Standard name | |
---|---|
Human Ortholog | |
Description | TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 |
Bud | 0 | 0 | 3 | 5 | 1 | 2 | 8 | 5 | 5 | 4 | 7 | 9 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 22 | 5 | 5 | 4 | 16 | 11 | 3 | 17 | 13 | 18 | 7 | 5 | 2 | 13 | 17 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 239 | 173 | 336 | 278 | 316 | 301 | 345 | 358 | 229 | 293 | 260 | 231 | 364 | 530 | 575 | 182 | 225 | 119 | 231 | 164 | 318 | 302 | 398 | 415 |
Endoplasmic Reticulum | 1 | 0 | 12 | 2 | 2 | 4 | 7 | 7 | 1 | 13 | 1 | 3 | 0 | 4 | 2 | 6 | 10 | 5 | 2 | 1 | 18 | 0 | 3 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 2 | 5 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Mitochondria | 1 | 1 | 4 | 13 | 14 | 68 | 132 | 126 | 84 | 178 | 175 | 158 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 5 | 0 | 0 | 2 |
Nucleus | 2 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 6 | 0 | 4 | 1 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 1 | 2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 0 | 0 | 4 | 4 | 9 | 12 | 37 | 29 | 29 | 42 | 28 | 1 | 1 | 0 | 4 | 1 | 4 | 2 | 1 | 4 | 2 | 5 | 7 |
Unique Cell Count | 244 | 174 | 351 | 289 | 334 | 347 | 451 | 488 | 321 | 457 | 412 | 388 | 365 | 537 | 577 | 188 | 233 | 123 | 243 | 169 | 359 | 316 | 419 | 453 |
Labelled Cell Count | 245 | 176 | 378 | 309 | 345 | 390 | 522 | 551 | 362 | 535 | 505 | 455 | 374 | 542 | 581 | 209 | 258 | 136 | 243 | 169 | 359 | 316 | 419 | 453 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.3 | 13.9 | 11.4 | 10.4 | 9.1 | 7.9 | 7.4 | 7.2 | 6.4 | 6.6 | 6.3 | 6.3 | 12.0 | 11.8 | 10.7 | 21.6 | 22.4 | 22.0 | 12.4 | 12.6 | 12.5 |
Std Deviation (1e-4) | 1.5 | 1.9 | 1.6 | 1.8 | 1.7 | 1.5 | 1.4 | 1.4 | 1.8 | 1.6 | 1.3 | 1.6 | 1.9 | 1.6 | 1.6 | 6.0 | 5.3 | 4.7 | 2.0 | 2.1 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.33 | -0.54 | -0.63 | -0.66 | -0.83 | -0.79 | -0.86 | -0.87 | 0.07 | 0.05 | -0.09 | 0.92 | 0.97 | 0.94 | 0.11 | 0.14 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -3.2 | -3.6 | -1.8 | -3.0 | -3.6 | -1.7 | -2.0 | -1.0 | -3.0 | -4.5 | -5.5 | 0.3 | 0.5 | 0.1 |
Cytoplasm | 0.3 | -0.7 | -4.2 | -7.5 | -8.5 | -8.6 | -10.7 | -10.9 | -11.6 | 3.6 | 2.8 | 4.3 | 0.6 | 0.5 | 0.5 |
Endoplasmic Reticulum | -2.3 | -2.6 | -2.0 | -1.7 | -1.9 | -2.9 | -0.5 | -3.4 | -2.5 | -3.6 | -2.9 | -3.7 | -0.1 | 0.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 2.5 | 8.0 | 10.5 | 9.7 | 9.6 | 12.8 | 13.4 | 13.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.1 | 5.3 | 5.8 | 4.8 | 6.2 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.5895 | 8.3172 | 7.1529 | 5.9739 | 6.6452 | 7.312 | 6.7653 | 9.7179 | 8.8193 | 7.5691 | 7.5862 | 9.15 | 6.3341 | 9.4972 | 7.9604 | 6.3604 | 6.844 | 7.455 |
Actin | 0.0064 | 0.0073 | 0.0005 | 0.0001 | 0.0043 | 0.0004 | 0.0235 | 0.0016 | 0.0101 | 0.0093 | 0.0134 | 0.0016 | 0.0077 | 0.0009 | 0.0077 | 0.0314 | 0.0066 | 0.0016 |
Bud | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0281 | 0.0003 | 0.0012 | 0.0003 | 0.0006 | 0.0009 | 0.001 | 0.0004 | 0.0006 | 0.0027 | 0.0002 | 0.0012 | 0.0003 | 0.0013 |
Bud Neck | 0.0002 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0008 | 0.003 | 0.0003 | 0.0011 | 0.0007 | 0.0006 | 0.0008 | 0.0007 | 0.0002 | 0.0004 | 0.0012 | 0.0005 | 0.0021 |
Bud Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0146 | 0.0003 | 0.0013 | 0.0003 | 0.0005 | 0.0011 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0.0001 | 0.0015 | 0.0003 | 0.0007 |
Bud Site | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0057 | 0.0001 | 0.006 | 0.0015 | 0.0073 | 0.0045 | 0.0067 | 0.0003 | 0.0019 | 0.0034 | 0.0002 | 0.0052 | 0.0003 | 0.0005 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.9233 | 0.9722 | 0.9607 | 0.9139 | 0.915 | 0.9316 | 0.7255 | 0.9665 | 0.9344 | 0.8173 | 0.9001 | 0.9542 | 0.8849 | 0.982 | 0.9515 | 0.7923 | 0.8899 | 0.9466 |
Cytoplasmic Foci | 0.0141 | 0.0066 | 0.0091 | 0.0246 | 0.0129 | 0.0143 | 0.039 | 0.0075 | 0.0093 | 0.0147 | 0.0094 | 0.0112 | 0.0219 | 0.0029 | 0.0045 | 0.0218 | 0.0148 | 0.0118 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0068 | 0.0013 | 0.004 | 0.0017 | 0.0028 | 0.0037 | 0.0112 | 0.0015 | 0.0029 | 0.0097 | 0.0011 | 0.0034 | 0.0096 | 0.0011 | 0.0029 | 0.0027 | 0.0025 | 0.0036 |
Endosome | 0.019 | 0.0056 | 0.0175 | 0.0429 | 0.0086 | 0.0218 | 0.0796 | 0.0045 | 0.0091 | 0.0548 | 0.0108 | 0.012 | 0.0289 | 0.0017 | 0.0158 | 0.0409 | 0.0161 | 0.016 |
Golgi | 0.0026 | 0.0008 | 0.0014 | 0.0013 | 0.0005 | 0.0017 | 0.0209 | 0.0018 | 0.0021 | 0.0181 | 0.0072 | 0.0018 | 0.0071 | 0.0001 | 0.0078 | 0.0211 | 0.0098 | 0.0022 |
Lipid Particles | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0093 | 0.0004 | 0.0028 | 0.0038 | 0.0007 | 0.0013 | 0.0038 | 0 | 0.0014 | 0.0199 | 0.0051 | 0.0012 |
Mitochondria | 0.0005 | 0.0003 | 0.0004 | 0.0005 | 0.0007 | 0.009 | 0.0226 | 0.0078 | 0.0008 | 0.039 | 0.0407 | 0.0024 | 0.008 | 0.0001 | 0.003 | 0.0199 | 0.0187 | 0.0019 |
None | 0.0148 | 0.0017 | 0.0012 | 0.0075 | 0.0018 | 0.0106 | 0.0072 | 0.0012 | 0.0085 | 0.0025 | 0.0013 | 0.0013 | 0.0064 | 0.0022 | 0.0011 | 0.0013 | 0.0013 | 0.0012 |
Nuclear Periphery | 0.0014 | 0.0005 | 0.0006 | 0.0005 | 0.0007 | 0.0005 | 0.0093 | 0.0005 | 0.0009 | 0.008 | 0.0005 | 0.0015 | 0.0023 | 0.0003 | 0.0008 | 0.0053 | 0.0012 | 0.0014 |
Nucleolus | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0005 | 0 | 0 | 0.0005 | 0 | 0.0005 |
Nucleus | 0.0057 | 0.0009 | 0.0011 | 0.001 | 0.0012 | 0.0011 | 0.0115 | 0.0011 | 0.0015 | 0.0036 | 0.0019 | 0.0028 | 0.0043 | 0.0008 | 0.0009 | 0.0026 | 0.0018 | 0.0017 |
Peroxisomes | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.012 | 0.0017 | 0.0054 | 0.0013 | 0.0016 | 0.0012 | 0.0041 | 0 | 0.0004 | 0.0114 | 0.0217 | 0.0007 |
Punctate Nuclear | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0014 | 0.0067 | 0.0001 | 0.0007 | 0.0011 | 0.0003 | 0.0008 | 0.0014 | 0.0002 | 0.0002 | 0.0117 | 0.0068 | 0.0018 |
Vacuole | 0.0024 | 0.0011 | 0.0015 | 0.0036 | 0.0013 | 0.0015 | 0.0069 | 0.0009 | 0.0014 | 0.006 | 0.0013 | 0.0015 | 0.0038 | 0.0006 | 0.0007 | 0.0054 | 0.0018 | 0.0023 |
Vacuole Periphery | 0.0005 | 0.0003 | 0.0007 | 0.0011 | 0.0004 | 0.0007 | 0.0023 | 0.0002 | 0.0003 | 0.0029 | 0.0005 | 0.0006 | 0.001 | 0.0001 | 0.0004 | 0.0021 | 0.0007 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.5906 | 29.8128 | 32.8675 | 27.1797 | 41.3864 | 19.0465 | 21.3159 | 27.611 | 24.1223 | 32.0668 |
Translational Efficiency | 2.635 | 2.7202 | 2.2075 | 2.0362 | 1.7838 | 3.4716 | 2.2625 | 1.6345 | 1.9344 | 1.9817 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1559 | 189 | 1863 | 1318 | 1992 | 1952 | 241 | 1026 | 3551 | 2141 | 2104 | 2344 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1078.25 | 1249.61 | 1289.13 | 1398.54 | 1094.23 | 1201.74 | 1390.57 | 1491.47 | 1087.21 | 1205.97 | 1300.75 | 1439.22 |
Standard Deviation | 177.85 | 212.34 | 186.02 | 219.53 | 156.71 | 167.69 | 192.11 | 228.96 | 166.51 | 172.64 | 189.50 | 228.41 |
Intensity Change Log 2 | — | 0.212786 | 0.257706 | 0.375230 | — | 0.135209 | 0.345760 | 0.446819 | — | 0.174234 | 0.302729 | 0.411732 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000224 | 0.000717 | 0.000210 | 0.001400 | 0.000188 | 0.000245 | 0.001496 | 0.000726 | 0.000204 | 0.000287 | 0.000357 | 0.001105 |
Bud Neck | 0.000864 | 0.001074 | 0.001301 | 0.001410 | 0.000805 | 0.000591 | 0.003699 | 0.001570 | 0.000831 | 0.000634 | 0.001576 | 0.001480 |
Bud Site | 0.015755 | 0.034776 | 0.009715 | 0.036731 | 0.012476 | 0.012910 | 0.023365 | 0.023382 | 0.013916 | 0.014840 | 0.011278 | 0.030888 |
Cell Periphery | 0.000110 | 0.000217 | 0.000059 | 0.000779 | 0.000091 | 0.000074 | 0.000147 | 0.000374 | 0.000100 | 0.000087 | 0.000070 | 0.000602 |
Cytoplasm | 0.748544* | 0.725353* | 0.801792* | 0.848011* | 0.775391* | 0.791237* | 0.496259* | 0.830664* | 0.763604* | 0.785421* | 0.766795* | 0.840418* |
Cytoplasmic Foci | 0.165660 | 0.163968 | 0.056515 | 0.039230 | 0.146706 | 0.132014 | 0.109381 | 0.049964 | 0.155028 | 0.134835 | 0.062570 | 0.043929 |
Eisosomes | 0.000008 | 0.000008 | 0.000004 | 0.000010 | 0.000008 | 0.000006 | 0.000023 | 0.000006 | 0.000008 | 0.000006 | 0.000006 | 0.000008 |
Endoplasmic Reticulum | 0.002971 | 0.000693 | 0.001164 | 0.000546 | 0.003200 | 0.001554 | 0.003858 | 0.000368 | 0.003099 | 0.001478 | 0.001473 | 0.000468 |
Endosome | 0.008701 | 0.009744 | 0.007916 | 0.003443 | 0.006598 | 0.007984 | 0.027740 | 0.004661 | 0.007521 | 0.008140 | 0.010187 | 0.003977 |
Golgi | 0.010929 | 0.015769 | 0.001806 | 0.007426 | 0.008244 | 0.008596 | 0.001579 | 0.003908 | 0.009423 | 0.009229 | 0.001780 | 0.005886 |
Lipid Particles | 0.001306 | 0.002014 | 0.001401 | 0.007001 | 0.001013 | 0.000855 | 0.004240 | 0.003949 | 0.001142 | 0.000957 | 0.001726 | 0.005665 |
Mitochondria | 0.000717 | 0.000160 | 0.000119 | 0.000130 | 0.001330 | 0.000815 | 0.000047 | 0.000133 | 0.001061 | 0.000758 | 0.000110 | 0.000132 |
Mitotic Spindle | 0.000607 | 0.013128 | 0.011363 | 0.001997 | 0.001749 | 0.001817 | 0.008823 | 0.007863 | 0.001247 | 0.002815 | 0.011072 | 0.004565 |
None | 0.000650 | 0.000385 | 0.000501 | 0.000187 | 0.000893 | 0.000652 | 0.000733 | 0.000312 | 0.000786 | 0.000628 | 0.000528 | 0.000242 |
Nuclear Periphery | 0.000029 | 0.000015 | 0.000272 | 0.000041 | 0.000051 | 0.000027 | 0.000413 | 0.000047 | 0.000042 | 0.000026 | 0.000288 | 0.000043 |
Nuclear Periphery Foci | 0.000883 | 0.001111 | 0.002160 | 0.002024 | 0.000752 | 0.000676 | 0.008051 | 0.003085 | 0.000810 | 0.000715 | 0.002835 | 0.002489 |
Nucleolus | 0.000116 | 0.000057 | 0.000156 | 0.000130 | 0.000083 | 0.000069 | 0.000228 | 0.000162 | 0.000098 | 0.000068 | 0.000165 | 0.000144 |
Nucleus | 0.005660 | 0.003516 | 0.014820 | 0.004956 | 0.008040 | 0.005655 | 0.015229 | 0.006258 | 0.006996 | 0.005466 | 0.014867 | 0.005526 |
Peroxisomes | 0.000503 | 0.000934 | 0.000341 | 0.000828 | 0.000407 | 0.000586 | 0.001485 | 0.000750 | 0.000449 | 0.000617 | 0.000472 | 0.000794 |
Vacuole | 0.030374 | 0.023524 | 0.085619 | 0.041315 | 0.024247 | 0.030126 | 0.290951* | 0.059556 | 0.026937 | 0.029543 | 0.109138 | 0.049300 |
Vacuole Periphery | 0.005387 | 0.002835 | 0.002766 | 0.002405 | 0.007728 | 0.003509 | 0.002253 | 0.002261 | 0.006701 | 0.003450 | 0.002708 | 0.002342 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.51 | -0.38 | -3.52 | -1.44 | -3.48 | -1.59 | -1.42 | -5.32 | -4.58 | 0.66 | -1.91 | -1.67 | -4.75 | -4.21 | -3.60 |
Bud Neck | -1.30 | -6.47 | -5.44 | -2.38 | 0.72 | 2.42 | -6.68 | -7.25 | -10.22 | 4.21 | 3.01 | -9.42 | -8.94 | -12.88 | 1.54 |
Bud Site | -4.75 | 4.67 | -9.28 | -0.30 | -12.42 | -0.34 | -3.34 | -7.24 | -6.78 | 0.20 | -0.78 | 2.27 | -11.94 | -10.26 | -12.89 |
Cell Periphery | -3.08 | 7.16 | -10.45 | -7.65 | -11.25 | 2.59 | -4.02 | -4.94 | -5.31 | -3.89 | 2.20 | 5.24 | -11.45 | -11.76 | -12.13 |
Cytoplasm | 1.20 | -4.10 | -11.73 | -6.06 | -7.78 | -2.15 | 13.62 | -3.54 | -1.61 | -14.85 | -3.41 | 1.85 | -10.94 | -6.18 | -10.62 |
Cytoplasmic Foci | 0.34 | 21.55 | 25.58 | 9.83 | 7.08 | 2.75 | 6.45 | 21.51 | 18.70 | 7.97 | 4.41 | 26.34 | 33.76 | 23.60 | 7.87 |
Eisosomes | -0.10 | 7.61 | -0.89 | -0.72 | -2.75 | 2.75 | -6.35 | 1.80 | -0.87 | 6.99 | 3.29 | 2.62 | -0.30 | -1.89 | -1.49 |
Endoplasmic Reticulum | 5.38 | 4.23 | 5.93 | 1.26 | 3.01 | 2.79 | 0.04 | 5.53 | 4.09 | 3.25 | 3.77 | 4.58 | 7.77 | 3.89 | 4.60 |
Endosome | -0.30 | 0.97 | 8.26 | 2.58 | 8.99 | -2.00 | -7.40 | 3.68 | 5.35 | 8.41 | -0.89 | -3.76 | 8.65 | 7.58 | 11.14 |
Golgi | -1.05 | 20.12 | 4.21 | 2.03 | -5.63 | -0.50 | 17.24 | 8.54 | 7.59 | -3.20 | 0.41 | 26.49 | 7.00 | 4.95 | -6.27 |
Lipid Particles | -1.28 | -2.40 | -8.86 | -5.83 | -8.40 | 1.41 | -5.94 | -8.56 | -9.01 | -0.73 | 1.69 | -6.42 | -11.71 | -12.05 | -9.74 |
Mitochondria | 1.58 | 1.70 | 1.66 | 0.43 | -0.22 | 0.87 | 3.04 | 2.88 | 1.66 | -1.29 | 0.64 | 3.36 | 3.31 | 1.64 | -0.23 |
Mitotic Spindle | -1.76 | -9.74 | -2.09 | 1.49 | 6.78 | -0.12 | -2.14 | -2.59 | -2.54 | 0.67 | -2.08 | -9.36 | -3.12 | -1.23 | 5.44 |
None | 1.58 | 1.50 | 5.00 | 1.56 | 7.31 | 1.39 | 0.18 | 3.81 | 2.64 | 1.68 | 1.19 | 2.50 | 5.90 | 3.70 | 5.02 |
Nuclear Periphery | 1.72 | -10.25 | -0.90 | -1.23 | 6.69 | 2.07 | -4.19 | -0.80 | -4.97 | 4.09 | 2.22 | -10.48 | -0.94 | -1.97 | 8.75 |
Nuclear Periphery Foci | -1.24 | -6.68 | -7.02 | -2.38 | 0.94 | 0.32 | -7.01 | -6.73 | -7.20 | 4.35 | 0.48 | -9.38 | -9.32 | -9.86 | 1.37 |
Nucleolus | 2.67 | -2.62 | -0.45 | -1.15 | 0.24 | 2.19 | -3.54 | -1.04 | -1.21 | 0.61 | 3.11 | -5.54 | -1.04 | -1.54 | 0.35 |
Nucleus | 1.85 | -15.46 | -1.60 | -2.68 | 12.44 | 4.30 | -4.56 | -0.06 | -4.32 | 4.53 | 3.25 | -14.43 | -0.52 | -3.39 | 13.49 |
Peroxisomes | -3.18 | 3.59 | -3.58 | 1.10 | -5.36 | -1.29 | -3.00 | -4.46 | -0.89 | 1.93 | -1.37 | -0.31 | -5.61 | -1.06 | -4.49 |
Vacuole | 0.64 | -15.94 | -6.52 | -4.44 | 10.65 | -3.33 | -16.05 | -14.57 | -12.87 | 12.02 | -1.95 | -21.66 | -15.54 | -13.26 | 11.63 |
Vacuole Periphery | 3.18 | 4.94 | 5.07 | 0.72 | 0.61 | 4.85 | 7.16 | 6.61 | 3.02 | 0.27 | 5.38 | 7.84 | 8.26 | 3.25 | 1.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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