ORF
Human Ortholog
Description Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.82 0.93 0.94 0.95 0.89 0.8 0.79 0.76 0.66 0.68 0.62 0.56 0.99 0.99 0.98 – – 0.85 0.72 0.88 0.6 0.73 0.78 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.35 0.19 0.08 0.08 0.11 0.4 0.32 0.35 0.51 0.53 0.51 0.63 0 0 0 – – 0.07 0.17 0.06 0.19 0.07 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 4 7 3 5
Bud 1 3 1 1 2 6 8 12 8 6 7 3 1 0 0 – – 0 0 0 5 4 8 13
Bud Neck 1 1 1 0 3 3 2 13 4 3 2 2 0 0 0 – – 2 0 0 3 0 1 8
Bud Site 0 0 0 0 0 1 0 0 1 1 3 1 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 2 0 3 0 3 0 0 0 0 – – 4 0 0 2 1 1 2
Cytoplasm 69 110 207 189 215 247 320 352 185 246 121 90 155 281 307 – – 81 69 106 133 104 235 203
Endoplasmic Reticulum 1 0 0 1 0 0 0 0 2 0 2 0 0 0 2 – – 8 1 0 2 1 3 3
Endosome 0 0 0 1 3 1 0 0 0 0 1 0 0 1 0 – – 5 0 0 4 1 5 2
Golgi 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 – – 0 0 0 0 0 1 1
Mitochondria 29 23 18 16 27 122 129 162 144 191 99 101 3 3 3 – – 7 16 7 41 10 21 18
Nucleus 0 0 1 0 0 0 2 0 0 1 3 3 0 0 0 – – 0 0 0 1 0 1 0
Nuclear Periphery 0 0 2 0 0 0 0 0 0 1 0 1 0 0 1 – – 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – – 1 0 0 0 1 0 0
SpindlePole 0 0 1 3 3 3 11 12 11 5 6 7 0 0 1 – – 0 1 0 8 2 5 7
Vac/Vac Membrane 0 0 0 0 3 0 1 2 1 0 0 1 0 0 0 – – 6 3 1 4 1 3 10
Unique Cell Count 84 118 220 198 241 307 404 462 282 360 195 160 157 283 312 95 97 121 222 142 302 288
Labelled Cell Count 101 137 231 211 257 385 476 555 359 455 247 209 159 285 315 114 97 121 222 142 302 288


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 3.1 3.7 3.4 3.5 3.0 2.7 2.9 2.6 2.5 2.5 2.7 3.9 3.8 4.1 4.9 4.7 5.1 4.2 4.3 5.2
Std Deviation (1e-4) 0.6 0.7 1.7 1.1 1.5 1.6 1.4 1.3 1.1 1.1 1.1 1.4 1.2 1.0 1.7 2.3 1.8 1.9 1.8 2.3 3.0
Intensity Change (Log2) – – – -0.12 -0.07 -0.29 -0.45 -0.36 -0.49 -0.59 -0.56 -0.46 0.06 0.03 0.13 0.39 0.33 0.47 0.18 0.2 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7469 -0.7048 -0.757 -0.7808 -0.8344 -0.7867 -0.791 -0.6527 -0.713 -0.7056 -1.0568 -0.6762 0.391 0.4477 0.3443 0.4075 0.2522 0.4314
Actin 0.0625 0 0.0121 0.0002 0.0017 0.012 0.0301 0.0004 0.002 0.0005 0.0012 0.0002 0.0292 0.0001 0.0343 0.0521 0.0051 0.0107
Bud 0.0007 0 0.0003 0 0.0003 0 0.0007 0.0002 0.0006 0.0001 0.0001 0 0.0007 0.0001 0.001 0.0016 0.0001 0.0007
Bud Neck 0.0155 0 0.0003 0.0001 0.0008 0.0046 0.0065 0 0.0045 0 0.001 0 0.0006 0 0.0079 0.0001 0.0001 0.0004
Bud Periphery 0.0016 0 0.0009 0 0.001 0.0001 0.0011 0.0006 0.0027 0.0002 0.0001 0.0001 0.0011 0.0001 0.003 0.0031 0.0002 0.0044
Bud Site 0.0043 0 0.0012 0 0.0007 0 0.0289 0.0002 0.0005 0.0001 0.0005 0 0.0058 0.0005 0.0102 0.0014 0.0003 0.0003
Cell Periphery 0.0006 0.0001 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0004 0.0001 0.0005 0 0 0.0001
Cytoplasm 0.0163 0.0185 0.0183 0.0084 0.0116 0.0311 0.0157 0.0118 0.0096 0.0137 0.0081 0.018 0.0182 0.0271 0.0342 0.0138 0.0143 0.0468
Cytoplasmic Foci 0.0229 0.0017 0.0081 0.0028 0.0147 0.012 0.0325 0.0044 0.0085 0.0042 0.0112 0.0031 0.0158 0.0025 0.0101 0.0173 0.0056 0.0087
Eisosomes 0.0015 0.0002 0.0004 0.0004 0.0003 0.0002 0.0009 0.0003 0.0008 0.0009 0.0001 0.0002 0.0005 0.0002 0.0004 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0024 0.0011 0.001 0.0003 0.0002 0.0002 0.0035 0.0002 0.0012 0.0006 0.0001 0.0002 0.0042 0.0003 0.0011 0.0003 0.0004 0.0012
Endosome 0.0135 0.0005 0.0033 0.0001 0.0011 0.0006 0.0491 0.0004 0.0082 0.0025 0.0057 0.0026 0.0205 0.0001 0.0055 0.0009 0.0057 0.0061
Golgi 0.0077 0 0.0048 0 0.0013 0.0003 0.0162 0.0001 0.0034 0.0015 0.004 0.0006 0.0041 0 0.0032 0.0028 0.0037 0.0028
Lipid Particles 0.0144 0.0006 0.0037 0.0004 0.0073 0.0003 0.0201 0.0008 0.0154 0.0012 0.0072 0.0005 0.025 0.0002 0.0046 0.0012 0.0092 0.003
Mitochondria 0.0052 0.0001 0.0028 0.0002 0.081 0.0001 0.0086 0.0008 0.0018 0.0377 0.0116 0.0075 0.0028 0.0001 0.0033 0.0028 0.0014 0.0019
None 0.791 0.9756 0.9325 0.9859 0.8358 0.9374 0.7347 0.9771 0.9204 0.934 0.9073 0.9657 0.828 0.9675 0.8637 0.8643 0.9382 0.908
Nuclear Periphery 0.0084 0.0004 0.0007 0.0001 0.0001 0.0001 0.0049 0.0001 0.0119 0.0003 0 0.0001 0.0077 0.0001 0.0045 0.0002 0.0063 0.0005
Nucleolus 0.0047 0.0001 0.0003 0.0001 0.0007 0.0001 0.0016 0.0002 0.0008 0.0001 0.0004 0 0.0014 0.0001 0.0004 0.0001 0.0006 0.0001
Nucleus 0.0015 0.0003 0.0005 0.0001 0.0003 0.0001 0.0024 0.0002 0.0008 0.0002 0.0001 0.0001 0.0013 0.0002 0.001 0.0001 0.0009 0.0001
Peroxisomes 0.0161 0 0.0025 0.0002 0.0392 0.0002 0.0275 0.0013 0.0026 0.0006 0.0393 0.0001 0.0188 0.0001 0.0088 0.0357 0.0013 0.0032
Punctate Nuclear 0.0068 0.0003 0.0058 0.0003 0.0004 0.0007 0.0093 0.0003 0.0005 0.0002 0.0002 0.0001 0.0058 0.0002 0.0014 0.0018 0.0056 0.0002
Vacuole 0.0014 0.0004 0.0002 0.0001 0.0009 0.0001 0.0034 0.0003 0.0025 0.0007 0.0013 0.0004 0.0065 0.0002 0.0007 0.0001 0.0003 0.0006
Vacuole Periphery 0.0009 0 0.0001 0 0.0003 0 0.0017 0.0001 0.001 0.0005 0.0003 0.0005 0.0018 0 0.0003 0 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.8532 10.0203 9.2944 8.9061 2.725 7.0019 12.5061 17.5227 10.3737 6.5181
Translational Efficiency 1.1511 1.7471 2.0423 1.0902 1.7599 0.5564 1.6333 1.3158 1.5122 1.3139

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1376 1259 159 1528 1292 1884 149 649 2668 3143 308 2177

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 741.27 815.67 1415.01 1100.18 895.84 951.00 1348.42 1192.64 816.12 896.79 1382.80 1127.74
Standard Deviation 98.73 181.56 181.12 241.02 129.49 161.24 171.44 203.35 138.26 182.17 179.61 234.28
Intensity Change Log 2 — 0.137986 0.932741 0.569669 — 0.086204 0.589957 0.412846 — 0.109881 0.755309 0.485972

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000248 0.000824 0.001423 0.000610 0.000587 0.000530 0.001248 0.001038 0.000412 0.000648 0.001338 0.000738
Bud Neck 0.003082 0.007511 0.012200 0.021811 0.004489 0.009654 0.011176 0.019542 0.003763 0.008796 0.011705 0.021135
Bud Site 0.000753 0.003028 0.001293 0.003144 0.000630 0.001799 0.001176 0.003696 0.000693 0.002291 0.001237 0.003309
Cell Periphery 0.000120 0.000305 0.000042 0.001912 0.000061 0.000164 0.000044 0.000114 0.000091 0.000221 0.000043 0.001376
Cytoplasm 0.001427 0.006135 0.000124 0.039653 0.000887 0.000568 0.000099 0.003227 0.001166 0.002798 0.000112 0.028794
Cytoplasmic Foci 0.000249 0.000779 0.000061 0.009800 0.000655 0.000526 0.000036 0.002422 0.000445 0.000627 0.000049 0.007600
Eisosomes 0.000031 0.000040 0.000099 0.001963 0.000039 0.000051 0.000084 0.000047 0.000035 0.000047 0.000092 0.001392
Endoplasmic Reticulum 0.002637 0.003990 0.002532 0.001580 0.003424 0.003788 0.002490 0.002572 0.003018 0.003869 0.002512 0.001876
Endosome 0.000282 0.000328 0.000064 0.000609 0.000188 0.001077 0.000055 0.005143 0.000237 0.000777 0.000059 0.001961
Golgi 0.000125 0.000333 0.000109 0.000651 0.000146 0.000496 0.000082 0.002423 0.000135 0.000430 0.000096 0.001180
Lipid Particles 0.000263 0.001406 0.000076 0.000479 0.000143 0.000152 0.000050 0.000425 0.000205 0.000655 0.000063 0.000463
Mitochondria 0.001921 0.001426 0.001731 0.001711 0.000461 0.001943 0.001490 0.003998 0.001214 0.001736 0.001614 0.002393
Mitotic Spindle 0.000880 0.001072 0.000143 0.009161 0.001088 0.002608 0.000111 0.013077 0.000981 0.001993 0.000128 0.010329
None 0.005620 0.018153 0.000712 0.044168 0.002136 0.001470 0.000666 0.004548 0.003933 0.008153 0.000690 0.032357
Nuclear Periphery 0.000079 0.000156 0.000110 0.001071 0.000087 0.000222 0.000109 0.000583 0.000083 0.000196 0.000109 0.000926
Nuclear Periphery Foci 0.000082 0.000695 0.000036 0.000755 0.000153 0.000404 0.000019 0.000761 0.000116 0.000520 0.000027 0.000757
Nucleolus 0.043254 0.030245 0.030813 0.008727 0.027534 0.027097 0.021042 0.008263 0.035642 0.028358 0.026086 0.008589
Nucleus 0.937562* 0.921412* 0.944693* 0.845903* 0.956166* 0.944867* 0.957639* 0.919701* 0.946571* 0.935472* 0.950956* 0.867904*
Peroxisomes 0.000526 0.000739 0.002486 0.001023 0.000818 0.000644 0.001338 0.000785 0.000667 0.000682 0.001930 0.000952
Vacuole 0.000451 0.001045 0.001107 0.003512 0.000215 0.000900 0.000934 0.004814 0.000337 0.000958 0.001023 0.003900
Vacuole Periphery 0.000409 0.000378 0.000150 0.001756 0.000094 0.001039 0.000113 0.002819 0.000256 0.000775 0.000132 0.002073

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.29 -11.86 -10.75 0.17 6.23 0.42 -4.48 -3.57 -9.09 1.97 -2.61 -9.89 -5.81 -5.47 6.72
Bud Neck -5.42 -6.19 -15.58 -11.50 -5.37 -6.19 -6.10 -10.49 -7.10 -4.08 -8.46 -8.66 -18.52 -13.06 -7.00
Bud Site -2.82 -2.54 -5.35 -0.47 -4.38 -2.51 -3.70 -6.11 -2.97 -4.53 -3.73 -4.26 -7.54 -2.32 -5.82
Cell Periphery -3.51 2.71 -6.60 -5.46 -7.08 -1.73 1.05 -3.44 0.85 -4.52 -3.07 2.90 -6.80 -5.56 -7.18
Cytoplasm -3.27 2.40 -10.95 -7.86 -11.77 0.78 1.85 -2.27 -3.10 -3.80 -2.55 3.03 -11.18 -9.84 -12.02
Cytoplasmic Foci -1.66 1.45 -6.17 -5.39 -6.39 0.55 1.63 -0.81 -1.64 -2.81 -0.48 1.97 -5.90 -5.85 -6.79
Eisosomes -3.58 -10.28 -3.31 -3.29 -3.13 -2.79 -9.02 -2.79 0.52 7.13 -4.36 -13.62 -3.32 -3.27 -3.09
Endoplasmic Reticulum -4.48 -0.08 3.43 7.68 2.47 -1.26 1.74 1.49 2.41 -0.21 -4.13 1.09 4.85 8.41 2.50
Endosome -0.37 1.63 -1.47 -1.62 -4.35 -2.90 2.20 -2.78 -1.81 -2.92 -2.78 2.38 -3.21 -1.31 -3.86
Golgi -1.48 0.07 -1.84 -0.64 -1.90 -1.84 1.03 -2.02 -1.46 -2.16 -2.41 0.89 -2.67 -1.32 -2.88
Lipid Particles -1.29 1.16 -0.69 1.17 -4.25 0.18 1.47 -0.82 -0.97 -1.69 -1.21 1.61 -1.28 0.85 -4.48
Mitochondria 0.56 0.12 -0.14 -1.83 -1.18 -2.93 -11.41 -5.18 -1.86 -3.01 -1.00 -1.24 -2.61 -1.80 -3.27
Mitotic Spindle -0.26 1.02 -4.64 -4.55 -5.23 -1.34 1.45 -3.51 -3.09 -3.89 -1.36 1.75 -5.80 -5.21 -6.52
None -5.00 5.01 -7.62 -3.38 -9.53 1.21 2.57 -2.39 -3.75 -4.83 -3.75 5.62 -8.21 -6.18 -9.90
Nuclear Periphery -1.59 -1.94 -3.93 -3.59 -3.75 -3.20 -2.65 -7.25 -5.20 -6.48 -3.52 -3.21 -4.87 -4.21 -4.57
Nuclear Periphery Foci -3.52 1.16 -2.88 0.15 -3.08 -1.91 2.26 -1.62 -0.39 -2.52 -4.03 2.54 -3.14 -0.51 -3.79
Nucleolus 5.17 2.79 15.32 11.05 4.67 0.28 1.27 10.36 11.37 2.68 4.66 2.90 18.00 15.38 5.19
Nucleus 3.18 -0.99 10.32 7.22 9.92 2.80 0.61 4.74 3.17 3.66 3.50 -0.31 12.19 9.72 11.00
Peroxisomes -0.76 -5.80 -0.54 -0.11 3.18 0.75 -0.92 0.90 0.18 3.28 0.19 -4.50 -0.03 -0.17 3.23
Vacuole -2.78 -3.96 -4.52 -3.62 -3.31 -4.08 -3.08 -4.31 -3.38 -1.93 -4.51 -4.40 -6.03 -4.82 -3.50
Vacuole Periphery 0.20 1.84 -1.31 -1.39 -1.75 -2.64 -2.48 -2.46 -0.82 -2.41 -2.29 1.61 -2.32 -1.11 -2.59
ORF
Human Ortholog
Description Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

YBR138C

YBR138C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr138c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available