Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 4 | 8 | 3 | 1 | 7 | 2 | 1 | 6 | 14 | 11 |
Bud | 3 | 0 | 2 | 1 | 5 | 4 | – | 6 | 6 | 7 | 1 | 7 | 0 | 1 | 1 | 4 | 1 | 5 | 2 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 13 | 5 | 1 | 2 | 2 | – | 0 | 2 | 1 | 2 | 1 | 4 | 0 | 6 | 17 | 12 | 16 | 2 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 1 | 2 | 2 | 3 | – | 2 | 2 | 4 | 0 | 1 | 1 | 3 | 1 | 7 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 6 | 2 | 0 | 0 | 2 | 3 | – | 1 | 0 | 3 | 1 | 1 | 2 | 1 | 2 | 1 | 1 | 5 | 3 | 1 | 0 | 0 | 3 | 1 |
Endosome | 4 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 3 | 1 | 1 | 2 | 6 | 2 | 1 | 2 | 4 | 4 |
Golgi | 24 | 5 | 1 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 0 | 22 | 11 | 41 | 66 | 39 | 36 | 37 | 27 | 7 | 15 | 32 | 27 |
Mitochondria | 578 | 230 | 180 | 114 | 122 | 228 | – | 270 | 264 | 302 | 209 | 276 | 194 | 169 | 170 | 11 | 8 | 5 | 575 | 215 | 156 | 144 | 180 | 167 |
Nucleus | 3 | 1 | 1 | 0 | 0 | 2 | – | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 8 | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 29 | 9 | 12 | 1 | 5 | 31 | – | 24 | 45 | 38 | 27 | 29 | 7 | 7 | 4 | 2 | 5 | 3 | 5 | 0 | 1 | 1 | 0 | 0 |
Nucleolus | 30 | 9 | 9 | 5 | 4 | 31 | – | 46 | 33 | 42 | 25 | 26 | 9 | 5 | 5 | 8 | 9 | 7 | 1 | 1 | 1 | 0 | 1 | 2 |
Peroxisomes | 3 | 3 | 2 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 3 | 6 | 4 | 8 | 6 | 11 | 6 | 4 | 4 | 6 | 7 | 7 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 178 | 37 | 12 | 3 | 4 | 11 | – | 23 | 17 | 13 | 10 | 7 | 28 | 15 | 19 | 39 | 23 | 18 | 51 | 8 | 0 | 5 | 7 | 20 |
Unique Cell Count | 670 | 254 | 185 | 117 | 127 | 243 | 290 | 278 | 320 | 223 | 290 | 216 | 180 | 202 | 144 | 99 | 100 | 706 | 268 | 177 | 186 | 256 | 249 | |
Labelled Cell Count | 878 | 310 | 225 | 128 | 150 | 317 | 377 | 371 | 415 | 282 | 351 | 275 | 219 | 261 | 176 | 117 | 122 | 706 | 268 | 177 | 186 | 256 | 249 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.4 | 14.4 | 6.6 | 9.0 | 9.6 | 8.4 | – | 8.1 | 7.8 | 8.1 | 8.1 | 8.0 | 10.7 | 10.9 | 10.5 | 13.2 | 12.3 | 11.9 | 11.8 | 13.0 | 12.6 |
Std Deviation (1e-4) | 2.1 | 2.8 | 1.1 | 1.8 | 1.8 | 1.8 | – | 1.8 | 1.9 | 2.0 | 2.2 | 1.9 | 1.8 | 2.1 | 1.7 | 3.2 | 2.7 | 2.7 | 2.6 | 2.6 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.44 | 0.53 | 0.34 | – | 0.28 | 0.24 | 0.28 | 0.28 | 0.27 | 0.68 | 0.72 | 0.67 | 0.99 | 0.88 | 0.84 | 0.83 | 0.97 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 3.3 | 3.2 | 4.1 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.1 | 3.0 | 6.2 | 10.1 | 9.0 | 8.5 |
Mitochondria | 0.1 | -0.6 | -1.7 | – | -2.0 | -1.2 | -1.5 | -1.7 | -1.2 | -3.0 | -1.6 | -4.4 | -16.3 | -15.1 | -15.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.3 | -1.0 | 2.1 | – | 0.7 | 3.1 | 2.0 | 1.9 | 1.3 | -1.5 | -1.1 | -2.2 | -2.3 | -0.5 | -1.3 |
Nucleolus | -0.2 | -0.7 | 2.8 | – | 3.7 | 2.6 | 3.0 | 2.3 | 1.7 | -0.3 | -1.0 | -1.3 | 0.3 | 1.4 | 0.7 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | -1.3 | -0.9 | – | 0.6 | -0.2 | -1.2 | -0.9 | -2.2 | 2.2 | 0.7 | 1.1 | 5.1 | 4.1 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.6832 | 8.1748 | 8.5764 | 8.0992 | 6.925 | 8.3014 | 6.3629 | 6.9153 | 6.446 | 5.8439 | 6.4022 | 6.987 | 7.6842 | 8.7911 | 8.4293 | 8.5642 | 7.7642 | 8.7897 |
Actin | 0.0223 | 0.0056 | 0.0043 | 0.0005 | 0.0013 | 0.002 | 0.0172 | 0.0037 | 0.0028 | 0.0218 | 0.0152 | 0.002 | 0.04 | 0.0045 | 0.0062 | 0.0002 | 0.0084 | 0.0018 |
Bud | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.001 | 0.001 | 0.0003 | 0.0009 | 0.0006 | 0.0001 | 0.0005 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.001 | 0.0001 | 0.0005 | 0.007 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0 | 0.0003 | 0 |
Bud Periphery | 0.0008 | 0.0012 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0023 | 0.0023 | 0.0009 | 0.0041 | 0.0011 | 0.0001 | 0.0011 | 0.0007 | 0.0004 | 0.0003 | 0.0002 | 0.0002 |
Bud Site | 0.0012 | 0.0007 | 0.0029 | 0.0002 | 0.0001 | 0 | 0.0065 | 0.0036 | 0.0008 | 0.0062 | 0.001 | 0.0001 | 0.0009 | 0.0011 | 0.0027 | 0.0001 | 0.0016 | 0 |
Cell Periphery | 0.0012 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0027 | 0.0016 | 0.005 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0015 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0023 | 0.0004 | 0.0001 | 0.0003 | 0.0008 | 0.0006 | 0.0055 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0024 |
Cytoplasmic Foci | 0.0068 | 0.0023 | 0.0029 | 0.0028 | 0.0031 | 0.0007 | 0.0116 | 0.0062 | 0.007 | 0.0024 | 0.004 | 0.001 | 0.0272 | 0.0028 | 0.0046 | 0.0076 | 0.008 | 0.0014 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.001 | 0.0008 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0026 | 0.0002 | 0.0003 | 0.0004 | 0.003 | 0.0003 | 0.0109 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.0007 |
Endosome | 0.0128 | 0.0151 | 0.0083 | 0.0009 | 0.0069 | 0.0036 | 0.031 | 0.0091 | 0.0095 | 0.0052 | 0.0281 | 0.0063 | 0.0551 | 0.0121 | 0.0121 | 0.0016 | 0.0057 | 0.0071 |
Golgi | 0.0076 | 0.0136 | 0.0106 | 0.0017 | 0.0073 | 0.0136 | 0.017 | 0.0163 | 0.0121 | 0.0084 | 0.0216 | 0.012 | 0.0287 | 0.0324 | 0.0132 | 0.0036 | 0.0064 | 0.0202 |
Lipid Particles | 0.0075 | 0.0067 | 0.0029 | 0.0197 | 0.0174 | 0.0016 | 0.0169 | 0.0015 | 0.0062 | 0.0066 | 0.0085 | 0.0013 | 0.0374 | 0.0064 | 0.0065 | 0.0279 | 0.0704 | 0.0086 |
Mitochondria | 0.8864 | 0.9279 | 0.9398 | 0.9563 | 0.9491 | 0.9662 | 0.8343 | 0.9344 | 0.9379 | 0.9121 | 0.8882 | 0.9637 | 0.7271 | 0.9167 | 0.9279 | 0.9487 | 0.882 | 0.9479 |
None | 0.0054 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.002 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0047 | 0.0058 | 0 | 0.0004 | 0 | 0.0002 | 0.0007 |
Nuclear Periphery | 0.0047 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0023 | 0.0066 | 0.0001 | 0.001 | 0.0003 | 0.015 | 0.0011 | 0.0077 | 0.0001 | 0.0026 | 0 | 0.0002 | 0.0008 |
Nucleolus | 0.0027 | 0.0003 | 0.0001 | 0.0011 | 0.0002 | 0.0003 | 0.0055 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0011 | 0.0001 | 0.0001 |
Nucleus | 0.004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0054 | 0.0002 | 0.0002 | 0.0005 | 0.0017 | 0.0007 | 0.001 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0.0003 |
Peroxisomes | 0.0044 | 0.005 | 0.0028 | 0.0142 | 0.0058 | 0.0011 | 0.0163 | 0.0066 | 0.0097 | 0.0109 | 0.0017 | 0.0015 | 0.0282 | 0.0092 | 0.0118 | 0.007 | 0.0143 | 0.0029 |
Punctate Nuclear | 0.0062 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.003 | 0.004 | 0 | 0.0001 | 0.0001 | 0.004 | 0.0009 | 0.005 | 0 | 0.0027 | 0.0008 | 0.0006 | 0.0003 |
Vacuole | 0.0078 | 0.0084 | 0.0073 | 0.0007 | 0.0016 | 0.0004 | 0.0082 | 0.0047 | 0.0036 | 0.0032 | 0.0015 | 0.0013 | 0.006 | 0.0051 | 0.0015 | 0.0002 | 0.0003 | 0.001 |
Vacuole Periphery | 0.0141 | 0.0116 | 0.0155 | 0.0011 | 0.0059 | 0.0022 | 0.0074 | 0.0065 | 0.0041 | 0.0031 | 0.0024 | 0.002 | 0.0099 | 0.007 | 0.0038 | 0.0004 | 0.0004 | 0.0033 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.4105 | 4.0343 | 13.4712 | 12.7715 | 24.8675 | 2.2552 | 5.6087 | 9.6358 | 15.0894 | 15.5953 |
Translational Efficiency | 2.761 | 8.0475 | 1.7523 | 2.0878 | 1.3412 | 18.5358 | 7.2385 | 4.2073 | 2.4389 | 2.7003 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1956 | 417 | 181 | 1388 | 1876 | 2143 | 2332 | 1462 | 3832 | 2560 | 2513 | 2850 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 952.28 | 919.34 | 1556.93 | 1295.25 | 898.34 | 1068.54 | 1324.91 | 1312.61 | 925.87 | 1044.24 | 1341.62 | 1304.16 |
Standard Deviation | 150.73 | 167.08 | 281.37 | 224.75 | 154.77 | 176.19 | 213.59 | 218.93 | 155.08 | 183.22 | 227.23 | 221.96 |
Intensity Change Log 2 | — | -0.050787 | 0.709246 | 0.443773 | — | 0.250307 | 0.560561 | 0.547105 | — | 0.103222 | 0.638982 | 0.494858 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000553 | 0.005146 | 0.007320 | 0.002904 | 0.000868 | 0.001088 | 0.001123 | 0.002841 | 0.000707 | 0.001749 | 0.001569 | 0.002872 |
Bud Neck | 0.000943 | 0.002698 | 0.010270 | 0.001803 | 0.000735 | 0.000501 | 0.001182 | 0.001124 | 0.000841 | 0.000859 | 0.001837 | 0.001454 |
Bud Site | 0.001990 | 0.006100 | 0.005495 | 0.007614 | 0.002331 | 0.001220 | 0.002730 | 0.005416 | 0.002157 | 0.002015 | 0.002929 | 0.006487 |
Cell Periphery | 0.000312 | 0.002464 | 0.000338 | 0.000552 | 0.000988 | 0.000221 | 0.000144 | 0.000500 | 0.000643 | 0.000586 | 0.000158 | 0.000526 |
Cytoplasm | 0.000346 | 0.001452 | 0.000527 | 0.000816 | 0.000169 | 0.000239 | 0.000456 | 0.000618 | 0.000260 | 0.000436 | 0.000461 | 0.000714 |
Cytoplasmic Foci | 0.006069 | 0.010215 | 0.046683 | 0.008422 | 0.003249 | 0.007556 | 0.006066 | 0.007639 | 0.004689 | 0.007989 | 0.008992 | 0.008020 |
Eisosomes | 0.000053 | 0.000190 | 0.000240 | 0.000096 | 0.000069 | 0.000051 | 0.000053 | 0.000096 | 0.000061 | 0.000074 | 0.000067 | 0.000096 |
Endoplasmic Reticulum | 0.000787 | 0.002680 | 0.000540 | 0.001048 | 0.000154 | 0.000138 | 0.000102 | 0.000575 | 0.000477 | 0.000552 | 0.000133 | 0.000805 |
Endosome | 0.009965 | 0.018107 | 0.018900 | 0.013203 | 0.004390 | 0.009984 | 0.005576 | 0.010917 | 0.007236 | 0.011307 | 0.006536 | 0.012031 |
Golgi | 0.010304 | 0.022996 | 0.042168 | 0.045731 | 0.005692 | 0.017161 | 0.013786 | 0.048775 | 0.008046 | 0.018112 | 0.015830 | 0.047292 |
Lipid Particles | 0.003855 | 0.002010 | 0.005362 | 0.003195 | 0.001752 | 0.002254 | 0.000869 | 0.001435 | 0.002826 | 0.002214 | 0.001193 | 0.002292 |
Mitochondria | 0.944355* | 0.895030* | 0.757255* | 0.837169* | 0.963549* | 0.940471* | 0.931364* | 0.855120* | 0.953752* | 0.933069* | 0.918823* | 0.846377* |
Mitotic Spindle | 0.000572 | 0.000995 | 0.021606 | 0.006201 | 0.000184 | 0.001046 | 0.004449 | 0.005352 | 0.000382 | 0.001038 | 0.005685 | 0.005766 |
None | 0.000653 | 0.001219 | 0.001844 | 0.001079 | 0.000556 | 0.000403 | 0.000832 | 0.000999 | 0.000605 | 0.000536 | 0.000905 | 0.001038 |
Nuclear Periphery | 0.000159 | 0.000067 | 0.004389 | 0.000948 | 0.000083 | 0.000109 | 0.000505 | 0.000376 | 0.000122 | 0.000102 | 0.000784 | 0.000655 |
Nuclear Periphery Foci | 0.000182 | 0.000129 | 0.002007 | 0.001231 | 0.000103 | 0.000150 | 0.000251 | 0.000335 | 0.000144 | 0.000147 | 0.000377 | 0.000771 |
Nucleolus | 0.000128 | 0.001071 | 0.001070 | 0.000511 | 0.000121 | 0.000110 | 0.000324 | 0.000140 | 0.000124 | 0.000267 | 0.000378 | 0.000321 |
Nucleus | 0.000060 | 0.000679 | 0.000424 | 0.000249 | 0.000055 | 0.000072 | 0.000095 | 0.000061 | 0.000058 | 0.000171 | 0.000119 | 0.000152 |
Peroxisomes | 0.005388 | 0.016491 | 0.019315 | 0.003928 | 0.005976 | 0.004317 | 0.003872 | 0.002214 | 0.005676 | 0.006300 | 0.004985 | 0.003049 |
Vacuole | 0.005286 | 0.006252 | 0.025656 | 0.025555 | 0.003780 | 0.006380 | 0.009876 | 0.022980 | 0.004548 | 0.006359 | 0.011013 | 0.024234 |
Vacuole Periphery | 0.008040 | 0.004008 | 0.028590 | 0.037744 | 0.005194 | 0.006530 | 0.016344 | 0.032488 | 0.006647 | 0.006119 | 0.017226 | 0.035048 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.90 | -1.92 | -3.71 | 1.31 | 1.23 | -0.62 | -0.69 | -2.50 | -2.14 | -2.06 | -2.79 | -2.13 | -4.26 | -1.85 | -2.05 |
Bud Neck | -2.17 | -4.38 | -1.89 | 1.16 | 4.01 | 1.25 | -1.88 | -1.84 | -3.36 | 0.24 | -0.08 | -3.14 | -2.43 | -2.96 | 1.35 |
Bud Site | -2.17 | -2.27 | -4.74 | -0.74 | -1.19 | 1.51 | -0.54 | -2.50 | -3.83 | -2.31 | 0.24 | -1.32 | -5.07 | -5.25 | -4.03 |
Cell Periphery | -2.03 | -0.27 | -2.83 | 1.80 | -2.22 | 1.91 | 2.15 | 1.23 | -2.71 | -5.94 | 0.21 | 2.49 | 0.59 | 0.31 | -8.25 |
Cytoplasm | -1.87 | -1.23 | -2.62 | 1.05 | -1.73 | -1.13 | -3.26 | -3.46 | -2.76 | -1.00 | -1.46 | -2.11 | -4.09 | -1.99 | -1.99 |
Cytoplasmic Foci | -2.18 | -5.98 | -2.04 | 0.88 | 5.60 | -4.85 | -3.59 | -4.66 | -0.08 | -1.58 | -4.16 | -4.93 | -4.47 | -0.05 | 1.01 |
Eisosomes | -4.58 | -5.75 | -4.94 | 3.05 | 4.32 | 2.34 | 2.09 | -1.90 | -3.24 | -3.10 | -1.74 | -1.00 | -4.19 | -2.25 | -3.20 |
Endoplasmic Reticulum | -1.15 | 0.48 | -0.47 | 1.03 | -1.76 | 0.43 | 2.43 | -4.02 | -4.06 | -4.57 | -0.21 | 1.35 | -1.15 | -0.88 | -4.98 |
Endosome | -2.46 | -3.11 | -1.98 | 1.45 | 1.93 | -5.23 | -1.44 | -5.29 | -0.69 | -4.44 | -3.73 | 0.82 | -4.66 | -0.62 | -5.58 |
Golgi | -3.13 | -4.83 | -9.57 | -4.29 | -0.48 | -6.97 | -5.53 | -12.06 | -8.41 | -9.50 | -6.67 | -5.58 | -15.25 | -10.30 | -11.34 |
Lipid Particles | 2.22 | -1.00 | 0.58 | -1.32 | 1.38 | -0.82 | 2.24 | 0.58 | 1.30 | -1.43 | 1.01 | 3.67 | 0.84 | -0.14 | -2.35 |
Mitochondria | 4.10 | 8.45 | 12.78 | 4.24 | -3.44 | 5.00 | 7.03 | 14.54 | 11.07 | 9.89 | 4.88 | 7.94 | 19.14 | 14.01 | 11.47 |
Mitotic Spindle | -1.59 | -3.45 | -5.92 | -5.35 | 2.49 | -3.40 | -6.09 | -5.28 | -4.26 | -0.74 | -2.95 | -6.70 | -7.88 | -6.63 | -0.08 |
None | -0.90 | -4.81 | -6.57 | 0.22 | 3.07 | 4.07 | -5.90 | -8.96 | -12.41 | -3.01 | 0.64 | -6.35 | -10.59 | -4.60 | -2.65 |
Nuclear Periphery | 3.56 | -2.35 | -3.47 | -3.88 | 1.90 | -0.95 | -3.08 | -4.43 | -3.96 | 0.85 | 0.84 | -3.64 | -4.59 | -4.73 | 0.60 |
Nuclear Periphery Foci | 1.30 | -3.90 | -2.83 | -2.98 | 1.29 | -0.97 | -3.27 | -3.13 | -2.17 | -1.02 | -0.10 | -4.29 | -3.40 | -3.32 | -2.07 |
Nucleolus | -1.05 | -3.43 | -2.06 | 0.61 | 1.71 | 0.22 | -2.16 | -0.47 | -0.80 | 2.02 | -0.95 | -2.88 | -2.11 | -0.32 | 0.46 |
Nucleus | -1.75 | -2.24 | -1.71 | 1.16 | 0.89 | -0.66 | -1.86 | -0.39 | 0.47 | 1.76 | -1.84 | -2.72 | -1.74 | 0.22 | -0.56 |
Peroxisomes | -2.85 | -2.76 | 1.23 | 3.21 | 3.05 | 1.24 | 1.68 | 3.32 | 2.50 | 2.48 | -0.56 | 0.71 | 3.23 | 3.23 | 2.45 |
Vacuole | -0.49 | -4.38 | -9.24 | -6.86 | 0.00 | -2.73 | -6.35 | -8.73 | -7.30 | -5.75 | -2.18 | -7.11 | -12.66 | -10.72 | -7.74 |
Vacuole Periphery | 3.67 | -4.91 | -11.08 | -12.47 | -1.89 | -1.55 | -8.49 | -11.80 | -11.29 | -6.45 | 0.75 | -8.73 | -16.08 | -16.33 | -8.79 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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