ORF | |
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Human Ortholog | |
Description | Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 4 | 9 | 15 |
Bud | – | 0 | – | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 13 | 13 |
Bud Neck | – | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 2 |
Bud Site | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | – | 0 | – | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 0 | 0 | 4 | 0 | 1 | 3 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | – | 319 | – | 17 | 124 | 37 | 84 | 145 | 64 | 53 | 14 | 18 | 449 | 640 | 742 | 159 | 111 | 133 | 10 | 300 | 44 | 391 | 462 | 387 |
Endoplasmic Reticulum | – | 4 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 11 | 8 | 1 | 6 | 3 | 6 | 0 | 5 | 1 | 12 | 10 | 7 |
Endosome | – | 1 | – | 0 | 5 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 12 | 10 | 7 | 12 | 4 | 6 | 0 | 6 | 3 | 13 | 27 | 46 |
Golgi | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 6 |
Mitochondria | – | 6 | – | 2 | 0 | 10 | 5 | 22 | 50 | 34 | 17 | 22 | 1 | 3 | 1 | 6 | 2 | 4 | 0 | 14 | 2 | 44 | 69 | 53 |
Nucleus | – | 2 | – | 0 | 1 | 4 | 4 | 6 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 3 | 2 | 3 | 0 | 2 | 1 | 4 | 3 | 5 |
Nuclear Periphery | – | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 |
Nucleolus | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Peroxisomes | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
SpindlePole | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 7 | 14 | 22 |
Vac/Vac Membrane | – | 10 | – | 2 | 23 | 1 | 18 | 36 | 12 | 13 | 5 | 11 | 31 | 34 | 38 | 33 | 18 | 16 | 0 | 18 | 1 | 21 | 30 | 32 |
Unique Cell Count | 333 | 20 | 145 | 45 | 107 | 203 | 109 | 90 | 34 | 44 | 487 | 669 | 770 | 194 | 136 | 156 | 12 | 367 | 61 | 525 | 668 | 611 | ||
Labelled Cell Count | 343 | 21 | 153 | 55 | 116 | 216 | 131 | 107 | 38 | 54 | 508 | 696 | 790 | 225 | 150 | 175 | 12 | 367 | 61 | 525 | 668 | 611 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 5.1 | – | 5.5 | 7.1 | 5.0 | 7.1 | 7.2 | 5.8 | 6.5 | 5.8 | 6.0 | 5.9 | 6.2 | 6.9 | 6.8 | 6.8 | 6.8 | 4.7 | 4.7 | 5.0 |
Std Deviation (1e-4) | – | 0.8 | – | 1.8 | 1.6 | 1.7 | 2.6 | 2.1 | 1.1 | 1.8 | 1.5 | 1.2 | 1.1 | 1.1 | 1.2 | 1.4 | 1.3 | 1.6 | 1.4 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9101 | 2.2024 | 2.1673 | 1.9643 | 1.8748 | 1.8756 | 2.7859 | 2.9515 | 2.6609 | 2.5671 | 2.6729 | 2.9788 | 5.0338 | 5.7551 | 5.4785 | 5.1064 | 4.2567 | 5.1872 |
Actin | 0.0204 | 0.0032 | 0.0214 | 0.0006 | 0.0117 | 0.0006 | 0.0235 | 0.0006 | 0.0079 | 0.0064 | 0.0044 | 0.002 | 0.1513 | 0.0019 | 0.0109 | 0.0049 | 0.0031 | 0.0114 |
Bud | 0.001 | 0.0022 | 0.0016 | 0.0003 | 0.0008 | 0.0002 | 0.0008 | 0.0008 | 0.0052 | 0.0005 | 0.0005 | 0.0016 | 0.0039 | 0.0019 | 0.002 | 0.0003 | 0.0003 | 0.0004 |
Bud Neck | 0.0052 | 0.0006 | 0.0017 | 0.0023 | 0.0011 | 0.0023 | 0.0016 | 0.0007 | 0.0017 | 0.0008 | 0.0047 | 0.002 | 0.0125 | 0.0004 | 0.0005 | 0.0005 | 0.0007 | 0.0015 |
Bud Periphery | 0.0013 | 0.0016 | 0.0014 | 0.0001 | 0.0012 | 0.0001 | 0.0009 | 0.0005 | 0.0021 | 0.0003 | 0.0006 | 0.0016 | 0.0033 | 0.0005 | 0.0018 | 0.0001 | 0.0003 | 0.0006 |
Bud Site | 0.0048 | 0.0068 | 0.007 | 0.0003 | 0.0017 | 0.0004 | 0.0062 | 0.0075 | 0.006 | 0.0011 | 0.0071 | 0.0005 | 0.0132 | 0.0138 | 0.0106 | 0.0007 | 0.0003 | 0.0003 |
Cell Periphery | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.5553 | 0.7219 | 0.6567 | 0.7022 | 0.5349 | 0.7278 | 0.5811 | 0.6758 | 0.6699 | 0.6895 | 0.6812 | 0.7141 | 0.4025 | 0.6888 | 0.7216 | 0.6602 | 0.6613 | 0.7123 |
Cytoplasmic Foci | 0.0403 | 0.0202 | 0.0202 | 0.0131 | 0.0424 | 0.0378 | 0.0318 | 0.0206 | 0.0228 | 0.0207 | 0.0545 | 0.0336 | 0.0294 | 0.0164 | 0.0151 | 0.0253 | 0.027 | 0.0153 |
Eisosomes | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0015 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0123 | 0.0059 | 0.0096 | 0.006 | 0.01 | 0.006 | 0.007 | 0.0052 | 0.0047 | 0.0068 | 0.0064 | 0.0053 | 0.008 | 0.0078 | 0.0118 | 0.0222 | 0.0269 | 0.0085 |
Endosome | 0.0358 | 0.0156 | 0.0211 | 0.0091 | 0.0883 | 0.0231 | 0.0481 | 0.0149 | 0.0157 | 0.0332 | 0.0881 | 0.029 | 0.0226 | 0.0137 | 0.0184 | 0.0374 | 0.0386 | 0.0231 |
Golgi | 0.0072 | 0.0015 | 0.0027 | 0.0003 | 0.0204 | 0.0009 | 0.0092 | 0.001 | 0.0025 | 0.0021 | 0.0163 | 0.0028 | 0.0151 | 0.0006 | 0.0009 | 0.0014 | 0.0027 | 0.0016 |
Lipid Particles | 0.0105 | 0.0005 | 0.003 | 0.0001 | 0.03 | 0.0008 | 0.0089 | 0.0006 | 0.003 | 0.0051 | 0.0159 | 0.0067 | 0.0212 | 0.0005 | 0.0006 | 0.003 | 0.0074 | 0.0007 |
Mitochondria | 0.0026 | 0.0008 | 0.0057 | 0.0006 | 0.0196 | 0.0008 | 0.0054 | 0.0008 | 0.0067 | 0.0014 | 0.0061 | 0.0041 | 0.025 | 0.0005 | 0.0011 | 0.0012 | 0.001 | 0.0015 |
None | 0.187 | 0.1522 | 0.1295 | 0.1795 | 0.1107 | 0.0936 | 0.159 | 0.195 | 0.173 | 0.1308 | 0.0417 | 0.1008 | 0.1004 | 0.1938 | 0.1251 | 0.1554 | 0.1304 | 0.1228 |
Nuclear Periphery | 0.0123 | 0.0063 | 0.0139 | 0.011 | 0.035 | 0.0059 | 0.0222 | 0.0059 | 0.0093 | 0.0068 | 0.0117 | 0.0059 | 0.0437 | 0.0076 | 0.0081 | 0.0237 | 0.012 | 0.0082 |
Nucleolus | 0.0016 | 0.0005 | 0.0021 | 0.0005 | 0.0025 | 0.001 | 0.0016 | 0.0012 | 0.0007 | 0.0005 | 0.0017 | 0.0008 | 0.0014 | 0.0012 | 0.0005 | 0.0005 | 0.0003 | 0.0003 |
Nucleus | 0.0776 | 0.0467 | 0.0816 | 0.0504 | 0.0517 | 0.079 | 0.0576 | 0.0517 | 0.0409 | 0.063 | 0.0311 | 0.0662 | 0.1037 | 0.0377 | 0.0497 | 0.0465 | 0.0784 | 0.075 |
Peroxisomes | 0.01 | 0.0012 | 0.0024 | 0.0003 | 0.0113 | 0.0014 | 0.0085 | 0.001 | 0.0129 | 0.0146 | 0.0025 | 0.0043 | 0.024 | 0.0003 | 0.0004 | 0.0008 | 0.0006 | 0.0005 |
Punctate Nuclear | 0.0085 | 0.0064 | 0.0115 | 0.019 | 0.0104 | 0.0137 | 0.0152 | 0.0092 | 0.0105 | 0.0089 | 0.0165 | 0.014 | 0.0097 | 0.0092 | 0.0172 | 0.0094 | 0.0042 | 0.0118 |
Vacuole | 0.0049 | 0.0049 | 0.0052 | 0.0034 | 0.011 | 0.0038 | 0.0084 | 0.006 | 0.0037 | 0.0059 | 0.0063 | 0.0036 | 0.0046 | 0.0027 | 0.0024 | 0.005 | 0.0033 | 0.0032 |
Vacuole Periphery | 0.001 | 0.0006 | 0.0013 | 0.0007 | 0.005 | 0.0008 | 0.0024 | 0.0007 | 0.0008 | 0.0014 | 0.0023 | 0.0009 | 0.002 | 0.0005 | 0.0006 | 0.0013 | 0.0011 | 0.0008 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.6718 | 45.6656 | 47.4401 | 36.1645 | 42.3885 | 57.1819 | 42.4786 | 41.7588 | 51.4036 | 35.5234 |
Translational Efficiency | 2.0865 | 1.3574 | 1.3136 | 1.9884 | 1.2576 | 1.5029 | 1.6856 | 1.8161 | 1.758 | 1.7592 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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