Standard name
Human Ortholog
Description Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.6 0.65 0.17 0.17 0 0 0 0.07 0 0.08 0.06 0.06 0.35 0.39 0.45 0.6 0.6 0.59 0.14 0.22 0 0.09 0.12 0.23
Cytoplasm 0.63 0.53 0.92 0.94 0.99 0.98 0.96 0.93 0.94 0.93 0.94 0.94 0.75 0.69 0.65 0.58 0.55 0.54 0.79 0.74 0.94 0.84 0.81 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 2 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 92 11 27 33 12 9 12 28 12 22 14 18 53 88 116 86 95 72 32 18 1 17 26 62
Cytoplasm 97 9 145 184 302 359 325 349 268 264 225 267 115 158 166 84 87 66 180 63 148 159 182 197
Endoplasmic Reticulum 0 0 0 0 0 0 3 5 10 5 9 6 0 0 0 2 2 1 6 1 2 4 4 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 1 0
Nucleolus 0 0 0 1 0 0 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 1 1 1
Unique Cell Count 153 17 158 196 305 365 339 376 286 285 240 285 153 228 257 144 159 122 230 86 158 189 226 277
Labelled Cell Count 189 20 172 220 314 369 340 385 292 292 249 291 169 247 285 172 184 139 230 86 158 189 226 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 650.2 642.0 476.4 481.6 395.4 297.8 284.2 284.5 261.6 251.1 238.8 238.6 552.9 556.9 582.4 678.7 670.0 679.0 622.3 599.2 615.2
Std Deviation (1e-4) 102.9 157.9 97.9 84.9 63.9 67.9 63.7 73.8 60.2 59.0 58.8 57.5 111.6 119.6 117.7 97.4 120.2 114.4 113.0 156.2 163.0
Intensity Change (Log2) – – – 0.02 -0.27 -0.68 -0.75 -0.74 -0.86 -0.92 -1.0 -1.0 0.21 0.23 0.29 0.51 0.49 0.51 0.39 0.33 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -4.8 -6.1 -5.2 -3.3 -4.6 -3.0 -3.6 -3.6 3.5 4.5 5.8 7.6 7.8 7.3
Cytoplasm 0.8 4.0 3.7 1.9 0.4 0.8 0.3 0.8 0.8 -4.0 -5.3 -6.2 -6.8 -7.4 -7.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0021 0.0007 0.0009 0.0046 0.0054 0.0062 0.0027 0.0011 0.0851 0.0001 0.0001 0.0168 – – – – – –
Bud 0.0035 0.0033 0.0039 0.0024 0.0028 0.0062 0.0072 0.0044 0.0247 0.0001 0.0065 0.0046 – – – – – –
Bud Neck 0.0018 0.0011 0.0019 0.002 0.0021 0.0046 0.0008 0.0005 0.0016 0.0002 0.0002 0.0036 – – – – – –
Bud Periphery 0.0051 0.0018 0.0034 0.0017 0.0034 0.007 0.0109 0.0009 0.0711 0 0.0018 0.0051 – – – – – –
Bud Site 0.0017 0.002 0.0027 0.0005 0.0072 0.0012 0.008 0.001 0.0063 0 0.0011 0.0019 – – – – – –
Cell Periphery 0.1276 0.0358 0.1031 0.014 0.0188 0.0569 0.0253 0.0033 0.0203 0 0.0066 0.0136 – – – – – –
Cytoplasm 0.3408 0.7569 0.5086 0.2465 0.4253 0.3694 0.0138 0.7472 0.1442 0.984 0.0153 0.4502 – – – – – –
Cytoplasmic Foci 0.0036 0.0003 0.0017 0.0014 0.0201 0.0026 0.0498 0.0004 0.1536 0.0001 0.0104 0.0026 – – – – – –
Eisosomes 0.0063 0.0003 0.0009 0.0028 0.0001 0.0019 0.0027 0 0.0044 0 0.0005 0.0036 – – – – – –
Endoplasmic Reticulum 0.0202 0.0023 0.0022 0.002 0.0249 0.0108 0.0093 0.0003 0.0062 0 0.0001 0.0076 – – – – – –
Endosome 0.001 0.0003 0.0008 0.0008 0.0473 0.0025 0.053 0.0004 0.0475 0 0.0004 0.0149 – – – – – –
Golgi 0.0002 0.0001 0.0001 0.0002 0.0242 0.0006 0.0245 0 0.0268 0 0 0.0022 – – – – – –
Lipid Particles 0.0034 0.0005 0.0025 0.0005 0.0028 0.0037 0.0225 0 0.0168 0 0.0006 0.0032 – – – – – –
Mitochondria 0.0408 0.0071 0.0224 0.2496 0.0154 0.0775 0.2608 0.0527 0.0972 0.0004 0.0766 0.0936 – – – – – –
None 0.0097 0.0021 0.0065 0.038 0.0046 0.0064 0.0326 0.0019 0.039 0.0001 0.0384 0.0238 – – – – – –
Nuclear Periphery 0.0917 0.0252 0.0275 0.0892 0.074 0.1019 0.0151 0.0076 0.0085 0.0013 0.0015 0.0464 – – – – – –
Nucleolus 0.0726 0.0079 0.0605 0.0438 0.0095 0.0401 0.2834 0.0035 0.1077 0 0.553 0.0454 – – – – – –
Nucleus 0.0978 0.0879 0.0872 0.2399 0.1471 0.1433 0.0917 0.1362 0.0383 0.013 0.2365 0.0789 – – – – – –
Peroxisomes 0.0009 0.0002 0.0007 0.0031 0.0013 0.0011 0.0056 0.0006 0.0118 0 0.0012 0.0018 – – – – – –
Punctate Nuclear 0.0006 0.0002 0.0003 0.0008 0.0007 0.0007 0.0062 0.0001 0.0033 0 0.0012 0.0031 – – – – – –
Vacuole 0.1362 0.0544 0.1319 0.0219 0.0904 0.0931 0.035 0.0221 0.0564 0.0003 0.026 0.069 – – – – – –
Vacuole Periphery 0.0323 0.0095 0.0301 0.0342 0.0727 0.0624 0.0389 0.0161 0.0291 0.0003 0.0218 0.108 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16958.533 17918.809 18171.3034 15684.7149 17595.3859 17032.8353 16829.5697 15729.5032 16756.5084 14640.5285
Translational Efficiency 0.6565 0.645 0.6438 0.6135 0.6701 0.6771 0.7227 0.821 0.7059 0.842

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1672 1210 271 93 1299 1755 773 16 2971 2965 1044 109

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 42260.24 43100.40 52199.98 56386.06 39438.84 38239.42 51863.65 382.02 41026.65 40223.16 51950.95 48165.28
Standard Deviation 7323.02 7984.11 5803.78 7368.42 7703.73 8914.02 5037.54 8.67 7621.46 8874.39 5249.27 20955.65
Intensity Change Log 2 — 0.028400 0.304748 0.416038 — -0.044556 0.395107 -6.689825 — -0.006358 0.349074 -0.525240

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000072 0.000021 0.000014 0.000027 0.000065 0.000021 0.000049 0.000034 0.000068 0.000021 0.000019
Bud Neck 0.000121 0.000263 0.000348 0.000042 0.000109 0.000386 0.000114 0.000002 0.000116 0.000336 0.000174 0.000036
Bud Site 0.000216 0.000656 0.000038 0.000037 0.000234 0.000580 0.000035 0.000011 0.000224 0.000611 0.000036 0.000033
Cell Periphery 0.139104* 0.197956* 0.228565* 0.185656* 0.141649* 0.114580* 0.190148* 0.000206 0.140217* 0.148605* 0.200120* 0.158434*
Cytoplasm 0.433572* 0.226616* 0.010270 0.012324 0.418025* 0.444200* 0.024063 0.000026 0.426774* 0.355405* 0.020483 0.010519
Cytoplasmic Foci 0.000816 0.000994 0.000073 0.003758 0.000842 0.001701 0.000020 0.000005 0.000828 0.001412 0.000034 0.003207
Eisosomes 0.000007 0.000013 0.000096 0.000017 0.000006 0.000007 0.000035 0.000237 0.000006 0.000009 0.000051 0.000049
Endoplasmic Reticulum 0.001852 0.000893 0.000097 0.000008 0.001274 0.001465 0.000069 0.000001 0.001599 0.001232 0.000076 0.000007
Endosome 0.000087 0.000079 0.000016 0.003163 0.000100 0.000138 0.000005 0.000000 0.000093 0.000114 0.000007 0.002699
Golgi 0.010283 0.007751 0.001123 0.003051 0.013770 0.008252 0.000204 0.000001 0.011808 0.008047 0.000443 0.002604
Lipid Particles 0.014603 0.012070 0.003793 0.000342 0.014708 0.012012 0.000928 0.000009 0.014649 0.012035 0.001672 0.000294
Mitochondria 0.000133 0.000105 0.000010 0.000082 0.000074 0.000189 0.000004 0.000007 0.000107 0.000155 0.000005 0.000071
Mitotic Spindle 0.000158 0.000130 0.000039 0.000035 0.000171 0.000219 0.000011 0.000125 0.000164 0.000182 0.000018 0.000048
None 0.000637 0.000646 0.000012 0.000001 0.001116 0.001949 0.000003 0.999186* 0.000847 0.001417 0.000005 0.146670*
Nuclear Periphery 0.002331 0.001931 0.001085 0.000323 0.002191 0.001484 0.000854 0.000000 0.002270 0.001666 0.000914 0.000276
Nuclear Periphery Foci 0.004215 0.003847 0.000564 0.000134 0.006055 0.004675 0.000243 0.000001 0.005020 0.004337 0.000327 0.000114
Nucleolus 0.000091 0.000109 0.000015 0.000004 0.000051 0.000256 0.000011 0.000001 0.000073 0.000196 0.000012 0.000004
Nucleus 0.004446 0.003071 0.000928 0.000130 0.003456 0.006699 0.000650 0.000003 0.004013 0.005218 0.000722 0.000111
Peroxisomes 0.000153 0.000073 0.000003 0.000251 0.000061 0.000099 0.000005 0.000131 0.000113 0.000088 0.000005 0.000234
Vacuole 0.356589* 0.524535* 0.747021* 0.790169* 0.354231* 0.376713* 0.781158* 0.000000 0.355558* 0.437038* 0.772297* 0.674181*
Vacuole Periphery 0.030546 0.018190 0.005882 0.000459 0.041851 0.024333 0.001421 0.000000 0.035489 0.021826 0.002579 0.000391

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.20 3.65 3.58 5.16 1.06 -5.08 1.87 -1.22 1.64 -1.55 -5.63 4.24 2.43 6.23 0.38
Bud Neck -2.91 -2.01 4.35 4.51 2.67 -4.99 -0.23 9.31 6.44 7.31 -5.69 -1.81 5.49 7.21 4.03
Bud Site -1.68 5.28 4.61 2.35 0.14 -3.77 4.98 6.06 5.49 1.67 -3.65 6.99 6.23 5.04 0.21
Cell Periphery -10.15 -10.75 -3.04 0.74 2.52 5.49 -8.11 32.82* 32.21* 44.87* -1.65 -12.98 -1.29 -0.84 2.80
Cytoplasm 16.45 45.18 45.42 23.58 -0.46 -2.05 37.53 41.05 50.21 9.79 7.43 57.87 59.08 49.55 3.59
Cytoplasmic Foci -0.64 8.25 -0.78 -0.72 -0.98 -1.92 9.17 9.36 3.36 2.64 -2.04 12.62 -0.74 -0.49 -0.99
Eisosomes -5.03 -5.50 -3.41 -1.26 4.78 -0.79 -6.82 -3.06 -3.05 -2.67 -3.83 -8.37 -3.21 -2.99 0.09
Endoplasmic Reticulum 3.92 8.23 8.71 7.65 3.83 -0.71 6.45 6.83 9.50 5.24 2.11 10.51 11.03 11.93 5.97
Endosome 0.58 9.98 -0.97 -0.97 -1.00 -2.81 10.93 11.47 10.18 9.35 -2.58 16.22 -0.97 -0.96 -1.00
Golgi 2.53 12.82 2.34 1.52 -0.62 5.71 16.92 17.20 14.41 4.96 5.57 21.87 3.50 2.06 -0.83
Lipid Particles 1.94 7.98 16.32 12.59 3.64 1.86 12.02 12.88 12.72 10.01 2.70 17.21 20.44 17.33 5.14
Mitochondria 0.91 6.53 0.66 0.36 -0.87 -3.32 7.63 6.89 4.93 -1.07 -2.29 9.02 0.55 1.42 -0.94
Mitotic Spindle 2.12 8.18 4.40 3.39 0.20 -1.09 13.42 1.42 1.68 -3.77 -0.84 18.46 4.76 3.40 -1.28
None -0.06 11.43 11.68 3.41 2.95 -1.96 6.25 -3324.78* -2176.84* -4130.48* -2.23 10.06 -4.29 -4.27 -4.31
Nuclear Periphery 2.69 7.33 15.62 14.84 5.95 4.76 9.68 16.86 20.36 18.53 5.82 13.75 20.09 18.43 9.60
Nuclear Periphery Foci 0.87 12.90 16.54 11.23 3.25 2.33 13.80 14.41 13.27 9.24 1.84 20.03 21.21 16.95 4.80
Nucleolus -0.36 3.09 3.54 2.09 5.13 -1.27 18.29 22.71* 1.55 11.07 -1.25 4.45 5.02 1.92 8.73
Nucleus 1.74 5.42 7.17 6.45 3.42 -5.25 6.21 7.85 10.56 6.92 -3.17 8.17 9.96 12.08 6.44
Peroxisomes 3.08 6.05 -0.40 -0.72 -0.99 -1.52 7.58 -1.98 -0.84 -3.63 1.29 7.57 -0.57 -0.69 -1.08
Vacuole -15.65 -36.20 -23.12 -13.87 -2.26 -2.05 -46.03 44.06* 52.16* 170.75* -10.59 -62.73* -10.21 -7.60 3.15
Vacuole Periphery 5.44 12.05 17.64 11.89 4.91 6.01 15.95 16.55 15.65 9.36 7.55 21.91 23.88 19.35 6.67
Standard name
Human Ortholog
Description Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tef2

Tef2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tef2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available