Standard name | |
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Human Ortholog | |
Description | U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 3 | 10 | 5 | 8 | 6 | 7 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 4 | 5 | 11 | 14 | 19 | 22 | 24 | 16 | 18 | 11 | 7 | 21 | 45 | 80 | 22 | 10 | 21 | 0 | 0 | 0 | 2 | 3 | 5 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 3 | 3 | 0 | 5 | 3 | 3 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 2 | 2 | 0 | 1 | 0 | 0 | 3 |
Mitochondria | 33 | 21 | 2 | 7 | 9 | 44 | 134 | 77 | 111 | 130 | 124 | 125 | 7 | 4 | 0 | 6 | 5 | 9 | 1 | 0 | 1 | 1 | 6 | 7 |
Nucleus | 112 | 47 | 104 | 181 | 336 | 317 | 362 | 453 | 238 | 327 | 221 | 406 | 315 | 429 | 413 | 208 | 141 | 167 | 98 | 66 | 96 | 213 | 297 | 290 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 0 | 2 | 4 | 4 | 2 | 1 | 1 | 5 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 24 | 3 | 7 | 3 | 2 | 4 | 16 | 13 | 22 | 23 | 35 | 34 | 1 | 8 | 5 | 6 | 3 | 6 | 15 | 2 | 4 | 21 | 29 | 32 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 2 | 13 | 4 | 7 | 15 | 5 | 2 | 2 | 7 | 4 | 5 | 8 | 6 | 1 | 0 | 0 | 1 | 1 | 2 | 8 | 5 |
Vac/Vac Membrane | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 4 | 2 | 1 | 2 | 5 | 2 | 1 | 2 | 13 | 8 | 8 | 2 | 5 | 6 | 4 | 30 | 32 |
Unique Cell Count | 123 | 55 | 113 | 192 | 363 | 346 | 440 | 510 | 303 | 400 | 280 | 483 | 340 | 480 | 506 | 245 | 162 | 212 | 125 | 80 | 122 | 254 | 392 | 400 |
Labelled Cell Count | 171 | 76 | 121 | 205 | 383 | 397 | 558 | 595 | 408 | 514 | 409 | 599 | 352 | 495 | 520 | 270 | 176 | 223 | 125 | 80 | 122 | 254 | 392 | 400 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 4.8 | 6.1 | 5.9 | 5.6 | 4.8 | 4.5 | 5.2 | 4.4 | 4.6 | 4.4 | 4.7 | 6.5 | 6.5 | 6.5 | 8.8 | 8.4 | 8.5 | 5.9 | 6.0 | 7.1 |
Std Deviation (1e-4) | 0.9 | 1.4 | 1.3 | 1.4 | 1.4 | 1.3 | 1.3 | 1.7 | 1.4 | 1.1 | 1.3 | 1.0 | 1.4 | 1.3 | 1.6 | 2.5 | 2.0 | 2.8 | 1.2 | 1.2 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.12 | -0.35 | -0.45 | -0.22 | -0.47 | -0.43 | -0.49 | -0.37 | 0.08 | 0.08 | 0.09 | 0.53 | 0.45 | 0.47 | -0.04 | -0.03 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8174 | 1.2414 | 1.1263 | 0.7798 | 0.4039 | 0.9889 | 0.4364 | 1.1184 | 0.7405 | 0.3228 | 0.3891 | 0.804 | 0.5589 | 0.7477 | 0.4628 | 0.6022 | 0.7898 | 0.6056 |
Actin | 0.019 | 0 | 0.0017 | 0.0058 | 0.0005 | 0.0006 | 0.0278 | 0 | 0.0006 | 0.0086 | 0.0002 | 0 | 0.0137 | 0 | 0.0194 | 0 | 0.0534 | 0 |
Bud | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0007 | 0 |
Bud Neck | 0.0035 | 0.0001 | 0.0048 | 0.0005 | 0.0007 | 0.0007 | 0.0069 | 0.0001 | 0.0005 | 0.0123 | 0.0009 | 0.0012 | 0.0058 | 0.0002 | 0.0003 | 0 | 0.0012 | 0.0008 |
Bud Periphery | 0.0013 | 0 | 0.0005 | 0.0008 | 0.0024 | 0 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0.0019 | 0 |
Bud Site | 0.0034 | 0 | 0.0027 | 0.0016 | 0.0011 | 0.0001 | 0.0046 | 0.0001 | 0.0008 | 0.005 | 0.0001 | 0 | 0.0025 | 0.0003 | 0.0012 | 0 | 0.0008 | 0 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0.0003 | 0.001 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Cytoplasm | 0.0042 | 0.0009 | 0.001 | 0.0015 | 0.0037 | 0.0003 | 0.0069 | 0.0034 | 0.0002 | 0.0036 | 0.0009 | 0.0001 | 0.0027 | 0 | 0.0018 | 0 | 0.0008 | 0.0001 |
Cytoplasmic Foci | 0.0205 | 0 | 0.0024 | 0.003 | 0.0075 | 0.0016 | 0.0238 | 0.0001 | 0.0109 | 0.0244 | 0.0071 | 0 | 0.0105 | 0.0004 | 0.0046 | 0 | 0.0001 | 0 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0023 | 0 | 0.0005 | 0.0025 | 0.003 | 0 | 0.0018 | 0.0001 | 0 | 0.0005 | 0.0001 | 0 | 0.0008 | 0 | 0.0008 | 0 | 0.0006 | 0 |
Endosome | 0.0251 | 0 | 0.0023 | 0.0192 | 0.0044 | 0.0007 | 0.0267 | 0.0001 | 0.0028 | 0.0224 | 0.0007 | 0 | 0.0067 | 0 | 0.0062 | 0 | 0.0013 | 0 |
Golgi | 0.0042 | 0 | 0.0018 | 0.006 | 0.0006 | 0.0005 | 0.0126 | 0 | 0.0019 | 0.0041 | 0.0009 | 0 | 0.0029 | 0 | 0.003 | 0 | 0.0022 | 0 |
Lipid Particles | 0.007 | 0 | 0.0107 | 0.009 | 0.0056 | 0.0008 | 0.0336 | 0 | 0.011 | 0.0118 | 0.0281 | 0 | 0.0123 | 0.0005 | 0.0107 | 0 | 0.0004 | 0 |
Mitochondria | 0.0256 | 0.0001 | 0.0096 | 0.0608 | 0.026 | 0.0001 | 0.0035 | 0.0002 | 0.004 | 0.0029 | 0.0003 | 0 | 0.0116 | 0.0001 | 0.0015 | 0.0001 | 0.0026 | 0 |
None | 0.0026 | 0.0081 | 0.0029 | 0.0011 | 0.0117 | 0.0052 | 0.0048 | 0.002 | 0.0002 | 0.0032 | 0.0007 | 0 | 0.005 | 0 | 0.0092 | 0 | 0.0007 | 0 |
Nuclear Periphery | 0.0339 | 0.0058 | 0.0022 | 0.0125 | 0.0041 | 0.0005 | 0.0067 | 0.0037 | 0.0013 | 0.0038 | 0.0036 | 0.0002 | 0.0035 | 0.0003 | 0.0027 | 0.0002 | 0.0019 | 0.0004 |
Nucleolus | 0.0269 | 0.0127 | 0.0142 | 0.0086 | 0.0568 | 0.0463 | 0.0201 | 0.0163 | 0.0108 | 0.0069 | 0.0574 | 0.0453 | 0.0193 | 0.0288 | 0.0128 | 0.0071 | 0.0239 | 0.0372 |
Nucleus | 0.7847 | 0.9568 | 0.9344 | 0.857 | 0.8273 | 0.9145 | 0.7597 | 0.96 | 0.9298 | 0.842 | 0.8533 | 0.9416 | 0.8715 | 0.9665 | 0.9089 | 0.9922 | 0.9047 | 0.9601 |
Peroxisomes | 0.0035 | 0 | 0.0038 | 0.002 | 0.0005 | 0.0017 | 0.0312 | 0 | 0.0154 | 0.0147 | 0.0042 | 0 | 0.0158 | 0.0001 | 0.0071 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.0242 | 0.0152 | 0.003 | 0.0019 | 0.0358 | 0.026 | 0.0267 | 0.0136 | 0.0091 | 0.0316 | 0.0412 | 0.0115 | 0.0119 | 0.0023 | 0.0088 | 0.0001 | 0.0015 | 0.0012 |
Vacuole | 0.004 | 0 | 0.0006 | 0.0026 | 0.005 | 0 | 0.0011 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0002 | 0 | 0.0003 | 0 |
Vacuole Periphery | 0.0029 | 0 | 0.0004 | 0.0024 | 0.0019 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0003 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5312 | 10.9964 | 5.7119 | 11.2724 | 12.7591 | 4.9177 | 10.5625 | 13.5076 | 10.1845 | 12.6219 |
Translational Efficiency | 0.7852 | 1.0932 | 1.4003 | 0.8523 | 0.9908 | 3.0442 | 1.1815 | 0.9761 | 1.3308 | 1.0199 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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