Standard name
Human Ortholog
Description Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.07 0.06 0.07 0.1 0.13 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.11 0.06 0.06 0.07 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.1 0.36 0.28 0.34 0.52 0.49 0.42 0.5 0.49 0.62 0.07 0.05 0.08 0.1 0.21 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.53 0.46 0.14 0.16 0.12 0.09 0.07 0.07 0.06 0 0 0.08 0.68 0.81 0.67 0.6 0.62 0.78 0.81 0.68 0.78 0.77 0.82 0.75
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.25 0 0 0 0.16 0.1 0.09 0.32 0.3 0.38 0.14 0.13 0.06 0.11 0.13 0 0.09 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.07 0 0 0 0 0 0 0 0 0 0 0.16 0.11 0.14 0.1 0.12 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0 0 0 0
SpindlePole 0.33 0.12 0.62 0.4 0.44 0.34 0.29 0.32 0.22 0.19 0.1 0.12 0.26 0.26 0.17 0.3 0.24 0.35 0.07 0.05 0.09 0.09 0.05 0
Vac/Vac Membrane 0.1 0.33 0.06 0 0.06 0 0 0 0 0 0.07 0.05 0.12 0.14 0.12 0.2 0.24 0.22 0 0.12 0 0.07 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 14 8 20 11 10 16 9 6 0 7 0 0 2 2 0 1 0 2 0 0 1 0
Bud 3 1 7 8 19 30 29 39 52 60 42 31 1 2 3 1 1 0 0 2 1 0 1 1
Bud Neck 0 0 28 19 25 27 27 33 25 16 7 21 1 0 0 0 1 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 1 2 2 2 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 0 1 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 21 27 116 111 139 253 267 209 229 221 311 17 12 20 6 7 0 2 5 3 0 1 4
Endoplasmic Reticulum 2 6 2 10 7 5 5 1 0 4 0 4 3 1 5 0 0 0 0 0 0 0 2 2
Endosome 128 95 37 51 49 36 34 38 28 16 17 38 170 206 175 36 21 18 217 159 332 87 148 173
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1 0 0 1
Mitochondria 59 52 5 12 16 65 48 47 160 137 173 72 32 15 29 8 1 2 7 11 17 1 2 7
Nucleus 2 3 1 0 1 4 0 4 3 3 1 2 0 1 3 0 0 0 1 0 0 0 0 0
Nuclear Periphery 3 2 1 1 0 0 0 0 0 0 0 1 3 2 7 2 0 1 3 2 0 0 0 1
Nucleolus 42 14 2 0 0 4 2 1 1 2 0 0 40 27 36 6 4 1 0 3 2 0 0 0
Peroxisomes 16 0 0 0 2 2 0 0 1 4 0 0 2 24 18 0 0 0 5 1 3 1 1 0
SpindlePole 79 25 163 129 175 140 141 177 111 86 47 63 64 67 45 18 8 8 18 12 40 9 8 10
Vac/Vac Membrane 25 67 17 10 22 13 9 24 15 11 32 26 29 36 30 12 8 5 4 27 17 8 10 24
Unique Cell Count 242 205 264 319 394 413 489 549 497 461 451 505 250 253 260 60 34 23 267 234 425 114 181 233
Labelled Cell Count 365 286 305 364 447 476 560 649 617 576 543 581 362 393 373 92 51 36 267 234 425 114 181 233


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.2 5.4 5.0 5.3 4.2 4.3 4.7 3.9 3.8 3.8 4.4 5.2 5.4 5.2 8.4 7.8 7.6 4.9 4.9 5.4
Std Deviation (1e-4) 0.5 0.9 0.5 0.8 0.8 0.7 0.7 0.9 0.8 0.6 0.9 1.0 1.1 1.2 1.0 1.6 1.1 0.7 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.12 -0.03 -0.37 -0.31 -0.2 -0.46 -0.52 -0.51 -0.28 -0.05 0.01 -0.04 0.64 0.54 0.49 -0.14 -0.14 0.01

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.8 -0.1 0 0 0 0 0 0 0 -3.7 -3.7 -3.0 0 0 0
Bud -0.1 1.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -2.1 -2.0 0 0 0 0 0 0 0 -5.0 -5.3 -5.4 -2.6 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.3 5.5 6.9 11.2 10.7 9.0 10.7 10.5 13.6 -1.4 -2.4 -1.0 -0.1 0 0
Endoplasmic Reticulum 2.0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.7 -0.6 0 0 0 0 0 0 0 12.5 15.4 12.4 7.7 6.6 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 1.6 0 0 0 0 0 0 0 4.8 2.4 4.3 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 6.3 4.9 5.8 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 5.1 4.4 0 0 0
SpindlePole -5.1 -4.4 0 0 0 0 0 0 0 -8.2 -8.1 -10.4 -4.5 -4.2 -2.5
Vacuole -1.9 -0.5 -2.0 -3.3 -1.3 -2.2 -2.7 0.3 -0.7 2.0 2.9 2.0 3.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9877 0.4696 0.5261 0.6293 0.2295 0.2886 0.1269 0.8028 0.6024 0.5232 0.5821 0.5734 0.4795 0.6706 0.355 0.556 0.5003 0.2606
Actin 0.0178 0.0001 0.0058 0.0409 0.0101 0.0001 0.0223 0 0.0004 0 0.0154 0.0001 0.0417 0.0002 0.0206 0.0002 0.0116 0.0002
Bud 0.0005 0.0001 0.0002 0.0003 0.0004 0 0.0003 0.0001 0.001 0 0.0002 0 0.0003 0.0001 0.0001 0.0013 0.0007 0
Bud Neck 0.0017 0.0004 0.0005 0.0004 0.0005 0.0005 0.0039 0.0004 0.0002 0.0002 0.0004 0.0003 0.0014 0.0003 0.0004 0.0006 0.0006 0.0002
Bud Periphery 0.0006 0.0001 0.0003 0.0004 0.0005 0.0001 0.0004 0.0001 0.0005 0 0.0004 0 0.0005 0.0002 0.0002 0.0025 0.0006 0
Bud Site 0.0021 0.001 0.001 0.0007 0.0024 0.0007 0.0034 0.0009 0.002 0.0001 0.0004 0.0001 0.002 0.0007 0.0005 0.0012 0.007 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0004 0.0002 0.0001 0.0001 0.0001 0
Cytoplasm 0.017 0.0252 0.0211 0.0022 0.0018 0.0048 0.0141 0.0139 0.0067 0.0014 0.0077 0.0042 0.0193 0.013 0.0064 0.013 0.0019 0.0031
Cytoplasmic Foci 0.2029 0.2353 0.2424 0.2261 0.279 0.2128 0.2635 0.2894 0.2857 0.3017 0.2894 0.3113 0.2691 0.2945 0.2739 0.2727 0.3899 0.259
Eisosomes 0.0001 0 0.0003 0.0001 0.0001 0 0.0002 0 0 0 0.0003 0 0.0004 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0113 0.0009 0.0019 0.0002 0.002 0.0006 0.003 0.0007 0.0011 0 0.0008 0.0003 0.0101 0.0011 0.0009 0.0005 0.0003 0.0004
Endosome 0.3846 0.4668 0.4233 0.5237 0.32 0.3819 0.3807 0.3535 0.3551 0.4209 0.388 0.3014 0.2888 0.3527 0.3404 0.4408 0.2271 0.2951
Golgi 0.0338 0.0241 0.0326 0.0286 0.0579 0.0295 0.0258 0.0279 0.0465 0.0347 0.036 0.0328 0.0253 0.0248 0.0309 0.0487 0.0394 0.0361
Lipid Particles 0.173 0.1497 0.15 0.1255 0.1905 0.2666 0.1711 0.1702 0.145 0.0403 0.1089 0.2112 0.1943 0.1851 0.1424 0.106 0.1096 0.2839
Mitochondria 0.0062 0.009 0.0077 0.0021 0.0098 0.001 0.0025 0.002 0.0044 0.0007 0.0538 0.0008 0.0046 0.0026 0.0079 0.0027 0.0384 0.0017
None 0.0104 0.008 0.027 0.0004 0.0014 0.017 0.0183 0.0014 0.0043 0.0001 0.0154 0.0015 0.044 0.036 0.0443 0.0142 0.0006 0.0119
Nuclear Periphery 0.0307 0.0009 0.0013 0.0003 0.0034 0.0006 0.0037 0.0003 0.0006 0.0001 0.0018 0.001 0.0039 0.0011 0.0006 0.0005 0.0003 0.0003
Nucleolus 0.0012 0.0009 0.0012 0.0003 0.0006 0.0016 0.0008 0.0006 0.0004 0.0002 0.0004 0.0003 0.0006 0.0008 0.0003 0.0005 0.0003 0.0003
Nucleus 0.0171 0.0008 0.0005 0.0001 0.0008 0.0018 0.0022 0.0004 0.0003 0.0001 0.0006 0.0011 0.0012 0.0006 0.0002 0.0007 0.0002 0.0001
Peroxisomes 0.0555 0.0335 0.06 0.0391 0.0961 0.0629 0.0682 0.1193 0.1338 0.159 0.0677 0.1164 0.0778 0.0507 0.1056 0.0792 0.1629 0.0951
Punctate Nuclear 0.0205 0.0095 0.0033 0.0012 0.0185 0.0033 0.0083 0.0044 0.0023 0.0371 0.0043 0.0123 0.0067 0.0128 0.013 0.0042 0.0062 0.0081
Vacuole 0.0093 0.0312 0.0168 0.0065 0.0029 0.0127 0.0057 0.0128 0.0068 0.0024 0.0055 0.0035 0.0064 0.0202 0.0095 0.0085 0.0014 0.0032
Vacuole Periphery 0.0035 0.0025 0.0027 0.0008 0.0013 0.0015 0.0014 0.0016 0.003 0.0007 0.0024 0.0014 0.0012 0.0022 0.0016 0.0019 0.001 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5213 6.9956 6.2292 6.9603 9.4698 5.7356 7.1911 7.7974 6.313 7.1837
Translational Efficiency 0.6858 0.6555 0.6451 0.5242 0.4404 0.7236 0.6415 0.4987 0.6091 0.676

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1524 455 1071 1135 2082 1136 841 2703 3606 1591 1912 3838

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.79 818.92 1024.07 931.62 779.96 757.09 972.00 933.83 743.96 774.77 1001.17 933.18
Standard Deviation 86.20 105.89 124.06 123.66 88.00 103.22 109.40 129.66 96.86 107.68 120.64 127.92
Intensity Change Log 2 — 0.237146 0.559665 0.423165 — -0.042935 0.317556 0.259760 — 0.095806 0.436692 0.339053

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000078 0.000181 0.000639 0.000938 0.000046 0.000246 0.000070 0.000283 0.000059 0.000227 0.000389 0.000477
Bud Neck 0.022685 0.008757 0.010387 0.015308 0.008838 0.009264 0.015973 0.062981 0.014690 0.009119 0.012844 0.048883
Bud Site 0.003767 0.008739 0.013909 0.038699 0.002556 0.007358 0.008697 0.022494 0.003068 0.007753 0.011617 0.027287
Cell Periphery 0.000093 0.000039 0.000050 0.000048 0.000035 0.000253 0.000028 0.000028 0.000059 0.000191 0.000040 0.000034
Cytoplasm 0.016343 0.015656 0.003203 0.035982 0.005374 0.013401 0.003542 0.081193 0.010010 0.014046 0.003352 0.067823
Cytoplasmic Foci 0.164500 0.116226 0.156856 0.168177 0.089227 0.149553 0.075022 0.113531 0.121039 0.140022 0.120861 0.129691
Eisosomes 0.000052 0.000025 0.000028 0.000019 0.000022 0.000046 0.000029 0.000016 0.000035 0.000040 0.000028 0.000017
Endoplasmic Reticulum 0.000198 0.000212 0.000905 0.000455 0.000299 0.000397 0.000773 0.000353 0.000256 0.000344 0.000847 0.000383
Endosome 0.630471 0.726274 0.429337 0.446806 0.790864 0.632752 0.650426 0.363702 0.723077 0.659498 0.526583 0.388278
Golgi 0.039408 0.023923 0.028688 0.017331 0.019489 0.054367 0.007418 0.011124 0.027907 0.045661 0.019332 0.012959
Lipid Particles 0.024361 0.011978 0.007250 0.003659 0.017285 0.021426 0.005738 0.003522 0.020276 0.018724 0.006585 0.003563
Mitochondria 0.011549 0.005010 0.002867 0.003205 0.004330 0.022540 0.001546 0.013214 0.007381 0.017527 0.002286 0.010254
Mitotic Spindle 0.002165 0.011113 0.152032 0.075028 0.001939 0.006361 0.037162 0.109203 0.002034 0.007720 0.101506 0.099096
None 0.000548 0.002059 0.000066 0.000185 0.000139 0.000329 0.000178 0.000477 0.000312 0.000824 0.000115 0.000391
Nuclear Periphery 0.000081 0.000098 0.002230 0.000497 0.000129 0.000178 0.001398 0.001633 0.000108 0.000155 0.001864 0.001297
Nuclear Periphery Foci 0.000991 0.003495 0.024396 0.003214 0.002843 0.002039 0.023364 0.001294 0.002060 0.002455 0.023942 0.001862
Nucleolus 0.000980 0.000615 0.007124 0.001350 0.000969 0.001674 0.015695 0.005874 0.000974 0.001371 0.010894 0.004536
Nucleus 0.002151 0.001322 0.004257 0.001448 0.001141 0.003197 0.007340 0.016574 0.001568 0.002661 0.005613 0.012101
Peroxisomes 0.053728 0.033362 0.032733 0.068586 0.032422 0.046031 0.030420 0.032379 0.041427 0.042408 0.031716 0.043087
Vacuole 0.022862 0.027337 0.113458 0.114586 0.019851 0.022779 0.108139 0.155935 0.021124 0.024082 0.111118 0.143707
Vacuole Periphery 0.002988 0.003579 0.009587 0.004477 0.002203 0.005808 0.007042 0.004190 0.002535 0.005171 0.008468 0.004275

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.18 -4.64 -4.01 -3.37 -0.51 -3.19 -3.28 -2.87 -0.14 -2.18 -3.61 -4.93 -4.85 -2.38 0.23
Bud Neck 5.13 4.60 1.92 -4.37 -3.83 -0.27 -5.04 -25.00 -23.16 -19.40 3.70 0.11 -20.05 -22.89 -21.57
Bud Site -3.23 -10.78 -13.35 -10.08 -8.56 -3.94 -7.34 -16.53 -8.80 -7.88 -4.85 -13.17 -20.91 -13.15 -10.49
Cell Periphery 3.16 2.28 2.60 -1.63 1.28 -1.20 0.86 1.70 1.24 1.56 -1.02 1.99 3.38 1.21 4.81
Cytoplasm 0.06 6.65 -8.17 -6.43 -12.12 -4.57 0.78 -27.43 -23.43 -27.41 -2.30 5.74 -27.10 -23.32 -30.13
Cytoplasmic Foci 6.05 2.34 1.36 -5.00 -0.99 -11.08 3.70 -4.04 8.75 -7.56 -4.02 1.27 0.56 4.66 -0.86
Eisosomes 5.52 5.14 8.14 1.64 5.14 -5.13 -3.94 2.97 6.67 7.17 -1.48 1.23 8.75 6.83 9.70
Endoplasmic Reticulum -0.36 -7.36 -3.05 -2.75 4.29 -0.76 -4.73 -1.75 -0.66 4.19 -1.04 -8.28 -3.41 -1.69 6.44
Endosome -6.31 19.79 20.23 21.21 1.24 15.32 14.60 61.71 31.71 27.09 7.32 26.50 58.22 37.64 20.28
Golgi 4.69 5.09 10.43 3.17 6.71 -11.85 10.29 8.52 16.29 -2.09 -7.46 7.14 14.27 16.02 7.71
Lipid Particles 4.05 7.95 9.70 3.28 4.07 -1.35 6.73 8.81 7.14 2.75 0.77 10.25 13.13 7.83 5.25
Mitochondria 2.68 5.84 5.34 0.99 -0.01 -6.66 4.41 -10.52 3.50 -17.69 -4.79 6.91 -3.10 3.66 -14.11
Mitotic Spindle -3.24 -19.98 -14.63 -11.73 7.76 -3.96 -8.76 -26.53 -24.67 -10.58 -5.05 -21.09 -30.23 -27.16 0.84
None -0.74 2.56 1.44 0.90 -1.92 -1.11 -0.54 -5.05 -3.16 -6.33 -0.82 2.01 -2.00 0.48 -8.30
Nuclear Periphery -0.75 -11.69 -6.19 -5.95 6.91 -1.13 -7.73 -11.93 -11.69 -3.78 -1.64 -13.90 -13.05 -12.71 -0.21
Nuclear Periphery Foci -1.46 -10.33 -6.41 -0.72 8.53 1.52 -7.15 2.39 0.97 7.66 -0.42 -12.04 -0.85 -0.14 11.93
Nucleolus 1.52 -5.40 -3.00 -3.67 4.56 -1.03 -7.58 -12.75 -9.97 3.84 -0.72 -9.25 -13.15 -10.39 4.13
Nucleus 0.91 -3.28 -1.40 -2.64 2.40 -2.00 -5.70 -15.82 -12.41 -7.12 -1.42 -6.76 -15.33 -12.51 -8.21
Peroxisomes 4.73 6.19 -1.70 -5.70 -6.94 -4.27 0.84 1.97 5.99 0.74 -0.35 4.63 1.71 1.75 -3.06
Vacuole -1.37 -19.24 -20.07 -17.22 -3.76 -1.25 -15.84 -38.17 -35.85 -9.29 -1.64 -25.10 -42.88 -39.26 -10.45
Vacuole Periphery -0.86 -9.50 -1.90 -0.58 7.05 -4.98 -7.20 -4.98 3.03 5.17 -4.79 -12.20 -4.82 2.52 9.66
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy
Localization
Cell Percentages endosome (60%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ccz1

Ccz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available