Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 30 | 4 | 14 | 23 | 13 | 16 | – | 19 | 7 | 19 | 19 | 38 | 22 | 28 | 17 | 27 | 23 | 15 | 30 | 5 | 19 | 13 | 33 | 33 |
Bud | 5 | 2 | 10 | 15 | 8 | 14 | – | 22 | 19 | 25 | 16 | 20 | 3 | 3 | 1 | 2 | 3 | 0 | 6 | 1 | 3 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 0 | 2 | 1 | 1 | 1 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 5 | 9 | 10 | 8 | – | 17 | 13 | 19 | 30 | 45 | 7 | 16 | 25 | 12 | 7 | 19 | 3 | 0 | 2 | 0 | 3 | 9 |
Cytoplasm | 122 | 32 | 58 | 39 | 41 | 54 | – | 78 | 29 | 38 | 44 | 49 | 71 | 78 | 136 | 61 | 43 | 38 | 34 | 14 | 6 | 0 | 3 | 7 |
Endoplasmic Reticulum | 218 | 30 | 83 | 89 | 103 | 162 | – | 130 | 109 | 139 | 122 | 179 | 228 | 174 | 137 | 3 | 3 | 4 | 313 | 70 | 137 | 81 | 172 | 212 |
Endosome | 10 | 0 | 0 | 2 | 30 | 10 | – | 18 | 1 | 5 | 1 | 2 | 10 | 11 | 8 | 1 | 10 | 2 | 12 | 3 | 3 | 5 | 9 | 10 |
Golgi | 2 | 0 | 2 | 2 | 17 | 1 | – | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 2 | 9 | 8 | 3 | 7 | 0 | 3 | 7 | 18 | 17 |
Mitochondria | 40 | 5 | 82 | 211 | 104 | 290 | – | 306 | 265 | 296 | 282 | 403 | 2 | 4 | 3 | 6 | 15 | 6 | 46 | 8 | 55 | 6 | 19 | 18 |
Nucleus | 1 | 4 | 0 | 0 | 0 | 2 | – | 4 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 1 | 4 | – | 9 | 10 | 9 | 5 | 13 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 0 | 0 | 1 | 0 | – | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 1 | 1 | – | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 40 | 1 | 10 | 5 | 29 | 31 | – | 95 | 49 | 41 | 19 | 23 | 5 | 5 | 6 | 14 | 6 | 5 | 22 | 6 | 13 | 7 | 14 | 13 |
Unique Cell Count | 403 | 70 | 206 | 285 | 283 | 458 | 548 | 366 | 436 | 385 | 550 | 309 | 274 | 292 | 122 | 109 | 86 | 497 | 116 | 256 | 127 | 286 | 335 | |
Labelled Cell Count | 476 | 81 | 266 | 397 | 358 | 593 | 703 | 504 | 595 | 541 | 778 | 350 | 320 | 337 | 139 | 125 | 100 | 497 | 116 | 256 | 127 | 286 | 335 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 6.2 | 5.1 | 6.4 | 7.1 | 5.7 | – | 6.0 | 5.3 | 5.8 | 5.8 | 6.0 | 7.3 | 7.4 | 7.5 | 9.8 | 9.2 | 9.1 | 8.4 | 9.0 | 9.1 |
Std Deviation (1e-4) | 0.9 | 1.1 | 1.2 | 1.4 | 1.6 | 1.0 | – | 1.4 | 1.1 | 1.2 | 1.5 | 1.6 | 1.1 | 1.3 | 1.3 | 2.9 | 2.3 | 2.2 | 1.5 | 1.8 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.32 | 0.47 | 0.16 | – | 0.23 | 0.07 | 0.17 | 0.19 | 0.23 | 0.51 | 0.54 | 0.55 | 0.94 | 0.84 | 0.84 | 0.71 | 0.82 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -1.1 | -1.9 | – | -2.0 | -3.0 | -1.3 | -0.9 | 0.1 | 0.1 | 1.3 | -0.4 | 4.1 | 3.8 | 2.8 |
Bud | 0.2 | -1.2 | -1.1 | – | -0.5 | 0.2 | 0.5 | -0.4 | -0.8 | -2.8 | -2.5 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | 0.7 | 0 | – | 0.5 | 0.7 | 1.2 | 2.6 | 2.8 | 0 | 1.8 | 2.8 | 2.9 | 0 | 5.6 |
Cytoplasm | -4.0 | -3.7 | -5.2 | – | -4.4 | -6.5 | -6.4 | -5.1 | -6.8 | -1.3 | 0.1 | 4.2 | 4.0 | 2.0 | 2.7 |
Endoplasmic Reticulum | -2.1 | -0.9 | -1.2 | – | -4.5 | -2.6 | -2.1 | -2.1 | -2.0 | 7.6 | 5.0 | 1.5 | -7.5 | -7.1 | -6.1 |
Endosome | 0 | 4.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 2.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.6 | -0.7 | 5.6 | – | 3.9 | 7.7 | 6.8 | 8.0 | 8.5 | -11.8 | -10.8 | -11.3 | -6.9 | -4.8 | -5.6 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.0 | 2.2 | 0.9 | – | 4.4 | 3.2 | 2.0 | 0 | -0.4 | -2.1 | -1.9 | -1.7 | 2.2 | 0.3 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6219 | 6.3723 | 6.2596 | 5.6007 | 5.2887 | 6.1214 | 6.2067 | 5.6402 | 5.6865 | 5.5592 | 5.4911 | 5.6551 | 9.6982 | 8.8672 | 8.0943 | 7.6737 | 6.5147 | 8.4968 |
Actin | 0.033 | 0.0082 | 0.0199 | 0.0167 | 0.0144 | 0.0046 | 0.0239 | 0.0293 | 0.0216 | 0.0038 | 0.0095 | 0.0067 | 0.0383 | 0.0071 | 0.0103 | 0.0034 | 0.0886 | 0.0118 |
Bud | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0009 | 0.0005 | 0.0017 | 0.0061 | 0.001 | 0.0017 | 0.0006 | 0.0011 | 0.0016 | 0.0039 | 0.001 | 0.0022 | 0.0017 | 0.0008 | 0.0006 | 0.0004 | 0.0009 | 0.0042 |
Bud Periphery | 0.0006 | 0.0009 | 0.0006 | 0.0021 | 0.0002 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0001 | 0.0012 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0006 | 0.0005 | 0.0003 |
Bud Site | 0.0048 | 0.0018 | 0.0024 | 0.0012 | 0.0003 | 0.0002 | 0.0046 | 0.0025 | 0.0066 | 0.0002 | 0.0023 | 0.0003 | 0.0016 | 0.002 | 0.0004 | 0.0001 | 0.0016 | 0.0002 |
Cell Periphery | 0.0026 | 0.0094 | 0.0033 | 0.0133 | 0.0026 | 0.0017 | 0.0044 | 0.0062 | 0.0022 | 0.0017 | 0.0026 | 0.0021 | 0.0016 | 0.0097 | 0.0051 | 0.009 | 0.0011 | 0.0016 |
Cytoplasm | 0.0205 | 0.0542 | 0.0239 | 0.0171 | 0.0412 | 0.0367 | 0.0317 | 0.108 | 0.051 | 0.0825 | 0.0159 | 0.0209 | 0.0364 | 0.0397 | 0.0552 | 0.0062 | 0.0039 | 0.0215 |
Cytoplasmic Foci | 0.0149 | 0.022 | 0.0108 | 0.0217 | 0.0126 | 0.0113 | 0.0172 | 0.0242 | 0.0128 | 0.0061 | 0.0166 | 0.0157 | 0.0153 | 0.0116 | 0.0099 | 0.0029 | 0.0121 | 0.0136 |
Eisosomes | 0.0007 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0002 | 0.0005 | 0.0011 | 0.0002 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.001 | 0.0003 | 0.0012 | 0.0008 | 0.0002 |
Endoplasmic Reticulum | 0.7044 | 0.7154 | 0.7917 | 0.7082 | 0.7423 | 0.7875 | 0.7161 | 0.5941 | 0.7758 | 0.8113 | 0.7623 | 0.8122 | 0.7444 | 0.7387 | 0.7774 | 0.8633 | 0.5994 | 0.7726 |
Endosome | 0.0702 | 0.0653 | 0.0402 | 0.054 | 0.0839 | 0.0603 | 0.0588 | 0.0838 | 0.0437 | 0.0391 | 0.0569 | 0.0535 | 0.0448 | 0.0641 | 0.0425 | 0.0308 | 0.1086 | 0.0572 |
Golgi | 0.037 | 0.0422 | 0.0297 | 0.0439 | 0.0417 | 0.0364 | 0.0269 | 0.0565 | 0.0261 | 0.0067 | 0.0662 | 0.0364 | 0.0432 | 0.0347 | 0.0394 | 0.0501 | 0.0614 | 0.0623 |
Lipid Particles | 0.0429 | 0.0456 | 0.0269 | 0.0416 | 0.0221 | 0.0227 | 0.0223 | 0.0561 | 0.0272 | 0.0103 | 0.0416 | 0.0188 | 0.0307 | 0.0622 | 0.0354 | 0.0055 | 0.0513 | 0.0258 |
Mitochondria | 0.0283 | 0.0123 | 0.0158 | 0.0372 | 0.0035 | 0.012 | 0.0141 | 0.0123 | 0.0056 | 0.0016 | 0.0063 | 0.0071 | 0.0066 | 0.0057 | 0.0073 | 0.0143 | 0.026 | 0.0101 |
None | 0.0032 | 0.0012 | 0.0009 | 0.0021 | 0.0036 | 0.0012 | 0.0086 | 0.002 | 0.0009 | 0.0145 | 0.0006 | 0.0017 | 0.0012 | 0.0009 | 0.0007 | 0.0008 | 0.0022 | 0.0004 |
Nuclear Periphery | 0.0213 | 0.0083 | 0.0084 | 0.0072 | 0.0173 | 0.0122 | 0.043 | 0.0098 | 0.012 | 0.0092 | 0.0055 | 0.0098 | 0.0216 | 0.0107 | 0.008 | 0.0065 | 0.012 | 0.0091 |
Nucleolus | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 |
Nucleus | 0.0012 | 0.001 | 0.0011 | 0.0013 | 0.0015 | 0.001 | 0.0056 | 0.001 | 0.0009 | 0.0009 | 0.0005 | 0.0006 | 0.0013 | 0.0007 | 0.0004 | 0.0003 | 0.0008 | 0.0008 |
Peroxisomes | 0.0015 | 0.001 | 0.0108 | 0.0148 | 0.0005 | 0.0017 | 0.0057 | 0.0008 | 0.0048 | 0.0007 | 0.0037 | 0.0012 | 0.0032 | 0.0002 | 0.0002 | 0.0001 | 0.0152 | 0.0016 |
Punctate Nuclear | 0.0027 | 0.0004 | 0.0043 | 0.0024 | 0.0016 | 0.0004 | 0.0043 | 0.0007 | 0.0009 | 0.0013 | 0.0011 | 0.002 | 0.0015 | 0.0003 | 0.0002 | 0.0001 | 0.0044 | 0.0003 |
Vacuole | 0.0059 | 0.0079 | 0.0051 | 0.0057 | 0.0061 | 0.0054 | 0.0064 | 0.0072 | 0.0042 | 0.0041 | 0.003 | 0.0059 | 0.004 | 0.0078 | 0.0047 | 0.0029 | 0.0052 | 0.0042 |
Vacuole Periphery | 0.0031 | 0.0016 | 0.0017 | 0.0019 | 0.003 | 0.0023 | 0.0038 | 0.0021 | 0.0013 | 0.0014 | 0.0015 | 0.002 | 0.0016 | 0.0014 | 0.0014 | 0.0017 | 0.0035 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 119.8141 | 174.3594 | 158.9553 | 172.4782 | 245.6607 | 133.672 | 163.7022 | 186.9796 | 177.4578 | 196.4304 |
Translational Efficiency | 3.2203 | 2.4378 | 1.8907 | 2.4008 | 1.9154 | 2.7642 | 1.7595 | 1.7052 | 1.8148 | 1.6317 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
17 | 871 | 387 | 1045 | 1713 | 1626 | 2268 | 1457 | 1730 | 2497 | 2655 | 2502 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1354.55 | 860.25 | 1110.31 | 1244.80 | 796.58 | 854.91 | 1122.41 | 1229.54 | 802.06 | 856.77 | 1120.65 | 1235.91 |
Standard Deviation | 232.36 | 124.46 | 148.68 | 206.92 | 111.91 | 125.52 | 170.04 | 194.05 | 126.33 | 125.18 | 167.15 | 199.67 |
Intensity Change Log 2 | — | -0.654986 | -0.286851 | -0.121900 | — | 0.101953 | 0.494709 | 0.626228 | — | -0.326752 | 0.053708 | 0.201949 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.043899 | 0.004320 | 0.008286 | 0.021928 | 0.002883 | 0.004813 | 0.011847 | 0.021675 | 0.003286 | 0.004641 | 0.011328 | 0.021781 |
Bud Neck | 0.004306 | 0.002555 | 0.002856 | 0.005153 | 0.001993 | 0.002664 | 0.000953 | 0.006599 | 0.002016 | 0.002626 | 0.001230 | 0.005995 |
Bud Site | 0.051377 | 0.007631 | 0.022233 | 0.039848 | 0.004225 | 0.004478 | 0.007322 | 0.038415 | 0.004689 | 0.005578 | 0.009496 | 0.039013 |
Cell Periphery | 0.004977 | 0.001476 | 0.000802 | 0.004163 | 0.001526 | 0.001800 | 0.003282 | 0.004123 | 0.001560 | 0.001687 | 0.002920 | 0.004140 |
Cytoplasm | 0.087822 | 0.092978 | 0.108618* | 0.036444 | 0.139510* | 0.124214* | 0.022838 | 0.051192 | 0.139002* | 0.113318* | 0.035342 | 0.045032 |
Cytoplasmic Foci | 0.064015 | 0.103127 | 0.052862 | 0.046821 | 0.070969 | 0.095364 | 0.015277 | 0.037363 | 0.070901 | 0.098071 | 0.020756 | 0.041313 |
Eisosomes | 0.000666 | 0.001691 | 0.000239 | 0.000841 | 0.002656 | 0.002640 | 0.001091 | 0.001271 | 0.002637 | 0.002309 | 0.000967 | 0.001091 |
Endoplasmic Reticulum | 0.416192* | 0.442303* | 0.579289* | 0.624687* | 0.462511* | 0.391581* | 0.824020* | 0.626764* | 0.462056* | 0.409274* | 0.788347* | 0.625896* |
Endosome | 0.068237 | 0.030774 | 0.026611 | 0.049095 | 0.015868 | 0.025112 | 0.020521 | 0.038483 | 0.016383 | 0.027087 | 0.021409 | 0.042915 |
Golgi | 0.072208 | 0.120144* | 0.008896 | 0.032424 | 0.090228 | 0.105444 | 0.021597 | 0.034779 | 0.090051 | 0.110572* | 0.019746 | 0.033795 |
Lipid Particles | 0.008691 | 0.112541* | 0.034682 | 0.063654 | 0.128190* | 0.152496* | 0.049910 | 0.073819 | 0.127016* | 0.138559* | 0.047690 | 0.069574 |
Mitochondria | 0.004980 | 0.026934 | 0.000377 | 0.002892 | 0.027522 | 0.031963 | 0.002163 | 0.004293 | 0.027301 | 0.030208 | 0.001903 | 0.003708 |
Mitotic Spindle | 0.003070 | 0.002234 | 0.007568 | 0.008552 | 0.000188 | 0.000591 | 0.000359 | 0.006474 | 0.000216 | 0.001164 | 0.001410 | 0.007342 |
None | 0.000389 | 0.002607 | 0.000480 | 0.000704 | 0.002878 | 0.002023 | 0.004874 | 0.000929 | 0.002854 | 0.002227 | 0.004233 | 0.000835 |
Nuclear Periphery | 0.000623 | 0.001631 | 0.004845 | 0.000902 | 0.001433 | 0.001577 | 0.000154 | 0.000669 | 0.001425 | 0.001596 | 0.000838 | 0.000767 |
Nuclear Periphery Foci | 0.004530 | 0.006933 | 0.092656 | 0.020303 | 0.004705 | 0.004880 | 0.003666 | 0.015332 | 0.004703 | 0.005596 | 0.016637 | 0.017408 |
Nucleolus | 0.004376 | 0.000202 | 0.000117 | 0.000071 | 0.000218 | 0.000394 | 0.000018 | 0.000068 | 0.000259 | 0.000327 | 0.000033 | 0.000069 |
Nucleus | 0.076080 | 0.003610 | 0.011607 | 0.001494 | 0.008281 | 0.008498 | 0.000312 | 0.002885 | 0.008948 | 0.006793 | 0.001958 | 0.002304 |
Peroxisomes | 0.001673 | 0.016208 | 0.001151 | 0.002453 | 0.022197 | 0.020245 | 0.006137 | 0.004496 | 0.021995 | 0.018837 | 0.005411 | 0.003643 |
Vacuole | 0.060802 | 0.012329 | 0.034799 | 0.034121 | 0.007729 | 0.012449 | 0.002612 | 0.027217 | 0.008250 | 0.012407 | 0.007304 | 0.030101 |
Vacuole Periphery | 0.021088 | 0.007773 | 0.001027 | 0.003452 | 0.004289 | 0.006772 | 0.001046 | 0.003153 | 0.004454 | 0.007121 | 0.001043 | 0.003278 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.32 | 1.18 | 0.70 | -7.17 | -4.12 | -2.04 | -6.17 | -8.66 | -7.43 | -3.90 | -1.75 | -6.05 | -11.06 | -10.18 | -5.24 |
Bud Neck | 1.29 | 1.01 | -0.71 | -3.89 | -3.46 | -1.85 | 3.41 | -5.59 | -4.76 | -6.93 | -1.82 | 2.61 | -7.09 | -6.09 | -8.84 |
Bud Site | 1.56 | 1.01 | 0.34 | -9.06 | -3.45 | -0.30 | -3.04 | -11.77 | -11.65 | -10.49 | -1.02 | -4.26 | -15.19 | -14.89 | -12.54 |
Cell Periphery | 1.21 | 1.46 | 0.23 | -5.43 | -6.94 | -0.81 | -6.08 | -5.15 | -3.93 | -1.46 | -0.54 | -5.37 | -7.07 | -5.96 | -2.86 |
Cytoplasm | 0.19 | -0.18 | 1.28 | 10.61 | 7.79 | 2.88 | 29.21 | 18.36 | 15.28 | -9.83 | 5.46 | 24.70 | 21.33 | 18.88 | -4.06 |
Cytoplasmic Foci | -1.07 | 0.22 | 0.40 | 9.75 | 1.13 | -5.92 | 19.43 | 9.72 | 15.06 | -9.29 | -7.25 | 17.13 | 9.08 | 17.52 | -9.36 |
Eisosomes | -2.50 | 1.04 | -0.48 | 3.76 | -3.78 | 0.05 | 6.06 | 5.27 | 4.45 | -0.87 | 1.13 | 6.75 | 6.41 | 5.70 | -0.76 |
Endoplasmic Reticulum | -0.41 | -1.66 | -2.10 | -10.32 | -2.18 | 5.95 | -35.86* | -12.33 | -17.88 | 18.40 | 4.83 | -32.23 | -13.96 | -20.57 | 18.12 |
Endosome | 1.54 | 1.71 | 0.72 | -6.09 | -7.02 | -6.67 | -3.84 | -12.24 | -6.56 | -9.13 | -8.60 | -4.33 | -16.36 | -9.20 | -12.71 |
Golgi | -1.06 | 1.27 | 0.78 | 15.78 | -8.49 | -3.41 | 21.38 | 15.37 | 17.93 | -5.24 | -5.05 | 22.28 | 16.80 | 23.91 | -7.09 |
Lipid Particles | -17.17* | -6.47 | -11.43 | 7.43 | -6.18 | -3.98 | 16.52 | 10.12 | 13.38 | -5.47 | -2.18 | 17.46 | 12.03 | 15.52 | -6.43 |
Mitochondria | -6.66 | 2.12 | 0.89 | 9.08 | -3.45 | -1.64 | 14.09 | 12.35 | 13.17 | -3.29 | -1.22 | 14.28 | 12.93 | 16.06 | -3.55 |
Mitotic Spindle | 0.39 | -1.48 | -2.67 | -3.65 | -0.35 | -2.03 | -1.63 | -4.84 | -4.48 | -4.71 | -2.73 | -2.88 | -7.22 | -5.97 | -5.61 |
None | -2.48 | -0.19 | -0.75 | 2.14 | -0.58 | 1.79 | -2.17 | 4.12 | 2.50 | 4.44 | 1.24 | -1.70 | 4.84 | 3.34 | 4.55 |
Nuclear Periphery | -1.77 | -5.36 | -0.79 | 1.49 | 5.42 | -0.40 | 5.03 | 2.69 | 3.17 | -5.00 | -0.51 | 2.17 | 2.35 | 3.29 | 0.22 |
Nuclear Periphery Foci | -0.63 | -11.74 | -4.78 | -5.61 | 9.68 | -0.37 | 1.53 | -6.60 | -6.50 | -7.18 | -1.44 | -8.93 | -9.36 | -8.71 | -0.62 |
Nucleolus | 0.98 | 1.00 | 1.01 | 2.03 | 1.16 | -1.73 | 4.29 | 3.11 | 3.38 | -4.06 | -0.83 | 3.60 | 2.94 | 3.84 | -2.36 |
Nucleus | 1.70 | 1.52 | 1.74 | 4.00 | 6.73 | -0.23 | 13.48 | 7.17 | 5.78 | -7.32 | 2.35 | 8.78 | 7.98 | 6.82 | -1.61 |
Peroxisomes | -9.65 | 0.68 | -1.19 | 9.75 | -3.27 | 1.13 | 11.49 | 12.87 | 13.09 | 2.21 | 2.04 | 12.21 | 13.99 | 16.56 | 3.11 |
Vacuole | 2.22 | 1.16 | 1.16 | -7.50 | -0.06 | -4.30 | 7.53 | -9.30 | -6.93 | -11.84 | -4.21 | 0.80 | -12.51 | -10.19 | -13.01 |
Vacuole Periphery | 1.03 | 1.55 | 1.36 | 4.38 | -3.62 | -3.86 | 9.76 | 2.10 | 4.91 | -4.31 | -4.80 | 9.91 | 2.43 | 6.48 | -5.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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