Standard name
Human Ortholog
Description Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.1 0.15 0.19 0.11 0.15 0.17 0.11 0.12 0.11 0.09 0.11 0.09 0.05 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.54 0.59 0.5 0.48 0.6 0.46 0.38 0.39 0.26 0.29 0.23 0.2 0.63 0.64 0.62 0.47 0.3 0.21 0.27 0.3 0.27 0.42 0.4 0.42
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.08 0 0 0 0 0 0
Endosome 0.08 0.05 0 0 0 0.08 0 0 0 0 0 0 0 0 0.17 0.3 0.41 0.35 0.14 0.14 0.06 0.08 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.16 0 0 0 0 0.07 0 0.45 0.28 0.37 0.38 0 0 0 0.09 0.1 0.17 0.11 0.09 0.2 0.08 0.08 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.33 0.2 0.36 0.43 0.29 0.43 0.46 0.51 0.37 0.45 0.41 0.45 0.27 0.25 0.26 0.21 0.3 0.37 0.36 0.35 0.4 0.32 0.35 0.35
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.12 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 6 5 3 0 3 2 1 2 0 1 0 3 0 0 0 0 2 3 0 1 9 10
Bud 1 2 1 3 1 6 8 17 20 7 13 11 1 1 4 0 0 2 2 0 0 1 6 2
Bud Neck 3 0 6 18 33 34 45 73 31 37 24 22 25 39 25 0 0 1 1 1 0 0 5 4
Bud Site 1 0 0 0 0 4 0 2 9 11 3 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 0 3 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 1
Cytoplasm 115 55 29 57 102 145 115 167 70 91 50 52 149 289 327 20 36 11 77 41 26 67 206 196
Endoplasmic Reticulum 0 5 1 0 0 0 1 0 0 5 2 3 3 3 1 3 8 4 1 0 0 0 4 0
Endosome 17 5 0 1 0 25 13 7 3 12 6 8 6 20 87 13 49 18 40 19 5 11 37 27
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 1
Mitochondria 14 15 1 1 0 11 21 18 123 86 80 97 0 1 1 4 12 9 32 12 19 13 42 45
Nucleus 0 0 0 0 1 0 2 2 1 1 1 2 0 3 0 4 3 3 0 0 0 0 2 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 1 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 4 5 0 0 0 0 1 1
Peroxisomes 1 0 0 0 0 1 4 2 3 1 2 6 0 4 13 0 2 2 1 0 0 1 1 0
SpindlePole 71 19 21 52 50 136 139 218 101 139 89 116 63 115 138 9 36 19 102 48 39 50 181 164
Vac/Vac Membrane 1 2 0 0 0 3 2 2 1 1 1 0 1 2 0 8 15 8 11 6 0 3 6 6
Unique Cell Count 213 94 58 120 170 314 300 430 273 311 219 257 236 454 526 43 120 52 284 139 98 158 519 472
Labelled Cell Count 227 105 66 137 193 365 353 510 365 394 272 326 248 481 597 61 165 82 284 139 98 158 519 472


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.3 4.4 4.3 4.6 4.0 3.9 4.2 3.4 3.6 3.6 3.6 4.6 4.8 4.5 5.4 5.7 6.2 5.0 5.3 5.4
Std Deviation (1e-4) 0.7 0.7 0.5 0.7 1.0 1.2 1.1 1.1 1.0 0.8 1.8 0.6 0.9 0.9 0.9 2.2 1.8 2.1 1.2 1.6 1.8
Intensity Change (Log2) – – – -0.04 0.07 -0.13 -0.18 -0.06 -0.39 -0.29 -0.3 -0.31 0.05 0.13 0.02 0.29 0.36 0.48 0.18 0.27 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8871 -1.1081 -1.3459 -0.9899 -1.7526 -1.1623 1.8453 2.2678 2.0163 1.9478 2.2741 2.0972 -0.6066 0.0622 -0.1678 0.2931 -0.4519 -0.2144
Actin 0.0391 0.0033 0.001 0.0417 0.0027 0.003 0.0305 0.0011 0.0153 0.0081 0.0399 0.0059 0.0186 0.0002 0.0043 0.0004 0.015 0.0011
Bud 0.0045 0.0029 0 0.0001 0.0005 0.0001 0.0002 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0.0008
Bud Neck 0.0009 0.0004 0.0007 0.0007 0.0002 0.0016 0.0047 0.0005 0.0036 0.0008 0.0007 0.0054 0.0005 0.0003 0.0008 0.0546 0.0007 0.0108
Bud Periphery 0.0017 0.0003 0 0.0002 0.0028 0.0001 0.0003 0.0017 0.0002 0.0002 0.0003 0.0003 0.0003 0 0.0001 0 0.0001 0.0005
Bud Site 0.007 0.0023 0.0002 0.0006 0.0001 0.0002 0.0017 0.0045 0.0067 0.0002 0.0006 0.0006 0.0006 0.0009 0.0013 0.0001 0.0002 0.0006
Cell Periphery 0.0005 0.0002 0 0 0.0001 0 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001
Cytoplasm 0.0437 0.0912 0.0529 0.0777 0.0645 0.074 0.0321 0.0301 0.031 0.0172 0.0123 0.0395 0.0431 0.0834 0.0447 0.0392 0.0499 0.0779
Cytoplasmic Foci 0.2448 0.1513 0.1821 0.2518 0.3024 0.214 0.2661 0.1634 0.1616 0.3186 0.2114 0.2152 0.2829 0.247 0.234 0.1494 0.277 0.1695
Eisosomes 0.0005 0.0004 0.0001 0.0002 0.0002 0.0002 0.0006 0.0002 0.0003 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.002 0.0028 0.0017 0.0023 0.0019 0.0016 0.0021 0.0017 0.001 0.0027 0.0007 0.0015 0.0017 0.0023 0.0011 0.0004 0.0029 0.001
Endosome 0.0238 0.0202 0.0069 0.019 0.0143 0.015 0.0251 0.0179 0.0139 0.0282 0.0202 0.0122 0.0213 0.0162 0.0147 0.0116 0.0302 0.0093
Golgi 0.0053 0.0025 0.0008 0.003 0.0017 0.0029 0.0065 0.0021 0.0047 0.0143 0.0141 0.0058 0.0028 0.0011 0.0012 0.0016 0.008 0.0009
Lipid Particles 0.0464 0.0318 0.0067 0.0414 0.066 0.0316 0.0328 0.018 0.0151 0.031 0.0749 0.0227 0.0265 0.0167 0.0092 0.0252 0.0511 0.0201
Mitochondria 0.0018 0.0004 0.0002 0.0016 0.0019 0.0027 0.0036 0.0006 0.0066 0.0019 0.0537 0.0045 0.0015 0.0003 0.0003 0.0002 0.0007 0.0003
None 0.2786 0.4588 0.3231 0.3697 0.3664 0.3172 0.3888 0.5443 0.472 0.3714 0.303 0.4309 0.354 0.4465 0.4289 0.4628 0.313 0.4084
Nuclear Periphery 0.0115 0.0062 0.0024 0.006 0.0029 0.0048 0.0087 0.0089 0.0039 0.0128 0.0008 0.0023 0.0023 0.0025 0.0032 0.0015 0.0069 0.0034
Nucleolus 0.0014 0.0017 0.0013 0.0006 0.0005 0.0017 0.0023 0.0029 0.0017 0.0017 0.0019 0.0008 0.0011 0.0008 0.0013 0.0005 0.0014 0.0014
Nucleus 0.0111 0.0176 0.0056 0.0047 0.0011 0.0103 0.0149 0.0034 0.0048 0.0036 0.0006 0.0022 0.0029 0.003 0.0039 0.0018 0.012 0.006
Peroxisomes 0.021 0.0114 0.0033 0.0146 0.0085 0.0126 0.0232 0.0073 0.0131 0.038 0.0482 0.0171 0.0063 0.0036 0.0039 0.0126 0.0286 0.0049
Punctate Nuclear 0.2527 0.1928 0.4106 0.1627 0.1604 0.3057 0.1525 0.1873 0.2434 0.1478 0.2152 0.2315 0.2318 0.1737 0.2462 0.2378 0.2007 0.2824
Vacuole 0.0011 0.0013 0.0004 0.0009 0.0006 0.0005 0.0019 0.0025 0.0008 0.0005 0.0006 0.0007 0.0011 0.0011 0.0006 0.0002 0.0011 0.0006
Vacuole Periphery 0.0006 0.0003 0.0001 0.0004 0.0003 0.0002 0.001 0.0007 0.0003 0.0005 0.0004 0.0004 0.0002 0.0002 0.0001 0.0001 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.9708 5.3462 7.9342 9.537 5.8155 4.4829 11.4868 7.7528 13.0929 8.8232
Translational Efficiency 0.2653 0.5234 0.3986 0.5035 0.5205 0.7408 0.3511 0.6538 0.3961 0.3868

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1235 457 2439 1483 1940 1451 2335 1429 3175 1908 4774 2912

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 588.94 711.97 781.45 808.60 656.05 734.08 834.43 774.41 629.95 728.78 807.36 791.82
Standard Deviation 64.37 183.70 89.75 111.88 81.71 82.39 97.10 153.79 82.23 115.47 97.10 135.18
Intensity Change Log 2 — 0.273696 0.408033 0.457306 — 0.162132 0.346985 0.239292 — 0.215983 0.376186 0.346536

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000168 0.000885 0.000332 0.003716 0.000073 0.000207 0.000305 0.000596 0.000110 0.000369 0.000319 0.002185
Bud Neck 0.016349 0.019927 0.014656 0.023847 0.007523 0.016929 0.014577 0.011629 0.010956 0.017647 0.014618 0.017851
Bud Site 0.003119 0.012534 0.022059 0.076548 0.004058 0.003726 0.018307 0.048231 0.003693 0.005836 0.020224 0.062652
Cell Periphery 0.000203 0.000191 0.000119 0.000274 0.000124 0.000106 0.000228 0.000561 0.000155 0.000126 0.000172 0.000415
Cytoplasm 0.253748* 0.240766* 0.359355* 0.323442* 0.416777* 0.289897* 0.380924* 0.468064* 0.353363* 0.278130* 0.369905* 0.394412*
Cytoplasmic Foci 0.450374* 0.348955* 0.015174 0.029572 0.300898* 0.405283* 0.016986 0.053334 0.359040* 0.391791* 0.016060 0.041232
Eisosomes 0.000249 0.000136 0.000032 0.000065 0.000088 0.000135 0.000051 0.000083 0.000151 0.000135 0.000041 0.000074
Endoplasmic Reticulum 0.000650 0.001483 0.002169 0.003362 0.000626 0.000558 0.001771 0.001309 0.000636 0.000780 0.001974 0.002354
Endosome 0.011773 0.032894 0.003412 0.019495 0.011382 0.022608 0.003572 0.008019 0.011534 0.025072 0.003490 0.013863
Golgi 0.009050 0.007874 0.000233 0.011581 0.001685 0.006480 0.000918 0.009545 0.004550 0.006814 0.000568 0.010582
Lipid Particles 0.065867 0.031486 0.001439 0.002472 0.024460 0.035213 0.001928 0.003642 0.040567 0.034320 0.001678 0.003046
Mitochondria 0.024856 0.020412 0.001922 0.016478 0.003709 0.016067 0.002082 0.001686 0.011935 0.017107 0.002000 0.009219
Mitotic Spindle 0.014132 0.079163 0.162589* 0.160571* 0.019990 0.029044 0.055917 0.067920 0.017711 0.041048 0.110415* 0.115105*
None 0.013236 0.009327 0.010743 0.014579 0.024319 0.006381 0.012703 0.017231 0.020008 0.007086 0.011702 0.015880
Nuclear Periphery 0.000752 0.001671 0.003318 0.003196 0.001063 0.001153 0.002511 0.001363 0.000942 0.001277 0.002924 0.002296
Nuclear Periphery Foci 0.002028 0.006065 0.004361 0.005733 0.004143 0.002227 0.001829 0.003450 0.003320 0.003146 0.003123 0.004613
Nucleolus 0.008643 0.016297 0.008875 0.003004 0.017660 0.009478 0.005784 0.005067 0.014153 0.011111 0.007363 0.004017
Nucleus 0.087570 0.090219 0.365125* 0.188904* 0.143132* 0.098626 0.444336* 0.246957* 0.121520 0.096612 0.403868* 0.217393*
Peroxisomes 0.029082 0.043803 0.000823 0.006973 0.008583 0.031436 0.000811 0.002298 0.016556 0.034398 0.000817 0.004679
Vacuole 0.007231 0.034397 0.023037 0.092859 0.009229 0.022971 0.033976 0.044780 0.008452 0.025708 0.028388 0.069265
Vacuole Periphery 0.000920 0.001517 0.000226 0.013330 0.000479 0.001476 0.000484 0.004233 0.000650 0.001486 0.000352 0.008866

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.86 -14.25 -5.31 -2.22 -3.43 -4.83 -19.35 -11.68 -8.87 -1.18 -4.02 -24.24 -7.52 -5.42 -3.60
Bud Neck -1.08 -4.57 -2.55 -0.57 1.76 -6.80 -12.69 -5.32 1.14 5.38 -4.89 -12.80 -6.20 -0.42 4.92
Bud Site -4.13 -9.05 -11.35 -7.16 -6.51 -0.37 -7.40 -9.56 -9.64 -6.03 -3.15 -11.38 -14.66 -13.52 -8.87
Cell Periphery 0.26 3.08 0.37 0.03 -5.97 0.85 -2.63 -5.21 -5.41 -4.02 1.59 0.37 -4.89 -5.91 -5.24
Cytoplasm 0.81 2.64 -3.04 -2.99 -6.41 6.87 9.34 -3.59 -9.92 -12.49 5.09 9.96 -3.09 -7.85 -13.41
Cytoplasmic Foci 7.77 57.48 56.31 26.39 -1.67 -6.91 52.02 38.14 43.52 -13.61 -0.98 74.89 64.64 54.81 -12.33
Eisosomes 6.10 11.99 8.62 2.73 -8.44 -8.38 -2.75 -7.73 -0.57 -5.14 1.99 10.10 3.47 1.81 -9.29
Endoplasmic Reticulum -3.45 -17.28 -19.20 -12.44 -7.47 0.05 -22.34 -15.61 -15.43 8.76 -2.40 -28.98 -24.37 -21.98 -0.57
Endosome -6.86 6.33 2.41 7.70 -2.48 -5.87 10.52 2.43 7.60 -7.37 -9.13 12.21 3.77 11.20 -6.39
Golgi 1.15 11.33 5.52 3.49 -2.30 -4.89 7.96 -1.87 0.87 -3.12 -2.08 13.59 1.47 2.68 -3.87
Lipid Particles 7.39 18.61 18.12 7.70 -6.10 -2.90 17.07 13.96 13.12 -9.07 4.04 24.24 22.47 15.70 -10.88
Mitochondria 1.56 9.93 7.84 3.45 -2.47 -5.59 6.69 8.50 6.86 3.23 -1.79 10.70 8.12 6.80 -1.79
Mitotic Spindle -7.41 -32.81* -17.17 -3.55 12.59 -0.47 -12.36 -9.62 -8.93 0.37 -5.48 -31.92 -18.31 -11.62 9.92
None 2.27 -0.44 1.97 -1.09 3.74 9.37 4.58 4.39 -4.72 0.74 9.10 3.92 5.29 -4.45 2.84
Nuclear Periphery -2.30 -23.20 -16.73 -10.33 -1.90 0.33 -24.81 -11.86 -9.09 11.49 -1.30 -33.86 -19.89 -15.21 4.47
Nuclear Periphery Foci -4.18 -11.35 -6.70 -0.91 3.74 5.50 -2.25 -3.80 -7.34 -1.90 1.56 -9.03 -6.42 -7.28 1.43
Nucleolus -1.91 -16.11 1.90 2.98 18.21 8.83 0.25 7.45 -0.94 7.05 5.96 -8.15 8.70 2.20 17.37
Nucleus -0.70 -31.57 -24.36 -18.61 4.04 4.58 -43.84 -17.72 -20.74 20.12 2.82 -51.42 -29.06 -29.45 16.68
Peroxisomes -2.65 20.59 17.40 10.31 -2.06 -12.74 19.74 9.92 17.65 -7.57 -9.84 27.28 19.44 20.65 -6.01
Vacuole -6.80 -13.05 -28.53 -20.39 -24.41 -8.47 -20.61 -18.81 -14.57 -4.00 -10.97 -24.05 -32.93 -26.90 -21.78
Vacuole Periphery -2.08 5.92 -2.61 -1.98 -3.38 -3.53 1.55 -3.01 -1.42 -3.16 -3.60 5.87 -3.93 -2.47 -4.55
Standard name
Human Ortholog
Description Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Iml3

Iml3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Iml3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available