ORF
Human Ortholog
Description Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.96 – 0.85 0.86 0.82 0.79 0.71 0.59 0.59 0.41 0.41 0.92 0.96 0.96 0.82 0.82 0.85 0.91 0.82 0.72 0.75 0.69 0.63
Endoplasmic Reticulum – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.06 0 0 0.08
Golgi – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 – 0.1 0 0.22 0.05 0.11 0.46 0.38 0.5 0.5 0 0 0 0 0 0 0 0 0.05 0.08 0.1 0.09
Nucleus – 0 – 0 0 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 – 0.1 0.16 0 0.17 0.18 0.11 0.14 0.15 0.25 0.06 0.05 0.05 0.17 0.13 0.1 0 0.05 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 4 9 15
Bud – 0 – 0 0 1 1 0 1 1 1 1 0 0 0 0 0 0 0 1 0 6 13 13
Bud Neck – 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 2 2
Bud Site – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – – – – – –
Cell Periphery – 0 – 0 0 0 0 3 1 4 0 0 4 0 1 3 5 6 0 0 0 0 0 1
Cytoplasm – 319 – 17 124 37 84 145 64 53 14 18 449 640 742 159 111 133 10 300 44 391 462 387
Endoplasmic Reticulum – 4 – 0 0 1 1 0 0 0 0 0 11 8 1 6 3 6 0 5 1 12 10 7
Endosome – 1 – 0 5 0 3 4 0 0 0 0 12 10 7 12 4 6 0 6 3 13 27 46
Golgi – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 8 6
Mitochondria – 6 – 2 0 10 5 22 50 34 17 22 1 3 1 6 2 4 0 14 2 44 69 53
Nucleus – 2 – 0 1 4 4 6 2 2 1 2 0 0 0 3 2 3 0 2 1 4 3 5
Nuclear Periphery – 0 – 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0
Nucleolus – 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 2
Peroxisomes – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
SpindlePole – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 2 7 14 22
Vac/Vac Membrane – 10 – 2 23 1 18 36 12 13 5 11 31 34 38 33 18 16 0 18 1 21 30 32
Unique Cell Count 333 20 145 45 107 203 109 90 34 44 487 669 770 194 136 156 12 367 61 525 668 611
Labelled Cell Count 343 21 153 55 116 216 131 107 38 54 508 696 790 225 150 175 12 367 61 525 668 611


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.1 – 5.5 7.1 5.0 7.1 7.2 5.8 6.5 5.8 6.0 5.9 6.2 6.9 6.8 6.8 6.8 4.7 4.7 5.0
Std Deviation (1e-4) – 0.8 – 1.8 1.6 1.7 2.6 2.1 1.1 1.8 1.5 1.2 1.1 1.1 1.2 1.4 1.3 1.6 1.4 1.2 1.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9101 2.2024 2.1673 1.9643 1.8748 1.8756 2.7859 2.9515 2.6609 2.5671 2.6729 2.9788 5.0338 5.7551 5.4785 5.1064 4.2567 5.1872
Actin 0.0204 0.0032 0.0214 0.0006 0.0117 0.0006 0.0235 0.0006 0.0079 0.0064 0.0044 0.002 0.1513 0.0019 0.0109 0.0049 0.0031 0.0114
Bud 0.001 0.0022 0.0016 0.0003 0.0008 0.0002 0.0008 0.0008 0.0052 0.0005 0.0005 0.0016 0.0039 0.0019 0.002 0.0003 0.0003 0.0004
Bud Neck 0.0052 0.0006 0.0017 0.0023 0.0011 0.0023 0.0016 0.0007 0.0017 0.0008 0.0047 0.002 0.0125 0.0004 0.0005 0.0005 0.0007 0.0015
Bud Periphery 0.0013 0.0016 0.0014 0.0001 0.0012 0.0001 0.0009 0.0005 0.0021 0.0003 0.0006 0.0016 0.0033 0.0005 0.0018 0.0001 0.0003 0.0006
Bud Site 0.0048 0.0068 0.007 0.0003 0.0017 0.0004 0.0062 0.0075 0.006 0.0011 0.0071 0.0005 0.0132 0.0138 0.0106 0.0007 0.0003 0.0003
Cell Periphery 0.0002 0.0002 0.0002 0 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.001 0.0003 0.0005 0.0001 0.0001 0.0001
Cytoplasm 0.5553 0.7219 0.6567 0.7022 0.5349 0.7278 0.5811 0.6758 0.6699 0.6895 0.6812 0.7141 0.4025 0.6888 0.7216 0.6602 0.6613 0.7123
Cytoplasmic Foci 0.0403 0.0202 0.0202 0.0131 0.0424 0.0378 0.0318 0.0206 0.0228 0.0207 0.0545 0.0336 0.0294 0.0164 0.0151 0.0253 0.027 0.0153
Eisosomes 0.0002 0.0001 0.0002 0 0.0002 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0015 0.0001 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0123 0.0059 0.0096 0.006 0.01 0.006 0.007 0.0052 0.0047 0.0068 0.0064 0.0053 0.008 0.0078 0.0118 0.0222 0.0269 0.0085
Endosome 0.0358 0.0156 0.0211 0.0091 0.0883 0.0231 0.0481 0.0149 0.0157 0.0332 0.0881 0.029 0.0226 0.0137 0.0184 0.0374 0.0386 0.0231
Golgi 0.0072 0.0015 0.0027 0.0003 0.0204 0.0009 0.0092 0.001 0.0025 0.0021 0.0163 0.0028 0.0151 0.0006 0.0009 0.0014 0.0027 0.0016
Lipid Particles 0.0105 0.0005 0.003 0.0001 0.03 0.0008 0.0089 0.0006 0.003 0.0051 0.0159 0.0067 0.0212 0.0005 0.0006 0.003 0.0074 0.0007
Mitochondria 0.0026 0.0008 0.0057 0.0006 0.0196 0.0008 0.0054 0.0008 0.0067 0.0014 0.0061 0.0041 0.025 0.0005 0.0011 0.0012 0.001 0.0015
None 0.187 0.1522 0.1295 0.1795 0.1107 0.0936 0.159 0.195 0.173 0.1308 0.0417 0.1008 0.1004 0.1938 0.1251 0.1554 0.1304 0.1228
Nuclear Periphery 0.0123 0.0063 0.0139 0.011 0.035 0.0059 0.0222 0.0059 0.0093 0.0068 0.0117 0.0059 0.0437 0.0076 0.0081 0.0237 0.012 0.0082
Nucleolus 0.0016 0.0005 0.0021 0.0005 0.0025 0.001 0.0016 0.0012 0.0007 0.0005 0.0017 0.0008 0.0014 0.0012 0.0005 0.0005 0.0003 0.0003
Nucleus 0.0776 0.0467 0.0816 0.0504 0.0517 0.079 0.0576 0.0517 0.0409 0.063 0.0311 0.0662 0.1037 0.0377 0.0497 0.0465 0.0784 0.075
Peroxisomes 0.01 0.0012 0.0024 0.0003 0.0113 0.0014 0.0085 0.001 0.0129 0.0146 0.0025 0.0043 0.024 0.0003 0.0004 0.0008 0.0006 0.0005
Punctate Nuclear 0.0085 0.0064 0.0115 0.019 0.0104 0.0137 0.0152 0.0092 0.0105 0.0089 0.0165 0.014 0.0097 0.0092 0.0172 0.0094 0.0042 0.0118
Vacuole 0.0049 0.0049 0.0052 0.0034 0.011 0.0038 0.0084 0.006 0.0037 0.0059 0.0063 0.0036 0.0046 0.0027 0.0024 0.005 0.0033 0.0032
Vacuole Periphery 0.001 0.0006 0.0013 0.0007 0.005 0.0008 0.0024 0.0007 0.0008 0.0014 0.0023 0.0009 0.002 0.0005 0.0006 0.0013 0.0011 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.6718 45.6656 47.4401 36.1645 42.3885 57.1819 42.4786 41.7588 51.4036 35.5234
Translational Efficiency 2.0865 1.3574 1.3136 1.9884 1.2576 1.5029 1.6856 1.8161 1.758 1.7592

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1615 890 2219 132 1768 2515 1505 1482 3383 3405 3724 1614

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.22 811.31 854.59 992.87 679.75 807.57 766.32 903.17 689.52 808.55 818.92 910.51
Standard Deviation 78.74 108.97 109.96 129.41 75.11 96.40 113.29 119.15 77.54 99.85 119.45 122.52
Intensity Change Log 2 — 0.212445 0.287424 0.503797 — 0.248583 0.172943 0.409993 — 0.230359 0.232167 0.458352

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000194 0.000504 0.000214 0.000585 0.000047 0.000162 0.000195 0.000725 0.000117 0.000251 0.000206 0.000713
Bud Neck 0.014272 0.013213 0.004990 0.002933 0.010414 0.021943 0.002508 0.009142 0.012256 0.019661 0.003987 0.008634
Bud Site 0.006001 0.012722 0.006630 0.007256 0.002187 0.012496 0.005910 0.025494 0.004008 0.012555 0.006339 0.024002
Cell Periphery 0.000146 0.000141 0.000096 0.000208 0.000190 0.000106 0.000210 0.000133 0.000169 0.000115 0.000142 0.000139
Cytoplasm 0.478409* 0.498228* 0.423263* 0.357054* 0.501641* 0.512891* 0.531631* 0.532020* 0.490550* 0.509058* 0.467058* 0.517711*
Cytoplasmic Foci 0.241769* 0.219835* 0.007628 0.044705 0.171931 0.145932 0.017564 0.019118 0.205271* 0.165249 0.011644 0.021211
Eisosomes 0.000146 0.000087 0.000015 0.000106 0.000130 0.000066 0.000040 0.000031 0.000138 0.000071 0.000025 0.000038
Endoplasmic Reticulum 0.001934 0.002583 0.003060 0.005913 0.002158 0.001577 0.002165 0.003136 0.002051 0.001840 0.002698 0.003363
Endosome 0.009291 0.016975 0.000982 0.009445 0.007997 0.012764 0.001866 0.004440 0.008614 0.013865 0.001339 0.004850
Golgi 0.003172 0.002692 0.000063 0.000145 0.001530 0.002446 0.000107 0.003045 0.002314 0.002510 0.000080 0.002808
Lipid Particles 0.011376 0.006451 0.000599 0.004902 0.012444 0.004694 0.001373 0.001453 0.011934 0.005153 0.000912 0.001735
Mitochondria 0.007403 0.003628 0.000801 0.000298 0.003545 0.007092 0.000463 0.004672 0.005387 0.006187 0.000665 0.004314
Mitotic Spindle 0.003222 0.003595 0.004444 0.072785 0.001169 0.010171 0.005066 0.047926 0.002149 0.008452 0.004695 0.049959
None 0.002504 0.002989 0.004789 0.004247 0.006584 0.004789 0.014122 0.007525 0.004636 0.004319 0.008561 0.007257
Nuclear Periphery 0.000571 0.000691 0.002408 0.002131 0.000938 0.001014 0.000953 0.002496 0.000763 0.000930 0.001820 0.002466
Nuclear Periphery Foci 0.001214 0.001632 0.001118 0.009861 0.000268 0.000414 0.002439 0.002996 0.000720 0.000733 0.001652 0.003557
Nucleolus 0.001320 0.001359 0.000324 0.000456 0.001269 0.001597 0.000769 0.000978 0.001293 0.001535 0.000504 0.000935
Nucleus 0.172199* 0.131680* 0.512756* 0.203226* 0.233993* 0.192628* 0.369794* 0.257673* 0.204493* 0.176697* 0.454980* 0.253220*
Peroxisomes 0.005197 0.007694 0.000181 0.000910 0.002192 0.004548 0.000269 0.000740 0.003626 0.005370 0.000216 0.000754
Vacuole 0.038414 0.071368 0.025517 0.272664* 0.038051 0.060733 0.042331 0.073616 0.038224 0.063513 0.032312 0.089895
Vacuole Periphery 0.001246 0.001932 0.000124 0.000168 0.001322 0.001938 0.000225 0.002641 0.001286 0.001937 0.000165 0.002439

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.48 -1.58 -5.00 -2.46 -4.53 -5.35 -9.34 -5.18 -3.94 -2.74 -3.39 -5.27 -5.14 -3.42 -3.36
Bud Neck 0.74 7.12 4.92 3.63 -1.07 -7.12 6.50 0.74 8.57 -7.06 -5.94 9.16 2.83 8.49 -5.71
Bud Site -3.35 -0.22 -1.66 -0.00 -1.59 -8.32 -2.70 -6.92 -1.52 -4.50 -7.69 -2.19 -6.18 -1.54 -4.94
Cell Periphery 0.26 4.31 -2.99 -3.17 -5.56 6.88 -0.06 6.61 -0.26 6.07 6.26 3.38 5.82 -0.09 2.65
Cytoplasm -1.36 10.09 12.87 12.94 8.83 -1.19 0.82 7.53 8.88 6.05 -2.54 9.07 9.36 11.13 2.32
Cytoplasmic Foci 2.72 43.49 28.89 22.23 -3.97 4.69 35.44 34.62 34.24 -1.40 8.60 55.68 51.10 42.60 -6.36
Eisosomes 7.45 19.10 1.36 -4.71 -13.47 5.64 7.73 7.86 5.93 0.25 9.39 16.38 12.93 6.43 -9.98
Endoplasmic Reticulum -1.52 -4.94 -6.89 -6.47 -5.16 1.16 -2.20 -3.91 -6.86 -2.81 0.47 -5.35 -6.55 -8.39 -2.53
Endosome -5.34 12.61 0.19 4.39 -6.24 -5.00 11.19 7.24 10.72 -4.53 -6.60 17.15 8.91 13.19 -7.79
Golgi 1.02 7.97 7.48 6.44 -5.30 -2.55 6.77 0.43 1.83 -2.12 -0.50 10.31 2.01 2.28 -2.19
Lipid Particles 5.43 13.39 6.20 1.68 -7.43 8.93 13.12 12.80 10.65 -1.80 11.01 18.86 16.90 12.63 -8.12
Mitochondria 2.97 5.63 5.82 5.03 1.84 -4.46 6.60 1.09 4.42 -2.57 -0.95 7.72 3.17 4.25 -2.29
Mitotic Spindle -0.22 -1.34 -3.44 -3.42 -3.32 -5.66 -3.51 -10.38 -7.75 -9.01 -4.97 -3.50 -10.71 -8.87 -9.89
None -0.99 -6.34 -0.72 -0.19 1.85 1.73 -3.67 -0.25 -2.25 3.72 0.58 -4.20 -2.37 -2.85 1.11
Nuclear Periphery -2.15 -14.63 -9.01 -8.60 -2.91 -0.52 -3.42 -14.14 -12.47 -12.65 -1.50 -13.56 -16.92 -14.56 -8.80
Nuclear Periphery Foci -0.82 -0.76 -3.65 -3.57 -3.58 -1.38 -5.93 -8.15 -7.47 -0.75 -0.19 -4.56 -6.83 -7.30 -4.47
Nucleolus 0.02 4.67 0.67 0.68 -2.10 -1.52 3.52 -0.96 -0.06 -2.87 -1.25 6.09 -0.78 -0.12 -3.61
Nucleus 5.58 -48.02 -2.26 -4.26 15.78 6.39 -17.09 -7.84 -13.78 8.92 5.74 -45.57 -12.32 -16.29 23.07
Peroxisomes -1.82 9.88 7.06 5.26 -2.62 -4.84 7.12 4.68 8.82 -2.70 -3.29 12.09 9.05 9.98 -3.33
Vacuole -9.04 -0.56 -14.79 -13.13 -14.72 -8.44 -7.45 -19.92 -15.26 -13.64 -12.09 -5.66 -23.20 -17.79 -20.64
Vacuole Periphery -2.55 9.20 8.56 7.68 -3.90 -2.75 7.18 0.38 1.64 -2.08 -3.85 11.40 0.51 1.96 -2.21
ORF
Human Ortholog
Description Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene
Localization
Cell Percentages cytoplasm (33%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBR137W

YBR137W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr137w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available