Standard name
Human Ortholog
Description Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.84 0.97 0.98 0.95 0.92 0.74 0.75 – 0.62 0.59 0.59 0.58 0.99 1.0 0.99 0.89 0.88 0.93 0.83 0.91 0.89 0.91 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.22 0 – 0.26 0.26 0.23 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.13 0.1 0.23 – 0.22 0.22 0.23 0.24 0 0 0 0.19 0.22 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 0 0 0 1 1 2 0 1
Bud 2 0 0 1 0 8 2 – 3 4 5 0 0 0 0 0 0 0 1 0 0 2 8 7
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 3 1 2 2 1 8 – 1 1 7 8 0 0 3 4 3 8 0 0 0 0 0 0
Cytoplasm 187 208 278 269 292 148 272 – 220 173 224 265 346 295 393 140 134 198 245 197 267 178 327 247
Endoplasmic Reticulum 0 2 3 1 0 1 1 – 0 2 5 10 1 1 2 1 1 1 2 0 1 3 1 1
Endosome 3 0 1 0 1 0 2 – 5 0 1 2 0 0 0 0 1 0 9 1 6 0 1 1
Golgi 0 0 1 2 0 1 0 – 0 0 0 0 0 0 1 0 0 1 4 0 2 1 0 3
Mitochondria 11 5 1 7 5 44 14 – 92 77 89 108 0 0 0 2 2 2 5 4 5 1 8 4
Nucleus 7 0 2 2 0 1 14 – 12 12 26 20 0 0 1 2 2 0 1 1 0 0 0 0
Nuclear Periphery 9 0 0 0 0 1 0 – 2 1 1 2 0 0 0 0 0 0 2 0 0 1 1 1
Nucleolus 2 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 2 0 6 0 3 2
Vac/Vac Membrane 10 2 2 11 40 19 82 – 77 63 88 109 4 1 3 30 34 20 8 2 2 1 2 2
Unique Cell Count 223 215 285 283 317 200 364 352 291 379 459 350 296 396 158 153 212 296 218 299 197 361 279
Labelled Cell Count 233 220 289 295 340 224 396 414 333 446 527 351 297 403 179 177 230 296 218 299 197 361 279


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.4 6.2 6.2 7.4 5.4 7.1 – 6.4 6.2 7.7 6.9 7.1 7.0 7.3 9.9 10.4 10.2 6.3 6.0 5.9
Std Deviation (1e-4) 0.9 0.9 0.9 1.3 1.5 1.4 1.6 – 1.4 1.7 21.2 1.4 1.4 1.3 1.3 1.8 2.6 1.8 2.9 2.5 2.4
Intensity Change (Log2) – – – 0.01 0.27 -0.19 0.2 – 0.05 0.01 0.33 0.17 0.21 0.18 0.25 0.68 0.76 0.73 0.03 -0.05 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -3.0 -7.8 -8.0 – -10.6 -11.1 -11.4 -11.9 1.3 2.2 1.8 -3.9 -4.2 -2.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.1 2.9 – 9.2 9.2 8.6 8.7 0 0 0 0 0 0
Nucleus 0 0 0 2.6 – 2.3 2.7 3.9 2.9 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.5 5.7 4.7 8.2 – 8.1 7.9 8.4 8.6 0 0 0 7.1 7.8 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1295 5.2129 3.814 4.1552 3.3286 3.7813 4.4285 6.1799 5.3517 4.6232 3.9362 5.6091 8.3118 9.0535 8.3323 8.6085 7.8372 8.3008
Actin 0.023 0.0003 0.0095 0.0001 0.0199 0.0026 0.0431 0.0009 0.0105 0.0007 0.0057 0.0013 0.0112 0.0003 0.018 0.0018 0.0005 0.0004
Bud 0.0011 0.0001 0.0005 0.0001 0.002 0.0001 0.0008 0.0047 0.0009 0.0001 0.0008 0.0023 0.0004 0.0001 0.0008 0.0001 0.0001 0.0001
Bud Neck 0.0035 0.0002 0.001 0.0005 0.0007 0.0016 0.0009 0.0002 0.0015 0.0003 0.0007 0.0007 0.0041 0.0002 0.0005 0.0003 0.0004 0.0008
Bud Periphery 0.0014 0 0.0004 0 0.0029 0.0001 0.0009 0.0028 0.0017 0 0.0013 0.0008 0.0004 0 0.0004 0 0 0
Bud Site 0.0027 0.0003 0.001 0 0.004 0.0001 0.0034 0.0108 0.0094 0.0001 0.0007 0.0003 0.0042 0.0005 0.0021 0.0002 0.0001 0.0001
Cell Periphery 0.0003 0 0.0001 0 0.0001 0 0.0002 0.0006 0.0012 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0
Cytoplasm 0.8256 0.9712 0.9089 0.9711 0.8652 0.9348 0.5407 0.9359 0.8853 0.9866 0.779 0.9145 0.9159 0.9871 0.9251 0.9815 0.9676 0.9614
Cytoplasmic Foci 0.0249 0.0026 0.0103 0.0025 0.015 0.0072 0.0652 0.0038 0.0131 0.0017 0.0218 0.0101 0.0146 0.0017 0.0026 0.0032 0.0046 0.0038
Eisosomes 0.0002 0 0 0 0.0001 0 0.0004 0 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0048 0.002 0.0025 0.0019 0.0041 0.0033 0.0087 0.0007 0.0026 0.0016 0.0041 0.0015 0.0038 0.0005 0.004 0.0014 0.0022 0.0014
Endosome 0.0316 0.0023 0.009 0.0054 0.0214 0.0112 0.0857 0.0003 0.0188 0.0007 0.0181 0.0106 0.005 0.0003 0.0029 0.001 0.0013 0.0036
Golgi 0.008 0.0001 0.0028 0.0001 0.0073 0.0018 0.0286 0.0001 0.0185 0 0.0032 0.0041 0.0021 0 0.0014 0.0001 0.0001 0.0017
Lipid Particles 0.0107 0 0.0029 0 0.0047 0.0032 0.0332 0.0001 0.0036 0 0.0032 0.0053 0.0023 0 0.0015 0 0 0.0003
Mitochondria 0.0112 0.0003 0.0019 0.0002 0.0058 0.0011 0.0163 0.0177 0.0083 0.0001 0.0527 0.0021 0.0006 0.0001 0.0011 0.0001 0.0001 0.0002
None 0.0127 0.0033 0.0155 0.0036 0.0101 0.0079 0.0378 0.0164 0.0027 0.0026 0.0375 0.0203 0.0168 0.0031 0.0133 0.004 0.0158 0.0101
Nuclear Periphery 0.0047 0.004 0.0032 0.0029 0.006 0.0032 0.0374 0.0005 0.0017 0.0009 0.023 0.0117 0.0029 0.0004 0.003 0.0008 0.0006 0.0009
Nucleolus 0.0006 0 0.0007 0 0.0003 0.0001 0.0039 0.0002 0.0011 0 0.0015 0.0008 0.0001 0 0.0006 0 0 0
Nucleus 0.0129 0.0114 0.0115 0.0095 0.0155 0.0144 0.0337 0.0028 0.0039 0.0038 0.0118 0.0059 0.008 0.0051 0.0174 0.0045 0.0054 0.0142
Peroxisomes 0.0109 0 0.0121 0 0.0052 0.0025 0.0109 0.0001 0.0035 0 0.0099 0.0012 0.0037 0 0.001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0028 0.0011 0.0043 0.0003 0.0074 0.0027 0.0401 0.001 0.0004 0.0004 0.016 0.002 0.003 0.0003 0.0035 0.0005 0.0005 0.0004
Vacuole 0.0043 0.0006 0.0015 0.0016 0.0019 0.0016 0.0046 0.0005 0.0092 0.0003 0.0043 0.0017 0.0005 0.0002 0.0005 0.0003 0.0003 0.0003
Vacuole Periphery 0.002 0.0002 0.0005 0.0004 0.0005 0.0004 0.0036 0.0001 0.002 0.0001 0.0045 0.0029 0.0002 0 0.0002 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.28 62.3571 67.3042 86.8822 83.2468 66.5479 77.3786 85.5915 89.861 79.0595
Translational Efficiency 2.9522 2.3873 2.0401 2.1702 1.9052 2.7333 1.7351 2.2361 2.1824 2.0082

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate
Localization
Cell Percentages cytoplasm (67%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ysa1

Ysa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ysa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available