Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.07 0 0.05 – 0 0.05 0 0 0.1 0.2 0.2 0.1 0.1 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.12 0.06 – 0.25 0.19 0.38 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.91 0.93 0.9 0.88 0.83 0.78 – 0.76 0.74 0.66 0.71 0.81 0.74 0.74 0.8 0.79 0.81 0.89 0.88 0.82 0.79 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0.08 0.07 0.17 0.18 – 0.33 0.22 0.36 0.35 0.08 0.07 0 0 0 0 0 0 0 0.08 0.07 0.12
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0.06 0.05 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 – 0 1 4 1 0 0 0 0 1 0 1 4 1 0 0 2
Bud Neck 0 0 0 0 1 1 0 – 1 2 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 2 2 – 7 8 20 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 11 9 10 13 7 14 – 4 14 8 3 5 40 45 42 32 29 0 3 1 0 2 5
Endoplasmic Reticulum 0 1 0 1 0 0 0 – 0 0 0 1 0 2 0 15 11 7 0 0 2 0 1 0
Endosome 0 3 1 1 1 1 4 – 0 2 0 0 1 3 4 10 5 5 2 6 2 1 6 6
Golgi 0 1 0 0 0 1 0 – 0 0 1 0 0 0 1 0 3 1 0 2 0 1 1 1
Mitochondria 7 11 0 5 3 26 18 – 52 51 117 42 1 0 0 17 12 8 1 2 3 1 6 8
Nucleus 91 313 165 177 175 186 226 – 158 201 206 169 42 148 165 343 264 271 98 314 162 106 166 160
Nuclear Periphery 0 0 0 0 0 3 1 – 0 0 1 1 1 0 0 3 2 1 0 0 0 0 0 1
Nucleolus 5 7 0 16 15 38 51 – 69 59 111 83 4 14 8 7 8 7 3 6 5 10 15 30
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 6 9 4 10 – 2 9 3 3 4 4 2 5 2 1 0 3 1 3 6 2
Vac/Vac Membrane 0 8 4 3 3 1 3 – 1 5 1 1 0 4 5 29 25 22 2 13 11 6 22 36
Unique Cell Count 98 344 178 197 200 225 289 207 273 311 237 52 200 223 427 333 333 111 359 199 136 237 265
Labelled Cell Count 106 356 181 220 221 270 330 294 352 475 317 59 216 231 471 365 352 111 359 199 136 237 265


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.4 6.9 6.7 7.2 6.1 6.4 – 5.1 5.6 5.7 5.2 7.5 7.4 7.1 8.6 8.6 8.3 6.1 6.8 6.9
Std Deviation (1e-4) 0.9 1.7 1.2 1.7 1.6 1.4 1.5 – 1.1 1.3 6.6 1.1 2.0 1.5 1.4 1.7 1.8 1.8 0.8 1.2 1.3
Intensity Change (Log2) – – – -0.05 0.06 -0.18 -0.11 – -0.43 -0.31 -0.29 -0.4 0.11 0.1 0.03 0.31 0.31 0.26 -0.19 -0.03 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 3.5 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.6 -1.0 -0.1 – -1.7 0 -1.4 -2.3 0 4.3 4.4 1.9 1.8 1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.7 3.4 – 7.2 6.1 9.4 5.9 0 0 0 2.7 0 0
Nucleus -1.0 -1.7 -3.0 -4.1 – -4.4 -5.1 -6.6 -5.4 -2.5 -4.8 -4.9 -3.8 -3.9 -3.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.9 3.7 5.8 5.9 – 8.5 6.7 9.1 8.8 0 3.6 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1.9 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 2.2 2.4 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9695 0.9988 0.8605 0.5767 0.4393 0.7799 2.2872 1.8068 1.8009 2.4349 1.9844 1.8293 1.4954 1.2415 0.9691 1.5475 1.4164 1.2319
Actin 0.0298 0 0.0149 0.009 0 0.001 0.048 0.0073 0.0107 0.0223 0.04 0.0031 0.0393 0 0.0172 0 0 0.0024
Bud 0.0008 0 0.0003 0.0009 0 0 0.0004 0 0.0019 0.0026 0.0011 0.0001 0.0011 0.0004 0.0002 0 0 0.0001
Bud Neck 0.0009 0.0001 0.0005 0.0004 0.0017 0.0018 0.0023 0.0001 0.001 0.0049 0.0031 0.0013 0.0078 0.0001 0.0003 0.0001 0.0006 0.0011
Bud Periphery 0.0012 0 0.0003 0.0007 0.0001 0.0001 0.0006 0 0.0034 0.0082 0.0017 0.0002 0.0016 0.0001 0.0001 0 0 0.0005
Bud Site 0.0029 0 0.0006 0.0046 0.0001 0.0002 0.0025 0.0002 0.0028 0.0037 0.0078 0.0001 0.0076 0.0002 0.0011 0 0 0.0001
Cell Periphery 0.0003 0 0 0.0001 0 0 0.0003 0 0.0004 0.0006 0.0003 0 0.0005 0 0 0 0 0.0001
Cytoplasm 0.0237 0.0002 0.0007 0.0098 0.0001 0.0001 0.0081 0.0002 0.0048 0.0009 0.0044 0.0001 0.0183 0.0018 0.0015 0 0.0002 0.001
Cytoplasmic Foci 0.0312 0 0.0047 0.0598 0 0.0032 0.0276 0.0001 0.0116 0.0075 0.0159 0.0014 0.014 0.0002 0.012 0 0 0.0043
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0003 0 0.0002 0.0003 0.0002 0 0.0006 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0052 0 0.0001 0.0025 0 0 0.0053 0 0.0018 0.0015 0.0018 0.0001 0.0081 0.0001 0.0016 0 0 0.0016
Endosome 0.0252 0 0.0038 0.0639 0.0001 0.0021 0.0209 0.0001 0.0064 0.0269 0.0228 0.0018 0.043 0 0.0031 0 0 0.0216
Golgi 0.0087 0 0.0027 0.0312 0 0.0026 0.0081 0.0002 0.0094 0.0171 0.0051 0.0035 0.0114 0 0.0028 0 0 0.0068
Lipid Particles 0.0301 0 0.0049 0.05 0 0.0057 0.0174 0 0.0076 0.0229 0.0201 0.0036 0.0171 0 0.0013 0 0 0.0036
Mitochondria 0.0105 0.0001 0.0007 0.0147 0.0003 0.0003 0.0025 0.0004 0.002 0.0401 0.0088 0.0056 0.0093 0.0007 0.016 0.0001 0.0001 0.0138
None 0.0085 0 0.0034 0.004 0.0001 0 0.003 0 0.0005 0.0007 0.017 0 0.0131 0.0002 0.0003 0 0 0.0004
Nuclear Periphery 0.0285 0.0024 0.0008 0.0112 0.0003 0.0006 0.0069 0.0009 0.0078 0.0038 0.0342 0.0004 0.0101 0.0031 0.0012 0.0004 0.0029 0.005
Nucleolus 0.0128 0.0114 0.0148 0.0091 0.103 0.0447 0.0245 0.0139 0.0227 0.0147 0.0439 0.0357 0.0086 0.0119 0.0162 0.0615 0.0429 0.0196
Nucleus 0.7316 0.972 0.9438 0.6367 0.8927 0.9245 0.791 0.9743 0.8827 0.7978 0.7158 0.9406 0.7714 0.9719 0.8947 0.9375 0.9388 0.9135
Peroxisomes 0.027 0 0.0012 0.0495 0 0.0016 0.016 0.0001 0.0163 0.0112 0.0333 0.0005 0.0041 0 0.0242 0 0 0.0008
Punctate Nuclear 0.0153 0.0137 0.0013 0.0384 0.0012 0.0112 0.0115 0.002 0.0044 0.003 0.0144 0.0014 0.0081 0.009 0.0058 0.0002 0.0142 0.0009
Vacuole 0.0036 0 0.0003 0.0017 0.0002 0.0002 0.002 0.0001 0.0011 0.0063 0.0046 0.0002 0.0033 0.0001 0.0001 0 0 0.0006
Vacuole Periphery 0.002 0 0.0001 0.0016 0 0.0001 0.0007 0 0.0005 0.003 0.0035 0.0002 0.0017 0 0.0001 0 0 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.034 15.175 17.2661 15.8356 8.0701 10.1796 13.1698 16.9145 17.6584 13.0862
Translational Efficiency 1.1242 1.5344 1.0677 1.2635 1.5333 1.102 1.4739 1.0846 1.0109 1.0258

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
154 1069 114 1081 1911 1252 229 119 2065 2321 343 1200

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.02 829.81 1107.42 948.87 782.52 802.96 966.70 985.89 773.68 815.33 1013.47 952.54
Standard Deviation 109.76 126.40 152.21 137.89 98.10 113.29 128.12 134.60 103.80 120.26 151.83 138.01
Intensity Change Log 2 — 0.321554 0.737904 0.514984 — 0.037200 0.304941 0.333299 — 0.174715 0.519893 0.419548

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000240 0.000693 0.001399 0.001299 0.000277 0.000585 0.001019 0.004531 0.000275 0.000635 0.001145 0.001620
Bud Neck 0.002649 0.014784 0.010055 0.027578 0.004976 0.010019 0.005495 0.011530 0.004802 0.012214 0.007011 0.025987
Bud Site 0.000695 0.001875 0.001380 0.012048 0.000415 0.002106 0.001093 0.009246 0.000436 0.001999 0.001189 0.011770
Cell Periphery 0.000103 0.000133 0.000046 0.000274 0.000061 0.000082 0.000082 0.000318 0.000065 0.000105 0.000070 0.000279
Cytoplasm 0.026239 0.008008 0.000380 0.018140 0.002628 0.004774 0.003538 0.018209 0.004389 0.006263 0.002489 0.018147
Cytoplasmic Foci 0.003728 0.000649 0.000025 0.003541 0.000481 0.001464 0.000171 0.007693 0.000723 0.001089 0.000122 0.003953
Eisosomes 0.000031 0.000024 0.000057 0.000034 0.000018 0.000027 0.000046 0.000055 0.000019 0.000025 0.000050 0.000036
Endoplasmic Reticulum 0.001563 0.005292 0.005335 0.005826 0.002168 0.004390 0.005174 0.006577 0.002123 0.004806 0.005227 0.005901
Endosome 0.000341 0.000558 0.000133 0.007778 0.000468 0.001596 0.000376 0.024685 0.000458 0.001118 0.000295 0.009454
Golgi 0.000252 0.000202 0.000080 0.003407 0.000196 0.000741 0.000097 0.002004 0.000200 0.000492 0.000091 0.003268
Lipid Particles 0.000689 0.000367 0.000104 0.000784 0.000318 0.000936 0.000292 0.002857 0.000346 0.000674 0.000230 0.000990
Mitochondria 0.000851 0.002269 0.001357 0.008611 0.000802 0.004221 0.000913 0.004376 0.000806 0.003322 0.001061 0.008191
Mitotic Spindle 0.004248 0.003039 0.000138 0.027042 0.001686 0.005282 0.001376 0.058136 0.001877 0.004249 0.000964 0.030126
None 0.026503 0.005442 0.000630 0.015062 0.003868 0.005087 0.011371 0.009961 0.005556 0.005250 0.007801 0.014556
Nuclear Periphery 0.000270 0.000948 0.000234 0.002334 0.000351 0.001014 0.000305 0.001081 0.000345 0.000984 0.000282 0.002209
Nuclear Periphery Foci 0.001036 0.000612 0.000031 0.002375 0.000317 0.001682 0.000190 0.001374 0.000370 0.001189 0.000137 0.002276
Nucleolus 0.068903 0.017547 0.009288 0.004451 0.035191 0.030698 0.011283 0.003588 0.037705 0.024641 0.010620 0.004365
Nucleus 0.860092* 0.934018* 0.966763* 0.845818* 0.944876* 0.920282* 0.954504* 0.799824* 0.938554* 0.926609* 0.958578* 0.841257*
Peroxisomes 0.000640 0.000679 0.000792 0.000832 0.000515 0.001046 0.000362 0.012115 0.000525 0.000877 0.000505 0.001951
Vacuole 0.000692 0.001803 0.001640 0.009063 0.000274 0.002377 0.002156 0.020407 0.000306 0.002113 0.001984 0.010188
Vacuole Periphery 0.000235 0.001061 0.000134 0.003704 0.000113 0.001591 0.000157 0.001433 0.000122 0.001347 0.000150 0.003479

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.89 -9.26 -13.59 -8.08 0.42 -4.01 -8.73 -4.21 -3.82 -2.89 -6.83 -11.91 -12.64 -9.04 -2.18
Bud Neck -9.51 -5.67 -15.38 -7.43 -7.43 -5.86 -3.13 -7.30 -3.46 -4.21 -10.16 -5.39 -15.36 -9.80 -10.78
Bud Site -3.16 -3.65 -6.17 -4.96 -5.18 -4.00 -5.15 -5.21 -3.91 -4.41 -5.62 -6.94 -7.20 -5.39 -5.92
Cell Periphery -0.76 2.26 -2.01 -1.31 -4.19 -1.75 -1.90 -3.69 -3.15 -2.83 -2.84 -1.13 -4.27 -2.68 -3.68
Cytoplasm 2.14 3.20 1.59 -2.02 -7.38 -2.50 -0.75 -2.44 -1.98 -2.25 -2.20 1.77 -4.93 -3.58 -5.99
Cytoplasmic Foci 1.68 2.27 1.65 -0.09 -2.49 -2.52 1.90 -1.00 -0.01 -1.27 -1.69 3.18 -0.84 0.47 -2.51
Eisosomes 2.09 -3.84 -2.14 -8.99 2.97 -5.24 -7.91 -7.62 -6.50 -3.29 -4.89 -10.33 -15.02 -10.93 2.02
Endoplasmic Reticulum -11.42 -7.66 -15.40 -4.62 -3.67 -6.78 -8.21 -7.33 -5.71 -4.49 -11.69 -10.31 -17.08 -8.11 -4.69
Endosome -1.79 2.60 -3.11 -2.84 -3.39 -2.35 -0.05 -1.31 -1.03 -1.31 -2.06 0.35 -3.11 -2.31 -3.32
Golgi 0.80 2.04 -1.99 -2.22 -2.49 -1.93 0.80 -1.49 0.11 -1.70 -1.77 1.21 -2.31 -1.51 -2.55
Lipid Particles 1.57 2.88 1.21 -0.39 -2.62 -1.63 0.02 -2.36 -1.13 -2.37 -1.53 1.11 -1.46 0.60 -2.41
Mitochondria -3.48 -3.50 -4.27 -2.70 -3.56 -3.76 -1.87 -3.92 0.93 -3.45 -4.78 -3.37 -4.66 -1.49 -4.14
Mitotic Spindle 0.30 1.02 -3.21 -5.69 -7.10 -2.36 -0.56 -2.94 -2.66 -2.80 -2.15 -0.08 -7.00 -6.14 -6.41
None 3.68 4.45 3.10 -2.31 -6.25 -1.33 -1.20 -2.09 -1.32 0.32 0.62 -0.17 -2.11 -2.61 -0.76
Nuclear Periphery -5.86 0.15 -7.16 -4.88 -7.22 -4.11 -0.99 -6.52 -3.87 -6.30 -6.14 -0.41 -7.59 -5.20 -7.55
Nuclear Periphery Foci 0.75 1.80 0.08 -1.10 -3.20 -1.66 0.20 -1.51 -0.63 -1.53 -1.74 1.34 -2.34 -0.02 -2.84
Nucleolus 5.65 6.48 7.07 8.24 2.82 1.54 8.17 10.89 8.47 2.11 6.00 11.33 18.36 14.12 4.18
Nucleus -4.42 -6.25 -1.02 7.84 12.61 5.02 -0.42 5.32 4.11 5.26 3.40 -2.71 10.47 8.15 10.98
Peroxisomes -0.46 -0.49 1.16 1.77 2.19 -1.98 0.50 -1.30 -0.99 -1.35 -1.81 -0.00 -0.37 1.20 -0.44
Vacuole -2.83 -3.42 -8.55 -7.44 -7.68 -3.80 -3.29 -3.96 -3.71 -3.72 -5.29 -4.25 -9.26 -7.71 -7.56
Vacuole Periphery -1.65 1.21 -2.36 -0.48 -2.58 -2.50 -1.17 -1.86 1.62 -1.55 -3.14 -1.24 -2.75 0.13 -2.63
Standard name
Human Ortholog
Description Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tfc1

Tfc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available