Standard name
Human Ortholog
Description Vacuolar serine-type carboxypeptidase; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner; N-glycosylated

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.08 0.13 0.08 0.11 0.11 0.19 0.2 0.19 0.2 0.25 0.46 0.6 0.8 0.09 0.14 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0.05 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.35 0.08 0.16 0 0.07 0.06 0.06 0.05 0.05 0.08 0.07 0.08 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.3 0.21 0.36 0.41 0.2 0.37 0.38 0.4 0.35 0.32 0.22 0.24 0.09 0 0 0.45 0.41 0.42 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.78 0.6 0.65 0.75 0.61 0.55 0.52 0.66 0.65 0.72 0.7 0.6 0.47 0.3 0.49 0.5 0.53 0.88 0.9 0.92 0.86 0.86 0.76
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 0 0 0 1 2 1 0 0 1 2 0 0 0 0 1 2 2 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 32 16 13 17 33 39 91 57 71 58 90 81 200 274 30 25 12 0 2 1 0 3 1
Endoplasmic Reticulum 0 1 0 1 0 0 1 4 2 0 0 1 0 2 1 7 6 0 0 1 0 0 0 1
Endosome 1 4 0 2 2 2 0 0 0 0 0 0 9 25 5 8 4 1 10 13 3 3 10 11
Golgi 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 2 6 1 1 2 1 0 0 2
Mitochondria 76 29 31 2 15 18 21 24 14 29 21 30 1 2 2 24 3 1 1 5 1 1 2 3
Nucleus 65 79 70 40 41 105 134 194 100 116 62 86 16 6 7 149 75 46 4 4 2 2 3 5
Nuclear Periphery 3 4 1 1 3 2 3 1 2 5 0 0 0 1 0 6 3 2 4 3 0 1 4 2
Nucleolus 4 2 0 0 3 2 4 2 0 1 2 2 1 1 0 8 6 1 1 1 0 1 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 1 1 0 1
Vac/Vac Membrane 127 298 117 63 155 175 192 251 188 236 206 255 107 156 103 161 91 58 199 362 175 120 212 128
Unique Cell Count 216 384 194 97 207 287 351 485 283 365 285 365 177 331 341 330 183 109 227 404 191 140 246 170
Labelled Cell Count 282 450 235 122 236 337 394 569 365 460 349 464 216 395 392 395 221 122 227 404 191 140 246 170


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.6 6.3 7.2 10.0 10.8 9.1 9.8 9.7 8.8 8.8 8.5 5.1 5.0 4.6 10.0 13.6 14.0 5.5 5.6 6.5
Std Deviation (1e-4) 1.1 1.5 2.5 2.5 2.3 4.1 3.4 3.8 3.6 3.3 3.0 2.7 1.4 1.5 1.1 3.1 4.4 5.3 1.3 1.9 2.0
Intensity Change (Log2) 0.18 0.66 0.77 0.52 0.63 0.62 0.48 0.48 0.42 -0.31 -0.35 -0.45 0.65 1.1 1.14 -0.2 -0.19 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0 1.2 1.1 3.4 3.5 3.5 3.6 4.7 8.2 11.7 16.1 0.3 1.7 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 3.9 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.5 -2.7 -3.5 -3.8 -4.8 -4.0 -2.9 -3.0 -2.8 -5.3 -7.0 0 -3.1 -4.9 -4.1
Nucleus 0.9 -3.6 0.1 0.5 0.9 -0.2 -1.0 -3.4 -3.1 -6.2 -10.8 -10.8 2.0 1.0 1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 3.1 0.1 -1.3 -2.0 1.4 1.0 2.7 2.3 0 -2.9 -6.8 -2.6 -2.1 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4324 2.9202 2.1776 1.3712 1.6339 2.5689 2.8086 3.6392 3.1214 3.4996 3.1168 3.6159 3.518 3.8733 3.2105 3.5121 2.6628 3.818
Actin 0.0001 0 0.0001 0.0002 0 0 0.0137 0.0001 0.0111 0.0008 0.0018 0 0.0054 0.0001 0.0182 0.0975 0.0057 0.0003
Bud 0.0004 0.0003 0.0004 0.0005 0.0003 0.0003 0.0006 0.0004 0.0006 0.0025 0.0004 0.0003 0.0008 0.0001 0.0004 0.0003 0.0016 0.0005
Bud Neck 0.0016 0.0003 0.0012 0.0005 0.0018 0.0011 0.0012 0.0007 0.0015 0.0014 0.001 0.0016 0.0073 0.0003 0.0008 0.0009 0.0022 0.0018
Bud Periphery 0.001 0.0008 0.0012 0.0013 0.0008 0.0013 0.0015 0.0012 0.0016 0.0043 0.0013 0.0011 0.0019 0.0005 0.0014 0.0012 0.0028 0.0017
Bud Site 0.0018 0.0004 0.0029 0.0007 0.0027 0.0006 0.0093 0.0012 0.0034 0.024 0.0008 0.0008 0.0067 0.0004 0.0017 0.0009 0.003 0.001
Cell Periphery 0.004 0.0014 0.0039 0.0016 0.003 0.0031 0.0026 0.0032 0.0025 0.0022 0.0026 0.003 0.0046 0.002 0.0022 0.0015 0.0046 0.0025
Cytoplasm 0.0034 0.0298 0.0044 0.0138 0.008 0.0028 0.0077 0.0075 0.0087 0.0056 0.0081 0.0048 0.0122 0.0248 0.017 0.102 0.0169 0.0023
Cytoplasmic Foci 0.012 0.0128 0.0088 0.0299 0.0105 0.0033 0.0194 0.0105 0.018 0.0415 0.0203 0.0098 0.05 0.0062 0.0388 0.0082 0.0226 0.0072
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0.0007 0.0001 0.0001 0 0.0003 0 0.0005 0.0004 0 0
Endoplasmic Reticulum 0.0026 0.0022 0.0026 0.0036 0.0017 0.0013 0.0047 0.0075 0.0034 0.0026 0.0062 0.0019 0.0141 0.0048 0.0021 0.0013 0.0016 0.0031
Endosome 0.1613 0.1603 0.1768 0.2698 0.2093 0.1104 0.2231 0.1787 0.2268 0.1952 0.2517 0.2159 0.2598 0.0857 0.1772 0.1544 0.2907 0.1752
Golgi 0.0111 0.0035 0.0084 0.0215 0.0046 0.0052 0.0193 0.0059 0.011 0.0096 0.0272 0.0077 0.0495 0.0024 0.0073 0.007 0.046 0.0075
Lipid Particles 0.0647 0.0092 0.0119 0.0286 0.0238 0.039 0.0325 0.016 0.0176 0.0482 0.0158 0.019 0.112 0.0403 0.0256 0.0144 0.0061 0.0483
Mitochondria 0.0178 0.0014 0.0351 0.0174 0.0009 0.0285 0.036 0.0424 0.0808 0.0091 0.0561 0.0105 0.0478 0.0137 0.0269 0.0701 0.0295 0.0799
None 0.0437 0.0099 0.0173 0.014 0.0053 0.0136 0.0034 0.0229 0.028 0.0039 0.0228 0.0006 0.0106 0.0048 0.0888 0.1013 0.0003 0.0029
Nuclear Periphery 0.0099 0.0038 0.0096 0.0043 0.0024 0.0039 0.0142 0.0155 0.0035 0.0038 0.0023 0.0041 0.0096 0.0212 0.003 0.0012 0.0021 0.0099
Nucleolus 0.016 0.0009 0.0082 0.003 0.0131 0.0038 0.0038 0.0041 0.005 0.014 0.0041 0.0039 0.0236 0.0019 0.004 0.0031 0.0048 0.0037
Nucleus 0.0059 0.0032 0.0274 0.0033 0.0254 0.0041 0.0062 0.006 0.0088 0.0052 0.0027 0.0046 0.0093 0.0104 0.0028 0.0021 0.0062 0.0043
Peroxisomes 0.0109 0.0008 0.0015 0.0053 0.0022 0.0007 0.0102 0.0022 0.0073 0.0155 0.0023 0.0013 0.0086 0.001 0.0121 0.0013 0.002 0.0028
Punctate Nuclear 0.0005 0.0003 0.0002 0.0012 0.0007 0.0002 0.001 0.0004 0.0014 0.0025 0.0004 0.0002 0.0022 0.0003 0.0011 0.0001 0.0002 0.0002
Vacuole 0.476 0.5585 0.5083 0.4787 0.5947 0.5926 0.4513 0.5213 0.3944 0.4872 0.4843 0.5583 0.3028 0.5412 0.4469 0.3728 0.4667 0.4877
Vacuole Periphery 0.1549 0.2004 0.1697 0.1006 0.0886 0.1842 0.1386 0.1521 0.1637 0.1207 0.0877 0.1506 0.0606 0.2379 0.1209 0.0578 0.0843 0.1571

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.5101 17.4408 49.8501 71.0213 17.8007 23.1103 40.004 75.3796 83.5272 40.2992
Translational Efficiency 1.3001 1.2141 1.2729 1.0325 1.4844 1.5306 1.3175 0.9399 1.1625 1.0928

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Vacuolar serine-type carboxypeptidase; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner; N-glycosylated
Localization
Cell Percentages vacuole (95%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg42

Atg42


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg42-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available