Standard name
Human Ortholog
Description Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.25 0.28 0.19 0.09 0.15 0.21 0.05 0.11 – 0.12 0.07 0.15 0.36 0.15 0.11 0.12 – 0.12 0.06 0.1 0.07 0.06 0 0.06
Bud 0 0 0.08 0 0.05 0 0.05 0.08 – 0 0.06 0.07 0 0 0 0.12 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0.13 0.06 0.12 0.09 0.09 0.06 0.14 – 0.12 0.21 0.18 0.06 0 0 0.18 – 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.11 0 0 0 0.08 0.06 0.07 – 0.06 0.06 0.1 0.13 0 0 0 – 0 0 0 0 0 0 0
Endosome 0.09 0 0.24 0.19 0.22 0.16 0.1 0.08 – 0.17 0.06 0.08 0.06 0.12 0.07 0.06 – 0 0.24 0.18 0.23 0.29 0.25 0.21
Golgi 0.75 0.64 0.35 0.38 0.36 0.24 0.1 0.07 – 0.14 0.07 0.12 0.67 0.75 0.8 0.76 – 0.81 0.6 0.56 0.6 0.56 0.59 0.64
Mitochondria 0.11 0.07 0.53 0.5 0.44 0.63 0.74 0.6 – 0.52 0.61 0.55 0 0.05 0.06 0.24 – 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.05 0 0 – 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.08 0 0 0 0 0 0.06 – 0.07 0.06 0 0.09 0.15 0.11 0 – 0.19 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 80 86 18 15 28 35 16 31 – 29 14 40 73 51 53 2 – 2 21 35 7 11 7 19
Bud 5 4 8 4 10 7 18 22 – 6 12 17 7 2 3 2 – 0 1 5 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 – 3 0 4 0 0 0 0 – 0 – – – – – –
Cell Periphery 1 4 0 1 0 0 3 3 – 2 4 0 1 1 3 0 – 0 0 0 0 0 0 0
Cytoplasm 14 39 6 21 16 15 19 40 – 31 43 46 13 8 21 3 – 0 1 7 0 0 1 4
Endoplasmic Reticulum 14 32 3 3 5 14 19 19 – 15 13 25 27 15 5 0 – 0 1 3 0 0 2 2
Endosome 28 12 23 32 41 26 35 23 – 43 13 22 12 41 32 1 – 0 90 63 24 55 63 72
Golgi 244 194 34 66 67 39 32 20 – 35 15 30 137 253 374 13 – 13 227 191 64 108 148 222
Mitochondria 36 21 51 86 82 104 248 166 – 131 127 144 9 16 28 4 – 2 2 6 1 2 6 5
Nucleus 0 0 0 0 0 0 0 1 – 0 0 1 1 1 1 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 1 0 – 2 3 0 0 0 2 0 – 0 0 0 0 0 0 0
Nucleolus 16 10 2 3 7 8 10 11 – 8 6 13 2 8 11 0 – 0 0 0 1 0 0 0
Peroxisomes 0 0 0 2 1 0 5 3 – 1 0 2 0 2 0 0 – 0 16 12 2 9 6 8
SpindlePole 7 1 1 4 6 2 9 2 – 9 0 3 0 6 14 0 – 0 6 2 1 1 3 2
Vac/Vac Membrane 32 24 2 6 8 5 15 16 – 18 12 9 18 51 52 0 – 3 4 11 0 1 2 6
Unique Cell Count 326 304 97 172 188 165 336 277 251 207 260 204 337 470 17 16 378 343 108 192 251 349
Labelled Cell Count 477 427 148 244 272 255 432 358 333 262 356 300 455 599 25 20 378 343 108 192 251 349


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 16.2 10.2 10.1 9.5 8.9 8.0 8.6 – 8.5 8.1 8.6 14.8 15.5 15.5 20.2 – 21.7 16.5 16.1 16.6
Std Deviation (1e-4) 2.3 2.6 2.1 2.0 1.8 1.6 1.6 1.8 – 1.7 1.7 1.7 2.3 2.6 2.7 4.7 – 5.2 2.2 3.0 2.6
Intensity Change (Log2) – – – -0.01 -0.11 -0.2 -0.36 -0.24 – -0.26 -0.34 -0.24 0.53 0.6 0.61 0.98 – 1.09 0.69 0.66 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.4 -0.8 0.5 -4.4 -1.9 – -1.7 -3.1 -0.7 3.0 -0.8 -2.0 0 – 0
Bud 0 -1.0 -1.3 -1.1 -0.1 – 0 -0.8 -0.6 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Cytoplasm 1.6 0.7 0.8 -0.2 2.1 – 1.7 3.2 2.7 0.1 0 0 0 – 0
Endoplasmic Reticulum 0 0 1.7 0 1.4 – 1.1 1.2 2.0 2.7 0 0 0 – 0
Endosome -1.0 -0.4 -1.6 -3.4 -4.0 – -1.4 -4.4 -3.9 -4.5 -2.8 -5.1 0 – 0
Golgi 0.5 0.1 -2.0 -6.2 -6.7 – -4.4 -6.1 -5.2 5.3 7.3 8.9 3.2 – 3.5
Mitochondria -0.4 -1.4 1.7 4.0 1.3 – -0.1 1.4 0.5 -9.8 -11.5 -12.1 -2.2 – -3.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0 0 – 1.8 0 0 2.2 3.5 2.7 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.3672 13.4394 12.3456 12.4234 12.6478 13.079 13.5114 15.2595 14.1098 13.1882 13.7005 15.1719 12.1538 14.7669 12.7123 13.112 11.3759 14.1345
Actin 0.0188 0.0112 0.0396 0.0482 0.0049 0.0182 0.0747 0.0378 0.0338 0.0505 0.0009 0.0258 0.041 0.0138 0.0107 0.0033 0.0063 0.0109
Bud 0.0002 0.0001 0.0003 0.0003 0.0001 0.0001 0.0003 0.0006 0.0002 0.0001 0 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001
Bud Neck 0.0004 0.0004 0.0009 0.0003 0.0002 0.0005 0.0009 0.0003 0.0004 0.0004 0.0001 0.0004 0.0006 0.0004 0.0019 0.0002 0.0002 0.0003
Bud Periphery 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0003 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0004 0 0.0001 0
Bud Site 0.0004 0.0004 0.0006 0.0004 0.0001 0.0001 0.0014 0.0048 0.001 0.0004 0.0001 0.0001 0.0011 0.0013 0.0029 0.0001 0.0011 0.0001
Cell Periphery 0.0001 0.0001 0 0.0001 0 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.006 0.0034 0.0062 0.0026 0.0019 0.0036 0.0239 0.0032 0.0127 0.0292 0.0017 0.0121 0.0037 0.0085 0.0022 0.0042 0.0027 0.0023
Cytoplasmic Foci 0.1329 0.1786 0.1485 0.099 0.1414 0.0812 0.1199 0.1613 0.1088 0.1538 0.1455 0.1072 0.1145 0.1529 0.1031 0.091 0.1151 0.0738
Eisosomes 0.0001 0 0 0.0001 0 0 0.0003 0.0001 0.0001 0 0 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0014 0.0003 0.0005 0.0015 0.0003 0.0006 0.003 0.0003 0.001 0.0013 0.0004 0.0014 0.0011 0.0006 0.0005 0.0004 0.0016 0.0004
Endosome 0.1957 0.1751 0.1603 0.1414 0.1429 0.1363 0.1403 0.1197 0.1516 0.212 0.1953 0.0959 0.1888 0.1851 0.1734 0.253 0.2204 0.1161
Golgi 0.6033 0.5858 0.6024 0.6469 0.6937 0.742 0.5272 0.6231 0.6596 0.5182 0.6224 0.6945 0.5749 0.617 0.6668 0.6068 0.6127 0.7598
Lipid Particles 0.0132 0.0202 0.0191 0.0426 0.0091 0.0079 0.0552 0.0099 0.0079 0.0189 0.0186 0.0274 0.0296 0.0045 0.014 0.0239 0.0215 0.025
Mitochondria 0.0104 0.0036 0.0031 0.0031 0.0013 0.0026 0.0052 0.0034 0.0019 0.0021 0.0009 0.0029 0.0153 0.0023 0.0034 0.0036 0.0035 0.002
None 0.0006 0 0.0001 0.0002 0.0004 0 0.0019 0.0003 0.0011 0.0003 0.0001 0.0002 0.0018 0 0.0008 0 0.0006 0
Nuclear Periphery 0.0021 0.0001 0.0001 0.0003 0.0001 0.0002 0.0056 0.0005 0.0019 0.0002 0.0002 0.0046 0.0017 0.0002 0.0004 0.0001 0.0011 0.0001
Nucleolus 0 0.0001 0 0 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0018 0 0.0001 0.0001 0.0001 0
Nucleus 0.0002 0.0001 0 0 0.0001 0 0.0008 0.0001 0.0023 0.0001 0 0.0001 0.001 0.0001 0.0002 0 0.0002 0
Peroxisomes 0.0101 0.0176 0.0166 0.0107 0.0016 0.0046 0.0283 0.0327 0.011 0.0103 0.009 0.0247 0.0147 0.0105 0.0154 0.0098 0.0077 0.007
Punctate Nuclear 0.0003 0.0006 0.0004 0.0003 0.0007 0.0001 0.0079 0.0007 0.0013 0.0003 0.0034 0.0012 0.0037 0.0002 0.0017 0.0001 0.0008 0.0001
Vacuole 0.0017 0.0011 0.0005 0.0006 0.0004 0.0008 0.0013 0.0004 0.0018 0.0009 0.0005 0.0004 0.0019 0.0014 0.0008 0.0014 0.0019 0.0008
Vacuole Periphery 0.002 0.0011 0.0007 0.0011 0.0006 0.001 0.0012 0.0005 0.0014 0.0008 0.0007 0.001 0.0017 0.0007 0.001 0.0018 0.0019 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.9709 50.0336 72.9282 84.3154 62.2005 45.9497 74.0766 64.0202 76.384 72.903
Translational Efficiency 2.5082 2.7176 1.9278 1.5111 1.9821 2.7959 2.0209 2.3148 1.8128 1.7899

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor
Localization
Cell Percentages Golgi (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arl1

Arl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available