Standard name
Human Ortholog
Description GYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.1 0.12 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.96 0.97 0.95 0.93 0.93 0.89 – 0.82 0.86 0.99 1.0 0.99 0.92 0.93 0.93 0.96 0.96 0.93 0.89 0.83 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.1 0.08 0.23 – 0.3 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.05 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 1 – 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 5 1 5 1 3 4 13 – 6 8 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 11 8 17 12 10 11 4 – 5 11 0 1 1 22 25 28 0 0 0 0 0 0
Cytoplasm 62 223 313 225 450 416 666 638 402 – 263 302 95 152 173 352 241 219 75 217 297 46 116 171
Endoplasmic Reticulum 1 4 0 4 2 1 9 0 0 – 0 7 0 0 1 14 11 3 1 2 6 2 17 12
Endosome 0 0 0 1 0 1 0 5 2 – 1 0 0 0 0 5 2 0 0 0 1 0 0 2
Golgi 0 0 2 0 0 0 0 0 0 – 0 0 0 0 1 1 0 3 0 0 0 0 0 0
Mitochondria 1 0 0 1 9 36 72 53 102 – 97 51 4 0 1 3 0 2 0 0 2 0 0 0
Nucleus 0 1 2 0 0 2 5 5 1 – 3 0 1 1 1 4 7 5 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 – 0 3 0 0 0 2 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 3 3 10 6 15 15 10 – 4 4 0 1 0 25 14 5 0 4 4 2 1 2
Unique Cell Count 63 227 321 234 465 438 720 688 453 321 350 96 152 175 383 260 236 79 227 320 52 140 192
Labelled Cell Count 64 231 336 243 494 476 781 733 536 380 386 100 155 178 429 300 265 79 227 320 52 140 192


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 11.1 9.7 9.0 8.4 6.1 5.8 5.9 4.9 – 4.6 5.1 9.8 10.5 10.1 14.2 14.4 14.2 9.8 9.7 10.6
Std Deviation (1e-4) 1.7 1.7 1.1 1.1 1.3 1.1 0.8 1.2 1.0 – 1.0 1.0 1.3 1.6 1.3 2.2 1.7 2.0 1.1 1.2 1.4
Intensity Change (Log2) – – – -0.12 -0.21 -0.68 -0.74 -0.72 -0.98 – -1.07 -0.92 0.01 0.11 0.06 0.55 0.57 0.55 0.01 0.0 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1.2 – 0 0.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 -0.5 -2.2 -1.9 -2.5 – 0 -0.2 0 0 0 1.4 3.1 3.9
Cytoplasm -0.9 -0.6 -1.8 -3.2 -3.0 -4.5 – -6.5 -5.3 0.9 2.0 1.0 -3.2 -2.7 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.5 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.3 5.9 5.1 9.1 – 0 7.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.3 0 1.3 1.4 1.4 – 0 0 0 0 0 3.8 3.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1014 8.5793 7.7999 7.5465 6.6285 8.1697 5.8008 7.4409 7.3756 6.1702 6.6604 7.1111 5.9709 7.6356 6.816 6.8533 7.5664 6.9633
Actin 0.0277 0.0013 0.0123 0.0003 0.0274 0.0034 0.0084 0.001 0.0061 0.0138 0.0071 0.0013 0.027 0.0003 0.0172 0.0006 0.0003 0.0168
Bud 0.0031 0.001 0.0022 0.0008 0.0006 0.0008 0.0005 0.0005 0.0004 0.0009 0.0006 0.0003 0.0005 0.0001 0.0007 0.0004 0.0054 0.0002
Bud Neck 0.0025 0.0006 0.0004 0.0008 0.0006 0.0013 0.0007 0.0002 0.0007 0.0022 0.0007 0.0008 0.0009 0.0002 0.0015 0.0003 0.0046 0.0008
Bud Periphery 0.0016 0.0009 0.0012 0.0003 0.0011 0.0006 0.0004 0.0003 0.0002 0.0012 0.0005 0.0002 0.0005 0 0.0008 0.0002 0.0007 0.0002
Bud Site 0.0051 0.0299 0.0027 0.0004 0.0018 0.0002 0.004 0.0022 0.0015 0.0009 0.001 0.0002 0.004 0.0003 0.003 0.0003 0.0084 0.0001
Cell Periphery 0.001 0.0021 0.0007 0.0003 0.0005 0.0004 0.0004 0.0003 0.0001 0.0003 0.0001 0.0001 0.0004 0.0001 0.0005 0.0002 0.0001 0.0001
Cytoplasm 0.7747 0.8996 0.8829 0.942 0.778 0.8214 0.7796 0.9425 0.8874 0.7926 0.7601 0.8652 0.7832 0.9643 0.8927 0.8626 0.8785 0.8671
Cytoplasmic Foci 0.0215 0.0108 0.0131 0.0104 0.0258 0.019 0.0315 0.0099 0.0186 0.0121 0.0481 0.0146 0.0204 0.0061 0.0115 0.0094 0.0219 0.0136
Eisosomes 0.0003 0 0.0002 0 0.0002 0 0.0001 0 0 0.0001 0 0 0.0002 0 0.0004 0 0 0.0001
Endoplasmic Reticulum 0.0363 0.0126 0.0256 0.002 0.0343 0.0484 0.058 0.0138 0.0106 0.0237 0.0113 0.0337 0.0724 0.0091 0.0112 0.074 0.0238 0.0396
Endosome 0.0475 0.015 0.013 0.0232 0.0677 0.0633 0.0566 0.0182 0.0427 0.054 0.0504 0.0404 0.0418 0.0103 0.0242 0.0322 0.0456 0.0408
Golgi 0.0149 0.0025 0.0033 0.0007 0.0087 0.0199 0.0155 0.0026 0.0083 0.0179 0.0261 0.0143 0.0075 0.0007 0.0052 0.0014 0.0015 0.0064
Lipid Particles 0.007 0.0004 0.0039 0.0001 0.0094 0.0001 0.0045 0 0.0016 0.003 0.0079 0.001 0.0029 0 0.0053 0.0001 0 0.0011
Mitochondria 0.0196 0.0134 0.0021 0.0003 0.005 0.0113 0.0058 0.0025 0.0086 0.0442 0.0375 0.0153 0.0027 0.0002 0.0105 0.001 0.0004 0.0031
None 0.0086 0.0018 0.0178 0.0028 0.0029 0.001 0.0038 0.0008 0.0008 0.0006 0.0009 0.0045 0.0038 0.0047 0.0021 0.0008 0.0005 0.0011
Nuclear Periphery 0.0033 0.0007 0.0029 0.0006 0.0038 0.0021 0.0054 0.0006 0.0022 0.0016 0.0206 0.0029 0.0107 0.0007 0.0014 0.0033 0.0011 0.0044
Nucleolus 0.0004 0.0002 0.0002 0.0001 0.0002 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0 0.0004 0 0.0001 0
Nucleus 0.0016 0.001 0.0009 0.0011 0.0014 0.0012 0.0017 0.0007 0.0008 0.0011 0.0053 0.0008 0.0031 0.0006 0.001 0.0014 0.0014 0.0009
Peroxisomes 0.0102 0.0001 0.0085 0 0.0207 0.0003 0.007 0.0001 0.0023 0.0008 0.0139 0.0004 0.0083 0 0.005 0 0.0002 0.0003
Punctate Nuclear 0.0008 0.0003 0.0011 0.0001 0.001 0.0001 0.0009 0.0001 0.0002 0.0002 0.0013 0.0006 0.0006 0.0001 0.0003 0.0001 0.0003 0.0006
Vacuole 0.0101 0.0049 0.0041 0.0125 0.0073 0.0029 0.0114 0.0029 0.005 0.022 0.0036 0.0021 0.0073 0.0017 0.004 0.0091 0.0037 0.0017
Vacuole Periphery 0.0023 0.0006 0.0009 0.0016 0.0017 0.0023 0.0036 0.0007 0.0016 0.0067 0.0028 0.0011 0.0018 0.0003 0.0012 0.0025 0.0016 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.0515 23.5168 25.0234 31.9735 28.638 23.3159 29.3858 28.9175 26.3169 30.5055
Translational Efficiency 1.4241 1.889 1.6836 1.2437 1.3747 1.5654 1.201 1.1716 1.2174 1.1523

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description GYF domain protein; involved in COPII vesicle formation; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Smy2

Smy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Smy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available