Standard name | |
---|---|
Human Ortholog | |
Description | Acyl-coenzymeA:ethanol O-acyltransferase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; possesses short-chain esterase activity; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 4 | 23 | 37 | 25 | 15 | 47 | 35 | 37 | 55 | 45 | 41 | 17 | 26 | 16 | 2 | 4 | 0 | 4 | 1 | 6 | 4 | 9 | 12 |
Bud | 1 | 0 | 2 | 3 | 4 | 0 | 5 | 4 | 3 | 11 | 10 | 4 | 5 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 0 | 3 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 5 | 34 | 44 | 34 | 20 | 24 | 14 | 8 | 5 | 4 | 4 | 31 | 48 | 46 | 1 | 6 | 3 | 1 | 0 | 5 | 0 | 1 | 2 |
Cytoplasm | 80 | 78 | 38 | 64 | 90 | 67 | 74 | 65 | 68 | 62 | 56 | 65 | 156 | 166 | 165 | 13 | 58 | 30 | 11 | 21 | 8 | 63 | 87 | 47 |
Endoplasmic Reticulum | 309 | 164 | 423 | 274 | 326 | 355 | 369 | 353 | 280 | 311 | 179 | 208 | 341 | 351 | 559 | 0 | 1 | 0 | 401 | 209 | 494 | 373 | 542 | 620 |
Endosome | 1 | 1 | 1 | 0 | 2 | 2 | 0 | 2 | 2 | 3 | 1 | 3 | 9 | 5 | 11 | 0 | 2 | 1 | 3 | 2 | 2 | 3 | 5 | 7 |
Golgi | 2 | 1 | 14 | 17 | 4 | 4 | 7 | 30 | 33 | 41 | 19 | 36 | 16 | 21 | 27 | 4 | 5 | 1 | 5 | 4 | 5 | 4 | 3 | 6 |
Mitochondria | 5 | 2 | 4 | 29 | 12 | 36 | 71 | 43 | 64 | 135 | 144 | 136 | 0 | 1 | 0 | 0 | 2 | 0 | 3 | 1 | 9 | 5 | 9 | 6 |
Nucleus | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 4 | 2 | 2 | 3 | 4 | 3 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 4 | 3 | 5 | 5 | 12 | 12 | 13 | 20 | 9 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 4 | 2 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 13 | 7 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 4 | 7 | 20 | 20 | 34 | 38 | 80 | 84 | 101 | 100 | 112 | 46 | 108 | 88 | 4 | 7 | 8 | 13 | 6 | 3 | 28 | 26 | 28 |
Unique Cell Count | 400 | 234 | 481 | 393 | 444 | 467 | 522 | 559 | 482 | 604 | 465 | 536 | 522 | 618 | 781 | 24 | 82 | 43 | 452 | 252 | 538 | 501 | 703 | 753 |
Labelled Cell Count | 429 | 261 | 550 | 492 | 525 | 539 | 648 | 648 | 594 | 760 | 577 | 639 | 624 | 726 | 915 | 26 | 87 | 44 | 452 | 252 | 538 | 501 | 703 | 753 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.6 | 16.8 | 13.6 | 13.1 | 13.8 | 13.6 | 13.3 | 13.9 | 14.4 | 13.4 | 13.8 | 13.8 | 16.7 | 16.6 | 15.7 | 17.4 | 15.6 | 16.2 | 15.9 | 16.6 | 16.8 |
Std Deviation (1e-4) | 1.8 | 3.8 | 2.1 | 2.2 | 2.5 | 2.3 | 2.5 | 2.7 | 2.8 | 2.7 | 3.0 | 3.0 | 2.9 | 2.7 | 2.5 | 5.2 | 3.5 | 4.3 | 2.9 | 2.8 | 2.9 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.03 | 0.0 | -0.03 | 0.04 | 0.09 | -0.01 | 0.02 | 0.03 | 0.3 | 0.29 | 0.21 | 0.36 | 0.2 | 0.26 | 0.23 | 0.29 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.7 | 0.6 | -1.2 | 2.6 | 1.0 | 1.9 | 2.7 | 2.9 | 1.9 | -1.2 | -0.5 | -2.7 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.1 | 0.3 | -1.9 | -1.7 | -3.5 | -4.1 | -5.5 | -4.9 | -5.3 | -0.7 | 0.4 | -0.8 | 0 | 0.1 | 0 |
Cytoplasm | 3.8 | 5.4 | 3.2 | 3.2 | 2.0 | 3.1 | 1.3 | 2.1 | 2.2 | 8.8 | 8.0 | 6.2 | 0 | 14.0 | 11.6 |
Endoplasmic Reticulum | -6.7 | -5.6 | -4.8 | -6.7 | -9.2 | -10.4 | -12.8 | -15.8 | -16.1 | -8.4 | -11.2 | -6.8 | -11.4 | -16.8 | -14.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.1 | -2.2 | -2.3 | -1.7 | 2.0 | 2.8 | 2.9 | 1.0 | 2.8 | 0.1 | 0.5 | 0.5 | 0 | 0 | 0 |
Mitochondria | 5.1 | 2.2 | 5.3 | 7.7 | 5.3 | 7.5 | 10.5 | 12.8 | 11.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1.9 | 1.7 | 2.2 | 2.8 | 1.5 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.1 | 2.8 | 4.4 | 4.5 | 7.5 | 8.5 | 8.3 | 9.7 | 9.6 | 5.2 | 8.6 | 6.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.3156 | 23.1434 | 22.46 | 22.156 | 20.772 | 22.8436 | 22.391 | 23.8869 | 22.4468 | 21.4567 | 21.7506 | 22.991 | 22.5321 | 21.3522 | 21.4784 | 21.6112 | 17.7345 | 21.1456 |
Actin | 0.0263 | 0.0023 | 0.0015 | 0.0017 | 0.0011 | 0.0036 | 0.0557 | 0.0022 | 0.0114 | 0.037 | 0.0011 | 0.0013 | 0.0015 | 0.0003 | 0.0137 | 0.0005 | 0.0193 | 0.0003 |
Bud | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0017 | 0 |
Bud Neck | 0.0005 | 0.0002 | 0.0011 | 0.0023 | 0.0008 | 0.0008 | 0.0008 | 0.0004 | 0.0015 | 0.0016 | 0.0057 | 0.0017 | 0.0001 | 0.0002 | 0.001 | 0.0006 | 0.0012 | 0.0002 |
Bud Periphery | 0.0017 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0009 | 0.0023 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0042 | 0 |
Bud Site | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0031 | 0.0006 | 0.0026 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.002 | 0 |
Cell Periphery | 0.0019 | 0.002 | 0.0025 | 0.0012 | 0.005 | 0.0005 | 0.0024 | 0.0028 | 0.0032 | 0.0008 | 0.0009 | 0.0014 | 0.0013 | 0.0039 | 0.0019 | 0.0029 | 0.0018 | 0.0006 |
Cytoplasm | 0.0292 | 0.1297 | 0.0819 | 0.0907 | 0.062 | 0.0538 | 0.0191 | 0.0384 | 0.0472 | 0.0301 | 0.101 | 0.0101 | 0.0093 | 0.0359 | 0.0208 | 0.0031 | 0.0216 | 0.0047 |
Cytoplasmic Foci | 0.0114 | 0.0055 | 0.004 | 0.003 | 0.0102 | 0.0055 | 0.0228 | 0.0038 | 0.0042 | 0.0125 | 0.0052 | 0.0033 | 0.0031 | 0.0028 | 0.0018 | 0.0005 | 0.0018 | 0.0007 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.6597 | 0.7121 | 0.8156 | 0.8564 | 0.7436 | 0.8229 | 0.686 | 0.8688 | 0.8126 | 0.835 | 0.7289 | 0.8908 | 0.8364 | 0.8674 | 0.8993 | 0.9234 | 0.8012 | 0.9256 |
Endosome | 0.0471 | 0.0141 | 0.0163 | 0.0133 | 0.0981 | 0.0271 | 0.0403 | 0.0118 | 0.0189 | 0.0151 | 0.0164 | 0.0106 | 0.0133 | 0.0077 | 0.0084 | 0.0043 | 0.0285 | 0.0073 |
Golgi | 0.0065 | 0.0038 | 0.002 | 0.0015 | 0.0251 | 0.0092 | 0.0112 | 0.0034 | 0.0079 | 0.0084 | 0.0044 | 0.0033 | 0.004 | 0.002 | 0.0044 | 0.0043 | 0.0113 | 0.0013 |
Lipid Particles | 0.0399 | 0.0822 | 0.0113 | 0.0012 | 0.0136 | 0.0119 | 0.0249 | 0.0304 | 0.0313 | 0.0098 | 0.0727 | 0.012 | 0.0263 | 0.0585 | 0.032 | 0.0267 | 0.0183 | 0.0171 |
Mitochondria | 0.0059 | 0.0003 | 0.0063 | 0.0003 | 0.0024 | 0.001 | 0.0057 | 0.0006 | 0.0161 | 0.008 | 0.0038 | 0.0146 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0108 | 0.0002 |
None | 0.0026 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0037 | 0.0001 | 0.0003 | 0.0007 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.001 | 0.0001 |
Nuclear Periphery | 0.0923 | 0.0305 | 0.0317 | 0.0202 | 0.0238 | 0.0496 | 0.0738 | 0.0179 | 0.0186 | 0.0184 | 0.0261 | 0.037 | 0.0864 | 0.0101 | 0.0106 | 0.0219 | 0.0427 | 0.0277 |
Nucleolus | 0.0014 | 0 | 0.0002 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0029 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.001 | 0 |
Nucleus | 0.0177 | 0.0034 | 0.0022 | 0.0014 | 0.0011 | 0.0024 | 0.0115 | 0.001 | 0.0072 | 0.0014 | 0.0116 | 0.0013 | 0.0036 | 0.0007 | 0.0003 | 0.001 | 0.0048 | 0.0009 |
Peroxisomes | 0.0018 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0084 | 0.0002 | 0.0008 | 0.0079 | 0.0058 | 0.0001 | 0.0012 | 0 | 0.0002 | 0 | 0.0003 | 0 |
Punctate Nuclear | 0.0077 | 0.0009 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0055 | 0.0002 | 0.0007 | 0.002 | 0.0013 | 0.0005 | 0.0027 | 0.0003 | 0.0001 | 0.0002 | 0.001 | 0.0002 |
Vacuole | 0.0351 | 0.0113 | 0.0179 | 0.0051 | 0.0095 | 0.0074 | 0.0185 | 0.0161 | 0.011 | 0.0042 | 0.0086 | 0.0085 | 0.006 | 0.0086 | 0.0043 | 0.0083 | 0.0155 | 0.0102 |
Vacuole Periphery | 0.0101 | 0.001 | 0.0034 | 0.001 | 0.003 | 0.0028 | 0.0038 | 0.0011 | 0.0023 | 0.0028 | 0.003 | 0.0022 | 0.0034 | 0.0007 | 0.0006 | 0.0015 | 0.0099 | 0.0029 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.5293 | 19.6402 | 26.9887 | 36.041 | 23.2105 | 26.8379 | 26.6615 | 35.493 | 39.2567 | 34.4794 |
Translational Efficiency | 1.449 | 1.6571 | 1.3402 | 1.1756 | 1.8622 | 1.2369 | 1.4155 | 1.1861 | 1.3815 | 1.4697 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2332 | 825 | 2425 | 880 | 2028 | 1928 | 349 | 1428 | 4360 | 2753 | 2774 | 2308 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1137.00 | 1342.95 | 1550.66 | 1615.15 | 1279.70 | 1405.40 | 1781.54 | 1523.74 | 1203.38 | 1386.69 | 1579.71 | 1558.59 |
Standard Deviation | 162.71 | 264.19 | 232.20 | 276.49 | 180.42 | 215.92 | 248.51 | 254.48 | 185.38 | 233.21 | 246.51 | 266.81 |
Intensity Change Log 2 | — | 0.240173 | 0.447650 | 0.506436 | — | 0.135175 | 0.477319 | 0.251811 | — | 0.185527 | 0.463437 | 0.377216 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000700 | 0.001667 | 0.000558 | 0.003015 | 0.000613 | 0.000652 | 0.000881 | 0.005892 | 0.000660 | 0.000956 | 0.000599 | 0.004795 |
Bud Neck | 0.001012 | 0.000770 | 0.001348 | 0.004363 | 0.000351 | 0.000368 | 0.001744 | 0.006649 | 0.000705 | 0.000489 | 0.001398 | 0.005777 |
Bud Site | 0.008700 | 0.013689 | 0.005338 | 0.023503 | 0.004064 | 0.005612 | 0.004929 | 0.030547 | 0.006544 | 0.008032 | 0.005287 | 0.027861 |
Cell Periphery | 0.002346 | 0.002031 | 0.002263 | 0.023016 | 0.001375 | 0.001826 | 0.005593 | 0.011057 | 0.001894 | 0.001887 | 0.002682 | 0.015617 |
Cytoplasm | 0.031629 | 0.014769 | 0.032295 | 0.043771 | 0.016361 | 0.013115 | 0.010830 | 0.074360 | 0.024527 | 0.013610 | 0.029594 | 0.062697 |
Cytoplasmic Foci | 0.026239 | 0.039498 | 0.003979 | 0.011823 | 0.011106 | 0.013225 | 0.001945 | 0.030344 | 0.019200 | 0.021098 | 0.003723 | 0.023282 |
Eisosomes | 0.000131 | 0.000048 | 0.000042 | 0.000163 | 0.000053 | 0.000050 | 0.000080 | 0.000217 | 0.000095 | 0.000049 | 0.000047 | 0.000197 |
Endoplasmic Reticulum | 0.877633* | 0.785074* | 0.854841* | 0.654756* | 0.925124* | 0.902180* | 0.904127* | 0.520135* | 0.899723* | 0.867087* | 0.861042* | 0.571463* |
Endosome | 0.011069 | 0.042718 | 0.021730 | 0.027864 | 0.010505 | 0.019493 | 0.012447 | 0.047044 | 0.010807 | 0.026453 | 0.020562 | 0.039731 |
Golgi | 0.008349 | 0.024024 | 0.003401 | 0.016247 | 0.004712 | 0.008197 | 0.003097 | 0.028451 | 0.006657 | 0.012940 | 0.003363 | 0.023798 |
Lipid Particles | 0.008537 | 0.012140 | 0.014474 | 0.092780 | 0.006478 | 0.007079 | 0.010474 | 0.128346* | 0.007579 | 0.008595 | 0.013971 | 0.114785* |
Mitochondria | 0.004244 | 0.001298 | 0.001053 | 0.001236 | 0.001148 | 0.002940 | 0.000267 | 0.001020 | 0.002804 | 0.002448 | 0.000954 | 0.001103 |
Mitotic Spindle | 0.000113 | 0.000286 | 0.000572 | 0.003415 | 0.001054 | 0.000242 | 0.000210 | 0.009073 | 0.000550 | 0.000255 | 0.000526 | 0.006916 |
None | 0.004274 | 0.000665 | 0.001379 | 0.000703 | 0.001210 | 0.001649 | 0.000295 | 0.000782 | 0.002849 | 0.001354 | 0.001242 | 0.000752 |
Nuclear Periphery | 0.000186 | 0.000918 | 0.002481 | 0.000886 | 0.000597 | 0.000391 | 0.002122 | 0.000981 | 0.000377 | 0.000549 | 0.002436 | 0.000945 |
Nuclear Periphery Foci | 0.002519 | 0.011629 | 0.018382 | 0.023518 | 0.003767 | 0.003907 | 0.015640 | 0.036236 | 0.003099 | 0.006221 | 0.018037 | 0.031387 |
Nucleolus | 0.000090 | 0.000139 | 0.000060 | 0.000077 | 0.000026 | 0.000026 | 0.000007 | 0.000101 | 0.000060 | 0.000060 | 0.000054 | 0.000092 |
Nucleus | 0.000807 | 0.000630 | 0.001042 | 0.001244 | 0.000382 | 0.000243 | 0.000501 | 0.002780 | 0.000609 | 0.000359 | 0.000974 | 0.002195 |
Peroxisomes | 0.001893 | 0.000429 | 0.000175 | 0.000839 | 0.000688 | 0.000494 | 0.000047 | 0.000759 | 0.001333 | 0.000475 | 0.000159 | 0.000790 |
Vacuole | 0.005036 | 0.032919 | 0.032034 | 0.060706 | 0.007287 | 0.013292 | 0.022797 | 0.060171 | 0.006083 | 0.019173 | 0.030872 | 0.060375 |
Vacuole Periphery | 0.004494 | 0.014658 | 0.002552 | 0.006075 | 0.003096 | 0.005017 | 0.001969 | 0.005054 | 0.003844 | 0.007906 | 0.002479 | 0.005443 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.45 | 0.55 | -2.82 | -1.34 | -2.96 | -0.24 | -1.00 | -5.35 | -5.30 | -4.79 | -1.26 | 0.18 | -6.02 | -5.39 | -6.01 |
Bud Neck | 0.75 | -1.22 | -5.46 | -6.10 | -5.12 | -0.20 | -3.65 | -10.08 | -10.07 | -6.97 | 1.31 | -3.37 | -11.02 | -11.85 | -9.49 |
Bud Site | -2.15 | 2.43 | -5.73 | -3.26 | -7.01 | -1.44 | -0.60 | -11.41 | -10.45 | -9.53 | -1.40 | 1.09 | -12.10 | -10.68 | -12.36 |
Cell Periphery | 1.27 | 0.28 | -10.00 | -10.16 | -10.02 | -1.99 | -3.97 | -8.67 | -8.20 | -3.40 | 0.03 | -3.08 | -12.99 | -12.96 | -12.00 |
Cytoplasm | 7.01 | -0.53 | -3.53 | -7.49 | -3.21 | 2.20 | 1.50 | -14.15 | -15.05 | -12.92 | 8.19 | -3.08 | -13.03 | -16.77 | -10.55 |
Cytoplasmic Foci | -3.31 | 13.49 | 6.58 | 6.96 | -5.24 | -1.30 | 6.32 | -8.95 | -8.01 | -14.18 | -1.11 | 14.23 | -2.61 | -1.25 | -14.84 |
Eisosomes | 4.46 | 4.79 | -1.14 | -5.48 | -5.76 | 0.36 | -1.32 | -8.18 | -8.64 | -4.77 | 4.26 | 4.10 | -5.88 | -10.21 | -9.85 |
Endoplasmic Reticulum | 7.84 | 4.10 | 18.39 | 8.84 | 16.00 | 3.88 | 2.14 | 39.28* | 36.22* | 26.37* | 5.90 | 7.85 | 40.91 | 34.03 | 32.97* |
Endosome | -11.95 | -9.54 | -10.27 | 4.80 | -3.20 | -7.40 | -1.23 | -19.21 | -13.39 | -14.43 | -13.51 | -10.07 | -21.66 | -8.10 | -12.10 |
Golgi | -5.89 | 6.05 | -4.66 | 2.65 | -8.30 | -3.54 | 1.77 | -11.31 | -9.13 | -11.69 | -5.81 | 5.95 | -11.66 | -6.26 | -14.41 |
Lipid Particles | -2.15 | -4.29 | -14.34 | -13.40 | -13.06 | -0.56 | -1.81 | -22.39 | -22.38 | -20.06 | -1.20 | -5.31 | -26.43 | -26.10 | -24.04 |
Mitochondria | 6.05 | 6.91 | 5.69 | 0.16 | -0.44 | -3.23 | 3.52 | 0.65 | 3.53 | -3.24 | 0.79 | 6.15 | 6.15 | 3.30 | -0.58 |
Mitotic Spindle | -3.93 | -1.90 | -3.26 | -3.10 | -2.56 | 1.72 | 1.77 | -5.70 | -6.49 | -6.50 | 1.33 | -0.32 | -6.74 | -7.17 | -6.53 |
None | 5.13 | 4.00 | 4.85 | -0.11 | 2.00 | -1.07 | 2.35 | 1.22 | 2.41 | -1.40 | 3.34 | 3.66 | 4.93 | 2.29 | 1.94 |
Nuclear Periphery | -2.55 | -5.58 | -4.80 | -0.42 | 3.21 | 1.59 | -2.63 | -2.94 | -5.26 | 1.85 | -1.55 | -5.57 | -6.17 | -3.72 | 3.67 |
Nuclear Periphery Foci | -5.62 | -11.41 | -10.11 | -4.92 | -2.25 | -0.24 | -3.68 | -14.26 | -14.18 | -5.45 | -4.74 | -11.73 | -17.50 | -15.04 | -6.85 |
Nucleolus | -0.83 | 0.89 | 0.14 | 0.95 | -0.95 | 0.10 | 1.63 | -2.43 | -2.65 | -3.30 | 0.02 | 0.30 | -1.47 | -1.48 | -1.81 |
Nucleus | 0.67 | -1.46 | -2.22 | -2.47 | -1.63 | 1.77 | -0.61 | -6.76 | -7.43 | -6.00 | 2.44 | -2.77 | -6.62 | -7.68 | -5.28 |
Peroxisomes | 6.93 | 8.67 | 3.25 | -1.42 | -2.43 | 1.48 | 5.32 | -0.40 | -2.47 | -7.93 | 6.73 | 9.70 | 3.37 | -2.45 | -5.31 |
Vacuole | -8.16 | -13.46 | -13.19 | -5.89 | -6.49 | -3.76 | -3.73 | -16.13 | -13.93 | -7.14 | -8.92 | -13.48 | -21.08 | -14.97 | -9.87 |
Vacuole Periphery | -5.61 | 3.03 | -1.42 | 4.21 | -3.26 | -2.59 | 1.12 | -2.35 | 0.09 | -2.76 | -5.40 | 2.80 | -2.37 | 2.90 | -4.59 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|