Standard name
Human Ortholog
Description Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.35 0.44 0.45 0.53 0.67 0.49 0.44 0.4 0.35 0.45 0.36 0.42 0.68 0.62 0.69 0.1 0.15 0.13 0.22 0.31 0.17 0.49 0.44 0.42
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.55 0.3 0.09 0.1 0.06 0.27 0.46 0.43 0.61 0.51 0.66 0.47 0 0 0 0 0.07 0.12 0.12 0.1 0.06 0.05 0.07 0.06
Nucleus 0.23 0.23 0.43 0.37 0.24 0.28 0.26 0.3 0.29 0.25 0.21 0.21 0.28 0.28 0.25 0.83 0.78 0.71 0.25 0.26 0.39 0.17 0.15 0.16
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.11 0.08 0.1 0.1
Vac/Vac Membrane 0.12 0.2 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0 0.07 0.1 0.22 0.14 0.11 0.09 0.12 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 4
Bud 0 0 1 1 0 2 4 5 7 4 12 1 0 0 0 1 0 0 0 0 1 3 4 14
Bud Neck 0 0 3 5 7 5 2 9 2 4 0 6 7 1 4 0 0 0 0 0 1 0 4 8
Bud Site 0 0 0 0 0 1 5 2 7 6 1 3 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 1 2 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 14 27 35 93 151 145 155 143 105 158 101 109 208 190 233 13 20 16 17 31 22 85 145 170
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 3 1 4 4 5 2 0 0 0 1 2 5
Endosome 0 0 1 2 1 3 3 1 1 0 1 2 1 11 3 0 1 7 2 1 1 3 6 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 0 2
Mitochondria 22 18 7 18 13 79 162 153 184 180 184 121 1 2 0 4 9 14 9 9 7 8 22 26
Nucleus 9 14 33 64 53 83 92 108 87 89 58 55 84 86 84 105 106 85 20 26 50 29 49 65
Nuclear Periphery 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 2 0 0 0 1 1 1 1 1 4 0 0 0 0 0 4 5 2 2 4 3 8 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 5 8 7 8 9 16 3 7 2 9 8 5 3 0 6 3 5 9 14 13 32 39
Vac/Vac Membrane 5 12 1 2 7 10 12 8 6 12 10 14 6 17 19 5 10 12 18 13 15 15 40 34
Unique Cell Count 40 61 77 175 225 294 351 358 304 352 278 258 305 304 339 127 136 120 82 101 132 175 333 408
Labelled Cell Count 50 76 86 193 240 340 445 446 403 461 373 321 320 315 351 133 161 146 82 101 132 175 333 408


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.1 4.5 4.7 4.8 3.9 3.8 4.2 3.7 3.7 3.4 3.7 4.9 5.3 5.5 8.0 7.7 7.5 4.8 5.2 5.5
Std Deviation (1e-4) 0.8 0.9 0.9 1.6 1.8 1.4 1.5 1.6 1.9 1.2 1.3 1.3 0.9 1.2 1.8 1.8 2.2 1.6 1.3 1.4 1.8
Intensity Change (Log2) – – – 0.04 0.09 -0.21 -0.26 -0.11 -0.3 -0.28 -0.43 -0.31 0.12 0.23 0.28 0.82 0.77 0.72 0.09 0.19 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6528 -0.4047 -0.2032 0.2909 -0.4089 -0.2324 0.0279 -0.2383 -0.0334 -0.0098 0.0558 -0.1677 -0.8304 -0.6172 -0.6308 -0.0522 -0.7319 -0.475
Actin 0.0629 0.0007 0.0195 0.0226 0.0108 0.0072 0.0347 0.0002 0.0217 0.0193 0.0074 0.0006 0.0047 0.0002 0.0018 0.0001 0.0003 0.0002
Bud 0.0019 0.002 0.0123 0.0005 0.0013 0.0009 0.0003 0.0002 0.0041 0.0005 0.0005 0.0002 0.0009 0.0004 0.0004 0 0.0004 0.0001
Bud Neck 0.0062 0.0025 0.0019 0.0021 0.0013 0.0043 0.0018 0.0008 0.0087 0.025 0.0022 0.0036 0.0009 0.0006 0.0006 0.0004 0.0014 0.0028
Bud Periphery 0.003 0.0016 0.0049 0.0006 0.0067 0.0008 0.0004 0.0001 0.0012 0.0005 0.0007 0.0003 0.0007 0.0001 0.0003 0 0.0005 0
Bud Site 0.0148 0.0044 0.016 0.0131 0.0014 0.0009 0.0046 0.0061 0.0081 0.0011 0.003 0.0004 0.0011 0.0014 0.0006 0.0001 0.0005 0.0001
Cell Periphery 0.0008 0.0001 0.0006 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0 0 0 0 0
Cytoplasm 0.0508 0.0708 0.0414 0.0242 0.0702 0.0498 0.0675 0.1062 0.0777 0.0782 0.0935 0.0664 0.0616 0.1002 0.0587 0.023 0.067 0.0561
Cytoplasmic Foci 0.0553 0.0108 0.012 0.0142 0.0167 0.019 0.0427 0.0062 0.0122 0.0148 0.0617 0.0039 0.0067 0.0045 0.0043 0.0015 0.0015 0.0029
Eisosomes 0.0006 0.0002 0.0003 0.0002 0.0005 0.0002 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0091 0.0014 0.0026 0.002 0.0037 0.0014 0.0029 0.0023 0.0017 0.0016 0.0048 0.0015 0.0015 0.0017 0.0014 0.0003 0.0008 0.0009
Endosome 0.0588 0.0016 0.0169 0.0146 0.0134 0.0224 0.0155 0.0024 0.0066 0.0102 0.0772 0.0079 0.0026 0.0012 0.0034 0.0006 0.0015 0.0021
Golgi 0.0165 0.0002 0.0083 0.0129 0.0057 0.0081 0.0067 0.0001 0.0028 0.0089 0.0264 0.0008 0.0003 0.0001 0.0003 0 0.0001 0
Lipid Particles 0.0232 0.0005 0.0152 0.0102 0.0296 0.0143 0.019 0.0004 0.0033 0.0075 0.0398 0.0017 0.0007 0.0001 0.0006 0 0.0004 0.0002
Mitochondria 0.014 0.0013 0.0114 0.0227 0.0242 0.0155 0.0024 0.0005 0.0029 0.0019 0.0163 0.0038 0.001 0.0004 0.0008 0.0002 0.0004 0.0003
None 0.1952 0.38 0.2069 0.1068 0.2727 0.2145 0.2106 0.278 0.2615 0.2038 0.1601 0.265 0.1816 0.1599 0.176 0.0624 0.194 0.1473
Nuclear Periphery 0.0459 0.0124 0.0176 0.0154 0.0254 0.0096 0.008 0.0119 0.0077 0.0108 0.0242 0.0073 0.0129 0.0079 0.0138 0.0045 0.0068 0.0056
Nucleolus 0.0097 0.0103 0.0085 0.0073 0.0068 0.0302 0.0061 0.0098 0.0077 0.0076 0.0082 0.0168 0.0112 0.0121 0.0126 0.0107 0.035 0.0267
Nucleus 0.3424 0.4329 0.5118 0.6624 0.3622 0.506 0.5124 0.5271 0.5157 0.4044 0.3517 0.5502 0.6615 0.6447 0.6594 0.8511 0.6098 0.6825
Peroxisomes 0.0163 0.002 0.0133 0.0188 0.0541 0.0062 0.0238 0.0002 0.0044 0.0042 0.0407 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Punctate Nuclear 0.0626 0.0625 0.0733 0.048 0.0891 0.0818 0.0385 0.0451 0.05 0.1987 0.0739 0.064 0.0477 0.063 0.0631 0.0447 0.0784 0.0712
Vacuole 0.0065 0.0014 0.0038 0.0007 0.0019 0.0051 0.001 0.0019 0.0013 0.0007 0.0053 0.0039 0.0013 0.0013 0.001 0.0002 0.0009 0.0008
Vacuole Periphery 0.0034 0.0003 0.0014 0.0005 0.002 0.0015 0.0005 0.0003 0.0002 0.0004 0.0018 0.0012 0.0004 0.0001 0.0004 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1311 13.5263 15.4416 19.8158 9.809 17.4493 19.4625 18.4613 20.0145 21.5343
Translational Efficiency 0.9382 0.7214 0.7389 0.4789 1.1764 0.5872 0.5551 0.7059 0.6662 0.5296

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
172 193 2009 1351 2417 1940 1508 1084 2589 2133 3517 2435

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 714.62 784.31 799.44 858.99 682.70 756.10 879.50 870.83 684.82 758.65 833.77 864.26
Standard Deviation 72.97 99.57 106.89 132.71 79.98 90.22 108.83 132.97 79.93 91.47 114.79 132.95
Intensity Change Log 2 — 0.134248 0.161813 0.265465 — 0.147325 0.365432 0.351139 — 0.140652 0.264888 0.307959

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.000418 0.000488 0.003686 0.000038 0.000407 0.000579 0.001900 0.000041 0.000408 0.000527 0.002891
Bud Neck 0.002603 0.009594 0.018067 0.026232 0.005187 0.016775 0.008942 0.017526 0.005015 0.016125 0.014154 0.022356
Bud Site 0.000533 0.002955 0.006468 0.028057 0.001723 0.010249 0.002940 0.039937 0.001644 0.009589 0.004955 0.033346
Cell Periphery 0.000094 0.000112 0.000145 0.000235 0.000122 0.000190 0.000184 0.000378 0.000120 0.000183 0.000161 0.000299
Cytoplasm 0.355697* 0.265798* 0.073170 0.123388* 0.396145* 0.353308* 0.074075 0.149353* 0.393458* 0.345390* 0.073558 0.134947*
Cytoplasmic Foci 0.077497 0.106187 0.000954 0.006705 0.058050 0.085783 0.004081 0.009503 0.059342 0.087630 0.002295 0.007951
Eisosomes 0.000061 0.000081 0.000018 0.000044 0.000046 0.000068 0.000042 0.000042 0.000047 0.000069 0.000028 0.000043
Endoplasmic Reticulum 0.000673 0.002223 0.003257 0.007950 0.000614 0.001645 0.004213 0.007798 0.000618 0.001698 0.003667 0.007882
Endosome 0.002668 0.006465 0.000612 0.010184 0.001789 0.005846 0.001272 0.010372 0.001847 0.005902 0.000895 0.010268
Golgi 0.000137 0.001233 0.000108 0.007756 0.000321 0.001698 0.000119 0.005566 0.000309 0.001656 0.000113 0.006781
Lipid Particles 0.004559 0.004009 0.000250 0.002365 0.003683 0.005000 0.001064 0.001419 0.003742 0.004911 0.000599 0.001944
Mitochondria 0.000497 0.002037 0.001800 0.005578 0.001661 0.005591 0.001481 0.008515 0.001584 0.005269 0.001663 0.006885
Mitotic Spindle 0.000087 0.003740 0.005779 0.064472 0.001570 0.013836 0.005623 0.064292 0.001472 0.012923 0.005712 0.064392
None 0.005495 0.005291 0.008075 0.009361 0.006095 0.007065 0.012309 0.011291 0.006055 0.006904 0.009890 0.010220
Nuclear Periphery 0.000673 0.001141 0.001341 0.004657 0.001046 0.001250 0.001372 0.003105 0.001021 0.001240 0.001354 0.003966
Nuclear Periphery Foci 0.000335 0.000666 0.000397 0.002903 0.000324 0.000653 0.000603 0.001729 0.000325 0.000654 0.000485 0.002380
Nucleolus 0.003202 0.005198 0.006486 0.004836 0.002793 0.005742 0.009437 0.002243 0.002820 0.005693 0.007751 0.003682
Nucleus 0.530039* 0.532313* 0.867225* 0.634801* 0.506966* 0.453571* 0.860639* 0.620175* 0.508499* 0.460696* 0.864401* 0.628290*
Peroxisomes 0.001405 0.013205 0.000139 0.001111 0.001465 0.006045 0.000343 0.003225 0.001461 0.006692 0.000226 0.002053
Vacuole 0.013518 0.031654 0.004936 0.043425 0.010098 0.024440 0.010449 0.037132 0.010325 0.025092 0.007300 0.040624
Vacuole Periphery 0.000156 0.005681 0.000287 0.012252 0.000263 0.000837 0.000232 0.004499 0.000256 0.001275 0.000264 0.008800

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.94 -17.34 -2.86 -2.22 -2.00 -4.01 -21.31 -10.48 -6.88 -5.07 -4.40 -29.57 -4.96 -3.75 -3.01
Bud Neck -4.04 -14.15 -13.09 -5.92 -0.52 -8.25 -7.71 -9.85 -1.02 -4.21 -8.63 -14.85 -15.34 -3.15 -2.62
Bud Site -5.07 -6.70 -8.98 -7.25 -4.85 -6.71 -3.51 -7.65 -3.74 -6.67 -6.86 -5.87 -11.09 -4.76 -8.07
Cell Periphery -1.43 -2.67 -5.18 -2.73 -1.89 -4.29 -3.63 -3.78 -1.40 -2.10 -4.36 -3.09 -5.09 -0.81 -2.79
Cytoplasm 2.90 12.00 10.41 8.74 -6.23 5.18 49.95 37.37 28.94 -6.03 5.99 55.07 44.74 33.68 -8.67
Cytoplasmic Foci -1.32 7.83 7.62 9.13 -4.95 -7.09 23.53 22.34 24.55 -0.73 -7.30 25.82 24.80 26.87 -3.13
Eisosomes -1.58 5.90 1.11 3.23 -17.25 -6.26 -3.26 -6.30 0.65 -2.92 -6.63 5.29 -6.32 2.32 -13.00
Endoplasmic Reticulum -3.29 -16.36 -20.83 -10.01 -12.79 -4.91 -20.51 -18.85 -16.27 -9.29 -5.60 -25.86 -28.04 -22.44 -15.53
Endosome -1.82 2.92 -1.05 1.35 -5.83 -7.28 1.31 -2.70 1.76 -3.11 -7.53 4.24 -4.06 2.80 -6.10
Golgi -2.12 0.64 -2.71 -1.08 -2.76 -3.98 1.69 -2.48 -0.00 -2.86 -4.21 1.90 -3.36 -0.50 -3.84
Lipid Particles 0.45 4.65 3.40 4.11 -4.40 -2.64 8.02 7.20 8.40 -1.04 -2.52 11.00 7.55 8.78 -4.44
Mitochondria -4.29 -8.49 -9.59 -3.36 -5.12 -5.66 0.51 -3.73 1.29 -3.94 -5.81 -0.38 -5.72 1.86 -6.03
Mitotic Spindle -2.53 -6.34 -10.80 -8.37 -8.44 -5.60 -4.75 -8.89 -5.69 -6.48 -5.79 -6.47 -13.58 -8.42 -10.62
None -0.57 -2.62 -2.73 -1.22 -0.54 -1.17 -3.03 -1.25 -0.29 1.93 -1.23 -3.51 -2.37 -0.99 1.25
Nuclear Periphery -3.95 -9.27 -11.68 -9.50 -9.15 -1.76 -6.35 -10.34 -9.44 -7.24 -2.17 -8.26 -14.69 -13.25 -11.70
Nuclear Periphery Foci -2.03 -1.39 -4.50 -1.86 -4.04 -2.54 -4.17 -4.37 -3.52 -1.91 -2.90 -4.04 -6.17 -4.57 -4.06
Nucleolus -1.52 -4.74 -1.15 1.09 4.78 -3.44 -7.59 1.43 5.28 9.21 -3.68 -8.57 -0.53 3.88 9.83
Nucleus -0.03 -12.73 -6.24 -6.94 17.50 5.53 -42.27 -15.56 -18.82 15.27 5.17 -51.10 -21.27 -24.18 23.25
Peroxisomes -2.20 3.74 2.91 2.47 -4.07 -5.61 3.46 0.65 5.28 -1.16 -5.97 4.79 2.20 6.88 -1.85
Vacuole -3.14 2.51 -10.26 -5.59 -16.40 -9.21 -4.86 -14.70 -11.01 -12.40 -9.85 -1.39 -20.89 -14.18 -20.21
Vacuole Periphery -1.13 -1.99 -3.77 0.36 -3.65 -5.45 0.78 -2.55 -1.30 -2.66 -2.42 0.27 -4.36 -2.03 -4.40
Standard name
Human Ortholog
Description Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
Localization
Cell Percentages nucleus (44%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Swd3

Swd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available