Standard name
Human Ortholog
Description Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.65 0.44 0.32 0.36 0.41 0.3 0.31 0.33 0.27 0.32 0.24 0.54 0.61 0.51 0.18 0.23 0.24 0.46 0.49 0.36 0.39 0.3 0.18
Endoplasmic Reticulum 0 0 0 0.05 0 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.13 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.05 0.23 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0.07 0.09 0.23 0.16 0.16 0.18 0.22 0.14 0.2 0.16 0.17 0 0.11 0.1 0.07 0 0.06 0.18 0.15 0.18 0.35 0.3 0.39
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.45 0.42 0.62 0.59 0.58 0.51 0.58 0.55 0.6 0.57 0.42 0.58 0.56 0.56 0.62 0.79 0.78 0.79 0.12 0.16 0.21 0.13 0.16 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 2 1 3 2 0 0 0 0 0 1 1 1 2 2 0 0 1 0
Bud 0 0 0 0 0 1 2 0 0 0 2 2 0 0 0 0 0 0 10 10 5 4 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 0 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 2 3 0 2 3 1 0 0 0 2 4 0 0 0 0 0 0 0
Cytoplasm 164 331 89 53 71 87 105 113 83 61 72 56 124 133 134 74 87 76 145 276 85 43 46 40
Endoplasmic Reticulum 6 18 6 9 8 12 19 26 4 7 4 1 6 8 1 5 9 9 22 45 30 3 6 5
Endosome 0 1 0 1 0 0 3 1 1 0 0 6 2 1 0 3 3 1 5 10 2 1 6 10
Golgi 1 3 0 0 1 0 0 0 0 0 0 0 1 0 2 7 13 7 1 1 1 0 0 1
Mitochondria 1 0 0 0 0 0 7 8 9 11 51 21 0 2 0 7 1 0 2 2 4 0 3 1
Nucleus 18 36 18 37 32 34 63 79 35 46 36 40 9 24 26 27 15 18 55 86 43 39 45 88
Nuclear Periphery 0 0 0 0 0 0 0 1 2 3 2 2 0 0 0 1 1 0 17 20 8 1 3 4
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 4 1 1 5 6
Vac/Vac Membrane 117 215 124 96 116 108 203 199 150 131 94 136 128 121 162 318 293 251 36 90 50 14 24 48
Unique Cell Count 260 506 201 164 199 212 348 364 249 229 222 235 230 218 262 403 376 317 314 561 240 113 153 228
Labelled Cell Count 307 604 238 196 228 244 406 431 287 263 264 266 270 289 326 446 428 363 314 561 240 113 153 228


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.9 12.7 10.9 10.8 11.5 10.1 9.8 9.8 9.3 8.9 8.4 8.3 12.1 11.5 11.5 16.2 15.7 15.8 11.7 11.2 12.7
Std Deviation (1e-4) 1.5 1.8 1.7 1.6 1.9 1.9 1.7 1.6 2.0 1.8 1.8 1.6 2.3 1.9 1.8 4.2 4.7 4.5 2.4 2.2 2.3
Intensity Change (Log2) – – – -0.01 0.08 -0.12 -0.16 -0.15 -0.24 -0.29 -0.37 -0.39 0.15 0.08 0.08 0.57 0.53 0.53 0.11 0.04 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -1.8 -0.7 -3.3 -3.1 -2.4 -3.8 -2.5 -4.5 2.0 3.4 1.5 -6.8 -5.3 -4.8
Endoplasmic Reticulum 1.2 0.6 1.3 1.3 2.0 0 0 0 0 -0.2 0.4 0 0 -0.4 -0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.1 7.2 4.3 0 0 0 0 0 0
Nucleus 3.6 2.2 2.2 2.9 3.8 1.7 3.2 2.2 2.5 -2.2 0.7 0.4 -1.0 -2.4 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 -0.7 -2.2 -0.8 -1.6 -0.3 -0.9 -4.0 -0.8 -1.3 -1.3 0 4.5 4.2 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.0176 7.6376 7.6939 6.8928 6.9662 7.2927 6.6044 7.3462 7.1039 6.4267 6.3463 6.7859 6.8354 8.4222 8.0438 8.3522 8.8784 7.6245
Actin 0.0001 0.0002 0.0003 0.0001 0.0004 0.0001 0.019 0.0004 0.0009 0.0009 0.0128 0.0013 0.0213 0.0005 0.0127 0.0106 0.0002 0.0002
Bud 0 0.0001 0.0018 0.0001 0.0001 0.0001 0.0004 0.0004 0.0001 0.0001 0.0002 0.0001 0.0058 0.0001 0.0001 0.001 0.0001 0.0001
Bud Neck 0.0005 0.0005 0.0009 0.0011 0.0012 0.0016 0.0032 0.0007 0.0005 0.0011 0.0023 0.0029 0.0012 0.0005 0.0013 0.0008 0.0014 0.002
Bud Periphery 0 0 0.0002 0 0.0001 0 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0051 0 0.0001 0.001 0 0
Bud Site 0.0002 0.0006 0.0005 0.0001 0.0002 0 0.0028 0.0014 0.0003 0.0002 0.0006 0.0001 0.0039 0.0005 0.0005 0.0017 0.0002 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0003 0 0 0.0001 0 0
Cytoplasm 0.2045 0.2905 0.2297 0.2293 0.3425 0.1855 0.2018 0.2817 0.2302 0.3307 0.2775 0.2185 0.2893 0.3842 0.3249 0.3852 0.4073 0.3062
Cytoplasmic Foci 0.0014 0.0013 0.0019 0.0007 0.0023 0.0005 0.0085 0.001 0.001 0.0069 0.0064 0.0013 0.0141 0.0006 0.0008 0.002 0.0054 0.0013
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0135 0.0067 0.0075 0.0126 0.0256 0.0109 0.0182 0.0094 0.0124 0.0357 0.0171 0.0187 0.0218 0.012 0.0136 0.0086 0.0059 0.0118
Endosome 0.0069 0.0056 0.0047 0.0033 0.0115 0.0033 0.0208 0.003 0.0055 0.0163 0.027 0.0077 0.0288 0.0015 0.0019 0.0119 0.0049 0.007
Golgi 0.0001 0.0001 0.0001 0 0.0001 0 0.0033 0.0001 0.0002 0.0003 0.0049 0.0002 0.0077 0 0.0001 0.0016 0.0001 0.0002
Lipid Particles 0.0001 0.0001 0.0001 0 0.0001 0 0.005 0.0001 0.0002 0.0001 0.0195 0.0001 0.0093 0 0.0001 0.0008 0 0.001
Mitochondria 0.0004 0.0003 0.0005 0.0003 0.0007 0.0003 0.0035 0.0003 0.0006 0.0009 0.006 0.0008 0.0049 0.0003 0.0003 0.0055 0.0005 0.0003
None 0.0108 0.0014 0.0061 0.0007 0.0056 0.0005 0.0042 0.0014 0.0014 0.0027 0.0023 0.0014 0.0119 0.002 0.0006 0.0025 0.0013 0.0013
Nuclear Periphery 0.1087 0.0637 0.0715 0.0935 0.0878 0.0713 0.1077 0.0591 0.0767 0.1028 0.1092 0.0798 0.1187 0.0702 0.0807 0.0464 0.0242 0.0602
Nucleolus 0.0006 0.0007 0.0008 0.0005 0.0005 0.0005 0.0011 0.0006 0.0005 0.0005 0.0018 0.0006 0.001 0.0003 0.0005 0.0008 0.0003 0.0005
Nucleus 0.6407 0.6192 0.6647 0.649 0.507 0.7191 0.5744 0.6331 0.662 0.487 0.4849 0.6563 0.4304 0.5221 0.5533 0.5096 0.5388 0.5988
Peroxisomes 0 0 0.0001 0 0 0 0.0067 0 0 0.0002 0.0065 0.0001 0.0071 0 0.0001 0.0005 0.0001 0
Punctate Nuclear 0.0043 0.0048 0.0054 0.0042 0.0055 0.0027 0.0124 0.0042 0.0038 0.0093 0.0152 0.0056 0.0097 0.0025 0.0059 0.0043 0.0072 0.0058
Vacuole 0.0057 0.0036 0.0028 0.0033 0.0072 0.0028 0.0049 0.0025 0.0028 0.003 0.0041 0.0034 0.0056 0.0021 0.002 0.0032 0.0017 0.0026
Vacuole Periphery 0.0012 0.0007 0.0007 0.0009 0.0016 0.0008 0.0015 0.0005 0.0008 0.0011 0.0015 0.0011 0.0019 0.0004 0.0005 0.0018 0.0003 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.5376 16.4113 26.8754 31.4512 20.9298 34.1657 30.1829 34.3708 36.6889 28.3047
Translational Efficiency 1.2932 2.0927 1.238 1.236 1.4944 1.3467 1.494 1.2777 1.2309 1.2601

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
600 1072 214 415 1230 1426 1317 400 1830 2498 1531 815

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 687.61 917.75 1374.21 1377.89 965.26 1006.97 1190.98 1535.77 874.23 968.68 1216.59 1455.38
Standard Deviation 120.65 165.33 161.92 343.71 131.72 154.71 173.81 295.12 182.82 165.36 183.55 330.35
Intensity Change Log 2 — 0.416511 0.998940 1.002798 — 0.061031 0.303160 0.669973 — 0.219675 0.634092 0.817859

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000341 0.000815 0.003105 0.002737 0.000349 0.000517 0.000906 0.002375 0.000346 0.000645 0.001214 0.002559
Bud Neck 0.002601 0.004651 0.018800 0.039056 0.002111 0.004566 0.008399 0.041982 0.002271 0.004603 0.009853 0.040492
Bud Site 0.003100 0.007240 0.008557 0.021089 0.001937 0.006126 0.003536 0.018379 0.002319 0.006604 0.004238 0.019759
Cell Periphery 0.000369 0.000189 0.000786 0.001617 0.000159 0.000127 0.000324 0.002086 0.000228 0.000154 0.000388 0.001847
Cytoplasm 0.366188* 0.494235* 0.105289* 0.224075* 0.456199* 0.423557* 0.165991* 0.149125* 0.426687* 0.453888* 0.157506* 0.187290*
Cytoplasmic Foci 0.059474 0.051436 0.006481 0.007697 0.038395 0.042130 0.003036 0.006380 0.045306 0.046124 0.003518 0.007051
Eisosomes 0.000077 0.000031 0.000066 0.000054 0.000028 0.000030 0.000032 0.000049 0.000044 0.000030 0.000037 0.000052
Endoplasmic Reticulum 0.013963 0.043188 0.051912 0.100568* 0.070092 0.046375 0.031386 0.089939 0.051689 0.045007 0.034255 0.095351
Endosome 0.004292 0.007588 0.010195 0.013247 0.005459 0.008022 0.003461 0.019180 0.005076 0.007836 0.004402 0.016159
Golgi 0.000910 0.002026 0.000505 0.006763 0.001124 0.002767 0.000132 0.006352 0.001054 0.002449 0.000184 0.006561
Lipid Particles 0.010311 0.007058 0.004494 0.011474 0.007360 0.007856 0.001571 0.015882 0.008327 0.007513 0.001980 0.013638
Mitochondria 0.001810 0.002501 0.002583 0.008062 0.001744 0.006906 0.002321 0.005808 0.001766 0.005016 0.002358 0.006955
Mitotic Spindle 0.000332 0.006012 0.002068 0.043417 0.003693 0.018697 0.006253 0.072236 0.002591 0.013254 0.005668 0.057561
None 0.032750 0.009409 0.002070 0.005740 0.003962 0.003539 0.005842 0.004468 0.013400 0.006058 0.005315 0.005116
Nuclear Periphery 0.005376 0.018861 0.024591 0.044974 0.023791 0.030170 0.022072 0.095944 0.017754 0.025316 0.022424 0.069990
Nuclear Periphery Foci 0.015068 0.039948 0.018838 0.096775 0.034943 0.033842 0.009848 0.144626 0.028427 0.036462 0.011105 0.120260
Nucleolus 0.008160 0.003251 0.001664 0.001529 0.001277 0.002399 0.001389 0.001750 0.003534 0.002765 0.001428 0.001637
Nucleus 0.453821* 0.253500* 0.627947* 0.286983* 0.315762* 0.314435* 0.691697* 0.255177* 0.361027* 0.288285* 0.682787* 0.271373*
Peroxisomes 0.001818 0.001039 0.002267 0.000248 0.000428 0.001918 0.000465 0.000199 0.000884 0.001541 0.000717 0.000224
Vacuole 0.018258 0.044460 0.107246 0.082703 0.030459 0.043872 0.041062 0.066176 0.026459 0.044124 0.050313 0.074592
Vacuole Periphery 0.000983 0.002562 0.000537 0.001192 0.000727 0.002151 0.000276 0.001888 0.000811 0.002327 0.000312 0.001534

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.81 -11.52 -9.02 -6.93 1.20 -2.69 -8.93 -3.29 -3.07 -2.14 -4.64 -12.93 -6.63 -5.79 -3.55
Bud Neck -2.07 -9.23 -13.54 -12.96 -7.13 -5.88 -13.69 -14.34 -13.71 -11.98 -5.05 -15.53 -19.82 -18.89 -15.64
Bud Site -3.03 -5.39 -7.32 -5.79 -4.77 -4.45 -5.71 -8.17 -6.08 -6.96 -5.60 -6.29 -11.03 -8.38 -9.31
Cell Periphery 3.21 -6.03 -6.47 -7.45 -4.21 3.39 -8.14 -4.80 -4.87 -4.46 3.75 -6.52 -7.33 -7.62 -6.66
Cytoplasm -9.02 19.82 12.40 23.91 -7.59 3.56 36.32 31.68 29.12 1.69 -2.94 39.09 31.05 36.00 -3.00
Cytoplasmic Foci 1.86 12.39 12.31 13.96 -1.37 -1.17 15.96 13.79 15.43 -5.47 -0.03 20.38 18.41 20.76 -6.58
Eisosomes 7.48 -0.30 1.14 -5.70 1.49 -0.79 -5.52 -5.03 -4.77 -3.51 5.22 -0.68 -4.39 -7.29 -4.23
Endoplasmic Reticulum -10.05 -7.38 -8.68 -4.37 -3.72 5.35 9.73 1.06 -2.76 -5.48 2.25 6.31 -3.30 -5.06 -7.71
Endosome -4.89 -4.25 -7.03 -3.52 -1.14 -3.97 4.08 -7.87 -5.48 -9.42 -5.86 1.52 -10.26 -6.39 -10.96
Golgi -2.43 2.87 -1.15 -0.58 -1.37 -2.73 4.73 -1.08 -0.18 -1.64 -3.48 5.92 -1.55 -0.50 -2.21
Lipid Particles 2.43 3.71 -1.76 -3.99 -4.99 -0.83 9.05 -5.84 -5.52 -8.15 1.03 9.94 -6.07 -6.72 -10.37
Mitochondria -1.27 -2.40 -2.39 -2.02 -1.87 -4.53 -2.56 -4.53 1.19 -3.69 -4.68 -3.27 -4.03 -0.60 -3.17
Mitotic Spindle -4.96 -4.12 -6.20 -5.23 -5.63 -5.43 -2.53 -7.18 -5.54 -6.59 -6.23 -3.41 -9.24 -7.29 -8.10
None 5.34 7.31 6.41 2.94 -5.07 0.53 -1.02 -0.33 -0.85 0.60 4.65 5.76 5.52 1.57 -0.59
Nuclear Periphery -6.70 -3.91 -7.84 -3.81 -2.97 -2.13 0.82 -6.65 -5.73 -7.02 -3.75 -2.04 -8.45 -6.55 -7.47
Nuclear Periphery Foci -6.70 -1.08 -8.62 -4.76 -7.29 0.25 9.46 -8.46 -8.53 -11.58 -3.25 8.11 -11.23 -9.63 -14.56
Nucleolus 2.51 3.82 2.93 0.99 -1.12 -1.62 -1.04 -2.18 -0.22 -1.80 0.78 3.20 1.18 0.49 -1.95
Nucleus 13.78 -7.42 6.87 -4.80 12.91 0.11 -38.91 2.32 2.26 27.34 8.95 -35.42 4.92 -1.46 32.83
Peroxisomes 2.26 0.63 4.46 6.01 1.28 -4.24 0.11 4.07 4.83 1.15 -2.57 1.74 5.26 6.24 1.64
Vacuole -8.61 -11.19 -13.50 -9.55 1.51 -5.38 -8.74 -11.17 -9.00 -6.96 -8.87 -13.59 -16.99 -13.14 -7.73
Vacuole Periphery -2.65 1.16 0.51 3.76 -1.45 -4.31 5.66 -1.19 2.18 -2.62 -5.06 3.67 -0.61 4.26 -3.54
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates that are subsequently delivered to the proteasome for degradation; also involved in the regulated destruction of resident ER membrane proteins, such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing (RUP)
Localization
Cell Percentages ER (23%), cytoplasm (22%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Npl4

Npl4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npl4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
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