Standard name
Human Ortholog
Description Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0.07 – 0.1 0.13 0.08 0.14 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.99 0.96 0.94 0.85 – 0.78 0.76 0.79 0.76 1.0 0.99 0.99 – – – 0.99 0.96 0.94 0.96 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.12 – 0.16 0.15 0.13 0.14 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 4 – 11 7 10 15 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 – 1 1 0 1 0 0 1 – – – 0 1 0 0 3 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 2 3 3 2 6 9 25 – 28 38 21 52 1 14 20 – – – 0 0 0 0 0 0
Cytoplasm 115 119 165 184 260 259 322 – 224 225 201 290 222 497 530 – – – 112 119 155 124 337 337
Endoplasmic Reticulum 1 1 3 2 11 14 46 – 47 45 34 54 1 1 2 – – – 0 0 3 0 3 3
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 5 – 4 7 2 12 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 – 0 0 5 1 0 1 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 – – – 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 – 0 1 0 1 0 0 4 – – – 0 0 1 0 3 3
Unique Cell Count 116 120 168 185 271 276 381 287 297 253 383 223 500 535 114 124 165 129 352 352
Labelled Cell Count 118 123 171 188 277 284 404 315 324 273 426 224 513 558 114 124 165 129 352 352


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 79.8 99.6 71.9 67.1 63.2 56.2 50.4 – 44.5 45.5 41.8 42.6 78.7 79.1 72.9 – – – 66.3 69.4 69.4
Std Deviation (1e-4) 12.5 22.2 16.9 14.1 15.8 10.7 11.8 – 10.2 9.6 9.3 9.9 17.3 17.8 18.5 – – – 19.3 19.3 19.3
Intensity Change (Log2) – – – -0.1 -0.19 -0.35 -0.51 – -0.69 -0.66 -0.78 -0.76 0.13 0.14 0.02 – – – -0.12 -0.05 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 2.3 – 3.3 4.0 2.8 4.3 0 0 1.2 – – –
Cytoplasm 1.1 -1.3 -2.2 -4.7 – -5.9 -6.3 -5.6 -6.4 1.3 1.4 0.9 – – –
Endoplasmic Reticulum 0 1.3 1.8 3.9 – 4.8 4.6 4.1 4.4 0 0 0 – – –
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 50.8806 71.6965 63.8347 64.4053 52.4574 69.3058 59.6847 82.8995 74.7699 68.6584 59.7285 76.6267 62.7278 89.0091 79.9171 69.2479 76.8604 79.7187
Actin 0.0038 0.0002 0.0125 0.0001 0.1503 0.0005 0.0078 0.0002 0.0016 0.0011 0.0017 0.0007 0.0014 0.0001 0.0099 0.011 0.0018 0.0026
Bud 0.0012 0.0017 0.0025 0.0006 0.0015 0.0011 0.0009 0.0001 0.0009 0.0041 0.0006 0.0006 0.0011 0.0001 0.0009 0.002 0.0003 0.0008
Bud Neck 0.0013 0.0003 0.0007 0.0009 0.0021 0.0027 0.0004 0.0001 0.0004 0.0006 0.0005 0.0008 0.0006 0.0002 0.0004 0.0007 0.0002 0.0006
Bud Periphery 0.0028 0.001 0.0024 0.0002 0.002 0.0019 0.0009 0 0.0019 0.0008 0.0004 0.0002 0.0009 0 0.0006 0.0031 0.0006 0.0009
Bud Site 0.0016 0.001 0.0028 0.0004 0.005 0.0001 0.0033 0.0002 0.0106 0.001 0.0006 0 0.0007 0.0001 0.0073 0.0055 0.0001 0.0001
Cell Periphery 0.0055 0.003 0.0011 0.0007 0.0002 0.0029 0.0012 0.0019 0.0015 0.0002 0.0004 0.0002 0.0075 0.0011 0.0013 0.0014 0.0001 0.0004
Cytoplasm 0.7913 0.9695 0.9125 0.8593 0.6531 0.9421 0.867 0.9719 0.9329 0.9236 0.8027 0.9662 0.8493 0.9888 0.9177 0.8375 0.909 0.9334
Cytoplasmic Foci 0.012 0.0005 0.0053 0.014 0.0097 0.0022 0.0087 0.0055 0.0017 0.0064 0.0823 0.0038 0.0078 0.0004 0.0046 0.0084 0.0012 0.0194
Eisosomes 0.0002 0 0.0003 0 0.0005 0 0.0001 0 0 0 0 0 0.0002 0 0.0002 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0293 0.0018 0.0101 0.0011 0.0264 0.0041 0.022 0.0064 0.0063 0.0045 0.0057 0.0036 0.0196 0.003 0.0069 0.0037 0.0029 0.0144
Endosome 0.0129 0.0007 0.0054 0.0322 0.0119 0.0056 0.0103 0.0014 0.0049 0.0228 0.0236 0.0061 0.0109 0.0003 0.004 0.0168 0.0128 0.003
Golgi 0.0045 0.0001 0.0017 0.0039 0.0152 0.0009 0.0023 0.0005 0.0006 0.0074 0.0016 0.0016 0.0012 0 0.0031 0.0059 0.0059 0.0021
Lipid Particles 0.0168 0.0001 0.0031 0.0021 0.0061 0.0003 0.0027 0.0006 0.0003 0.0004 0.0061 0.0001 0.0067 0.0003 0.0019 0.0037 0.0004 0.0089
Mitochondria 0.0143 0.001 0.0046 0.0006 0.0321 0.001 0.0033 0.0008 0.0121 0.0033 0.0022 0.0006 0.0057 0 0.003 0.0301 0.0469 0.0017
None 0.0026 0.0002 0.0014 0.0002 0.0002 0.0002 0.0019 0.0013 0.0006 0.0011 0.0132 0.0002 0.0041 0.0001 0.0045 0.0043 0.0002 0.0038
Nuclear Periphery 0.0247 0.001 0.0029 0.0045 0.0337 0.0012 0.0131 0.001 0.0021 0.0022 0.0071 0.0011 0.015 0.0011 0.0034 0.0036 0.0018 0.0015
Nucleolus 0.0031 0.0004 0.0014 0.0001 0.0001 0.0001 0.0009 0.0004 0.0004 0.0002 0.001 0 0.0063 0 0.0019 0.0037 0 0.0001
Nucleus 0.006 0.0017 0.0027 0.0023 0.0018 0.0013 0.006 0.001 0.0018 0.0016 0.0038 0.001 0.0074 0.0008 0.0027 0.0053 0.0007 0.0008
Peroxisomes 0.0065 0.0001 0.0012 0.0011 0.022 0.0001 0.0008 0.0003 0.0001 0.0004 0.001 0 0.0006 0 0.0006 0.0017 0 0.0005
Punctate Nuclear 0.0008 0 0.0002 0.0001 0.0003 0 0.0025 0.0003 0.0001 0.0001 0.0186 0 0.0015 0 0.0004 0.0007 0 0.0007
Vacuole 0.0444 0.014 0.0204 0.0643 0.0193 0.0264 0.0351 0.0055 0.0153 0.0154 0.0215 0.0105 0.0424 0.0034 0.0193 0.0348 0.0101 0.0037
Vacuole Periphery 0.0145 0.0018 0.0048 0.0113 0.0065 0.0051 0.009 0.0005 0.0038 0.0026 0.0054 0.0025 0.009 0.0002 0.0056 0.0158 0.0048 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 367.2509 399.6694 392.2639 351.881 366.2196 407.2192 364.7896 344.7926 346.3695 320.3604
Translational Efficiency 1.2058 1.2401 1.1555 1.5095 1.075 0.9418 1.2042 1.018 0.9591 1.0038

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2123 1665 1057 1479 1723 1802 216 92 3846 3467 1273 1571

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3965.67 4245.47 6184.20 6564.24 4193.04 4735.29 7288.81 6720.89 4067.53 4500.06 6371.63 6573.41
Standard Deviation 750.44 1234.03 1227.16 1468.40 857.69 972.69 1131.65 1130.47 808.21 1132.68 1280.47 1451.24
Intensity Change Log 2 — 0.098360 0.641022 0.727063 — 0.175456 0.797687 0.680656 — 0.138496 0.723659 0.703399

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000030 0.000058 0.000106 0.000265 0.000036 0.000072 0.000163 0.000188 0.000033 0.000065 0.000115 0.000260
Bud Neck 0.000125 0.000207 0.001525 0.001910 0.000202 0.000397 0.004320 0.002521 0.000160 0.000306 0.001999 0.001945
Bud Site 0.001588 0.001137 0.002727 0.011622 0.000787 0.002945 0.002578 0.007005 0.001229 0.002076 0.002702 0.011352
Cell Periphery 0.002806 0.003722 0.016035 0.021671 0.006720 0.003218 0.022590 0.048059 0.004559 0.003460 0.017147 0.023216
Cytoplasm 0.921375* 0.916784* 0.771433* 0.760971* 0.872538* 0.900595* 0.652885* 0.539990* 0.899496* 0.908370* 0.751318* 0.748030*
Cytoplasmic Foci 0.004945 0.002343 0.007960 0.007507 0.004435 0.005652 0.016152 0.023835 0.004717 0.004063 0.009350 0.008464
Eisosomes 0.000000 0.000001 0.000003 0.000005 0.000001 0.000001 0.000007 0.000005 0.000000 0.000001 0.000003 0.000005
Endoplasmic Reticulum 0.000591 0.000137 0.000160 0.000197 0.000361 0.000193 0.000115 0.000061 0.000488 0.000166 0.000153 0.000189
Endosome 0.000320 0.000162 0.000294 0.000895 0.000271 0.000448 0.000418 0.000454 0.000298 0.000311 0.000315 0.000869
Golgi 0.021249 0.011624 0.015176 0.007598 0.032885 0.017958 0.018093 0.012900 0.026462 0.014916 0.015671 0.007908
Lipid Particles 0.002885 0.003454 0.009544 0.014101 0.005291 0.003827 0.022029 0.036471 0.003963 0.003648 0.011662 0.015411
Mitochondria 0.000093 0.000099 0.000128 0.000329 0.000085 0.000144 0.000110 0.000054 0.000089 0.000122 0.000125 0.000313
Mitotic Spindle 0.000559 0.000212 0.000980 0.000708 0.000377 0.000950 0.001041 0.000570 0.000478 0.000596 0.000990 0.000700
None 0.001842 0.007847 0.001864 0.001944 0.002264 0.002333 0.001660 0.001143 0.002031 0.004981 0.001829 0.001897
Nuclear Periphery 0.000119 0.000148 0.001018 0.000421 0.000363 0.000168 0.001558 0.000595 0.000228 0.000158 0.001110 0.000431
Nuclear Periphery Foci 0.004939 0.003881 0.008507 0.003514 0.009907 0.004730 0.011185 0.007222 0.007164 0.004322 0.008961 0.003731
Nucleolus 0.000021 0.000073 0.000081 0.000210 0.000074 0.000055 0.000101 0.000097 0.000044 0.000064 0.000084 0.000203
Nucleus 0.004991 0.017124 0.035154 0.015166 0.007913 0.012088 0.036600 0.030678 0.006300 0.014507 0.035399 0.016075
Peroxisomes 0.000012 0.000012 0.000009 0.000110 0.000010 0.000015 0.000013 0.000049 0.000011 0.000014 0.000010 0.000107
Vacuole 0.011927 0.021321 0.108900 0.141047 0.029448 0.030631 0.189719 0.279902* 0.019776 0.026160 0.122613 0.149178
Vacuole Periphery 0.019584 0.009653 0.018398 0.009810 0.026035 0.013582 0.018663 0.008202 0.022474 0.011695 0.018443 0.009716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.66 -8.51 -6.95 -5.74 -4.31 -2.95 -5.77 -4.61 -3.30 0.61 -3.23 -10.41 -7.17 -5.95 -3.91
Bud Neck -3.20 -10.89 -10.90 -10.43 -1.26 -3.94 -7.26 -4.74 -4.35 2.68 -5.13 -12.45 -11.45 -10.45 1.11
Bud Site 1.88 -3.07 -7.37 -7.69 -6.13 -5.17 -5.34 -3.08 -2.00 -2.06 -3.86 -4.79 -7.92 -6.97 -6.35
Cell Periphery -1.76 -11.21 -12.90 -11.81 -2.14 5.33 -6.38 -4.93 -5.42 -2.47 2.64 -11.71 -12.55 -13.41 -2.28
Cytoplasm 1.14 17.95 21.89 20.63 0.82 -3.44 11.40 9.88 10.59 1.83 -1.34 19.37 21.39 22.24 -0.24
Cytoplasmic Foci 6.32 -5.45 -3.57 -5.56 0.30 -2.52 -5.91 -2.36 -2.23 -0.71 1.40 -7.93 -4.40 -4.77 0.80
Eisosomes -3.11 -9.79 -8.95 -8.32 -3.16 -1.62 -7.15 -8.82 -8.42 2.92 -3.41 -11.72 -9.36 -8.82 -1.51
Endoplasmic Reticulum 2.35 2.22 2.05 -1.36 -0.74 2.76 4.30 5.34 6.55 4.41 2.91 3.03 2.67 -0.44 -0.81
Endosome 3.76 -0.42 -2.07 -2.64 -2.00 -2.43 -3.93 -2.01 -0.52 -0.46 -0.54 -1.86 -2.21 -2.09 -1.95
Golgi 5.79 3.72 9.30 3.59 7.83 7.12 6.78 6.46 1.95 1.69 8.70 8.34 15.03 7.14 8.51
Lipid Particles -0.99 -9.62 -11.73 -10.36 -3.64 2.01 -6.44 -5.43 -5.65 -2.06 0.46 -10.66 -12.02 -11.98 -2.68
Mitochondria -0.30 -2.00 -2.86 -2.87 -2.19 -1.92 -1.73 1.79 2.96 3.28 -1.59 -2.78 -2.94 -2.51 -2.20
Mitotic Spindle 3.82 -1.83 -1.22 -2.44 0.80 -3.18 -3.85 -1.08 -0.35 -0.08 -1.39 -2.43 -1.68 -1.05 0.83
None -4.81 -0.25 -0.32 4.35 -0.24 -0.15 2.81 3.57 2.47 1.42 -4.59 1.54 0.16 3.81 -0.26
Nuclear Periphery -1.94 -11.57 -1.93 -1.75 3.39 3.34 -7.10 -1.82 -2.57 3.12 2.32 -12.29 -1.54 -1.89 4.06
Nuclear Periphery Foci 1.91 -5.22 2.08 0.24 6.61 6.02 -0.81 1.86 -2.00 2.17 5.81 -3.04 5.84 0.91 7.50
Nucleolus -5.46 -14.78 -4.03 -2.98 -2.64 0.59 -0.55 -0.52 -2.57 -0.01 -0.63 -2.01 -3.21 -3.20 -2.58
Nucleus -10.94 -15.98 -12.37 -1.76 8.14 -4.95 -7.52 -3.77 -3.05 1.60 -11.72 -17.18 -11.85 -4.28 8.84
Peroxisomes -0.15 0.72 -5.79 -5.73 -5.93 -1.00 -1.26 -2.93 -2.69 -2.65 -0.95 0.12 -5.97 -5.81 -6.03
Vacuole -4.82 -17.69 -25.48 -22.79 -4.21 -1.04 -10.78 -8.74 -8.64 -1.99 -4.40 -19.52 -25.40 -23.69 -3.19
Vacuole Periphery 5.73 0.57 6.52 0.28 6.67 5.98 2.84 8.29 3.49 3.41 8.03 2.67 10.34 2.60 7.23
Standard name
Human Ortholog
Description Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Grs1

Grs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Grs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available