Standard name | |
---|---|
Human Ortholog | |
Description | Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 80 | 86 | 18 | 15 | 28 | 35 | 16 | 31 | – | 29 | 14 | 40 | 73 | 51 | 53 | 2 | – | 2 | 21 | 35 | 7 | 11 | 7 | 19 |
Bud | 5 | 4 | 8 | 4 | 10 | 7 | 18 | 22 | – | 6 | 12 | 17 | 7 | 2 | 3 | 2 | – | 0 | 1 | 5 | 0 | 0 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 3 | 0 | 4 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 0 | 1 | 0 | 0 | 3 | 3 | – | 2 | 4 | 0 | 1 | 1 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 39 | 6 | 21 | 16 | 15 | 19 | 40 | – | 31 | 43 | 46 | 13 | 8 | 21 | 3 | – | 0 | 1 | 7 | 0 | 0 | 1 | 4 |
Endoplasmic Reticulum | 14 | 32 | 3 | 3 | 5 | 14 | 19 | 19 | – | 15 | 13 | 25 | 27 | 15 | 5 | 0 | – | 0 | 1 | 3 | 0 | 0 | 2 | 2 |
Endosome | 28 | 12 | 23 | 32 | 41 | 26 | 35 | 23 | – | 43 | 13 | 22 | 12 | 41 | 32 | 1 | – | 0 | 90 | 63 | 24 | 55 | 63 | 72 |
Golgi | 244 | 194 | 34 | 66 | 67 | 39 | 32 | 20 | – | 35 | 15 | 30 | 137 | 253 | 374 | 13 | – | 13 | 227 | 191 | 64 | 108 | 148 | 222 |
Mitochondria | 36 | 21 | 51 | 86 | 82 | 104 | 248 | 166 | – | 131 | 127 | 144 | 9 | 16 | 28 | 4 | – | 2 | 2 | 6 | 1 | 2 | 6 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | – | 2 | 3 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 16 | 10 | 2 | 3 | 7 | 8 | 10 | 11 | – | 8 | 6 | 13 | 2 | 8 | 11 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 3 | – | 1 | 0 | 2 | 0 | 2 | 0 | 0 | – | 0 | 16 | 12 | 2 | 9 | 6 | 8 |
SpindlePole | 7 | 1 | 1 | 4 | 6 | 2 | 9 | 2 | – | 9 | 0 | 3 | 0 | 6 | 14 | 0 | – | 0 | 6 | 2 | 1 | 1 | 3 | 2 |
Vac/Vac Membrane | 32 | 24 | 2 | 6 | 8 | 5 | 15 | 16 | – | 18 | 12 | 9 | 18 | 51 | 52 | 0 | – | 3 | 4 | 11 | 0 | 1 | 2 | 6 |
Unique Cell Count | 326 | 304 | 97 | 172 | 188 | 165 | 336 | 277 | 251 | 207 | 260 | 204 | 337 | 470 | 17 | 16 | 378 | 343 | 108 | 192 | 251 | 349 | ||
Labelled Cell Count | 477 | 427 | 148 | 244 | 272 | 255 | 432 | 358 | 333 | 262 | 356 | 300 | 455 | 599 | 25 | 20 | 378 | 343 | 108 | 192 | 251 | 349 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 16.2 | 10.2 | 10.1 | 9.5 | 8.9 | 8.0 | 8.6 | – | 8.5 | 8.1 | 8.6 | 14.8 | 15.5 | 15.5 | 20.2 | – | 21.7 | 16.5 | 16.1 | 16.6 |
Std Deviation (1e-4) | 2.3 | 2.6 | 2.1 | 2.0 | 1.8 | 1.6 | 1.6 | 1.8 | – | 1.7 | 1.7 | 1.7 | 2.3 | 2.6 | 2.7 | 4.7 | – | 5.2 | 2.2 | 3.0 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.11 | -0.2 | -0.36 | -0.24 | – | -0.26 | -0.34 | -0.24 | 0.53 | 0.6 | 0.61 | 0.98 | – | 1.09 | 0.69 | 0.66 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -0.8 | 0.5 | -4.4 | -1.9 | – | -1.7 | -3.1 | -0.7 | 3.0 | -0.8 | -2.0 | 0 | – | 0 |
Bud | 0 | -1.0 | -1.3 | -1.1 | -0.1 | – | 0 | -0.8 | -0.6 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | 1.6 | 0.7 | 0.8 | -0.2 | 2.1 | – | 1.7 | 3.2 | 2.7 | 0.1 | 0 | 0 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 1.7 | 0 | 1.4 | – | 1.1 | 1.2 | 2.0 | 2.7 | 0 | 0 | 0 | – | 0 |
Endosome | -1.0 | -0.4 | -1.6 | -3.4 | -4.0 | – | -1.4 | -4.4 | -3.9 | -4.5 | -2.8 | -5.1 | 0 | – | 0 |
Golgi | 0.5 | 0.1 | -2.0 | -6.2 | -6.7 | – | -4.4 | -6.1 | -5.2 | 5.3 | 7.3 | 8.9 | 3.2 | – | 3.5 |
Mitochondria | -0.4 | -1.4 | 1.7 | 4.0 | 1.3 | – | -0.1 | 1.4 | 0.5 | -9.8 | -11.5 | -12.1 | -2.2 | – | -3.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 1.8 | 0 | 0 | 2.2 | 3.5 | 2.7 | 0 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.3672 | 13.4394 | 12.3456 | 12.4234 | 12.6478 | 13.079 | 13.5114 | 15.2595 | 14.1098 | 13.1882 | 13.7005 | 15.1719 | 12.1538 | 14.7669 | 12.7123 | 13.112 | 11.3759 | 14.1345 |
Actin | 0.0188 | 0.0112 | 0.0396 | 0.0482 | 0.0049 | 0.0182 | 0.0747 | 0.0378 | 0.0338 | 0.0505 | 0.0009 | 0.0258 | 0.041 | 0.0138 | 0.0107 | 0.0033 | 0.0063 | 0.0109 |
Bud | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0.0002 | 0.0005 | 0.0009 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0004 | 0.0019 | 0.0002 | 0.0002 | 0.0003 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 |
Bud Site | 0.0004 | 0.0004 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0014 | 0.0048 | 0.001 | 0.0004 | 0.0001 | 0.0001 | 0.0011 | 0.0013 | 0.0029 | 0.0001 | 0.0011 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.006 | 0.0034 | 0.0062 | 0.0026 | 0.0019 | 0.0036 | 0.0239 | 0.0032 | 0.0127 | 0.0292 | 0.0017 | 0.0121 | 0.0037 | 0.0085 | 0.0022 | 0.0042 | 0.0027 | 0.0023 |
Cytoplasmic Foci | 0.1329 | 0.1786 | 0.1485 | 0.099 | 0.1414 | 0.0812 | 0.1199 | 0.1613 | 0.1088 | 0.1538 | 0.1455 | 0.1072 | 0.1145 | 0.1529 | 0.1031 | 0.091 | 0.1151 | 0.0738 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0003 | 0.0005 | 0.0015 | 0.0003 | 0.0006 | 0.003 | 0.0003 | 0.001 | 0.0013 | 0.0004 | 0.0014 | 0.0011 | 0.0006 | 0.0005 | 0.0004 | 0.0016 | 0.0004 |
Endosome | 0.1957 | 0.1751 | 0.1603 | 0.1414 | 0.1429 | 0.1363 | 0.1403 | 0.1197 | 0.1516 | 0.212 | 0.1953 | 0.0959 | 0.1888 | 0.1851 | 0.1734 | 0.253 | 0.2204 | 0.1161 |
Golgi | 0.6033 | 0.5858 | 0.6024 | 0.6469 | 0.6937 | 0.742 | 0.5272 | 0.6231 | 0.6596 | 0.5182 | 0.6224 | 0.6945 | 0.5749 | 0.617 | 0.6668 | 0.6068 | 0.6127 | 0.7598 |
Lipid Particles | 0.0132 | 0.0202 | 0.0191 | 0.0426 | 0.0091 | 0.0079 | 0.0552 | 0.0099 | 0.0079 | 0.0189 | 0.0186 | 0.0274 | 0.0296 | 0.0045 | 0.014 | 0.0239 | 0.0215 | 0.025 |
Mitochondria | 0.0104 | 0.0036 | 0.0031 | 0.0031 | 0.0013 | 0.0026 | 0.0052 | 0.0034 | 0.0019 | 0.0021 | 0.0009 | 0.0029 | 0.0153 | 0.0023 | 0.0034 | 0.0036 | 0.0035 | 0.002 |
None | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0019 | 0.0003 | 0.0011 | 0.0003 | 0.0001 | 0.0002 | 0.0018 | 0 | 0.0008 | 0 | 0.0006 | 0 |
Nuclear Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0056 | 0.0005 | 0.0019 | 0.0002 | 0.0002 | 0.0046 | 0.0017 | 0.0002 | 0.0004 | 0.0001 | 0.0011 | 0.0001 |
Nucleolus | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0018 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0023 | 0.0001 | 0 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 |
Peroxisomes | 0.0101 | 0.0176 | 0.0166 | 0.0107 | 0.0016 | 0.0046 | 0.0283 | 0.0327 | 0.011 | 0.0103 | 0.009 | 0.0247 | 0.0147 | 0.0105 | 0.0154 | 0.0098 | 0.0077 | 0.007 |
Punctate Nuclear | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0007 | 0.0001 | 0.0079 | 0.0007 | 0.0013 | 0.0003 | 0.0034 | 0.0012 | 0.0037 | 0.0002 | 0.0017 | 0.0001 | 0.0008 | 0.0001 |
Vacuole | 0.0017 | 0.0011 | 0.0005 | 0.0006 | 0.0004 | 0.0008 | 0.0013 | 0.0004 | 0.0018 | 0.0009 | 0.0005 | 0.0004 | 0.0019 | 0.0014 | 0.0008 | 0.0014 | 0.0019 | 0.0008 |
Vacuole Periphery | 0.002 | 0.0011 | 0.0007 | 0.0011 | 0.0006 | 0.001 | 0.0012 | 0.0005 | 0.0014 | 0.0008 | 0.0007 | 0.001 | 0.0017 | 0.0007 | 0.001 | 0.0018 | 0.0019 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 53.9709 | 50.0336 | 72.9282 | 84.3154 | 62.2005 | 45.9497 | 74.0766 | 64.0202 | 76.384 | 72.903 |
Translational Efficiency | 2.5082 | 2.7176 | 1.9278 | 1.5111 | 1.9821 | 2.7959 | 2.0209 | 2.3148 | 1.8128 | 1.7899 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
206 | 1333 | 2047 | 108 | 1482 | 1953 | 2638 | 896 | 1688 | 3286 | 4685 | 1004 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 999.74 | 1296.28 | 1722.13 | 1840.58 | 1014.96 | 1319.11 | 1767.74 | 1592.33 | 1013.10 | 1309.85 | 1747.81 | 1619.03 |
Standard Deviation | 190.96 | 234.37 | 310.95 | 422.90 | 222.15 | 252.72 | 400.92 | 343.33 | 218.64 | 245.70 | 365.05 | 361.04 |
Intensity Change Log 2 | — | 0.374753 | 0.784569 | 0.880536 | — | 0.378142 | 0.800483 | 0.649716 | — | 0.376461 | 0.792608 | 0.768867 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.013287 | 0.019953 | 0.007991 | 0.057375 | 0.008872 | 0.012124 | 0.014431 | 0.024101 | 0.009410 | 0.015300 | 0.011617 | 0.027681 |
Bud Neck | 0.005329 | 0.000675 | 0.001960 | 0.008226 | 0.001557 | 0.000532 | 0.001182 | 0.002753 | 0.002017 | 0.000590 | 0.001522 | 0.003341 |
Bud Site | 0.010410 | 0.003345 | 0.004920 | 0.049886 | 0.006008 | 0.004432 | 0.008259 | 0.032286 | 0.006546 | 0.003991 | 0.006800 | 0.034180 |
Cell Periphery | 0.000228 | 0.000106 | 0.000124 | 0.000370 | 0.000244 | 0.000056 | 0.000153 | 0.000266 | 0.000243 | 0.000076 | 0.000140 | 0.000277 |
Cytoplasm | 0.004150 | 0.002307 | 0.001974 | 0.005384 | 0.005474 | 0.004486 | 0.004761 | 0.017335 | 0.005313 | 0.003602 | 0.003543 | 0.016050 |
Cytoplasmic Foci | 0.288942* | 0.320449* | 0.201963* | 0.222758* | 0.254649* | 0.333567* | 0.166182 | 0.243448* | 0.258834* | 0.328245* | 0.181816* | 0.241223* |
Eisosomes | 0.000130 | 0.000086 | 0.000047 | 0.000117 | 0.000094 | 0.000049 | 0.000075 | 0.000078 | 0.000098 | 0.000064 | 0.000063 | 0.000082 |
Endoplasmic Reticulum | 0.001966 | 0.000901 | 0.001174 | 0.003204 | 0.001355 | 0.000438 | 0.000531 | 0.002444 | 0.001429 | 0.000626 | 0.000812 | 0.002525 |
Endosome | 0.150923 | 0.147647 | 0.163360 | 0.182078 | 0.182716 | 0.162378 | 0.212420* | 0.172104 | 0.178836 | 0.156403 | 0.190984 | 0.173177 |
Golgi | 0.402279* | 0.436922* | 0.451983* | 0.251934* | 0.420724* | 0.430401* | 0.477962* | 0.357009* | 0.418473* | 0.433046* | 0.466612* | 0.345706* |
Lipid Particles | 0.023964 | 0.042486 | 0.078493 | 0.062533 | 0.054185 | 0.015735 | 0.047999 | 0.046745 | 0.050497 | 0.026587 | 0.061323 | 0.048443 |
Mitochondria | 0.041430 | 0.004638 | 0.003633 | 0.000454 | 0.025061 | 0.006419 | 0.009719 | 0.002407 | 0.027058 | 0.005696 | 0.007060 | 0.002197 |
Mitotic Spindle | 0.016481 | 0.004098 | 0.026820 | 0.054966 | 0.004735 | 0.011628 | 0.022993 | 0.053517 | 0.006168 | 0.008574 | 0.024665 | 0.053673 |
None | 0.000103 | 0.000044 | 0.000040 | 0.000031 | 0.000256 | 0.000023 | 0.000063 | 0.000051 | 0.000237 | 0.000032 | 0.000053 | 0.000049 |
Nuclear Periphery | 0.000085 | 0.000067 | 0.001249 | 0.001011 | 0.000092 | 0.000042 | 0.000350 | 0.000256 | 0.000092 | 0.000052 | 0.000743 | 0.000338 |
Nuclear Periphery Foci | 0.000941 | 0.001141 | 0.006733 | 0.021439 | 0.004575 | 0.000521 | 0.001648 | 0.006536 | 0.004132 | 0.000772 | 0.003870 | 0.008139 |
Nucleolus | 0.000184 | 0.000031 | 0.000061 | 0.000031 | 0.000869 | 0.000031 | 0.000026 | 0.000020 | 0.000786 | 0.000031 | 0.000042 | 0.000021 |
Nucleus | 0.003824 | 0.000052 | 0.000059 | 0.000117 | 0.000654 | 0.000044 | 0.000067 | 0.000186 | 0.001041 | 0.000047 | 0.000063 | 0.000178 |
Peroxisomes | 0.021895 | 0.007479 | 0.002683 | 0.009251 | 0.015107 | 0.009270 | 0.004757 | 0.003485 | 0.015935 | 0.008544 | 0.003851 | 0.004106 |
Vacuole | 0.008660 | 0.005411 | 0.034185 | 0.064669 | 0.008645 | 0.005649 | 0.019357 | 0.030115 | 0.008646 | 0.005552 | 0.025836 | 0.033831 |
Vacuole Periphery | 0.004788 | 0.002163 | 0.010549 | 0.004167 | 0.004128 | 0.002175 | 0.007064 | 0.004857 | 0.004209 | 0.002170 | 0.008587 | 0.004783 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.50 | 1.30 | -2.69 | -2.32 | -3.11 | -1.88 | -3.42 | -5.34 | -4.06 | -3.31 | -3.72 | -1.67 | -5.89 | -3.95 | -5.26 |
Bud Neck | 1.50 | 1.06 | -1.03 | -6.72 | -5.57 | 4.73 | 1.41 | -4.03 | -9.08 | -5.98 | 3.40 | 1.06 | -3.03 | -10.82 | -6.93 |
Bud Site | 3.32 | 2.43 | -4.24 | -5.03 | -4.85 | 1.80 | -2.58 | -10.32 | -11.14 | -9.36 | 3.33 | -0.72 | -11.11 | -12.39 | -11.09 |
Cell Periphery | 3.16 | 2.81 | -2.57 | -5.83 | -5.63 | 3.72 | 1.70 | -0.69 | -11.48 | -5.45 | 3.68 | 2.20 | -1.02 | -11.37 | -7.65 |
Cytoplasm | 1.52 | 1.57 | -0.86 | -2.50 | -2.56 | 0.78 | 0.39 | -6.09 | -6.61 | -6.59 | 1.78 | 1.68 | -6.15 | -7.29 | -7.30 |
Cytoplasmic Foci | -2.31 | 6.50 | 2.87 | 4.99 | -1.13 | -13.49 | 17.90 | 1.79 | 12.46 | -11.30 | -13.72 | 16.97 | 2.75 | 13.40 | -9.31 |
Eisosomes | 2.58 | 5.01 | 0.60 | -1.47 | -3.52 | 6.50 | 2.55 | 1.91 | -5.30 | -0.49 | 5.26 | 5.53 | 2.06 | -3.31 | -3.61 |
Endoplasmic Reticulum | 2.40 | 1.74 | -0.91 | -1.74 | -1.53 | 5.43 | 4.86 | -3.19 | -6.50 | -6.20 | 5.10 | 3.77 | -3.23 | -6.13 | -5.48 |
Endosome | 0.30 | -1.19 | -1.85 | -2.51 | -1.31 | 3.92 | -5.50 | 2.03 | -1.54 | 7.59 | 4.99 | -2.60 | 1.29 | -3.23 | 4.01 |
Golgi | -2.10 | -2.83 | 5.36 | 7.50 | 7.96 | -1.21 | -6.90 | 6.53 | 7.71 | 11.99 | -2.14 | -6.61 | 7.81 | 10.00 | 13.19 |
Lipid Particles | -2.85 | -8.23 | -2.89 | -1.60 | 1.25 | 9.84 | 1.36 | 1.40 | -9.04 | 0.20 | 6.55 | -2.79 | 0.33 | -6.36 | 3.27 |
Mitochondria | 5.02 | 5.17 | 5.62 | 5.76 | 4.78 | 7.95 | 6.46 | 9.94 | 3.65 | 6.31 | 9.74 | 9.10 | 11.33 | 4.16 | 5.72 |
Mitotic Spindle | 2.95 | -2.16 | -2.96 | -4.16 | -2.23 | -4.27 | -9.51 | -10.27 | -8.52 | -5.90 | -1.96 | -11.52 | -10.63 | -10.02 | -6.17 |
None | 2.51 | 2.99 | 3.39 | 0.99 | 1.42 | 4.29 | 3.49 | 3.69 | -4.92 | 0.75 | 4.30 | 3.84 | 3.87 | -2.80 | 0.16 |
Nuclear Periphery | 1.39 | -9.11 | -2.27 | -2.32 | 0.56 | 5.29 | -9.06 | -5.98 | -8.06 | 2.27 | 4.62 | -11.18 | -4.97 | -5.80 | 5.18 |
Nuclear Periphery Foci | -0.05 | -6.18 | -2.60 | -2.60 | -1.85 | 3.70 | 2.61 | -1.39 | -5.04 | -4.09 | 3.49 | 0.07 | -2.55 | -5.42 | -3.03 |
Nucleolus | 1.14 | 0.89 | 1.12 | -0.20 | 2.36 | 3.18 | 3.35 | 3.38 | 0.86 | 1.09 | 3.34 | 3.35 | 3.47 | 0.94 | 3.16 |
Nucleus | 1.11 | 1.10 | 1.09 | -1.53 | -1.21 | 2.46 | 2.39 | 1.63 | -2.81 | -2.42 | 2.11 | 2.08 | 1.72 | -2.98 | -2.66 |
Peroxisomes | 3.45 | 4.68 | 2.92 | -1.00 | -4.27 | 3.67 | 7.36 | 8.49 | 6.82 | 2.31 | 5.20 | 9.25 | 9.01 | 6.52 | -0.42 |
Vacuole | 1.85 | -11.05 | -5.50 | -5.89 | -3.06 | 3.62 | -9.70 | -10.56 | -12.11 | -5.29 | 4.41 | -16.38 | -11.79 | -13.44 | -4.01 |
Vacuole Periphery | 2.44 | -4.90 | 0.53 | -2.72 | 7.44 | 6.14 | -7.55 | -1.47 | -5.75 | 4.25 | 6.99 | -11.51 | -1.23 | -6.35 | 7.92 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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