Standard name
Human Ortholog
Description Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.13 0 0.1 0 0 0 0 0 0
Bud Neck 0 0 0.12 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Cytoplasm 0.64 0.8 0.88 0.86 0.83 0.78 0.68 0.78 0.7 0.7 0.61 0.64 0.92 0.92 0.95 0.1 0 0.05 0.32 0.46 0.31 0.4 0.45 0.48
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.24 0.18 0.05 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.46 0.21 0 0.05 0 0.16 0.34 0.2 0.36 0.36 0.53 0.38 0 0 0 0.16 0.11 0.08 0.42 0.32 0.4 0.39 0.29 0.24
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.28 0.29 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0.08 0.05 0.07 0.07 0.05 0.05 0 0.07 0 0 0 0.28 0.37 0.22 0 0 0.05 0 0.08 0.08
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.18 0.14 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 2 2 0 1 0 0 0 0 1 1 2 7 0 4 0 2 6 5 4 6 14 12
Bud 3 2 5 3 6 3 6 8 9 11 14 10 5 2 0 12 3 10 1 2 1 1 4 11
Bud Neck 1 3 13 6 20 15 13 16 12 12 8 13 11 14 8 0 0 1 1 5 1 3 5 11
Bud Site 0 0 0 1 0 0 2 7 6 5 2 1 0 0 0 2 3 4 – – – – – –
Cell Periphery 0 1 1 1 3 0 0 1 1 2 2 1 1 4 0 2 2 7 0 0 1 3 6 6
Cytoplasm 90 138 99 158 269 234 252 323 307 230 181 188 328 394 503 9 3 5 50 90 42 104 211 221
Endoplasmic Reticulum 4 2 1 1 1 3 0 1 0 0 0 2 1 2 0 2 1 2 1 0 0 2 4 3
Endosome 0 0 0 0 3 3 0 0 1 0 0 1 2 2 7 20 19 19 7 7 9 10 11 12
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 2 2 2 0 1 0 1 2
Mitochondria 65 37 4 10 13 48 127 83 156 117 157 111 4 0 1 15 9 8 65 62 54 101 134 113
Nucleus 0 0 2 1 2 0 2 2 4 1 1 3 1 2 0 1 1 5 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 2
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 4 0 0 0 0 2 0
Peroxisomes 0 0 0 1 2 0 0 0 1 0 0 1 0 1 1 19 22 30 1 1 2 0 5 3
SpindlePole 2 3 3 9 27 16 27 29 23 16 9 21 16 19 12 26 29 23 6 6 6 9 36 36
Vac/Vac Membrane 0 3 1 0 6 3 1 0 2 1 0 1 3 2 4 7 14 14 6 10 3 2 12 15
Unique Cell Count 141 173 112 184 324 299 372 416 436 328 296 294 356 430 528 93 79 103 157 197 136 260 468 465
Labelled Cell Count 167 191 131 193 352 326 430 470 522 396 377 354 375 449 536 122 109 136 157 197 136 260 468 465


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.8 3.8 4.4 4.2 4.4 3.5 3.2 3.5 2.9 3.1 2.8 3.2 4.7 4.8 4.9 6.4 6.8 6.7 4.6 5.0 5.0
Std Deviation (1e-4) 0.4 0.6 1.2 1.6 1.4 0.8 1.2 0.9 0.7 1.0 0.8 1.1 1.0 1.0 1.2 1.3 1.1 1.6 1.2 4.8 1.4
Intensity Change (Log2) – – – -0.06 -0.01 -0.34 -0.48 -0.33 -0.6 -0.51 -0.67 -0.44 0.1 0.12 0.16 0.53 0.63 0.61 0.05 0.18 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 4.3 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0796 -0.5165 -0.7898 -0.6759 -0.9989 -0.5823 0.0332 -0.105 -0.0916 -0.6501 -0.501 -0.5152 -1.1978 -0.8956 -1.0201 -1.215 -1.2648 -0.9317
Actin 0.0123 0.0106 0.0176 0.0029 0.0415 0.0107 0.0634 0.0077 0.0107 0.0027 0.0099 0.011 0.0123 0.0174 0.0073 0.0102 0.0154 0.0084
Bud 0.0008 0.0069 0.0025 0.002 0.0019 0.0016 0.003 0.0021 0.0074 0.0021 0.0033 0.0024 0.0043 0.0021 0.0072 0.0033 0.0027 0.0027
Bud Neck 0.0011 0.0022 0.0036 0.005 0.0028 0.0084 0.0067 0.003 0.006 0.0013 0.0008 0.0165 0.0018 0.0029 0.0028 0.0024 0.001 0.0086
Bud Periphery 0.0032 0.0083 0.0038 0.0052 0.0068 0.0022 0.0037 0.0049 0.0135 0.0084 0.0133 0.0047 0.0047 0.0039 0.0062 0.0083 0.0067 0.0027
Bud Site 0.0024 0.0384 0.0079 0.0054 0.0122 0.0009 0.015 0.0239 0.0118 0.0038 0.0036 0.0032 0.0068 0.0266 0.0123 0.0147 0.0017 0.0007
Cell Periphery 0.0011 0.0018 0.0011 0.0005 0.0007 0.0009 0.0028 0.0015 0.0021 0.0026 0.0006 0.0007 0.0012 0.0018 0.0035 0.0008 0.0005 0.0005
Cytoplasm 0.0231 0.0361 0.0441 0.0358 0.0168 0.0307 0.0317 0.0531 0.0407 0.0408 0.0292 0.0521 0.0353 0.047 0.0366 0.0302 0.0525 0.0488
Cytoplasmic Foci 0.1454 0.1434 0.1344 0.1212 0.1348 0.1315 0.1559 0.1629 0.1129 0.0859 0.1268 0.1401 0.1419 0.1453 0.1362 0.1163 0.1393 0.1455
Eisosomes 0.0013 0.0006 0.0006 0.0003 0.0011 0.0007 0.0017 0.001 0.001 0.001 0.0007 0.0004 0.0012 0.002 0.0017 0.0007 0.0004 0.0005
Endoplasmic Reticulum 0.0043 0.0018 0.0022 0.0044 0.0016 0.0016 0.004 0.0041 0.0033 0.0019 0.001 0.0016 0.0087 0.0045 0.0021 0.0027 0.0032 0.0033
Endosome 0.1301 0.115 0.1057 0.0341 0.075 0.0749 0.1336 0.0767 0.0815 0.048 0.0808 0.0771 0.1461 0.1356 0.1243 0.0765 0.2014 0.11
Golgi 0.0351 0.0505 0.0315 0.0339 0.0141 0.0354 0.0469 0.0402 0.0319 0.0311 0.0351 0.036 0.0619 0.0759 0.0477 0.0414 0.0742 0.0613
Lipid Particles 0.0489 0.0279 0.0526 0.0207 0.0531 0.0452 0.0779 0.0307 0.0304 0.0036 0.0491 0.0426 0.0669 0.0376 0.036 0.0309 0.0095 0.0352
Mitochondria 0.216 0.289 0.2908 0.4283 0.4191 0.3107 0.1208 0.2086 0.2952 0.2254 0.3205 0.2166 0.2673 0.2555 0.3036 0.3824 0.1998 0.3637
None 0.2743 0.1499 0.1856 0.2277 0.0818 0.195 0.2243 0.2664 0.2229 0.4529 0.2136 0.2653 0.1364 0.1455 0.1699 0.1281 0.1827 0.1063
Nuclear Periphery 0.0015 0.0008 0.0012 0.0024 0.0012 0.0025 0.0084 0.0022 0.0059 0.001 0.0007 0.0018 0.0048 0.0018 0.0014 0.0031 0.0014 0.0008
Nucleolus 0.0013 0.0023 0.0015 0.001 0.0029 0.0016 0.0047 0.0014 0.0022 0.0025 0.0009 0.0025 0.0042 0.0017 0.0024 0.0008 0.0005 0.0006
Nucleus 0.001 0.0024 0.0014 0.0015 0.0016 0.0016 0.0028 0.0013 0.0044 0.0035 0.001 0.0045 0.002 0.002 0.0019 0.0008 0.001 0.0009
Peroxisomes 0.0843 0.0961 0.0943 0.0569 0.1172 0.1278 0.0699 0.0957 0.0969 0.0723 0.0963 0.0983 0.0726 0.0766 0.081 0.1361 0.0873 0.0878
Punctate Nuclear 0.0017 0.0049 0.005 0.0023 0.0033 0.0083 0.0088 0.0028 0.0041 0.0031 0.0021 0.0095 0.0041 0.0023 0.0029 0.0024 0.0008 0.0026
Vacuole 0.0084 0.008 0.0091 0.0058 0.0069 0.0051 0.011 0.0077 0.0111 0.0044 0.0074 0.0092 0.0114 0.0094 0.0097 0.0042 0.0133 0.0054
Vacuole Periphery 0.0024 0.0029 0.0035 0.0027 0.0036 0.0026 0.0028 0.0021 0.0043 0.0016 0.0034 0.004 0.004 0.0026 0.0035 0.0034 0.0049 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5135 7.3532 17.9281 14.516 12.9502 12.9188 10.8867 18.6382 14.5935 14.4938
Translational Efficiency 1.4358 2.2295 0.8954 1.3591 1.3836 1.5858 2.1412 0.9493 1.3195 1.3624

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1297 505 2277 925 1244 1892 199 1502 2541 2397 2476 2427

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.12 725.01 814.35 753.01 623.86 711.67 873.60 794.06 624.50 714.48 819.11 778.41
Standard Deviation 67.16 92.40 89.44 103.98 69.83 86.44 95.06 107.27 68.49 87.90 91.34 107.89
Intensity Change Log 2 — 0.213868 0.381516 0.268536 — 0.189986 0.485751 0.348026 — 0.201988 0.434522 0.308788

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000344 0.001233 0.001330 0.001608 0.000250 0.000611 0.001120 0.000801 0.000298 0.000742 0.001313 0.001109
Bud Neck 0.049210 0.067691 0.031179 0.008219 0.039631 0.064547 0.005544 0.012418 0.044521 0.065209 0.029119 0.010818
Bud Site 0.009763 0.042053 0.024111 0.033364 0.007081 0.022699 0.010983 0.039878 0.008450 0.026777 0.023056 0.037395
Cell Periphery 0.000188 0.000334 0.000140 0.000221 0.000220 0.000216 0.000232 0.000220 0.000203 0.000241 0.000147 0.000221
Cytoplasm 0.234683* 0.166273* 0.393214* 0.442009* 0.258937* 0.201147* 0.316696* 0.476768* 0.246557* 0.193800* 0.387064* 0.463521*
Cytoplasmic Foci 0.370020* 0.336590* 0.061658 0.066463 0.358221* 0.316222* 0.099275 0.051404 0.364244* 0.320513* 0.064681 0.057143
Eisosomes 0.000311 0.000268 0.000056 0.000091 0.000280 0.000212 0.000135 0.000066 0.000296 0.000223 0.000062 0.000076
Endoplasmic Reticulum 0.000679 0.000503 0.003447 0.002320 0.001580 0.000661 0.004161 0.001822 0.001120 0.000628 0.003504 0.002012
Endosome 0.040707 0.063948 0.006981 0.011088 0.036662 0.061121 0.014939 0.006108 0.038727 0.061717 0.007621 0.008006
Golgi 0.018383 0.031001 0.001794 0.003742 0.018609 0.028361 0.003701 0.003116 0.018494 0.028917 0.001947 0.003354
Lipid Particles 0.021959 0.008614 0.002877 0.003439 0.030399 0.014585 0.007974 0.001839 0.026091 0.013327 0.003286 0.002449
Mitochondria 0.066955 0.060753 0.007184 0.005241 0.063380 0.083405 0.001657 0.001948 0.065205 0.078633 0.006739 0.003203
Mitotic Spindle 0.002897 0.008723 0.024065 0.016992 0.001753 0.012544 0.002601 0.018482 0.002337 0.011739 0.022340 0.017914
None 0.002310 0.001599 0.002606 0.004693 0.002393 0.003847 0.003554 0.003555 0.002351 0.003373 0.002682 0.003989
Nuclear Periphery 0.000412 0.000182 0.002575 0.001263 0.000979 0.000348 0.001573 0.001214 0.000690 0.000313 0.002494 0.001233
Nuclear Periphery Foci 0.000225 0.000275 0.000885 0.001202 0.000530 0.000298 0.002947 0.000889 0.000374 0.000293 0.001051 0.001009
Nucleolus 0.002654 0.002046 0.002117 0.001094 0.002152 0.002829 0.001844 0.000598 0.002408 0.002664 0.002095 0.000787
Nucleus 0.083449 0.054471 0.357954* 0.240563* 0.095360 0.063706 0.365770* 0.262158* 0.089280 0.061760 0.358582* 0.253927*
Peroxisomes 0.049073 0.099785 0.016141 0.011905 0.034130 0.055020 0.040271 0.007424 0.041758 0.064452 0.018080 0.009132
Vacuole 0.042358 0.049176 0.058808 0.142145 0.043298 0.061955 0.114409 0.107230 0.042818 0.059263 0.063277 0.120537
Vacuole Periphery 0.003419 0.004481 0.000878 0.002337 0.004155 0.005666 0.000615 0.002061 0.003779 0.005416 0.000857 0.002166

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.02 -6.16 -2.94 -0.03 0.25 -3.35 -2.50 -3.83 -0.50 1.12 -4.59 -6.82 -4.41 -0.95 2.04
Bud Neck -2.63 5.33 14.11 10.06 12.39 -6.35 12.60 10.25 17.00 -3.81 -6.22 6.08 16.82 20.27 10.64
Bud Site -6.33 -6.44 -4.29 3.16 -0.13 -10.17 -0.97 -7.66 -1.80 -4.94 -11.28 -7.41 -8.55 0.16 -1.77
Cell Periphery -2.60 2.65 0.54 2.77 -1.90 0.26 -0.74 2.04 1.64 2.35 -1.84 3.72 1.78 3.04 -1.89
Cytoplasm 5.84 -15.39 -13.63 -16.90 -1.64 6.50 -1.34 -15.30 -23.42 -6.64 7.73 -15.91 -21.12 -28.32 -4.83
Cytoplasmic Foci 2.75 44.82 43.76 25.52 1.17 4.87 24.60 45.36 47.25 5.21 6.52 58.04 63.86 53.33 4.92
Eisosomes 1.00 8.17 7.10 5.10 -6.51 4.49 7.29 15.70 15.58 6.34 3.76 13.43 12.64 14.18 -4.10
Endoplasmic Reticulum 1.84 -15.50 -11.03 -11.79 4.08 2.35 -5.40 -2.36 -12.48 4.68 2.47 -10.96 -6.97 -16.81 6.38
Endosome -4.27 14.27 13.15 11.43 -1.51 -7.39 7.36 13.98 22.90 5.07 -8.46 18.54 19.54 25.29 2.18
Golgi -3.34 11.58 9.70 8.31 -0.56 -4.32 8.56 10.61 15.61 0.84 -5.70 15.69 14.65 17.59 -0.38
Lipid Particles 6.68 13.25 12.91 3.87 -0.76 6.98 9.48 13.71 14.48 5.94 8.66 17.95 18.82 14.44 5.06
Mitochondria 0.93 13.36 13.86 8.55 2.76 -3.33 14.23 14.33 20.87 -0.04 -2.80 18.65 20.09 22.32 5.69
Mitotic Spindle -3.17 -9.15 -4.53 -1.59 3.20 -6.93 -1.15 -6.29 -1.06 -5.81 -7.24 -9.61 -7.62 -1.40 2.78
None 1.57 -0.90 -1.94 -2.81 -1.66 -1.70 -1.38 -1.95 0.76 0.10 -1.55 -1.20 -2.82 -0.03 -2.28
Nuclear Periphery 4.53 -13.99 -13.50 -16.32 5.73 2.23 -3.16 -2.89 -15.32 0.80 2.63 -10.33 -7.42 -20.99 6.10
Nuclear Periphery Foci -1.11 -9.58 -8.02 -6.49 -2.76 1.41 -3.40 -3.36 -8.58 2.95 0.62 -6.71 -8.28 -10.70 0.45
Nucleolus 0.40 0.37 3.25 2.16 5.25 -1.81 -0.65 4.87 6.24 2.25 -1.12 -0.14 5.45 6.40 6.73
Nucleus 3.65 -39.96 -19.47 -19.79 11.17 6.18 -13.04 -25.42 -31.93 3.83 6.93 -43.26 -32.64 -37.61 12.46
Peroxisomes -6.44 10.98 11.99 12.13 2.55 -6.05 0.30 11.03 18.44 2.97 -7.23 10.87 16.84 21.48 5.55
Vacuole -1.88 -8.75 -21.46 -18.55 -17.46 -5.57 -8.20 -22.03 -18.79 -1.10 -6.28 -11.80 -30.66 -26.11 -21.08
Vacuole Periphery -1.23 9.24 4.28 3.58 -1.36 -2.84 8.76 5.09 8.21 -1.41 -4.11 11.98 6.52 9.55 -1.49
Standard name
Human Ortholog
Description Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
Localization
Cell Percentages mitochondrion (28%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ecm31

Ecm31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available