Standard name
Human Ortholog
Description Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.12 0.11 0.13 0.13 0.11 0.16 – 0.11 0.13 0.12 0 0 0.05 0.12 0.11 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0.05 0.07 0.08 0.07 0.09 0.06 – 0.09 0.13 0.13 0.1 0.1 0.21 0.37 0.36 0.38 0 0 0 0 0 0
Cytoplasm 0.09 0.16 0.13 0.18 0.16 0.22 0.17 0.27 – 0.28 0.29 0.22 0.16 0.06 0.07 0.44 0.38 0.44 0 0 0 0 0 0
Endoplasmic Reticulum 0.91 0.87 0.82 0.75 0.71 0.64 0.74 0.38 – 0.56 0.34 0.47 0.81 0.9 0.88 0 0 0 0.98 0.91 0.88 0.91 0.92 0.87
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0.12 0 0 0 0 – 0 0 0 0 0 0 0.15 0.17 0.09 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0 0.05 0 – 0.05 0.25 0.17 0 0 0 0 0.07 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.05 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.09 0 0.19 – 0.07 0.08 0.08 0 0 0 0 0.05 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 13 14 27 22 32 39 – 33 30 36 1 5 10 16 10 7 0 2 1 2 2 3
Bud 1 0 1 0 9 11 3 11 – 5 16 12 0 1 0 2 2 1 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 – – – – – –
Cell Periphery 8 9 5 9 17 13 26 15 – 27 30 39 9 16 42 51 33 39 0 0 1 0 0 1
Cytoplasm 20 26 14 22 33 38 49 67 – 87 64 65 14 9 13 61 35 45 0 4 2 2 4 6
Endoplasmic Reticulum 203 141 85 93 149 112 215 95 – 171 76 135 73 140 174 3 1 2 222 152 99 152 174 156
Endosome 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0
Golgi 2 1 3 15 4 0 0 3 – 1 0 1 1 3 1 21 16 9 0 0 0 0 1 0
Mitochondria 0 1 2 7 0 1 14 10 – 15 56 49 0 0 0 5 6 2 0 0 5 2 2 1
Nucleus 0 0 0 0 0 1 2 0 – 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Nuclear Periphery 2 0 3 4 0 2 14 3 – 14 1 10 0 1 2 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 – 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 3 3 3 18 15 6 47 – 22 18 22 1 0 0 2 5 5 1 2 0 0 2 5
Unique Cell Count 223 163 104 124 211 175 291 248 307 224 290 90 156 197 139 92 103 228 168 113 167 191 180
Labelled Cell Count 240 184 129 167 258 216 361 291 378 292 370 99 175 243 162 109 111 228 168 113 167 191 180


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.8 25.0 15.2 15.6 15.4 13.2 11.7 12.3 – 12.5 11.4 11.5 23.2 22.9 23.1 24.2 24.0 24.4 23.2 25.7 25.7
Std Deviation (1e-4) 4.1 4.7 2.8 3.2 2.5 2.4 2.2 2.2 – 2.9 2.7 2.2 4.3 4.3 4.2 5.2 5.1 5.4 5.2 5.7 5.6
Intensity Change (Log2) – – – 0.04 0.02 -0.2 -0.38 -0.3 – -0.28 -0.42 -0.4 0.61 0.59 0.61 0.67 0.66 0.68 0.61 0.76 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.3 0.1 0 -0.4 0.8 – -0.5 0.2 0 -3.1 -2.9 -2.3 -0.2 -0.4 -1.4
Bud 0 0 0 0 0 – 0 2.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 1.1 0.9 1.3 0.5 – 1.3 2.3 2.4 1.4 1.6 3.8 5.8 5.5 5.8
Cytoplasm 0.9 0.5 1.7 0.8 2.8 – 3.0 3.0 2.0 0.4 -2.1 -2.0 5.1 4.0 4.8
Endoplasmic Reticulum -1.2 -2.1 -3.1 -1.6 -7.4 – -4.7 -8.1 -6.2 -0.1 1.9 1.6 -12.8 -11.4 -11.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 2.6 0 0 0 0 – 0 0 0 0 0 0 3.2 3.4 1.8
Mitochondria 0 0 0 0 0 – 0 5.1 3.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 1.9 0 3.9 – 1.6 1.8 1.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 21.3478 25.3865 23.9412 23.9337 20.1972 24.1409 29.1091 29.3205 28.3464 24.2719 25.8915 28.8271 27.9792 27.9827 28.0328 26.2323 25.3836 26.837
Actin 0.0189 0.0003 0.0027 0.0057 0.0039 0.0054 0.0082 0.0004 0.0008 0.001 0.0005 0 0.001 0 0 0 0 0.0003
Bud 0.0004 0.0001 0.0002 0.0003 0.0003 0 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0
Bud Neck 0.0005 0.0001 0.0001 0.0002 0.0054 0.0003 0.0002 0.0005 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0004
Bud Periphery 0.0006 0.0002 0.0011 0.0007 0.0036 0.0001 0.0001 0.0002 0.0001 0.0012 0.0002 0 0.0001 0.0004 0 0 0 0
Bud Site 0.0013 0.005 0.0004 0.0003 0.0001 0 0.0003 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Cell Periphery 0.0104 0.0104 0.0073 0.0084 0.0232 0.0022 0.002 0.0076 0.0042 0.004 0.0029 0.0009 0.0066 0.0189 0.0035 0.0045 0.002 0.0012
Cytoplasm 0.0075 0.008 0.0022 0.001 0.0022 0.0056 0.0018 0.0244 0.0024 0.0006 0.0026 0.0005 0.0033 0.0017 0.0006 0.0008 0.0006 0.001
Cytoplasmic Foci 0.0102 0.0003 0.0003 0.0024 0.0076 0.001 0.0024 0.0005 0.0002 0 0.0014 0 0.0008 0 0 0 0 0
Eisosomes 0.0009 0 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0003 0.0016 0.0003 0 0.0002 0.0005 0 0 0 0
Endoplasmic Reticulum 0.821 0.935 0.9613 0.9308 0.7759 0.9546 0.9216 0.9385 0.975 0.9656 0.9585 0.9849 0.9367 0.9632 0.988 0.9804 0.9882 0.9798
Endosome 0.0153 0.0005 0.0019 0.0082 0.027 0.0038 0.0052 0.0013 0.0005 0.0003 0.0068 0.0002 0.0046 0.0001 0.0001 0.0002 0.0002 0.0002
Golgi 0.0096 0.0002 0.0012 0.0052 0.0113 0.0019 0.003 0.0016 0.0005 0.0002 0.0026 0.0001 0.001 0 0.0001 0.0002 0 0.0001
Lipid Particles 0.0165 0.0081 0.0014 0.0088 0.0185 0.0032 0.0067 0.0069 0.0028 0.0005 0.0066 0.0002 0.0037 0.0041 0.0007 0.0006 0.0003 0.0009
Mitochondria 0.0157 0.0003 0.0045 0.0044 0.0103 0.0012 0.0047 0.0094 0.0045 0.0172 0.0033 0 0.0015 0.0001 0 0 0 0
None 0.0013 0.0012 0.0003 0.0002 0.0002 0.0002 0.0013 0.0001 0.0001 0.0001 0.0017 0 0.0013 0 0 0 0 0
Nuclear Periphery 0.0467 0.0188 0.0088 0.0143 0.0333 0.0129 0.0311 0.0045 0.0052 0.0044 0.0079 0.0108 0.031 0.0075 0.005 0.0108 0.0068 0.0133
Nucleolus 0.0003 0.0035 0.0006 0 0.0002 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Nucleus 0.0027 0.0031 0.0005 0.0004 0.0018 0.0005 0.0008 0.0002 0.0001 0.0001 0.0002 0.0004 0.0011 0.0001 0 0.0003 0.0002 0.0004
Peroxisomes 0.003 0 0.0001 0.0002 0.0491 0.0009 0.002 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0027 0.0005 0 0 0.0002 0.0002 0.0013 0.0001 0 0 0.0001 0 0.0005 0 0 0 0 0
Vacuole 0.0119 0.0037 0.0046 0.0052 0.0191 0.005 0.0052 0.0033 0.0027 0.0021 0.0028 0.0017 0.0044 0.0031 0.0019 0.0018 0.0014 0.002
Vacuole Periphery 0.0028 0.0006 0.0005 0.0028 0.0064 0.001 0.0018 0.0004 0.0002 0.0005 0.0014 0.0003 0.0017 0.0001 0.0001 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 87.7228 70.8604 82.0271 109.671 89.7558 84.5053 96.0068 110.8982 98.948 116.7069
Translational Efficiency 1.628 1.7371 1.4538 1.1827 1.3227 1.574 1.235 1.1028 1.2005 1.0325

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1973 1342 212 302 1886 1658 2450 1754 3859 3000 2662 2056

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1391.04 1719.42 2434.07 1685.40 1551.64 1808.17 2299.65 2353.25 1469.53 1768.47 2310.36 2255.15
Standard Deviation 244.21 395.16 370.75 609.59 368.37 347.41 435.70 544.51 321.33 372.16 432.42 602.84
Intensity Change Log 2 — 0.305758 0.807207 0.276927 — 0.220736 0.567620 0.600861 — 0.261552 0.685844 0.456742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000807 0.000428 0.000175 0.000922 0.000433 0.000859 0.000023 0.000167 0.000624 0.000666 0.000035 0.000278
Bud Neck 0.000147 0.000348 0.003063 0.003495 0.000496 0.000171 0.000050 0.000565 0.000318 0.000250 0.000290 0.000996
Bud Site 0.001111 0.002195 0.001133 0.013501 0.004586 0.001963 0.000181 0.000943 0.002809 0.002067 0.000257 0.002788
Cell Periphery 0.002183 0.003249 0.015924 0.011356 0.004498 0.002751 0.002674 0.007180 0.003314 0.002974 0.003729 0.007793
Cytoplasm 0.002564 0.003464 0.000089 0.040140 0.007587 0.001426 0.000768 0.003955 0.005019 0.002338 0.000714 0.009270
Cytoplasmic Foci 0.001409 0.002553 0.000028 0.007116 0.008425 0.001577 0.000222 0.000546 0.004838 0.002014 0.000207 0.001511
Eisosomes 0.000030 0.000041 0.000302 0.000126 0.000063 0.000084 0.000027 0.000090 0.000046 0.000065 0.000049 0.000095
Endoplasmic Reticulum 0.979935* 0.947132* 0.918484* 0.664358* 0.912772* 0.976164* 0.991821* 0.945649* 0.947110* 0.963177* 0.985980* 0.904331*
Endosome 0.003084 0.011224 0.005624 0.023507 0.015190 0.004538 0.000945 0.006190 0.009000 0.007529 0.001318 0.008733
Golgi 0.001125 0.005092 0.003363 0.036898 0.010286 0.002142 0.000292 0.004487 0.005602 0.003461 0.000537 0.009247
Lipid Particles 0.002134 0.008462 0.005246 0.020151 0.010204 0.003156 0.000501 0.006504 0.006078 0.005529 0.000879 0.008508
Mitochondria 0.000369 0.001004 0.000088 0.000279 0.000877 0.000505 0.000148 0.000191 0.000617 0.000728 0.000143 0.000204
Mitotic Spindle 0.000041 0.000208 0.000028 0.001322 0.000180 0.000051 0.000007 0.000034 0.000109 0.000121 0.000009 0.000223
None 0.001623 0.001095 0.000008 0.000459 0.001628 0.000483 0.000578 0.000998 0.001626 0.000757 0.000533 0.000919
Nuclear Periphery 0.000241 0.000694 0.006684 0.004934 0.000748 0.000213 0.000324 0.001491 0.000488 0.000428 0.000831 0.001997
Nuclear Periphery Foci 0.001342 0.006436 0.009624 0.092221 0.009770 0.000971 0.000184 0.008046 0.005461 0.003416 0.000936 0.020410
Nucleolus 0.000007 0.000025 0.000005 0.000149 0.000048 0.000005 0.000001 0.000016 0.000027 0.000014 0.000001 0.000035
Nucleus 0.000115 0.000152 0.000068 0.002309 0.000222 0.000050 0.000022 0.000176 0.000167 0.000096 0.000026 0.000489
Peroxisomes 0.000280 0.000124 0.000003 0.000056 0.000364 0.000177 0.000080 0.000012 0.000321 0.000153 0.000074 0.000019
Vacuole 0.000909 0.004519 0.027533 0.063890 0.008238 0.001777 0.001055 0.010924 0.004491 0.003004 0.003164 0.018704
Vacuole Periphery 0.000546 0.001552 0.002524 0.012812 0.003385 0.000938 0.000094 0.001837 0.001933 0.001213 0.000288 0.003449

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.70 1.17 -0.27 -2.16 -3.38 -1.15 4.96 1.85 1.77 -1.74 -0.13 2.17 1.11 1.60 -2.99
Bud Neck -2.86 -2.90 -5.67 -5.35 -0.02 4.30 6.66 -0.99 -4.12 -5.55 1.32 -0.21 -5.63 -6.22 -4.49
Bud Site -1.73 -0.40 -4.53 -4.13 -4.28 3.31 7.05 5.45 1.75 -3.70 1.45 6.61 -0.18 -1.47 -5.59
Cell Periphery -1.60 -5.29 -4.45 -3.84 1.37 3.55 3.66 -3.89 -6.84 -6.91 0.85 -0.95 -7.06 -7.40 -6.06
Cytoplasm -1.39 6.82 -5.32 -5.18 -5.67 7.39 8.30 3.22 -3.41 -4.31 5.08 9.18 -3.37 -5.51 -6.86
Cytoplasmic Foci -1.79 4.31 -4.38 -3.29 -5.63 6.04 7.81 7.44 1.99 -1.15 4.38 8.25 5.55 1.13 -4.07
Eisosomes -1.04 -2.95 -2.99 -2.61 1.81 -0.94 3.75 -1.22 -0.21 -2.93 -1.42 -0.26 -2.58 -1.39 -2.28
Endoplasmic Reticulum 7.19 5.63 14.21 12.58 10.13 -12.27 -16.97 -5.29 7.41 12.82 -4.72 -13.87 7.98 11.00 16.08
Endosome -8.64 -3.61 -9.95 -5.53 -8.26 10.50 15.60 8.99 -2.95 -13.39 2.19 15.90 0.24 -1.96 -15.68
Golgi -4.86 -4.74 -6.36 -5.59 -5.94 6.53 8.57 4.51 -3.46 -8.04 2.93 8.61 -3.22 -5.46 -8.99
Lipid Particles -4.60 -1.42 -5.65 -3.44 -3.59 6.25 10.01 2.61 -3.06 -6.24 0.62 8.78 -2.34 -2.66 -7.82
Mitochondria -1.13 3.23 0.78 1.27 -3.41 0.76 1.63 1.47 1.51 -1.04 -0.34 2.08 1.73 1.83 -1.39
Mitotic Spindle -1.67 0.78 -1.21 -1.05 -1.22 4.11 6.03 5.05 1.12 -5.02 -0.25 6.37 -0.73 -0.63 -1.38
None 0.90 3.61 2.52 1.61 -4.44 2.49 2.42 1.06 -1.11 -0.96 2.38 3.34 1.49 -0.40 -1.03
Nuclear Periphery -3.95 -6.84 -4.94 -4.45 1.23 3.49 2.77 -4.07 -11.87 -10.82 0.68 -2.94 -8.16 -9.02 -6.29
Nuclear Periphery Foci -4.98 -4.57 -8.64 -8.12 -7.69 8.12 8.93 0.97 -6.41 -7.15 2.89 7.90 -7.55 -8.66 -10.11
Nucleolus -1.02 0.47 -2.79 -2.29 -2.81 2.25 2.49 1.63 -2.75 -4.49 1.04 2.74 -0.75 -1.93 -4.29
Nucleus -0.56 0.78 -4.02 -3.95 -4.09 5.47 6.59 0.05 -2.75 -3.23 2.00 5.19 -3.62 -4.34 -5.15
Peroxisomes 1.79 3.70 2.93 1.51 -3.58 2.04 3.53 4.47 3.50 4.14 2.65 4.38 5.57 4.16 3.63
Vacuole -6.07 -5.02 -9.38 -8.82 -4.13 6.53 7.85 -2.22 -7.81 -8.79 2.66 1.84 -9.63 -10.80 -10.35
Vacuole Periphery -2.24 -1.47 -5.25 -4.75 -3.80 4.15 7.34 2.82 -1.99 -6.29 1.97 6.41 -3.19 -4.43 -7.25
Standard name
Human Ortholog
Description Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Ifa38

Ifa38


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ifa38-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available