Standard name
Human Ortholog
Description Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.99 0.96 0.96 0.83 0.73 0.77 0.71 0.67 0.69 0.62 1.0 0.99 1.0 0.97 0.97 0.95 0.92 0.94 0.91 0.93 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0.11 0.36 0.44 0.39 0.41 0.53 0.51 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0 0.06 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0
Bud 0 0 1 1 1 8 18 3 17 25 24 28 0 0 0 0 1 0 0 0 2 1 2 1
Bud Neck 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 1 6 5 8 5 8 5 3 5 6 0 3 3 4 7 0 0 1 0 0 0
Cytoplasm 175 307 446 305 422 412 486 482 404 457 333 413 413 263 346 222 315 276 167 296 405 215 256 218
Endoplasmic Reticulum 0 4 1 2 0 2 3 0 3 4 1 3 0 0 0 5 9 2 0 0 3 1 0 0
Endosome 0 0 1 1 2 1 1 2 3 2 1 0 0 0 0 0 0 1 5 4 2 1 0 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 0 0 0
Mitochondria 7 12 3 43 48 178 291 243 236 360 245 366 0 0 0 1 4 5 0 3 7 2 3 0
Nucleus 1 0 2 1 0 0 6 4 4 6 3 1 1 0 1 3 3 2 0 0 2 1 0 0
Nuclear Periphery 0 0 1 1 0 1 6 3 1 1 2 3 0 0 0 1 0 0 0 1 1 1 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 1 1 4 1 0 0
Vac/Vac Membrane 0 1 2 0 4 3 26 24 27 17 30 60 0 2 0 5 6 14 1 1 1 1 3 2
Unique Cell Count 181 316 451 319 440 495 664 626 569 679 484 670 413 266 347 229 324 292 182 315 448 231 270 229
Labelled Cell Count 183 326 462 356 483 611 845 766 704 882 644 884 420 266 351 241 342 308 182 315 448 231 270 229


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.0 4.7 4.4 4.6 3.9 3.8 3.8 3.9 3.9 3.5 3.7 6.4 6.4 6.1 7.8 7.5 7.8 6.3 6.6 7.2
Std Deviation (1e-4) 0.7 0.7 1.2 1.3 1.3 0.8 1.0 0.8 1.0 0.9 0.9 0.7 0.8 0.9 0.9 1.4 1.3 1.4 1.1 1.2 1.3
Intensity Change (Log2) – – – -0.1 -0.02 -0.27 -0.29 -0.29 -0.25 -0.26 -0.4 -0.33 0.46 0.47 0.38 0.74 0.68 0.75 0.44 0.51 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.9 -2.8 -8.3 -11.4 -10.3 -11.9 -13.0 -12.3 -14.5 2.1 0 1.3 -1.8 -1.7 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.6 3.6 4.1 2.6 4.8 6.1 0 0 0 0 0 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2359 3.086 2.6572 2.9007 2.9371 2.873 2.211 2.41 2.6473 2.3726 3.0471 2.3755 0.7048 1.4185 0.7515 1.6202 0.5765 0.93
Actin 0.0222 0.0014 0.0061 0.0012 0.0014 0.0007 0.0451 0.0056 0.0153 0.0022 0.021 0.0077 0.029 0.0117 0.005 0.0376 0.004 0.0134
Bud 0.0016 0.0004 0.0055 0.0002 0.0005 0.0071 0.0026 0.0021 0.0025 0.0016 0.0014 0.0018 0.001 0.0037 0.0028 0.0022 0.0006 0.0016
Bud Neck 0.001 0.0005 0.0016 0.0006 0.0005 0.0106 0.0035 0.0004 0.0087 0.001 0.0068 0.0014 0.0018 0.0006 0.0017 0.0025 0.0008 0.0061
Bud Periphery 0.0018 0.0003 0.002 0.0001 0.0005 0.0021 0.0023 0.0019 0.0087 0.0034 0.0023 0.003 0.0012 0.0026 0.0038 0.0033 0.0018 0.0022
Bud Site 0.0022 0.0037 0.0103 0.0003 0.0007 0.0005 0.0049 0.014 0.0111 0.0011 0.0035 0.0004 0.0029 0.0146 0.0135 0.0083 0.0007 0.0012
Cell Periphery 0.0002 0.0001 0.0004 0 0.0001 0.0002 0.0003 0.0003 0.0004 0.0001 0.0002 0.0002 0.0002 0.0004 0.0007 0.0003 0.0001 0.0001
Cytoplasm 0.5237 0.6807 0.5542 0.5435 0.6447 0.6754 0.5031 0.6851 0.6458 0.6157 0.5822 0.7647 0.5686 0.667 0.666 0.5597 0.6617 0.7554
Cytoplasmic Foci 0.016 0.0056 0.0125 0.0169 0.0071 0.0097 0.0192 0.0106 0.0107 0.023 0.0247 0.0174 0.0218 0.0116 0.0096 0.0275 0.02 0.016
Eisosomes 0.0007 0 0.0001 0 0 0 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0049 0.0014 0.0031 0.0011 0.0023 0.0018 0.0063 0.0023 0.0077 0.0038 0.011 0.0036 0.0054 0.0017 0.003 0.0088 0.0026 0.0028
Endosome 0.0091 0.0015 0.0055 0.0036 0.0062 0.0026 0.0345 0.0035 0.0184 0.0196 0.0435 0.0069 0.024 0.0047 0.0037 0.0632 0.0185 0.0059
Golgi 0.0026 0.0003 0.0008 0.0009 0.0005 0.0003 0.0069 0.0009 0.0022 0.0009 0.0066 0.0006 0.0078 0.0016 0.0008 0.0236 0.0041 0.002
Lipid Particles 0.0049 0.0001 0.0017 0.0034 0.0002 0.001 0.0055 0.0008 0.0021 0.0008 0.0022 0.0003 0.0147 0.0006 0.0011 0.0089 0.0099 0.0023
Mitochondria 0.0009 0.0002 0.0009 0.0004 0.0011 0.0011 0.0057 0.0019 0.0022 0.001 0.0105 0.0012 0.0068 0.0013 0.0008 0.0137 0.0227 0.0008
None 0.3895 0.2972 0.3665 0.3867 0.3186 0.2768 0.3103 0.259 0.2384 0.2846 0.1973 0.1781 0.257 0.2645 0.2727 0.1651 0.223 0.1763
Nuclear Periphery 0.0038 0.0007 0.0024 0.0022 0.0024 0.0007 0.0136 0.0008 0.0071 0.0067 0.0396 0.0021 0.0158 0.0008 0.0015 0.0184 0.0056 0.0032
Nucleolus 0.0004 0.0001 0.0005 0.0005 0.0005 0.0004 0.0006 0.0003 0.0011 0.0015 0.0009 0.0001 0.0008 0.0002 0.0003 0.0036 0.0019 0.0001
Nucleus 0.0036 0.0035 0.0117 0.0019 0.008 0.003 0.0161 0.0035 0.008 0.0162 0.0146 0.0039 0.0108 0.0046 0.0044 0.0207 0.0037 0.0041
Peroxisomes 0.0034 0.0001 0.0047 0.002 0.0002 0.0018 0.0085 0.0016 0.001 0.0027 0.0053 0.0017 0.0212 0.0042 0.0038 0.016 0.006 0.0029
Punctate Nuclear 0.0059 0.0014 0.0077 0.0333 0.0027 0.0034 0.0048 0.0034 0.0045 0.0099 0.0059 0.003 0.0035 0.0012 0.0027 0.0091 0.0086 0.0024
Vacuole 0.001 0.0008 0.0013 0.001 0.0014 0.0008 0.0041 0.0019 0.0032 0.0034 0.0115 0.0016 0.0039 0.002 0.0016 0.0051 0.0025 0.0009
Vacuole Periphery 0.0003 0.0001 0.0002 0.0002 0.0004 0.0001 0.0017 0.0002 0.0008 0.0007 0.009 0.0004 0.0015 0.0002 0.0002 0.0025 0.0012 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.0704 19.1934 20.4347 19.6669 13.3136 10.2629 17.719 19.0776 23.9298 19.3556
Translational Efficiency 0.6608 0.674 0.6505 0.7522 0.8424 1.0421 0.7141 0.635 0.6438 0.6914

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1747 204 1194 1267 1931 843 234 1590 3678 1047 1428 2857

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 739.60 860.60 866.82 865.69 709.78 792.08 988.77 935.61 723.94 805.43 886.80 904.60
Standard Deviation 78.05 92.38 105.09 118.39 82.12 90.61 102.84 124.93 81.58 94.92 114.04 126.92
Intensity Change Log 2 — 0.218598 0.228987 0.227105 — 0.158274 0.478263 0.398535 — 0.189372 0.356442 0.313603

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000191 0.000294 0.000149 0.001378 0.000082 0.000535 0.000286 0.001263 0.000134 0.000488 0.000172 0.001314
Bud Neck 0.010619 0.019684 0.004531 0.003631 0.006647 0.014341 0.001282 0.006822 0.008534 0.015382 0.003999 0.005407
Bud Site 0.006169 0.016881 0.008476 0.033332 0.003113 0.014522 0.002850 0.038559 0.004565 0.014981 0.007554 0.036241
Cell Periphery 0.000109 0.000167 0.000153 0.000200 0.000109 0.000091 0.000172 0.000135 0.000109 0.000106 0.000156 0.000164
Cytoplasm 0.529029* 0.410982* 0.607089* 0.584360* 0.636634* 0.537058* 0.454195* 0.537806* 0.585523* 0.512493* 0.582035* 0.558451*
Cytoplasmic Foci 0.240475* 0.262250* 0.022274 0.032860 0.154064 0.231400* 0.022416 0.026289 0.195108* 0.237411* 0.022298 0.029203
Eisosomes 0.000130 0.000113 0.000035 0.000053 0.000074 0.000110 0.000072 0.000037 0.000101 0.000111 0.000041 0.000044
Endoplasmic Reticulum 0.001201 0.001417 0.001321 0.002880 0.001197 0.000460 0.003936 0.001506 0.001199 0.000647 0.001749 0.002116
Endosome 0.013678 0.036801 0.003488 0.004401 0.006650 0.019594 0.004130 0.012788 0.009988 0.022947 0.003593 0.009069
Golgi 0.003298 0.007379 0.000167 0.005801 0.001251 0.006491 0.000082 0.009373 0.002223 0.006664 0.000153 0.007789
Lipid Particles 0.006687 0.006906 0.001059 0.001746 0.003931 0.002986 0.002083 0.002494 0.005240 0.003750 0.001226 0.002162
Mitochondria 0.003768 0.004070 0.000416 0.003337 0.002254 0.004018 0.000138 0.008149 0.002973 0.004028 0.000371 0.006015
Mitotic Spindle 0.002764 0.005181 0.003396 0.017381 0.001326 0.007121 0.000484 0.059481 0.002009 0.006743 0.002919 0.040811
None 0.001743 0.000991 0.006927 0.003999 0.003237 0.002145 0.003540 0.002666 0.002527 0.001920 0.006372 0.003257
Nuclear Periphery 0.000319 0.000429 0.000851 0.000629 0.000443 0.000172 0.001284 0.001737 0.000384 0.000222 0.000922 0.001246
Nuclear Periphery Foci 0.000463 0.001094 0.001318 0.004300 0.000831 0.000453 0.004978 0.002916 0.000656 0.000578 0.001918 0.003530
Nucleolus 0.000656 0.001083 0.000697 0.000221 0.000514 0.000589 0.000196 0.000641 0.000581 0.000685 0.000615 0.000455
Nucleus 0.098610 0.067791 0.270254* 0.118076 0.131990* 0.062934 0.291671* 0.104192 0.116135 0.063881 0.273764* 0.110349
Peroxisomes 0.004333 0.010009 0.000276 0.001950 0.001345 0.007438 0.000182 0.002056 0.002765 0.007939 0.000260 0.002009
Vacuole 0.074569 0.144052 0.066834 0.178767 0.043354 0.086101 0.205896* 0.173771 0.058181 0.097392 0.089622 0.175987
Vacuole Periphery 0.001187 0.002428 0.000289 0.000699 0.000955 0.001440 0.000129 0.007318 0.001065 0.001633 0.000263 0.004382

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.57 0.21 -4.48 -3.79 -4.68 -1.83 -6.15 -2.52 -0.08 -0.66 -1.75 -3.51 -4.49 -0.91 -3.78
Bud Neck -2.06 5.02 8.10 4.01 1.49 -4.98 6.08 2.98 6.95 -3.63 -4.54 4.96 7.77 8.35 0.44
Bud Site -3.49 -1.02 -5.49 -0.68 -4.47 -5.32 -1.96 -6.80 -0.81 -6.30 -5.60 -2.22 -8.63 -1.27 -5.87
Cell Periphery -2.42 -3.36 -3.76 0.52 -0.17 2.10 -4.88 3.41 0.17 5.95 0.34 -4.94 -1.49 -1.35 3.81
Cytoplasm 5.84 -2.26 5.26 -3.15 6.78 9.08 15.79 20.23 6.62 -4.98 7.53 7.42 17.80 4.56 6.19
Cytoplasmic Foci -1.31 42.67 41.37 17.08 -3.67 -9.44 34.00 36.47 29.53 1.00 -5.75 52.94 53.88 33.54 -0.89
Eisosomes 1.66 13.42 11.08 5.83 -5.25 -2.04 -2.95 11.34 3.93 8.08 -0.63 12.90 14.88 4.41 0.39
Endoplasmic Reticulum -0.58 -4.03 -6.58 -3.36 -3.58 4.50 -7.85 -1.59 -11.83 7.60 4.10 -7.82 -5.89 -11.77 3.31
Endosome -5.46 11.51 13.34 8.28 1.29 -7.50 4.10 2.74 8.38 -0.21 -7.86 11.21 10.91 11.82 -0.37
Golgi -1.16 8.12 1.13 1.47 -2.02 -3.38 6.73 -1.92 2.21 -3.59 -3.09 10.03 -0.31 2.74 -3.88
Lipid Particles -0.00 11.27 10.02 3.02 -3.71 2.16 3.45 5.06 3.19 2.49 3.13 12.73 10.39 4.63 -0.99
Mitochondria -0.07 3.92 2.33 1.30 -1.69 -1.22 3.97 -0.38 1.01 -4.49 -0.80 5.26 1.51 1.66 -4.22
Mitotic Spindle -0.59 -0.62 -3.56 -1.11 -3.24 -2.31 1.36 -7.68 -4.69 -8.08 -2.17 -1.23 -8.41 -4.20 -7.72
None 1.50 -3.73 -1.46 -3.33 2.87 1.49 0.86 0.41 -1.31 -0.61 1.02 -3.23 -1.16 -1.65 2.79
Nuclear Periphery -1.47 -14.58 -10.84 -5.37 3.59 1.41 -5.85 -4.28 -7.41 0.29 1.56 -7.79 -6.13 -9.67 -0.42
Nuclear Periphery Foci -4.02 -8.28 -5.93 -4.40 -3.36 1.87 -8.53 -3.40 -5.37 6.02 0.51 -9.10 -6.83 -7.34 -1.05
Nucleolus -1.83 0.13 5.71 4.70 2.50 -0.83 6.41 2.07 2.06 -1.46 -0.99 0.07 5.17 4.02 1.94
Nucleus 3.58 -25.42 -7.66 -7.72 19.29 16.36 -9.79 -1.94 -16.33 9.03 14.91 -25.87 -6.59 -19.14 21.74
Peroxisomes -1.89 8.05 5.91 3.30 -1.83 -4.35 7.89 3.45 4.85 -3.76 -3.95 9.87 6.79 5.75 -3.16
Vacuole -6.04 -4.48 -28.62 -10.89 -23.32 -10.73 -15.11 -36.92 -27.85 -0.52 -9.86 -13.75 -46.31 -32.06 -24.33
Vacuole Periphery -2.21 5.67 4.23 3.87 -0.44 -1.56 4.06 -2.25 -1.34 -4.17 -2.25 6.01 -0.86 0.82 -3.92
Standard name
Human Ortholog
Description Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ptc4

Ptc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available