Standard name
Human Ortholog
Description WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0.07 0 0 0.08 0 0 0 0 0 0.17 0.17 0.19 0.15 0.19 0.13 0 0 0 0 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.27 0.35 0.33 0.2 0.64 0.7 0.48 0.6 0.69 0.68 0.74 0 0.08 0 0.14 0.06 0.07 0 0 0 0 0 0
Nucleus 0.71 0.65 0.76 0.75 0.8 0.61 0.52 0.66 0.6 0.57 0.57 0.52 0.77 0.71 0.72 0.72 0.73 0.77 0.52 0.65 0.56 0.56 0.43 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.13 0.08 0.08 0.05 0.09 0.13 0.17 0.26 0.19 0.24 0.24 0.09 0.08 0.18 0.05 0 0.05 0.2 0.14 0.21 0.26 0.21 0.23
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.06 0.06 0.14
Vac/Vac Membrane 0 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.06 0.09 0.07 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 2 0 0 4 0 2 1 5 0 0 0 0 0 0 1 1 3 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 1 1 0 0 1 5
Bud Site 1 0 0 0 1 4 7 12 14 17 5 8 0 0 0 3 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 2 4 9 7 10 21 14 9 12 4 5 18 16 18 43 36 20 1 1 0 0 5 0
Endoplasmic Reticulum 0 1 0 0 1 0 0 1 0 0 0 0 1 1 0 5 4 2 0 0 0 0 0 2
Endosome 3 1 2 0 4 0 0 1 0 0 0 0 1 4 2 15 3 1 9 3 5 0 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3 2 2 0 1 0 0 0
Mitochondria 73 25 62 43 37 163 189 204 205 238 100 101 4 7 0 40 11 10 1 3 3 0 4 1
Nucleus 129 61 134 97 149 157 140 281 202 197 84 71 80 66 68 201 138 114 99 82 112 30 49 33
Nuclear Periphery 0 0 0 2 1 3 3 3 6 5 0 6 0 0 0 3 4 1 0 0 2 0 0 1
Nucleolus 36 12 14 10 9 24 35 71 89 64 36 33 9 7 17 13 7 8 37 17 42 14 23 24
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 9 3 3 2 6 8 3 0 1 0 1 1 1 2 5 2 12 5 10 3 7 14
Vac/Vac Membrane 4 13 2 3 4 4 1 2 1 3 0 0 3 2 3 6 11 7 14 7 19 3 9 11
Unique Cell Count 182 94 177 130 187 256 271 426 339 345 148 137 104 93 94 278 189 149 190 127 202 54 113 106
Labelled Cell Count 260 115 229 167 216 371 402 599 532 542 231 224 117 104 109 335 223 168 190 127 202 54 113 106


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.8 4.6 4.5 4.6 3.8 3.4 4.2 3.9 3.7 3.8 3.7 6.0 5.5 5.4 6.8 8.1 8.2 4.5 5.8 6.4
Std Deviation (1e-4) 1.5 0.8 1.1 1.4 1.0 1.0 1.0 1.5 1.1 1.5 1.2 0.9 2.1 2.1 1.6 2.2 2.5 2.7 0.7 2.7 2.7
Intensity Change (Log2) – – – -0.02 0.02 -0.27 -0.44 -0.12 -0.23 -0.3 -0.28 -0.31 0.39 0.27 0.24 0.58 0.82 0.84 -0.02 0.35 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.4 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.461 0.0035 0.0331 0 0.0002 0.0015 – – – – – – 0.0006 0 0.0049 0 0.0044 0
Bud 0.0032 0.0004 0.0002 0 0.0001 0.0001 – – – – – – 0.0001 0.0001 0.0008 0 0.0002 0
Bud Neck 0.0045 0.0007 0.0008 0.0003 0.0016 0.0032 – – – – – – 0.0015 0.0003 0.0002 0.0001 0.0013 0.0016
Bud Periphery 0.0057 0.001 0.0002 0 0.0001 0.0003 – – – – – – 0.0001 0.0001 0.0009 0 0.0005 0
Bud Site 0.0211 0.0008 0.0015 0.0001 0.0002 0.0004 – – – – – – 0.0004 0.0004 0.0013 0.0001 0.0005 0.0001
Cell Periphery 0.0009 0.0003 0.0001 0 0 0.0001 – – – – – – 0 0 0.0001 0 0.0003 0
Cytoplasm 0.0022 0.012 0.0011 0.0001 0.0016 0.0005 – – – – – – 0.0029 0.0044 0.0038 0 0.0006 0.0002
Cytoplasmic Foci 0.0155 0.0002 0.0139 0 0.0003 0.0001 – – – – – – 0.0061 0.0001 0.0002 0 0.0159 0
Eisosomes 0.0037 0.0001 0.0002 0 0 0 – – – – – – 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0073 0.0007 0.0004 0 0.0002 0.0007 – – – – – – 0.0007 0 0.002 0 0.0004 0
Endosome 0.0256 0.0035 0.0119 0 0.0011 0.0014 – – – – – – 0.0082 0.0001 0.0017 0 0.0142 0
Golgi 0.0282 0.0017 0.0049 0 0.0001 0.0001 – – – – – – 0.0009 0 0.0002 0 0.0041 0
Lipid Particles 0.0243 0.0003 0.0041 0 0.0002 0.0001 – – – – – – 0.0067 0 0.0001 0 0.0038 0
Mitochondria 0.0146 0.0175 0.0011 0.0001 0.0006 0.0009 – – – – – – 0.0001 0.0007 0.004 0.0001 0.0016 0.0001
None 0.0091 0.0006 0.0003 0.0001 0.0035 0.0003 – – – – – – 0.0008 0.0854 0.0923 0 0.0003 0.0001
Nuclear Periphery 0.0069 0.0133 0.0018 0.0007 0.0135 0.0099 – – – – – – 0.114 0.0016 0.0326 0.0001 0.0307 0.0009
Nucleolus 0.0178 0.0707 0.0505 0.0651 0.1353 0.1245 – – – – – – 0.0321 0.0849 0.0417 0.0594 0.2208 0.0619
Nucleus 0.3176 0.8621 0.8587 0.9313 0.766 0.8429 – – – – – – 0.104 0.8139 0.7956 0.9389 0.3587 0.9243
Peroxisomes 0.0225 0.0002 0.003 0 0.0001 0.0001 – – – – – – 0.0005 0 0.0001 0 0.2215 0
Punctate Nuclear 0.0047 0.0047 0.0121 0.002 0.0748 0.0122 – – – – – – 0.7189 0.0077 0.0154 0.0011 0.0036 0.0104
Vacuole 0.0016 0.0021 0.0003 0.0001 0.0004 0.0005 – – – – – – 0.0006 0.0002 0.001 0 0.0737 0.0001
Vacuole Periphery 0.0019 0.0037 0.0001 0 0.0002 0.0003 – – – – – – 0.0006 0.0001 0.001 0 0.043 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.293 19.016 10.9244 11.4667 13.3453 11.6589 12.0944 14.8607 13.657 14.4467
Translational Efficiency 1.5562 0.6545 0.9302 0.7962 0.9857 1.0531 1.2756 0.6632 0.7611 0.8126

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
74 1382 162 1450 1490 1401 276 2099 1564 2783 438 3549

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 725.42 715.21 877.96 856.17 759.55 775.80 910.05 916.73 757.94 745.71 898.18 891.99
Standard Deviation 67.06 115.67 92.57 118.44 84.29 101.79 84.45 126.79 83.87 113.04 88.90 126.99
Intensity Change Log 2 — -0.020450 0.275339 0.239081 — 0.030540 0.260801 0.271352 — 0.005856 0.267921 0.255677

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000347 0.001076 0.001160 0.001241 0.000065 0.001024 0.001392 0.001449 0.000079 0.001050 0.001306 0.001364
Bud Neck 0.001044 0.011189 0.010628 0.026834 0.008056 0.010473 0.002922 0.051462 0.007724 0.010828 0.005772 0.041399
Bud Site 0.000828 0.006430 0.001868 0.009246 0.000687 0.003634 0.001211 0.015748 0.000693 0.005023 0.001454 0.013091
Cell Periphery 0.000234 0.000199 0.000075 0.000176 0.000067 0.000197 0.000067 0.000158 0.000075 0.000198 0.000070 0.000166
Cytoplasm 0.075380 0.063252 0.005976 0.024795 0.045316 0.030161 0.007739 0.041688 0.046739 0.046594 0.007087 0.034786
Cytoplasmic Foci 0.073342 0.016914 0.000711 0.001332 0.003798 0.007540 0.000564 0.004114 0.007089 0.012195 0.000618 0.002977
Eisosomes 0.000128 0.000062 0.000061 0.000039 0.000018 0.000050 0.000071 0.000033 0.000023 0.000056 0.000067 0.000035
Endoplasmic Reticulum 0.001613 0.005258 0.004818 0.007634 0.000826 0.003810 0.005608 0.004409 0.000863 0.004529 0.005316 0.005727
Endosome 0.007222 0.005350 0.000460 0.002798 0.000642 0.003067 0.000445 0.005507 0.000954 0.004200 0.000451 0.004400
Golgi 0.001534 0.004023 0.000211 0.000679 0.000130 0.002713 0.000189 0.002642 0.000197 0.003364 0.000197 0.001840
Lipid Particles 0.012614 0.005201 0.000720 0.000753 0.001341 0.003580 0.000992 0.000533 0.001875 0.004385 0.000891 0.000623
Mitochondria 0.002275 0.015666 0.000803 0.004181 0.003843 0.012892 0.000885 0.007886 0.003769 0.014270 0.000855 0.006372
Mitotic Spindle 0.000455 0.020848 0.001022 0.018372 0.000802 0.031361 0.003579 0.077766 0.000785 0.026141 0.002633 0.053499
None 0.037108 0.037235 0.006859 0.010672 0.012351 0.016666 0.006774 0.010343 0.013523 0.026880 0.006805 0.010477
Nuclear Periphery 0.000544 0.002654 0.000453 0.002679 0.002242 0.002827 0.000438 0.004277 0.002161 0.002741 0.000444 0.003624
Nuclear Periphery Foci 0.006038 0.005131 0.001403 0.001732 0.000511 0.003447 0.001077 0.001806 0.000772 0.004283 0.001198 0.001776
Nucleolus 0.080712 0.098132 0.049896 0.013128 0.059303 0.093531 0.039723 0.021342 0.060316 0.095816 0.043486 0.017986
Nucleus 0.682495* 0.687358* 0.910137* 0.861340* 0.857973* 0.764320* 0.921581* 0.727829* 0.849670* 0.726102* 0.917348* 0.782377*
Peroxisomes 0.010465 0.004507 0.000639 0.000633 0.000508 0.002958 0.001282 0.001384 0.000979 0.003728 0.001044 0.001077
Vacuole 0.005203 0.006622 0.001931 0.009177 0.001170 0.004022 0.003308 0.015090 0.001360 0.005313 0.002799 0.012674
Vacuole Periphery 0.000417 0.002891 0.000170 0.002561 0.000350 0.001725 0.000154 0.004535 0.000353 0.002304 0.000160 0.003729

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.57 -5.67 -10.14 -4.06 0.14 -5.81 -8.69 -9.74 -1.23 2.22 -10.68 -11.22 -16.05 -2.66 2.00
Bud Neck -13.47 -3.45 -19.72 -12.32 -5.40 -2.73 1.53 -23.44 -21.54 -23.21* -3.88 -0.61 -26.08 -24.71 -19.16
Bud Site -6.00 -3.27 -7.67 -1.74 -4.82 -4.99 -3.62 -10.45 -5.10 -7.08 -7.88 -4.90 -12.97 -4.78 -8.55
Cell Periphery 0.87 3.44 2.09 2.58 -3.11 -1.48 -2.32 -6.29 0.92 -1.54 -2.82 -1.62 -7.08 1.55 -3.38
Cytoplasm 1.26 4.95 4.18 10.78 -4.32 3.34 8.14 5.54 1.90 -4.48 -0.02 10.49 7.52 9.16 -5.75
Cytoplasmic Foci 3.65 4.56 4.56 9.58 -0.00 -3.24 5.79 4.65 6.81 -1.34 -2.89 6.69 6.35 11.61 -1.16
Eisosomes 4.11 3.58 4.96 5.00 2.89 -11.42 -9.54 -13.25 4.29 6.71 -13.54 -10.21 -9.84 6.91 7.18
Endoplasmic Reticulum -8.37 -6.78 -16.39 -9.37 -5.50 -11.65 -11.25 -23.16 -5.90 2.76 -17.66 -14.03 -30.13 -10.33 -1.14
Endosome 0.97 2.08 1.82 4.78 -3.36 -4.81 -1.01 -3.59 1.72 -3.12 -6.56 1.30 -2.54 4.60 -4.34
Golgi -2.69 3.20 2.93 5.29 -0.71 -4.10 -3.07 -4.28 2.66 -3.61 -6.83 -1.69 -4.11 5.50 -3.61
Lipid Particles 3.31 4.98 5.03 8.44 0.66 -4.76 0.26 4.37 7.67 3.83 -5.66 2.88 5.70 11.48 3.67
Mitochondria -8.32 3.71 -2.40 7.68 -9.38 -5.75 3.59 -1.70 5.25 -9.70 -8.30 3.73 -1.20 9.03 -12.74
Mitotic Spindle -8.72 -2.63 -8.65 0.53 -7.45 -9.51 -1.76 -15.71 -5.47 -12.20 -12.68 -2.12 -17.57 -5.08 -13.69
None 0.23 2.87 2.80 11.93 -0.58 -2.54 3.90 3.03 5.60 -2.05 -7.20 4.55 4.07 12.95 -1.89
Nuclear Periphery -5.06 -0.65 -12.26 -2.95 -11.98 -1.19 3.12 -3.21 -2.50 -7.05 -1.24 3.54 -4.13 -3.70 -11.13
Nuclear Periphery Foci 1.08 1.60 2.28 4.90 0.63 -5.99 -1.71 -1.30 5.35 1.47 -8.29 -1.88 -1.45 7.56 1.52
Nucleolus -0.24 1.63 4.47 20.84 5.55 -6.74 4.16 9.37 16.85 2.21 -7.71 3.14 11.98 25.99 5.59
Nucleus -1.40 -6.36 -5.80 -17.82 2.36 11.07 -4.79 9.67 -2.68 11.87 15.80 -6.00 4.86 -13.17 10.70
Peroxisomes 1.33 2.24 2.32 8.11 2.02 -7.33 -1.30 0.08 6.91 1.29 -7.80 -0.22 2.07 10.51 1.60
Vacuole 1.00 1.55 -0.05 -4.30 -7.01 -5.00 -3.37 -9.91 -7.04 -1.82 -5.44 -3.19 -11.41 -7.90 -4.00
Vacuole Periphery -4.53 2.81 -1.35 2.40 -1.90 -3.51 0.76 -2.80 0.39 -3.30 -5.57 0.88 -3.00 2.12 -3.72
Standard name
Human Ortholog
Description WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
Localization
Cell Percentages nucleus (89%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sif2

Sif2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sif2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available