Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar serine-type carboxypeptidase; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner; N-glycosylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 32 | 16 | 13 | 17 | 33 | 39 | 91 | 57 | 71 | 58 | 90 | 81 | 200 | 274 | 30 | 25 | 12 | 0 | 2 | 1 | 0 | 3 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 0 | 0 | 1 | 0 | 2 | 1 | 7 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Endosome | 1 | 4 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 25 | 5 | 8 | 4 | 1 | 10 | 13 | 3 | 3 | 10 | 11 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 1 | 1 | 2 | 1 | 0 | 0 | 2 |
Mitochondria | 76 | 29 | 31 | 2 | 15 | 18 | 21 | 24 | 14 | 29 | 21 | 30 | 1 | 2 | 2 | 24 | 3 | 1 | 1 | 5 | 1 | 1 | 2 | 3 |
Nucleus | 65 | 79 | 70 | 40 | 41 | 105 | 134 | 194 | 100 | 116 | 62 | 86 | 16 | 6 | 7 | 149 | 75 | 46 | 4 | 4 | 2 | 2 | 3 | 5 |
Nuclear Periphery | 3 | 4 | 1 | 1 | 3 | 2 | 3 | 1 | 2 | 5 | 0 | 0 | 0 | 1 | 0 | 6 | 3 | 2 | 4 | 3 | 0 | 1 | 4 | 2 |
Nucleolus | 4 | 2 | 0 | 0 | 3 | 2 | 4 | 2 | 0 | 1 | 2 | 2 | 1 | 1 | 0 | 8 | 6 | 1 | 1 | 1 | 0 | 1 | 1 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | 1 |
Vac/Vac Membrane | 127 | 298 | 117 | 63 | 155 | 175 | 192 | 251 | 188 | 236 | 206 | 255 | 107 | 156 | 103 | 161 | 91 | 58 | 199 | 362 | 175 | 120 | 212 | 128 |
Unique Cell Count | 216 | 384 | 194 | 97 | 207 | 287 | 351 | 485 | 283 | 365 | 285 | 365 | 177 | 331 | 341 | 330 | 183 | 109 | 227 | 404 | 191 | 140 | 246 | 170 |
Labelled Cell Count | 282 | 450 | 235 | 122 | 236 | 337 | 394 | 569 | 365 | 460 | 349 | 464 | 216 | 395 | 392 | 395 | 221 | 122 | 227 | 404 | 191 | 140 | 246 | 170 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.6 | 6.3 | 7.2 | 10.0 | 10.8 | 9.1 | 9.8 | 9.7 | 8.8 | 8.8 | 8.5 | 5.1 | 5.0 | 4.6 | 10.0 | 13.6 | 14.0 | 5.5 | 5.6 | 6.5 |
Std Deviation (1e-4) | 1.1 | 1.5 | 2.5 | 2.5 | 2.3 | 4.1 | 3.4 | 3.8 | 3.6 | 3.3 | 3.0 | 2.7 | 1.4 | 1.5 | 1.1 | 3.1 | 4.4 | 5.3 | 1.3 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.18 | 0.66 | 0.77 | 0.52 | 0.63 | 0.62 | 0.48 | 0.48 | 0.42 | -0.31 | -0.35 | -0.45 | 0.65 | 1.1 | 1.14 | -0.2 | -0.19 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 0 | 1.2 | 1.1 | 3.4 | 3.5 | 3.5 | 3.6 | 4.7 | 8.2 | 11.7 | 16.1 | 0.3 | 1.7 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.5 | -2.7 | -3.5 | -3.8 | -4.8 | -4.0 | -2.9 | -3.0 | -2.8 | -5.3 | -7.0 | 0 | -3.1 | -4.9 | -4.1 |
Nucleus | 0.9 | -3.6 | 0.1 | 0.5 | 0.9 | -0.2 | -1.0 | -3.4 | -3.1 | -6.2 | -10.8 | -10.8 | 2.0 | 1.0 | 1.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.8 | 3.1 | 0.1 | -1.3 | -2.0 | 1.4 | 1.0 | 2.7 | 2.3 | 0 | -2.9 | -6.8 | -2.6 | -2.1 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4324 | 2.9202 | 2.1776 | 1.3712 | 1.6339 | 2.5689 | 2.8086 | 3.6392 | 3.1214 | 3.4996 | 3.1168 | 3.6159 | 3.518 | 3.8733 | 3.2105 | 3.5121 | 2.6628 | 3.818 |
Actin | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0137 | 0.0001 | 0.0111 | 0.0008 | 0.0018 | 0 | 0.0054 | 0.0001 | 0.0182 | 0.0975 | 0.0057 | 0.0003 |
Bud | 0.0004 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0004 | 0.0006 | 0.0025 | 0.0004 | 0.0003 | 0.0008 | 0.0001 | 0.0004 | 0.0003 | 0.0016 | 0.0005 |
Bud Neck | 0.0016 | 0.0003 | 0.0012 | 0.0005 | 0.0018 | 0.0011 | 0.0012 | 0.0007 | 0.0015 | 0.0014 | 0.001 | 0.0016 | 0.0073 | 0.0003 | 0.0008 | 0.0009 | 0.0022 | 0.0018 |
Bud Periphery | 0.001 | 0.0008 | 0.0012 | 0.0013 | 0.0008 | 0.0013 | 0.0015 | 0.0012 | 0.0016 | 0.0043 | 0.0013 | 0.0011 | 0.0019 | 0.0005 | 0.0014 | 0.0012 | 0.0028 | 0.0017 |
Bud Site | 0.0018 | 0.0004 | 0.0029 | 0.0007 | 0.0027 | 0.0006 | 0.0093 | 0.0012 | 0.0034 | 0.024 | 0.0008 | 0.0008 | 0.0067 | 0.0004 | 0.0017 | 0.0009 | 0.003 | 0.001 |
Cell Periphery | 0.004 | 0.0014 | 0.0039 | 0.0016 | 0.003 | 0.0031 | 0.0026 | 0.0032 | 0.0025 | 0.0022 | 0.0026 | 0.003 | 0.0046 | 0.002 | 0.0022 | 0.0015 | 0.0046 | 0.0025 |
Cytoplasm | 0.0034 | 0.0298 | 0.0044 | 0.0138 | 0.008 | 0.0028 | 0.0077 | 0.0075 | 0.0087 | 0.0056 | 0.0081 | 0.0048 | 0.0122 | 0.0248 | 0.017 | 0.102 | 0.0169 | 0.0023 |
Cytoplasmic Foci | 0.012 | 0.0128 | 0.0088 | 0.0299 | 0.0105 | 0.0033 | 0.0194 | 0.0105 | 0.018 | 0.0415 | 0.0203 | 0.0098 | 0.05 | 0.0062 | 0.0388 | 0.0082 | 0.0226 | 0.0072 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0005 | 0.0004 | 0 | 0 |
Endoplasmic Reticulum | 0.0026 | 0.0022 | 0.0026 | 0.0036 | 0.0017 | 0.0013 | 0.0047 | 0.0075 | 0.0034 | 0.0026 | 0.0062 | 0.0019 | 0.0141 | 0.0048 | 0.0021 | 0.0013 | 0.0016 | 0.0031 |
Endosome | 0.1613 | 0.1603 | 0.1768 | 0.2698 | 0.2093 | 0.1104 | 0.2231 | 0.1787 | 0.2268 | 0.1952 | 0.2517 | 0.2159 | 0.2598 | 0.0857 | 0.1772 | 0.1544 | 0.2907 | 0.1752 |
Golgi | 0.0111 | 0.0035 | 0.0084 | 0.0215 | 0.0046 | 0.0052 | 0.0193 | 0.0059 | 0.011 | 0.0096 | 0.0272 | 0.0077 | 0.0495 | 0.0024 | 0.0073 | 0.007 | 0.046 | 0.0075 |
Lipid Particles | 0.0647 | 0.0092 | 0.0119 | 0.0286 | 0.0238 | 0.039 | 0.0325 | 0.016 | 0.0176 | 0.0482 | 0.0158 | 0.019 | 0.112 | 0.0403 | 0.0256 | 0.0144 | 0.0061 | 0.0483 |
Mitochondria | 0.0178 | 0.0014 | 0.0351 | 0.0174 | 0.0009 | 0.0285 | 0.036 | 0.0424 | 0.0808 | 0.0091 | 0.0561 | 0.0105 | 0.0478 | 0.0137 | 0.0269 | 0.0701 | 0.0295 | 0.0799 |
None | 0.0437 | 0.0099 | 0.0173 | 0.014 | 0.0053 | 0.0136 | 0.0034 | 0.0229 | 0.028 | 0.0039 | 0.0228 | 0.0006 | 0.0106 | 0.0048 | 0.0888 | 0.1013 | 0.0003 | 0.0029 |
Nuclear Periphery | 0.0099 | 0.0038 | 0.0096 | 0.0043 | 0.0024 | 0.0039 | 0.0142 | 0.0155 | 0.0035 | 0.0038 | 0.0023 | 0.0041 | 0.0096 | 0.0212 | 0.003 | 0.0012 | 0.0021 | 0.0099 |
Nucleolus | 0.016 | 0.0009 | 0.0082 | 0.003 | 0.0131 | 0.0038 | 0.0038 | 0.0041 | 0.005 | 0.014 | 0.0041 | 0.0039 | 0.0236 | 0.0019 | 0.004 | 0.0031 | 0.0048 | 0.0037 |
Nucleus | 0.0059 | 0.0032 | 0.0274 | 0.0033 | 0.0254 | 0.0041 | 0.0062 | 0.006 | 0.0088 | 0.0052 | 0.0027 | 0.0046 | 0.0093 | 0.0104 | 0.0028 | 0.0021 | 0.0062 | 0.0043 |
Peroxisomes | 0.0109 | 0.0008 | 0.0015 | 0.0053 | 0.0022 | 0.0007 | 0.0102 | 0.0022 | 0.0073 | 0.0155 | 0.0023 | 0.0013 | 0.0086 | 0.001 | 0.0121 | 0.0013 | 0.002 | 0.0028 |
Punctate Nuclear | 0.0005 | 0.0003 | 0.0002 | 0.0012 | 0.0007 | 0.0002 | 0.001 | 0.0004 | 0.0014 | 0.0025 | 0.0004 | 0.0002 | 0.0022 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.0002 |
Vacuole | 0.476 | 0.5585 | 0.5083 | 0.4787 | 0.5947 | 0.5926 | 0.4513 | 0.5213 | 0.3944 | 0.4872 | 0.4843 | 0.5583 | 0.3028 | 0.5412 | 0.4469 | 0.3728 | 0.4667 | 0.4877 |
Vacuole Periphery | 0.1549 | 0.2004 | 0.1697 | 0.1006 | 0.0886 | 0.1842 | 0.1386 | 0.1521 | 0.1637 | 0.1207 | 0.0877 | 0.1506 | 0.0606 | 0.2379 | 0.1209 | 0.0578 | 0.0843 | 0.1571 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.5101 | 17.4408 | 49.8501 | 71.0213 | 17.8007 | 23.1103 | 40.004 | 75.3796 | 83.5272 | 40.2992 |
Translational Efficiency | 1.3001 | 1.2141 | 1.2729 | 1.0325 | 1.4844 | 1.5306 | 1.3175 | 0.9399 | 1.1625 | 1.0928 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1906 | 157 | 1115 | 1059 | 2149 | 514 | 2291 | 153 | 4055 | 671 | 3406 | 1212 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 799.65 | 862.84 | 797.92 | 891.10 | 787.79 | 758.22 | 897.40 | 966.77 | 793.36 | 782.70 | 864.83 | 900.65 |
Standard Deviation | 108.59 | 261.05 | 104.60 | 106.27 | 102.25 | 93.26 | 100.38 | 142.13 | 105.45 | 156.74 | 111.97 | 114.23 |
Intensity Change Log 2 | — | 0.109724 | -0.003125 | 0.156219 | — | -0.055195 | 0.187940 | 0.295362 | — | 0.030235 | 0.094856 | 0.226947 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000013 | 0.001418 | 0.000189 | 0.000333 | 0.000012 | 0.000077 | 0.000053 | 0.000191 | 0.000013 | 0.000391 | 0.000098 | 0.000315 |
Bud Neck | 0.011116 | 0.019229 | 0.036985 | 0.020581 | 0.010456 | 0.025440 | 0.034731 | 0.032951 | 0.010767 | 0.023987 | 0.035469 | 0.022143 |
Bud Site | 0.006673 | 0.014518 | 0.009190 | 0.023607 | 0.006124 | 0.013518 | 0.016680 | 0.042366 | 0.006382 | 0.013752 | 0.014228 | 0.025975 |
Cell Periphery | 0.001665 | 0.000419 | 0.000104 | 0.000072 | 0.000524 | 0.000401 | 0.000079 | 0.000251 | 0.001060 | 0.000405 | 0.000087 | 0.000095 |
Cytoplasm | 0.005852 | 0.074847 | 0.136383* | 0.232356* | 0.013384 | 0.060789 | 0.116325* | 0.076323 | 0.009843 | 0.064078 | 0.122891* | 0.212659* |
Cytoplasmic Foci | 0.003415 | 0.042736 | 0.020276 | 0.073009 | 0.007048 | 0.040996 | 0.023837 | 0.021203 | 0.005340 | 0.041403 | 0.022671 | 0.066469 |
Eisosomes | 0.000022 | 0.000061 | 0.000052 | 0.000038 | 0.000011 | 0.000050 | 0.000022 | 0.000035 | 0.000017 | 0.000053 | 0.000032 | 0.000037 |
Endoplasmic Reticulum | 0.002463 | 0.002984 | 0.006993 | 0.001061 | 0.002616 | 0.001056 | 0.002638 | 0.000875 | 0.002544 | 0.001507 | 0.004063 | 0.001038 |
Endosome | 0.070084 | 0.068154 | 0.077222 | 0.075012 | 0.058457 | 0.095497 | 0.092952 | 0.076073 | 0.063922 | 0.089099 | 0.087802 | 0.075146 |
Golgi | 0.001202 | 0.002020 | 0.000768 | 0.000404 | 0.001055 | 0.002841 | 0.000735 | 0.000959 | 0.001124 | 0.002649 | 0.000746 | 0.000474 |
Lipid Particles | 0.000422 | 0.002418 | 0.003798 | 0.002039 | 0.000857 | 0.002107 | 0.001889 | 0.001198 | 0.000652 | 0.002180 | 0.002514 | 0.001933 |
Mitochondria | 0.001810 | 0.002071 | 0.002230 | 0.000176 | 0.001293 | 0.003742 | 0.000977 | 0.000328 | 0.001536 | 0.003351 | 0.001387 | 0.000195 |
Mitotic Spindle | 0.000063 | 0.000232 | 0.010029 | 0.007716 | 0.000821 | 0.000764 | 0.005088 | 0.024965 | 0.000464 | 0.000640 | 0.006705 | 0.009894 |
None | 0.000090 | 0.000328 | 0.002269 | 0.001195 | 0.000103 | 0.000512 | 0.000478 | 0.000582 | 0.000097 | 0.000469 | 0.001064 | 0.001118 |
Nuclear Periphery | 0.000080 | 0.000422 | 0.007242 | 0.000884 | 0.000255 | 0.000151 | 0.002116 | 0.000572 | 0.000173 | 0.000215 | 0.003794 | 0.000845 |
Nuclear Periphery Foci | 0.000086 | 0.000597 | 0.003277 | 0.002576 | 0.000121 | 0.000160 | 0.001792 | 0.000804 | 0.000104 | 0.000262 | 0.002278 | 0.002352 |
Nucleolus | 0.000748 | 0.002624 | 0.021561 | 0.001481 | 0.000586 | 0.000803 | 0.005731 | 0.002387 | 0.000662 | 0.001229 | 0.010913 | 0.001595 |
Nucleus | 0.002433 | 0.028571 | 0.315670* | 0.031860 | 0.003690 | 0.012142 | 0.078383 | 0.045201 | 0.003099 | 0.015986 | 0.156062* | 0.033544 |
Peroxisomes | 0.000800 | 0.005172 | 0.003480 | 0.010876 | 0.000640 | 0.005257 | 0.002973 | 0.003439 | 0.000715 | 0.005237 | 0.003139 | 0.009937 |
Vacuole | 0.851727* | 0.712159* | 0.335052* | 0.513096* | 0.859393* | 0.715978* | 0.604283* | 0.658111* | 0.855790* | 0.715084* | 0.516147* | 0.531402* |
Vacuole Periphery | 0.039237 | 0.019019 | 0.007230 | 0.001628 | 0.032554 | 0.017719 | 0.008238 | 0.011185 | 0.035695 | 0.018023 | 0.007908 | 0.002834 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.16 | -9.32 | -4.23 | 0.97 | -0.46 | -3.44 | -10.15 | -2.04 | -1.30 | -1.44 | -1.34 | -12.55 | -4.63 | 0.52 | -2.46 |
Bud Neck | -1.63 | -10.15 | -1.39 | 1.10 | 8.59 | -4.70 | -13.37 | -4.12 | -0.75 | 1.37 | -4.93 | -16.87 | -3.05 | 2.82 | 10.75 |
Bud Site | -2.63 | -2.09 | -5.02 | 0.11 | -3.26 | -4.02 | -6.68 | -3.49 | -2.53 | -2.32 | -4.77 | -7.03 | -6.32 | -1.38 | -1.87 |
Cell Periphery | 8.40 | 11.49 | 11.85 | 6.14 | 4.94 | 1.86 | 11.57 | 2.71 | 1.57 | -1.07 | 8.07 | 14.44 | 14.26 | 6.92 | 0.59 |
Cytoplasm | -4.85 | -24.18 | -29.37 | -9.22 | -8.83 | -7.62 | -29.10 | -6.69 | -2.83 | 2.31 | -9.38 | -38.21 | -29.24 | -16.12 | -9.42 |
Cytoplasmic Foci | -5.30 | -13.18 | -15.77 | -0.01 | -8.53 | -7.77 | -12.65 | -4.25 | 2.70 | -0.36 | -9.59 | -18.06 | -15.62 | 0.33 | -7.68 |
Eisosomes | -3.11 | -5.11 | -2.55 | 2.23 | 6.41 | -4.48 | -8.56 | -5.68 | 0.71 | -3.76 | -4.84 | -5.61 | -7.09 | 2.23 | -3.28 |
Endoplasmic Reticulum | -0.65 | -14.68 | 9.26 | 2.34 | 20.41 | 5.01 | 0.09 | 3.21 | -1.28 | 4.32 | 3.93 | -7.17 | 7.85 | 1.53 | 20.13 |
Endosome | 0.21 | 0.73 | 8.90 | 3.43 | 7.08 | -5.08 | -5.54 | -0.27 | 2.99 | 2.02 | -4.07 | -3.71 | 8.45 | 8.46 | 11.01 |
Golgi | -0.78 | 2.14 | 4.60 | 1.80 | 3.42 | -1.21 | 1.50 | 0.71 | 1.43 | -0.29 | -1.33 | 2.55 | 4.50 | 2.12 | 4.25 |
Lipid Particles | -3.32 | -14.40 | -6.95 | 1.34 | 8.23 | -2.83 | -4.70 | -1.83 | 0.68 | 0.67 | -4.43 | -11.99 | -5.42 | 1.50 | 5.37 |
Mitochondria | -0.20 | -0.78 | 3.84 | 1.67 | 11.79 | -1.97 | 0.72 | 2.43 | 2.85 | 3.49 | -1.83 | 0.50 | 4.76 | 3.33 | 10.20 |
Mitotic Spindle | -1.32 | -5.36 | -4.62 | -4.52 | 0.75 | 0.06 | -5.67 | -2.76 | -2.77 | -2.13 | -0.37 | -8.06 | -5.15 | -4.98 | -1.29 |
None | -1.90 | -8.74 | -5.94 | -3.53 | 4.26 | -1.39 | -7.25 | -2.87 | -0.55 | -0.82 | -1.64 | -10.91 | -6.39 | -1.98 | 0.68 |
Nuclear Periphery | -2.95 | -14.08 | -13.17 | -6.38 | 11.44 | 1.47 | -9.22 | -3.02 | -3.40 | 3.73 | -0.95 | -16.44 | -11.87 | -11.54 | 11.02 |
Nuclear Periphery Foci | -2.40 | -5.88 | -10.88 | -5.91 | 2.63 | -1.55 | -4.66 | -3.36 | -3.21 | -0.15 | -3.03 | -7.46 | -11.29 | -9.92 | 0.81 |
Nucleolus | -1.11 | -10.57 | -0.76 | 1.00 | 10.46 | -1.41 | -10.60 | -1.76 | -1.48 | 3.04 | -1.44 | -14.06 | -2.01 | 0.36 | 13.02 |
Nucleus | -3.12 | -35.81 | -17.93 | -3.21 | 27.69 | -3.38 | -26.65 | -4.64 | -3.66 | 3.48 | -4.70 | -39.55 | -18.11 | -10.11 | 20.71 |
Peroxisomes | -2.57 | -5.56 | -4.90 | 0.22 | -1.93 | -3.11 | -5.26 | -2.32 | 1.12 | -0.44 | -3.76 | -7.18 | -5.45 | 0.54 | -2.29 |
Vacuole | 5.57 | 54.43* | 26.39 | 4.63 | -20.36 | 10.42 | 37.33* | 8.49 | 2.44 | -2.29 | 11.76 | 57.60* | 29.08 | 8.27 | -8.84 |
Vacuole Periphery | 4.62 | 19.99 | 24.26* | 4.32 | 10.09 | 7.58 | 19.43 | 7.96 | 2.94 | -0.42 | 9.81 | 27.67 | 31.92 | 10.22 | 9.68 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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