Standard name | |
---|---|
Human Ortholog | |
Description | Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress; human homolog ATP6V1B1, implicated in autosomal-recessive distal renal tubular acidosis (RTA) with sensorineural deafness, complements yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 3 | 6 | 5 | 22 | 31 | 19 | 33 | 26 | 47 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 1 | 2 | 0 | 0 | 3 | 6 | 2 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 5 | 2 | 1 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 3 | 12 | 27 | 22 | 13 | 37 | 29 | 45 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 135 | 154 | 102 | 130 | 145 | 149 | 195 | 161 | 176 | 194 | 192 | 160 | 37 | 20 | 33 | 88 | 92 | 56 | 42 | 53 | 8 | 4 | 3 | 0 |
Endoplasmic Reticulum | 16 | 16 | 24 | 19 | 24 | 47 | 137 | 196 | 80 | 117 | 51 | 70 | 2 | 4 | 14 | 9 | 8 | 5 | 7 | 3 | 10 | 0 | 2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 9 | 15 | 39 | 9 | 12 | 16 |
Golgi | 18 | 3 | 179 | 108 | 143 | 77 | 99 | 105 | 50 | 91 | 58 | 103 | 42 | 34 | 74 | 39 | 81 | 53 | 1 | 1 | 3 | 2 | 0 | 6 |
Mitochondria | 0 | 0 | 0 | 2 | 1 | 12 | 22 | 14 | 13 | 37 | 70 | 59 | 0 | 0 | 0 | 5 | 5 | 2 | 0 | 1 | 2 | 0 | 0 | 1 |
Nucleus | 0 | 1 | 0 | 0 | 4 | 1 | 1 | 0 | 3 | 0 | 0 | 4 | 0 | 0 | 5 | 4 | 0 | 1 | 43 | 42 | 8 | 18 | 24 | 21 |
Nuclear Periphery | 1 | 0 | 2 | 0 | 1 | 7 | 11 | 14 | 7 | 7 | 3 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 12 | 62 | 8 | 11 | 10 |
Nucleolus | 4 | 5 | 14 | 8 | 10 | 9 | 16 | 14 | 0 | 10 | 2 | 2 | 4 | 3 | 2 | 6 | 9 | 3 | 9 | 6 | 7 | 9 | 10 | 11 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 1 |
Vac/Vac Membrane | 392 | 294 | 487 | 190 | 278 | 242 | 248 | 197 | 166 | 198 | 154 | 187 | 275 | 299 | 381 | 207 | 214 | 167 | 479 | 455 | 553 | 188 | 320 | 373 |
Unique Cell Count | 530 | 458 | 635 | 384 | 509 | 513 | 684 | 662 | 473 | 618 | 515 | 559 | 305 | 324 | 428 | 306 | 319 | 230 | 626 | 606 | 709 | 246 | 390 | 452 |
Labelled Cell Count | 567 | 473 | 812 | 463 | 616 | 561 | 781 | 760 | 529 | 727 | 588 | 688 | 360 | 361 | 510 | 360 | 411 | 288 | 626 | 606 | 709 | 246 | 390 | 452 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 66.7 | 69.1 | 50.6 | 49.1 | 45.6 | 38.1 | 36.2 | 33.9 | 34.6 | 32.0 | 32.0 | 32.0 | 65.5 | 63.5 | 64.9 | 39.9 | 49.8 | 58.3 | 59.9 | 65.7 | 69.2 |
Std Deviation (1e-4) | 14.9 | 17.1 | 13.4 | 11.0 | 12.1 | 10.1 | 10.0 | 8.3 | 8.3 | 7.7 | 8.5 | 7.8 | 15.3 | 14.8 | 15.8 | 15.7 | 19.5 | 20.2 | 16.6 | 18.0 | 20.5 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.15 | -0.41 | -0.48 | -0.58 | -0.55 | -0.66 | -0.66 | -0.66 | 0.37 | 0.33 | 0.36 | -0.34 | -0.02 | 0.2 | 0.24 | 0.38 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.9 | 5.0 | 4.5 | 5.4 | 5.2 | 7.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 3.5 | 4.8 | 4.3 | 3.8 | 6.0 | 5.8 | 7.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.6 | 5.1 | 5.3 | 5.4 | 3.7 | 8.0 | 6.4 | 8.2 | 5.2 | -1.6 | -4.3 | -4.0 | 4.5 | 4.6 | 2.8 |
Endoplasmic Reticulum | 0.9 | 0.8 | 3.8 | 9.0 | 12.4 | 7.4 | 8.5 | 4.2 | 5.6 | -2.7 | -2.2 | -0.4 | -0.7 | -1.0 | -1.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | -5.3 | -6.1 | -5.4 | -7.2 | -5.8 | -7.1 | -4.0 | -4.9 | -6.2 | -4.1 | -5.3 | -0.9 | -1.5 |
Mitochondria | 0 | 0 | 3.9 | 4.6 | 3.7 | 4.2 | 6.3 | 9.6 | 8.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.4 | 2.9 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.1 | -0.3 | -0.5 | 0.2 | -0.1 | -3.2 | -0.8 | -2.6 | -2.8 | -0.9 | -1.4 | -2.3 | -0.2 | 0.6 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -8.9 | -7.9 | -10.3 | -14.8 | -16.9 | -13.9 | -15.9 | -15.9 | -15.0 | 4.9 | 5.9 | 5.1 | -3.0 | -3.2 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 35.4547 | 50.3972 | 48.2843 | 43.0025 | 48.1709 | 44.1119 | 50.9707 | 62.3837 | 56.0425 | 50.6955 | 45.2594 | 54.9061 | 59.7897 | 67.8797 | 63.002 | 61.8325 | 64.1985 | 63.1456 |
Actin | 0.026 | 0.0001 | 0.0108 | 0.0001 | 0 | 0 | 0.0027 | 0 | 0.0019 | 0 | 0.0005 | 0.001 | 0 | 0 | 0.0011 | 0 | 0 | 0 |
Bud | 0.0012 | 0.0014 | 0.0013 | 0.0014 | 0.0021 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0004 | 0.0001 |
Bud Neck | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Bud Periphery | 0.001 | 0.0005 | 0.0013 | 0.0004 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0009 | 0.0001 |
Bud Site | 0.0014 | 0.0014 | 0.001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0.0022 | 0.0016 | 0.0016 | 0.0008 | 0.0015 | 0.0013 | 0.0012 | 0.0005 | 0.0009 | 0.0004 | 0.0006 | 0.0005 | 0.0011 | 0.0013 | 0.001 | 0.0009 | 0.0007 | 0.0004 |
Cytoplasm | 0.0242 | 0.0782 | 0.0753 | 0.0855 | 0.0612 | 0.0136 | 0.0108 | 0.0388 | 0.0243 | 0.0017 | 0.0042 | 0.0044 | 0.0061 | 0.0285 | 0.0342 | 0.0007 | 0.0006 | 0.0095 |
Cytoplasmic Foci | 0.0173 | 0.002 | 0.0075 | 0.0161 | 0.0001 | 0.0004 | 0.0035 | 0.0001 | 0.0087 | 0.0001 | 0.0282 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0029 | 0.0004 | 0.0024 | 0.0031 | 0.0001 | 0.0003 | 0.0008 | 0.0004 | 0.0017 | 0.0001 | 0.0011 | 0.0006 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.0148 | 0.0023 | 0.0098 | 0.0148 | 0.0017 | 0.0165 | 0.0056 | 0.0025 | 0.009 | 0.0035 | 0.0053 | 0.0082 | 0.0026 | 0.0026 | 0.0036 | 0.0012 | 0.001 | 0.0023 |
Golgi | 0.0036 | 0.0001 | 0.0008 | 0.0004 | 0 | 0.0007 | 0.0006 | 0 | 0.0008 | 0 | 0.001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Lipid Particles | 0.0041 | 0.0002 | 0.0019 | 0.0041 | 0 | 0.0002 | 0.0032 | 0 | 0.003 | 0 | 0.012 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 |
Mitochondria | 0.0035 | 0.0006 | 0.0018 | 0.0004 | 0.0002 | 0.0013 | 0.0013 | 0.0001 | 0.0016 | 0 | 0.0091 | 0.0004 | 0.0006 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 |
None | 0.0157 | 0.0029 | 0.0094 | 0.0035 | 0 | 0.0002 | 0.0013 | 0 | 0.002 | 0 | 0.005 | 0.0001 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0177 | 0.0347 | 0.0076 | 0.0062 | 0.0038 | 0.0289 | 0.0297 | 0.0342 | 0.0311 | 0.0044 | 0.0103 | 0.0404 | 0.0175 | 0.0385 | 0.0216 | 0.0067 | 0.0107 | 0.0249 |
Nucleolus | 0.0045 | 0.0022 | 0.0019 | 0.0006 | 0.0041 | 0.0031 | 0.0018 | 0.0002 | 0.0051 | 0.0001 | 0.0196 | 0.0005 | 0.0029 | 0.0006 | 0.0008 | 0.0004 | 0.0002 | 0.0026 |
Nucleus | 0.02 | 0.0183 | 0.009 | 0.0052 | 0.0168 | 0.0195 | 0.0125 | 0.004 | 0.0055 | 0.0007 | 0.0317 | 0.0068 | 0.0119 | 0.0039 | 0.0098 | 0.0009 | 0.0014 | 0.0035 |
Peroxisomes | 0.0009 | 0.0001 | 0.0006 | 0.0002 | 0 | 0 | 0.0019 | 0 | 0.0009 | 0 | 0.0014 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0059 | 0.0015 | 0.0004 | 0.0006 | 0 | 0 | 0.0013 | 0 | 0.0025 | 0 | 0.0039 | 0.0014 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Vacuole | 0.4052 | 0.42 | 0.453 | 0.3827 | 0.4643 | 0.3323 | 0.3251 | 0.362 | 0.3389 | 0.3004 | 0.1677 | 0.2397 | 0.3479 | 0.3677 | 0.3732 | 0.3114 | 0.2708 | 0.24 |
Vacuole Periphery | 0.4274 | 0.4314 | 0.4021 | 0.4736 | 0.4429 | 0.5809 | 0.5956 | 0.5569 | 0.5596 | 0.6885 | 0.6964 | 0.6946 | 0.6071 | 0.5555 | 0.5504 | 0.6776 | 0.7128 | 0.7161 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 627.6767 | 573.1645 | 682.0077 | 576.3194 | 590.1045 | 618.1037 | 558.9892 | 547.944 | 603.247 | 525.6974 |
Translational Efficiency | 1.3371 | 1.5121 | 1.2127 | 1.3924 | 1.3507 | 1.2921 | 1.3352 | 1.1476 | 1.1652 | 1.3507 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
104 | 1040 | 782 | 1293 | 1783 | 1464 | 881 | 154 | 1887 | 2504 | 1663 | 1447 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 2578.76 | 4230.42 | 5874.14 | 5100.10 | 3306.22 | 3701.74 | 5333.02 | 4405.35 | 3266.13 | 3921.32 | 5587.47 | 5026.16 |
Standard Deviation | 618.51 | 1106.07 | 1191.55 | 1507.88 | 815.60 | 882.30 | 1156.99 | 1076.76 | 822.91 | 1015.44 | 1204.05 | 1483.59 |
Intensity Change Log 2 | — | 0.714123 | 1.187700 | 0.983848 | — | 0.163021 | 0.689770 | 0.414074 | — | 0.430676 | 0.929311 | 0.691717 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000538 | 0.000032 | 0.000031 | 0.000325 | 0.000007 | 0.000019 | 0.000054 | 0.000070 | 0.000037 | 0.000024 | 0.000043 | 0.000298 |
Bud Neck | 0.002137 | 0.000428 | 0.000828 | 0.001861 | 0.000090 | 0.000126 | 0.001386 | 0.003286 | 0.000203 | 0.000252 | 0.001124 | 0.002012 |
Bud Site | 0.005036 | 0.001871 | 0.000225 | 0.001791 | 0.000557 | 0.000613 | 0.000680 | 0.002203 | 0.000804 | 0.001135 | 0.000466 | 0.001834 |
Cell Periphery | 0.002293 | 0.000516 | 0.000356 | 0.001489 | 0.000540 | 0.000389 | 0.000521 | 0.000429 | 0.000636 | 0.000441 | 0.000444 | 0.001376 |
Cytoplasm | 0.000998 | 0.012030 | 0.000229 | 0.001776 | 0.033004 | 0.004270 | 0.000152 | 0.001065 | 0.031240 | 0.007493 | 0.000188 | 0.001701 |
Cytoplasmic Foci | 0.012304 | 0.003959 | 0.002611 | 0.008824 | 0.000660 | 0.000645 | 0.001593 | 0.006874 | 0.001302 | 0.002021 | 0.002072 | 0.008616 |
Eisosomes | 0.000022 | 0.000002 | 0.000007 | 0.000019 | 0.000000 | 0.000001 | 0.000019 | 0.000011 | 0.000002 | 0.000001 | 0.000013 | 0.000018 |
Endoplasmic Reticulum | 0.005516 | 0.000080 | 0.000017 | 0.000255 | 0.000138 | 0.000103 | 0.000066 | 0.000072 | 0.000435 | 0.000094 | 0.000043 | 0.000235 |
Endosome | 0.010530 | 0.000789 | 0.000118 | 0.002265 | 0.000363 | 0.000321 | 0.000247 | 0.004428 | 0.000923 | 0.000515 | 0.000187 | 0.002495 |
Golgi | 0.020482 | 0.017888 | 0.007368 | 0.021239 | 0.012021 | 0.007749 | 0.003511 | 0.011704 | 0.012487 | 0.011960 | 0.005325 | 0.020224 |
Lipid Particles | 0.003176 | 0.000546 | 0.000315 | 0.003131 | 0.000209 | 0.000155 | 0.000428 | 0.002351 | 0.000373 | 0.000317 | 0.000375 | 0.003048 |
Mitochondria | 0.002206 | 0.000265 | 0.000336 | 0.004489 | 0.000139 | 0.000159 | 0.001119 | 0.000595 | 0.000253 | 0.000203 | 0.000751 | 0.004074 |
Mitotic Spindle | 0.006642 | 0.000719 | 0.000038 | 0.001161 | 0.000044 | 0.000039 | 0.000041 | 0.000298 | 0.000408 | 0.000321 | 0.000040 | 0.001069 |
None | 0.000151 | 0.000273 | 0.000100 | 0.000870 | 0.000298 | 0.000291 | 0.000170 | 0.000205 | 0.000290 | 0.000284 | 0.000137 | 0.000799 |
Nuclear Periphery | 0.001055 | 0.000260 | 0.001415 | 0.002501 | 0.000109 | 0.000261 | 0.001062 | 0.002516 | 0.000161 | 0.000261 | 0.001228 | 0.002503 |
Nuclear Periphery Foci | 0.004341 | 0.001326 | 0.000945 | 0.003687 | 0.000586 | 0.000446 | 0.000514 | 0.002828 | 0.000793 | 0.000811 | 0.000717 | 0.003595 |
Nucleolus | 0.002890 | 0.000398 | 0.003791 | 0.003893 | 0.000500 | 0.000378 | 0.006586 | 0.005224 | 0.000631 | 0.000387 | 0.005272 | 0.004035 |
Nucleus | 0.000739 | 0.000787 | 0.000293 | 0.001514 | 0.000981 | 0.000727 | 0.000873 | 0.001498 | 0.000967 | 0.000752 | 0.000601 | 0.001512 |
Peroxisomes | 0.001076 | 0.000015 | 0.000046 | 0.000224 | 0.000007 | 0.000006 | 0.000070 | 0.000155 | 0.000066 | 0.000010 | 0.000059 | 0.000216 |
Vacuole | 0.301462* | 0.191773 | 0.150640 | 0.252149* | 0.197461 | 0.150707 | 0.253058* | 0.346606* | 0.203193* | 0.167763 | 0.204898* | 0.262202* |
Vacuole Periphery | 0.616406* | 0.766043* | 0.830289* | 0.686540* | 0.752286* | 0.832596* | 0.727849* | 0.607583* | 0.744798* | 0.804955* | 0.776020* | 0.678137* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.84 | 1.86 | 0.67 | -3.38 | -3.43 | -1.13 | -5.94 | -3.04 | -2.46 | -1.13 | 0.63 | -0.53 | -3.39 | -3.57 | -3.34 |
Bud Neck | 2.15 | 1.55 | -0.10 | -5.15 | -3.33 | -1.06 | -4.47 | -3.26 | -3.24 | -2.15 | -0.88 | -4.92 | -6.78 | -6.64 | -3.34 |
Bud Site | 1.29 | 2.12 | 1.32 | 0.08 | -6.07 | -0.46 | -0.76 | -2.75 | -2.57 | -2.56 | -1.57 | 1.94 | -4.09 | -2.38 | -5.73 |
Cell Periphery | 0.94 | 1.04 | 0.41 | -4.19 | -5.12 | 1.68 | 0.18 | 1.12 | -0.54 | 0.99 | 1.45 | 1.53 | -3.32 | -4.63 | -4.71 |
Cytoplasm | -5.15 | 1.77 | -1.35 | 4.68 | -2.83 | 9.58 | 11.51 | 9.15 | 0.94 | -1.33 | 7.83 | 11.48 | 10.59 | 4.85 | -3.20 |
Cytoplasmic Foci | 1.84 | 2.19 | 0.40 | -3.32 | -4.37 | 0.20 | -2.93 | -2.48 | -2.49 | -2.23 | -1.65 | -1.86 | -6.18 | -5.46 | -5.47 |
Eisosomes | 2.64 | 1.93 | 0.38 | -7.94 | -4.67 | -3.70 | -9.56 | -4.86 | -4.63 | 2.49 | 0.67 | -8.90 | -8.38 | -8.74 | -2.25 |
Endoplasmic Reticulum | 1.06 | 1.08 | 1.03 | -4.77 | -7.02 | 1.27 | 4.90 | 2.74 | 0.84 | -0.68 | 1.20 | 1.39 | 0.70 | -4.18 | -6.34 |
Endosome | 2.60 | 2.80 | 2.19 | -2.36 | -3.66 | 0.45 | 1.34 | -1.62 | -1.64 | -1.67 | 1.65 | 3.32 | -2.54 | -3.31 | -3.92 |
Golgi | 0.50 | 2.86 | -0.15 | -1.33 | -6.85 | 3.87 | 8.99 | 0.04 | -2.06 | -4.32 | 0.36 | 7.51 | -4.17 | -4.52 | -8.66 |
Lipid Particles | 1.20 | 1.31 | 0.15 | -5.50 | -6.26 | 1.50 | -4.79 | -2.68 | -2.75 | -2.46 | 0.47 | 0.15 | -6.19 | -6.64 | -6.57 |
Mitochondria | 2.76 | 2.72 | -2.25 | -5.15 | -5.13 | -0.39 | -3.53 | -3.28 | -3.19 | 1.57 | 0.41 | -3.16 | -5.29 | -5.33 | -4.53 |
Mitotic Spindle | 1.45 | 1.68 | 1.38 | -0.57 | -3.42 | 0.30 | 0.14 | -1.49 | -1.52 | -1.50 | 0.05 | 1.68 | -1.91 | -2.03 | -3.52 |
None | -1.38 | 1.21 | -3.73 | -2.75 | -4.14 | 0.05 | 2.60 | 0.98 | 0.61 | -1.33 | -0.10 | 3.41 | -3.01 | -2.87 | -4.00 |
Nuclear Periphery | 3.00 | -1.00 | -3.71 | -7.63 | -2.95 | -2.22 | -3.33 | -2.84 | -2.67 | -1.68 | -2.11 | -5.67 | -8.53 | -8.09 | -3.89 |
Nuclear Periphery Foci | 1.11 | 1.25 | 0.32 | -3.76 | -5.07 | 1.35 | 0.73 | -2.32 | -2.45 | -2.39 | 0.00 | 0.51 | -5.53 | -5.60 | -6.00 |
Nucleolus | 1.88 | -0.49 | -0.83 | -6.70 | -0.31 | 0.47 | -5.39 | -3.05 | -3.14 | 0.08 | 0.92 | -5.76 | -6.32 | -7.44 | 0.98 |
Nucleus | -0.46 | 1.15 | -1.93 | -2.00 | -3.79 | 1.11 | 0.82 | -0.88 | -1.14 | -1.06 | 0.96 | 2.07 | -1.78 | -2.54 | -3.25 |
Peroxisomes | 1.53 | 1.49 | 1.20 | -2.57 | -2.17 | 0.47 | -3.23 | -2.36 | -2.37 | -1.56 | 1.45 | 0.18 | -1.94 | -2.88 | -2.23 |
Vacuole | 4.53 | 6.47 | 2.12 | -7.00 | -12.82 | 7.77 | -7.06 | -8.61 | -11.26 | -5.38 | 6.13 | -0.23 | -8.86 | -15.07 | -8.44 |
Vacuole Periphery | -5.04 | -7.64 | -2.40 | 7.35 | 15.59 | -11.12 | 2.43 | 7.53 | 11.58 | 6.39 | -8.39 | -4.46 | 8.20 | 16.72 | 12.73 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|