Standard name | |
---|---|
Human Ortholog | |
Description | Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability; human homolog ALG1 complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 24 | 28 | 13 | 12 | 10 | 30 | – | 7 | 25 | 18 | 14 | 19 | 13 | 6 | 11 | 11 | 1 | 1 | 10 | 10 | 12 | 15 |
Bud | 1 | 4 | 2 | 2 | 1 | 2 | 7 | 9 | – | 11 | 15 | 8 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 1 | 1 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 7 | 25 | 18 | 35 | 29 | 27 | 36 | – | 34 | 29 | 40 | 46 | 46 | 68 | 24 | 22 | 30 | 0 | 0 | 9 | 9 | 17 | 25 |
Cytoplasm | 14 | 50 | 26 | 51 | 94 | 64 | 86 | 124 | – | 138 | 108 | 101 | 68 | 68 | 104 | 62 | 80 | 59 | 4 | 15 | 2 | 5 | 19 | 23 |
Endoplasmic Reticulum | 17 | 160 | 340 | 173 | 195 | 188 | 254 | 346 | – | 216 | 190 | 246 | 305 | 304 | 326 | 1 | 1 | 2 | 78 | 186 | 387 | 320 | 522 | 539 |
Endosome | 1 | 1 | 0 | 6 | 31 | 9 | 8 | 10 | – | 8 | 1 | 6 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 1 | 1 | 1 | 1 | 2 |
Golgi | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 5 |
Mitochondria | 5 | 5 | 25 | 39 | 30 | 77 | 112 | 137 | – | 178 | 205 | 237 | 1 | 1 | 1 | 12 | 18 | 8 | 3 | 4 | 7 | 10 | 22 | 23 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | – | 4 | 2 | 2 | 0 | 0 | 0 | 1 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 7 | 2 | 1 | 4 | 17 | 18 | – | 31 | 19 | 29 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 2 | 3 | 6 | 6 | 69 | 49 | 119 | 150 | – | 93 | 50 | 74 | 11 | 11 | 30 | 9 | 9 | 10 | 4 | 8 | 3 | 7 | 16 | 27 |
Unique Cell Count | 37 | 213 | 388 | 257 | 382 | 340 | 513 | 693 | 554 | 492 | 584 | 394 | 393 | 461 | 104 | 139 | 116 | 98 | 223 | 430 | 376 | 625 | 681 | |
Labelled Cell Count | 43 | 233 | 458 | 326 | 472 | 437 | 644 | 864 | 720 | 645 | 762 | 446 | 450 | 544 | 118 | 154 | 127 | 98 | 223 | 430 | 376 | 625 | 681 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 8.6 | 8.0 | 7.8 | 8.2 | 7.3 | 7.2 | 7.6 | – | 6.9 | 6.8 | 6.7 | 9.5 | 9.5 | 11.6 | 6.7 | 7.7 | 9.5 | 9.5 | 9.6 | 9.8 |
Std Deviation (1e-4) | 0.9 | 1.4 | 1.1 | 1.2 | 1.2 | 1.4 | 1.3 | 1.2 | – | 1.3 | 1.1 | 1.2 | 1.1 | 1.1 | 1.5 | 1.1 | 1.4 | 1.6 | 1.4 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.03 | -0.13 | -0.14 | -0.06 | – | -0.21 | -0.23 | -0.25 | 0.26 | 0.26 | 0.54 | -0.24 | -0.05 | 0.25 | 0.26 | 0.28 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.2 | -1.8 | -1.6 | -3.3 | -1.3 | – | -4.2 | -0.7 | -2.3 | -1.7 | -0.8 | -2.4 | -0.2 | 0.7 | 1.2 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 1.9 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | 1.4 | 1.1 | -0.8 | -0.9 | – | -0.2 | -0.3 | 0.2 | 2.5 | 2.6 | 3.9 | 5.0 | 3.3 | 5.9 |
Cytoplasm | 5.0 | 6.8 | 5.0 | 4.5 | 5.1 | – | 7.3 | 6.3 | 4.8 | 4.5 | 4.6 | 6.4 | 12.5 | 12.8 | 11.1 |
Endoplasmic Reticulum | -6.3 | -11.0 | -9.8 | -12.0 | -12.3 | – | -14.9 | -14.7 | -14.2 | -3.8 | -3.8 | -6.0 | -17.0 | -18.4 | -17.4 |
Endosome | 0 | 5.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.6 | 0.8 | 6.3 | 6.4 | 5.9 | – | 9.4 | 11.8 | 11.7 | -4.8 | -4.8 | -5.2 | 1.7 | 2.4 | 0.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | -0.7 | 1.4 | 0.8 | – | 2.9 | 1.8 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 7.7 | 6.6 | 9.3 | 9.0 | – | 7.5 | 5.2 | 6.2 | 1.2 | 1.2 | 3.6 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4374 | 5.4711 | 5.6031 | 5.1472 | 4.5353 | 5.3338 | 5.3601 | 6.1863 | 5.7858 | 5.6712 | 5.6224 | 5.8881 | 5.9537 | 6.8256 | 6.5064 | 6.1089 | 6.2329 | 6.3435 |
Actin | 0.0041 | 0.0013 | 0.0173 | 0.0004 | 0.0181 | 0.0012 | 0.0314 | 0.0036 | 0.0028 | 0.0083 | 0.0003 | 0.0086 | 0.0021 | 0.0049 | 0.0035 | 0.0069 | 0.0079 | 0.0012 |
Bud | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0016 | 0 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0007 | 0.0005 | 0.0018 | 0.0042 | 0.0058 | 0.0006 | 0.0016 | 0.0007 | 0.0006 | 0.0093 | 0.0007 | 0.0049 | 0.0001 | 0.001 | 0.0005 | 0.0028 | 0.0029 | 0.0049 |
Bud Periphery | 0.0006 | 0.0002 | 0.0019 | 0.0003 | 0.0027 | 0.0001 | 0.0012 | 0.0004 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0005 | 0.0054 | 0.0039 | 0.0006 | 0.0048 | 0 | 0.0027 | 0.0126 | 0.0008 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0028 | 0.0011 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0012 | 0.0044 | 0.0052 | 0.0056 | 0.0033 | 0.001 | 0.0028 | 0.004 | 0.0039 | 0.0051 | 0.0006 | 0.0046 | 0.0012 | 0.0039 | 0.0032 | 0.003 | 0.0016 | 0.0007 |
Cytoplasm | 0.2057 | 0.2503 | 0.1774 | 0.2517 | 0.1764 | 0.1455 | 0.1313 | 0.2799 | 0.2155 | 0.1248 | 0.0939 | 0.1628 | 0.0846 | 0.2032 | 0.1448 | 0.1769 | 0.1902 | 0.1121 |
Cytoplasmic Foci | 0.0091 | 0.0054 | 0.0078 | 0.0013 | 0.0512 | 0.0016 | 0.0128 | 0.014 | 0.0067 | 0.0367 | 0.0022 | 0.0036 | 0.0033 | 0.0033 | 0.0051 | 0.0039 | 0.0061 | 0.0054 |
Eisosomes | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.6853 | 0.6691 | 0.7294 | 0.6967 | 0.5427 | 0.8149 | 0.6762 | 0.5786 | 0.7236 | 0.6979 | 0.868 | 0.7638 | 0.8695 | 0.7424 | 0.7936 | 0.7506 | 0.7568 | 0.8469 |
Endosome | 0.0349 | 0.0123 | 0.0166 | 0.0019 | 0.0466 | 0.0091 | 0.0385 | 0.0405 | 0.0121 | 0.032 | 0.0141 | 0.0099 | 0.007 | 0.0055 | 0.0101 | 0.0228 | 0.0146 | 0.0081 |
Golgi | 0.0011 | 0.0017 | 0.0065 | 0.0002 | 0.0179 | 0.001 | 0.0078 | 0.0036 | 0.0021 | 0.0154 | 0.0004 | 0.0023 | 0.0013 | 0.0028 | 0.0025 | 0.0058 | 0.004 | 0.001 |
Lipid Particles | 0.0056 | 0.0119 | 0.0114 | 0.0018 | 0.0112 | 0.0029 | 0.0185 | 0.0101 | 0.005 | 0.015 | 0.0012 | 0.0088 | 0.0114 | 0.0119 | 0.0189 | 0.008 | 0.0049 | 0.0069 |
Mitochondria | 0.0003 | 0.0002 | 0.0009 | 0.0001 | 0.0248 | 0.0004 | 0.0031 | 0.0006 | 0.0004 | 0.0018 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0003 | 0.0001 |
None | 0.0109 | 0.0093 | 0.0034 | 0.0225 | 0.009 | 0.0033 | 0.0324 | 0.019 | 0.0122 | 0.0134 | 0.0006 | 0.0107 | 0.0044 | 0.0012 | 0.0057 | 0.001 | 0.001 | 0.0011 |
Nuclear Periphery | 0.0223 | 0.0129 | 0.0074 | 0.0043 | 0.0057 | 0.0119 | 0.0187 | 0.0138 | 0.0071 | 0.0068 | 0.0086 | 0.0099 | 0.0103 | 0.0063 | 0.0049 | 0.0099 | 0.0056 | 0.0055 |
Nucleolus | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0004 | 0 | 0.0005 | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0046 | 0.003 | 0.0014 | 0.0027 | 0.0036 | 0.002 | 0.004 | 0.0065 | 0.0011 | 0.002 | 0.0026 | 0.0018 | 0.0013 | 0.0041 | 0.0007 | 0.0013 | 0.0009 | 0.0026 |
Peroxisomes | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0473 | 0 | 0.0023 | 0.0003 | 0.0013 | 0.02 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.003 | 0.0007 | 0.0008 | 0.0001 | 0.0148 | 0.0002 | 0.0026 | 0.0019 | 0.0003 | 0.0034 | 0.0003 | 0.001 | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0004 | 0.0004 |
Vacuole | 0.0081 | 0.0106 | 0.0049 | 0.0053 | 0.01 | 0.0039 | 0.009 | 0.008 | 0.0036 | 0.0041 | 0.0055 | 0.0042 | 0.0025 | 0.0053 | 0.0041 | 0.0035 | 0.0022 | 0.0025 |
Vacuole Periphery | 0.0009 | 0.0004 | 0.0004 | 0.0002 | 0.002 | 0.0004 | 0.0019 | 0.0008 | 0.0003 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 23.7028 | 24.8421 | 24.94 | 33.264 | 18.0151 | 22.8728 | 33.6135 | 40.8862 | 33.3148 | 38.6303 |
Translational Efficiency | 1.0269 | 0.6987 | 0.7175 | 0.5439 | 0.9193 | 1.1059 | 0.7519 | 0.5073 | 0.7307 | 0.6453 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
108 | 924 | 288 | 1536 | 1636 | 1079 | 2160 | 1758 | 1744 | 2003 | 2448 | 3294 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1100.49 | 1001.00 | 1353.24 | 1132.25 | 931.89 | 959.84 | 1078.43 | 1127.37 | 942.33 | 978.83 | 1110.76 | 1129.65 |
Standard Deviation | 158.22 | 175.19 | 159.26 | 144.56 | 153.93 | 128.22 | 146.04 | 153.56 | 159.46 | 153.09 | 172.17 | 149.45 |
Intensity Change Log 2 | — | -0.136704 | 0.298272 | 0.041047 | — | 0.042634 | 0.210701 | 0.274730 | — | -0.051698 | 0.258777 | 0.152910 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002907 | 0.002738 | 0.005691 | 0.010397 | 0.001125 | 0.000398 | 0.004932 | 0.011062 | 0.001235 | 0.001477 | 0.005021 | 0.010752 |
Bud Neck | 0.003529 | 0.001547 | 0.003233 | 0.011328 | 0.000780 | 0.000793 | 0.004261 | 0.009958 | 0.000950 | 0.001141 | 0.004140 | 0.010597 |
Bud Site | 0.018427 | 0.010355 | 0.011637 | 0.044759 | 0.007435 | 0.002222 | 0.011628 | 0.038512 | 0.008116 | 0.005974 | 0.011629 | 0.041425 |
Cell Periphery | 0.003766 | 0.004708 | 0.004279 | 0.010211 | 0.007704 | 0.003539 | 0.007424 | 0.007388 | 0.007460 | 0.004078 | 0.007054 | 0.008704 |
Cytoplasm | 0.086041 | 0.073891 | 0.029696 | 0.135976* | 0.041968 | 0.094177 | 0.134269* | 0.149761* | 0.044697 | 0.084819 | 0.121966* | 0.143333* |
Cytoplasmic Foci | 0.043364 | 0.021707 | 0.005262 | 0.033226 | 0.012615 | 0.015924 | 0.009718 | 0.034567 | 0.014519 | 0.018592 | 0.009194 | 0.033941 |
Eisosomes | 0.000781 | 0.000623 | 0.000174 | 0.002001 | 0.000570 | 0.000763 | 0.001916 | 0.002175 | 0.000583 | 0.000699 | 0.001711 | 0.002094 |
Endoplasmic Reticulum | 0.539077* | 0.805234* | 0.830292* | 0.512067* | 0.878851* | 0.809561* | 0.746638* | 0.517072* | 0.857810* | 0.807565* | 0.756480* | 0.514738* |
Endosome | 0.016406 | 0.012057 | 0.011643 | 0.022753 | 0.004470 | 0.005987 | 0.005511 | 0.024434 | 0.005209 | 0.008787 | 0.006232 | 0.023650 |
Golgi | 0.008044 | 0.011014 | 0.001488 | 0.015865 | 0.008521 | 0.014302 | 0.007105 | 0.013823 | 0.008492 | 0.012785 | 0.006444 | 0.014775 |
Lipid Particles | 0.028039 | 0.019413 | 0.015415 | 0.067865 | 0.008521 | 0.022636 | 0.031019 | 0.087231 | 0.009730 | 0.021149 | 0.029184 | 0.078200 |
Mitochondria | 0.001702 | 0.002596 | 0.000098 | 0.003361 | 0.007175 | 0.005399 | 0.003731 | 0.003499 | 0.006836 | 0.004106 | 0.003304 | 0.003435 |
Mitotic Spindle | 0.003179 | 0.000359 | 0.000222 | 0.009530 | 0.000140 | 0.000616 | 0.000120 | 0.003657 | 0.000328 | 0.000498 | 0.000132 | 0.006396 |
None | 0.000015 | 0.002568 | 0.000299 | 0.000910 | 0.004299 | 0.002235 | 0.007282 | 0.001852 | 0.004034 | 0.002388 | 0.006460 | 0.001412 |
Nuclear Periphery | 0.001607 | 0.001587 | 0.003334 | 0.001044 | 0.000547 | 0.000653 | 0.000183 | 0.001828 | 0.000613 | 0.001084 | 0.000554 | 0.001463 |
Nuclear Periphery Foci | 0.024419 | 0.011340 | 0.042968 | 0.018069 | 0.002171 | 0.004280 | 0.002715 | 0.016751 | 0.003549 | 0.007537 | 0.007451 | 0.017365 |
Nucleolus | 0.000043 | 0.000114 | 0.000006 | 0.000155 | 0.000054 | 0.000088 | 0.000116 | 0.000200 | 0.000053 | 0.000100 | 0.000103 | 0.000179 |
Nucleus | 0.007972 | 0.003571 | 0.001936 | 0.013870 | 0.001230 | 0.004401 | 0.007358 | 0.015494 | 0.001647 | 0.004018 | 0.006720 | 0.014737 |
Peroxisomes | 0.001011 | 0.001884 | 0.000138 | 0.003530 | 0.007034 | 0.002432 | 0.004903 | 0.004460 | 0.006661 | 0.002179 | 0.004343 | 0.004026 |
Vacuole | 0.195013 | 0.010113 | 0.031888 | 0.080051 | 0.002637 | 0.007711 | 0.008741 | 0.054454 | 0.014550 | 0.008819 | 0.011464 | 0.066390 |
Vacuole Periphery | 0.014657 | 0.002581 | 0.000300 | 0.003033 | 0.002152 | 0.001882 | 0.000427 | 0.001824 | 0.002926 | 0.002204 | 0.000412 | 0.002387 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.15 | -1.03 | -5.81 | -6.35 | -1.68 | 3.70 | -5.20 | -10.27 | -11.21 | -5.12 | -0.63 | -5.27 | -13.57 | -12.25 | -5.84 |
Bud Neck | 2.78 | 0.26 | -7.45 | -10.69 | -7.79 | -0.06 | -5.28 | -11.30 | -11.37 | -5.81 | -0.75 | -5.41 | -15.71 | -15.59 | -8.39 |
Bud Site | 1.20 | 0.97 | -2.80 | -9.99 | -7.70 | 4.65 | -2.84 | -10.96 | -13.49 | -9.36 | 1.64 | -2.31 | -14.67 | -16.45 | -13.27 |
Cell Periphery | -0.73 | -0.35 | -4.54 | -3.95 | -3.50 | 3.79 | 0.26 | 0.47 | -4.29 | 0.31 | 3.26 | 0.40 | -0.86 | -5.76 | -1.85 |
Cytoplasm | 0.84 | 3.75 | -3.48 | -9.65 | -15.77 | -10.59 | -21.36 | -21.33 | -9.03 | -2.84 | -10.14 | -18.93 | -24.79 | -13.14 | -4.88 |
Cytoplasmic Foci | 2.27 | 3.86 | 1.10 | -4.41 | -12.41 | -1.63 | 1.85 | -10.57 | -7.83 | -12.44 | -2.15 | 3.48 | -11.17 | -8.71 | -16.53 |
Eisosomes | 0.55 | 2.94 | -4.11 | -4.81 | -8.66 | -0.84 | -5.46 | -6.52 | -6.00 | -0.86 | -0.58 | -5.02 | -7.05 | -7.63 | -1.56 |
Endoplasmic Reticulum | -6.61 | -6.86 | 0.93 | 23.28 | 19.30 | 7.08 | 15.95 | 36.04* | 25.61 | 22.41 | 5.69 | 12.11 | 40.42 | 34.60 | 29.70 |
Endosome | 1.73 | 1.85 | -2.29 | -6.21 | -6.75 | -1.94 | -2.04 | -14.88 | -12.55 | -13.95 | -4.63 | -2.05 | -19.12 | -13.29 | -17.95 |
Golgi | -0.91 | 1.96 | -2.02 | -2.07 | -9.10 | -3.17 | 1.17 | -3.37 | 0.52 | -6.12 | -2.98 | 1.77 | -4.16 | -1.07 | -8.20 |
Lipid Particles | 1.41 | 1.90 | -5.93 | -12.16 | -12.12 | -6.98 | -10.42 | -19.96 | -15.13 | -12.95 | -6.85 | -9.82 | -25.12 | -19.39 | -15.57 |
Mitochondria | -0.79 | 1.41 | -1.30 | -0.94 | -6.21 | 1.25 | 3.66 | 3.75 | 1.70 | 0.63 | 2.57 | 4.00 | 3.83 | 1.10 | -0.08 |
Mitotic Spindle | 1.63 | 1.71 | -3.06 | -5.93 | -6.02 | -1.40 | 0.20 | -5.71 | -4.53 | -5.73 | -0.70 | 1.53 | -7.57 | -7.30 | -7.84 |
None | -3.57 | -1.74 | -4.63 | 2.30 | -2.34 | 1.83 | -2.27 | 2.31 | 0.56 | 5.37 | 1.62 | -2.02 | 2.83 | 1.90 | 6.10 |
Nuclear Periphery | 0.07 | -1.24 | 0.88 | 0.42 | 1.62 | -0.66 | 2.51 | -2.65 | -2.52 | -3.52 | -1.18 | 0.29 | -3.22 | -1.08 | -3.34 |
Nuclear Periphery Foci | 1.93 | -2.15 | 0.76 | -4.06 | 4.70 | -3.09 | -1.15 | -11.70 | -9.66 | -11.42 | -4.26 | -4.40 | -13.23 | -9.16 | -9.27 |
Nucleolus | -2.20 | 3.04 | -6.05 | -1.50 | -9.61 | -1.64 | -3.82 | -6.93 | -4.43 | -3.96 | -2.47 | -3.40 | -8.60 | -4.15 | -4.98 |
Nucleus | 1.77 | 2.39 | -2.55 | -10.26 | -11.91 | -7.01 | -9.32 | -14.61 | -10.75 | -7.25 | -6.32 | -8.40 | -18.64 | -14.84 | -9.54 |
Peroxisomes | -2.75 | 4.17 | -5.99 | -3.68 | -9.31 | 5.84 | 2.53 | 3.11 | -3.68 | 0.77 | 6.32 | 2.98 | 3.65 | -5.11 | 0.74 |
Vacuole | 6.99 | 6.04 | 3.79 | -19.10 | -9.92 | -4.89 | -7.90 | -19.11 | -16.86 | -16.77 | 2.78 | 1.31 | -18.97 | -25.34 | -23.93 |
Vacuole Periphery | 1.24 | 1.49 | 1.21 | -0.47 | -4.94 | 0.37 | 4.32 | 0.68 | 0.14 | -3.59 | 0.88 | 3.59 | 0.80 | -0.19 | -6.00 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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