.001494 0.000955 0.001620 0.001826 0.002521
None 0.000987 0.002104 0.000531 0.002635 0.001101 0.001954 0.001546 0.002617 0.001055 0.001984 0.001023 0.002627
Nuclear Periphery 0.000023 0.000024 0.000028 0.000085 0.000030 0.000069 0.000162 0.000066 0.000027 0.000060 0.000093 0.000076
Nuclear Periphery Foci 0.002531 0.003259 0.001606 0.001823 0.001548 0.000975 0.002365 0.002430 0.001945 0.001433 0.001974 0.002099
Nucleolus 0.000028 0.000038 0.000044 0.000112 0.000055 0.000102 0.000214 0.000070 0.000044 0.000089 0.000126 0.000093
Nucleus 0.003403 0.009319 0.006781 0.031274 0.006149 0.015009 0.032041 0.037425 0.005041 0.013868 0.019023 0.034071
Peroxisomes 0.000120 0.000078 0.000080 0.000089 0.000113 0.000178 0.000168 0.000090 0.000115 0.000158 0.000123 0.000090
Vacuole 0.008470 0.006346 0.021609 0.029980 0.025683 0.060835 0.148989 0.023835 0.018734 0.049908 0.083343 0.027186
Vacuole Periphery 0.003311 0.001374 0.002062 0.001139 0.005326 0.003613 0.004658 0.000497 0.004513 0.003164 0.003320 0.000847

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.48 3.25 -1.29 -1.52 -3.00 1.39 -0.74 -3.21 -3.73 -2.76 2.75 1.80 -3.15 -4.42 -3.98
Bud Neck -2.49 -5.94 -8.68 -7.31 -7.12 -4.69 -15.05 -12.58 -9.67 1.27 -5.59 -15.75 -13.23 -10.58 -4.92
Bud Site 2.67 2.67 -0.01 -2.43 -2.27 -0.58 -1.02 -1.06 -0.48 -0.27 1.05 0.80 -1.20 -1.95 -1.90
Cell Periphery -1.39 -3.77 -5.00 -4.45 -3.25 4.66 2.07 -6.07 -7.41 -6.75 4.36 -3.61 -7.01 -7.91 -5.40
Cytoplasm -2.98 -4.43 10.31 11.26 15.70 6.98 23.86 8.28 1.16 -14.89 6.75 14.78 12.70 4.30 -1.76
Cytoplasmic Foci 6.68 13.49 13.84 5.16 1.13 2.68 15.30 15.60 12.62 1.05 4.48 20.68 21.23 14.56 1.68
Eisosomes 1.97 4.46 -4.27 -5.06 -6.36 -1.34 -4.49 -3.62 -2.40 -0.35 -0.23 -0.15 -5.58 -5.10 -5.60
Endoplasmic Reticulum 2.95 3.14 3.47 2.37 4.86 3.57 3.59 7.08 6.48 4.07 4.06 4.94 6.71 6.77 5.12
Endosome 2.24 4.86 3.11 0.81 -0.19 -1.29 -1.17 10.39 8.75 11.14 -1.58 2.20 8.79 7.56 7.43
Golgi 2.10 8.02 7.99 3.41 1.80 2.45 12.14 10.26 4.81 0.41 4.67 11.86 10.54 4.29 1.67
Lipid Particles -0.75 -4.17 -6.32 -4.33 -2.58 6.29 -1.00 -5.40 -8.03 -5.08 5.19 -4.53 -8.62 -11.46 -5.22
Mitochondria 0.34 2.18 0.52 0.00 -3.32 -0.75 1.05 1.17 2.48 0.78 -1.06 1.33 1.20 2.44 -0.80
Mitotic Spindle -1.68 0.33 -2.88 -0.48 -2.99 -1.79 -4.27 -2.36 -1.00 2.68 -2.42 -3.97 -3.64 -1.77 -0.14
None -2.52 2.22 -4.90 -0.76 -8.53 -1.55 -0.92 -3.99 -1.34 -3.56 -2.23 0.24 -5.68 -1.39 -7.98
Nuclear Periphery -0.29 -0.97 -6.62 -7.93 -6.71 -4.17 -9.21 -8.85 -3.59 3.76 -4.05 -8.31 -10.61 -5.52 -0.87
Nuclear Periphery Foci -1.44 2.03 0.19 1.89 -3.21 3.17 -3.85 -6.48 -8.93 -1.56 2.32 -1.06 -3.82 -6.89 -3.05
Nucleolus -0.87 -3.92 -6.17 -4.51 -5.04 -7.05 -17.01 -6.30 2.21 13.36 -7.81 -16.06 -7.19 -1.61 2.96
Nucleus -3.28 -7.85 -22.04 -13.32 -19.02 -9.75 -19.35 -23.10 -17.75 -5.31 -11.44 -19.40 -31.20 -22.14 -15.68
Peroxisomes 1.75 2.23 1.93 -0.35 -0.30 -3.65 -1.85 1.61 3.82 2.55 -2.78 -0.49 2.61 4.48 2.34
Vacuole 1.45 -10.20 -17.16 -17.35 -11.18 -11.02 -28.42 -8.59 2.21 19.95 -12.44 -26.46 -16.64 -1.06 10.91
Vacuole Periphery 2.81 1.83 3.88 1.52 3.05 2.38 2.23 8.30 7.10 8.76 2.47 3.28 8.74 6.66 8.04
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Fes1

Fes1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fes1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available