Standard name
Human Ortholog
Description Protein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0.05 0 0.05 0.05 0.07 0 0.05 0 0.1 0.1 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.99 0.98 0.92 0.9 0.85 0.9 0.81 0.9 0.99 0.99 1.0 0.95 0.97 0.96 0.94 0.94 0.96 0.98 0.96 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.05 0.14 0.08 0.16 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 1 1 0 0 2 0 1 2 6 6 8 3 0 0 0 0 1 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 0 4 13 7 16 16 7 12 11 12 3 24 18 29 11 8 0 0 0 0 0 0
Cytoplasm 165 188 94 132 275 197 350 262 157 219 173 164 381 467 490 264 104 156 158 173 110 275 435 350
Endoplasmic Reticulum 2 3 1 1 2 0 8 4 3 3 3 1 0 5 2 6 3 3 0 2 0 0 4 4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 2 1
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 0 0 2 0
Mitochondria 3 1 0 0 0 1 23 15 25 19 35 10 1 1 0 0 2 4 2 0 0 0 1 1
Nucleus 0 2 3 2 3 1 3 4 3 5 2 2 2 0 1 5 1 7 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 2 7 8 4 4 3 6 0 0 0 14 1 0 1 1 0 0 0 2
Unique Cell Count 168 192 96 134 278 201 382 292 184 244 213 183 383 471 491 279 107 162 169 184 115 280 453 368
Labelled Cell Count 172 201 98 139 295 210 409 312 205 268 236 199 387 497 511 318 124 179 169 184 115 280 453 368


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 11.0 7.8 8.1 9.2 8.3 7.8 8.3 7.1 7.3 6.9 7.8 10.5 11.8 11.9 15.7 15.2 15.3 9.4 10.1 10.8
Std Deviation (1e-4) 1.8 1.9 1.3 1.1 1.5 1.6 1.5 1.5 1.4 1.2 1.7 1.9 1.5 1.6 1.7 3.4 3.7 3.9 1.3 1.4 1.6
Intensity Change (Log2) – – – 0.05 0.23 0.09 0.0 0.09 -0.14 -0.1 -0.18 -0.01 0.43 0.59 0.61 1.0 0.96 0.97 0.27 0.37 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.3 3.2 0
Cytoplasm 0.3 0.7 0.1 -2.1 -2.5 -3.3 -2.5 -4.0 -2.5 1.5 1.1 2.4 -1.3 -0.3 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.8 2.8 4.2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2885 4.1418 3.366 3.0927 2.9896 3.6226 3.7837 4.6695 4.2231 3.709 3.367 4.0911 4.4795 5.7061 4.8788 4.5841 4.2895 5.2898
Actin 0.0396 0.0064 0.033 0.0098 0.04 0.0233 0.0444 0.0012 0.0196 0.1207 0.0849 0.0074 0.0472 0.0037 0.0139 0.0435 0.0187 0.0035
Bud 0.0011 0.0014 0.0019 0.0014 0.0006 0.0019 0.0005 0.002 0.0008 0.0004 0.0007 0.0036 0.0004 0.0003 0.0074 0.0002 0.0019 0.0023
Bud Neck 0.0074 0.0007 0.0077 0.0012 0.0019 0.0018 0.0073 0.0002 0.0011 0.0023 0.0026 0.0021 0.0018 0.0004 0.0007 0.0007 0.0095 0.0014
Bud Periphery 0.0012 0.0004 0.0019 0.0034 0.0014 0.0027 0.0006 0.001 0.0017 0.0003 0.0009 0.0034 0.0004 0.0004 0.0011 0.0001 0.0038 0.0014
Bud Site 0.0156 0.014 0.0245 0.0017 0.0018 0.0006 0.003 0.007 0.0021 0.0017 0.0128 0.0008 0.0038 0.0028 0.0029 0.0005 0.0036 0.0001
Cell Periphery 0.0005 0.0003 0.0014 0.0007 0.0002 0.0002 0.0004 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0005 0.0001 0.0001 0.0006 0.0007
Cytoplasm 0.6839 0.9147 0.7777 0.8761 0.6709 0.8406 0.8064 0.9417 0.882 0.7637 0.7458 0.9182 0.8542 0.9724 0.9457 0.938 0.8236 0.9641
Cytoplasmic Foci 0.0347 0.005 0.0243 0.0101 0.034 0.022 0.0212 0.0019 0.0086 0.0206 0.0197 0.0093 0.0155 0.0027 0.0032 0.0035 0.0364 0.003
Eisosomes 0.0005 0 0.0003 0.0001 0.0004 0.0001 0.0009 0 0.0001 0.0008 0.0002 0 0.0004 0 0.0001 0.0001 0.0007 0
Endoplasmic Reticulum 0.007 0.0022 0.0073 0.0082 0.0041 0.0074 0.0038 0.0021 0.0039 0.0031 0.0026 0.0015 0.0046 0.0056 0.003 0.0018 0.0066 0.0025
Endosome 0.0418 0.001 0.0226 0.0086 0.0232 0.0123 0.0157 0.0007 0.014 0.0068 0.0282 0.0035 0.0119 0.0004 0.0023 0.0005 0.0062 0.0068
Golgi 0.0083 0.0004 0.0081 0.005 0.0197 0.0053 0.0054 0.0001 0.007 0.0144 0.0099 0.0023 0.0066 0.0002 0.0009 0.0007 0.0041 0.0045
Lipid Particles 0.0193 0.0001 0.0076 0.0097 0.039 0.0094 0.0093 0.0001 0.0033 0.0181 0.0428 0.0032 0.0066 0.0001 0.0004 0.0001 0.0157 0.0013
Mitochondria 0.0182 0.0002 0.0041 0.0085 0.005 0.0014 0.0024 0.0001 0.0028 0.0015 0.0037 0.0058 0.0025 0.0001 0.0086 0.0002 0.0241 0.0011
None 0.0599 0.0471 0.0515 0.0302 0.0963 0.053 0.0554 0.0392 0.0396 0.0293 0.0037 0.0212 0.0195 0.0062 0.0039 0.005 0.0028 0.0028
Nuclear Periphery 0.0149 0.0007 0.0031 0.0024 0.0071 0.0043 0.0045 0.0004 0.0012 0.0008 0.0017 0.0005 0.0089 0.0012 0.0009 0.0005 0.0035 0.0009
Nucleolus 0.0014 0.0002 0.002 0.0001 0.0004 0.0004 0.001 0 0.002 0.0001 0.0014 0.0001 0.0005 0 0.0002 0 0.0004 0
Nucleus 0.006 0.0032 0.0037 0.0035 0.0019 0.0037 0.003 0.0014 0.0032 0.001 0.002 0.0019 0.0023 0.0019 0.0021 0.002 0.0062 0.0016
Peroxisomes 0.02 0.0002 0.0085 0.0143 0.0418 0.0028 0.0077 0 0.0035 0.012 0.026 0.0127 0.0055 0.0001 0.0008 0.0019 0.0187 0.0007
Punctate Nuclear 0.0137 0.001 0.0054 0.0018 0.0079 0.0048 0.0052 0.0003 0.0009 0.0018 0.0084 0.002 0.0056 0.0005 0.0016 0.0003 0.0074 0.0005
Vacuole 0.0033 0.0008 0.0027 0.0029 0.0012 0.0015 0.0012 0.0004 0.0019 0.0003 0.0015 0.0004 0.0007 0.0004 0.0004 0.0003 0.0047 0.0006
Vacuole Periphery 0.0016 0.0001 0.0007 0.0006 0.0012 0.0005 0.0005 0 0.0004 0.0002 0.0005 0.0001 0.0007 0 0.0001 0 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.6475 41.488 46.9484 56.4975 50.3949 49.8634 50.3381 56.9786 66.8742 50.5817
Translational Efficiency 1.4936 1.1226 1.0341 0.8461 0.8958 1.3845 1.0373 1.0055 0.9122 0.978

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; localizes to both cytoplasm and nucleus
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Apd1

Apd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available