Standard name | |
---|---|
Human Ortholog | |
Description | Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 6 | 6 | 5 | 11 | 5 | 5 | 1 | 2 | 3 |
Bud | 11 | 3 | 3 | 7 | 8 | 14 | 15 | 15 | 17 | 15 | 15 | 20 | 1 | 1 | 0 | 14 | 4 | 10 | 3 | 0 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 3 | 7 |
Bud Site | 1 | 0 | 0 | 1 | 2 | 8 | 4 | 6 | 5 | 7 | 7 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 17 | 7 | 12 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 5 | 2 | 2 | 3 | 13 | 19 | 4 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 11 | 8 | 15 | 16 | 17 | 24 | 31 | 39 | 20 | 26 | 9 | 7 | 20 | 20 | 24 | 77 | 41 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 7 | 4 | 2 | 0 | 0 | 2 | 1 | 1 | 5 | 1 | 7 | 4 | 5 | 3 | 3 | 12 | 12 | 8 | 1 | 1 | 0 | 0 | 0 | 1 |
Endosome | 30 | 9 | 34 | 8 | 26 | 3 | 2 | 4 | 2 | 1 | 1 | 1 | 8 | 6 | 4 | 31 | 16 | 6 | 27 | 14 | 22 | 8 | 15 | 14 |
Golgi | 9 | 5 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 7 | 5 | 3 | 12 | 9 | 31 | 18 | 13 | 7 | 9 | 7 |
Mitochondria | 389 | 194 | 222 | 158 | 215 | 302 | 413 | 387 | 303 | 425 | 333 | 384 | 33 | 33 | 7 | 43 | 20 | 10 | 367 | 159 | 232 | 74 | 77 | 106 |
Nucleus | 11 | 0 | 3 | 0 | 2 | 10 | 10 | 17 | 8 | 6 | 10 | 5 | 1 | 1 | 1 | 4 | 8 | 2 | 1 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 4 | 2 | 2 | 0 | 1 | 1 | 3 | 3 | 4 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 1 |
Nucleolus | 2 | 0 | 3 | 0 | 1 | 4 | 8 | 6 | 3 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 3 |
Peroxisomes | 23 | 1 | 33 | 6 | 14 | 7 | 6 | 12 | 1 | 2 | 1 | 3 | 4 | 5 | 0 | 31 | 7 | 0 | 25 | 6 | 15 | 7 | 5 | 5 |
SpindlePole | 15 | 0 | 14 | 7 | 8 | 9 | 17 | 12 | 13 | 6 | 4 | 9 | 2 | 2 | 4 | 39 | 22 | 5 | 1 | 1 | 2 | 1 | 3 | 2 |
Vac/Vac Membrane | 90 | 30 | 30 | 4 | 39 | 17 | 25 | 20 | 24 | 27 | 18 | 20 | 15 | 18 | 10 | 16 | 10 | 12 | 21 | 21 | 6 | 6 | 15 | 28 |
Unique Cell Count | 468 | 219 | 287 | 179 | 264 | 332 | 457 | 436 | 327 | 446 | 348 | 395 | 84 | 83 | 59 | 243 | 151 | 71 | 504 | 236 | 312 | 114 | 141 | 190 |
Labelled Cell Count | 625 | 269 | 375 | 210 | 335 | 403 | 535 | 522 | 405 | 524 | 413 | 471 | 101 | 100 | 65 | 290 | 179 | 84 | 504 | 236 | 312 | 114 | 141 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.9 | 5.2 | 4.8 | 4.6 | 3.7 | 3.4 | 3.7 | 3.3 | 3.3 | 3.3 | 3.5 | 6.7 | 6.7 | 6.7 | 5.1 | 5.4 | 6.0 | 6.3 | 6.7 | 6.9 |
Std Deviation (1e-4) | 1.1 | 0.9 | 1.4 | 1.7 | 1.6 | 1.9 | 1.6 | 1.2 | 1.0 | 1.2 | 0.8 | 1.0 | 1.5 | 1.5 | 0.9 | 1.0 | 0.9 | 1.2 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.18 | -0.5 | -0.59 | -0.47 | -0.66 | -0.65 | -0.65 | -0.58 | 0.37 | 0.37 | 0.36 | -0.02 | 0.06 | 0.22 | 0.27 | 0.37 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 3.3 | 0 |
Cytoplasm | 1.6 | 0.6 | 1.0 | 0.9 | 1.9 | 0.5 | 0.3 | -1.7 | -2.5 | 5.1 | 5.2 | 7.8 | 8.0 | 6.5 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -1.2 | -1.1 | 0.3 | -0.4 | -0.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.6 | -6.5 | -9.7 | -13.7 | -12.8 | -10.0 |
Nucleus | 0 | 0 | 1.7 | 1.2 | 2.3 | 1.3 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | -1.4 | -2.7 | 0.4 | -2.4 | -3.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.5 | 0 |
Vacuole | -3.3 | 1.5 | -2.5 | -2.5 | -3.0 | -1.4 | -2.2 | -2.5 | -2.7 | 1.8 | 2.7 | 1.4 | -1.6 | -1.3 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.761 | 1.9613 | 1.631 | 1.3042 | 1.7073 | 1.733 | 4.2183 | 4.3852 | 4.1111 | 3.0193 | 4.6144 | 4.5173 | 4.1252 | 4.1099 | 4.2548 | 4.0453 | 4.142 | 4.2506 |
Actin | 0.0097 | 0.0152 | 0.0174 | 0.0022 | 0.0078 | 0.0038 | 0.0228 | 0.037 | 0.0337 | 0.015 | 0.0486 | 0.0325 | 0.006 | 0.0063 | 0.0112 | 0.0092 | 0.0034 | 0.0068 |
Bud | 0.0012 | 0.0054 | 0.0077 | 0.002 | 0.007 | 0.0007 | 0.0017 | 0.011 | 0.003 | 0.0046 | 0.0074 | 0.0015 | 0.002 | 0.0104 | 0.0053 | 0.0027 | 0.0029 | 0.0013 |
Bud Neck | 0.0021 | 0.0082 | 0.0026 | 0.0311 | 0.0047 | 0.0026 | 0.0082 | 0.0033 | 0.006 | 0.0107 | 0.0013 | 0.0181 | 0.0041 | 0.001 | 0.0033 | 0.0038 | 0.0005 | 0.0116 |
Bud Periphery | 0.0019 | 0.0088 | 0.0178 | 0.006 | 0.0081 | 0.0018 | 0.0043 | 0.019 | 0.0134 | 0.0163 | 0.0538 | 0.0044 | 0.0052 | 0.0138 | 0.0135 | 0.0109 | 0.0114 | 0.0038 |
Bud Site | 0.0077 | 0.0817 | 0.0267 | 0.0114 | 0.0633 | 0.0011 | 0.0357 | 0.0824 | 0.0161 | 0.0093 | 0.0058 | 0.002 | 0.0089 | 0.0257 | 0.0279 | 0.0056 | 0.0016 | 0.0018 |
Cell Periphery | 0.0015 | 0.0025 | 0.0044 | 0.001 | 0.0009 | 0.0003 | 0.008 | 0.0055 | 0.0067 | 0.0064 | 0.0042 | 0.0048 | 0.0011 | 0.0022 | 0.0027 | 0.0008 | 0.0029 | 0.0012 |
Cytoplasm | 0.0214 | 0.0472 | 0.0406 | 0.0044 | 0.0172 | 0.0146 | 0.0305 | 0.0534 | 0.0294 | 0.0379 | 0.047 | 0.034 | 0.016 | 0.0501 | 0.0251 | 0.0092 | 0.0148 | 0.0159 |
Cytoplasmic Foci | 0.0537 | 0.0643 | 0.0596 | 0.03 | 0.1234 | 0.0341 | 0.0835 | 0.0851 | 0.0607 | 0.0575 | 0.0412 | 0.0722 | 0.0569 | 0.0842 | 0.0421 | 0.0584 | 0.0265 | 0.0435 |
Eisosomes | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0013 | 0.0021 | 0.0008 | 0.005 | 0.0007 | 0.0005 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0036 | 0.0084 | 0.0029 | 0.0015 | 0.0018 | 0.0012 | 0.0018 | 0.0019 | 0.0021 | 0.0011 | 0.0011 | 0.0036 | 0.002 | 0.0021 | 0.0019 | 0.0004 | 0.0008 | 0.0008 |
Endosome | 0.2185 | 0.137 | 0.1172 | 0.0906 | 0.1667 | 0.0981 | 0.1021 | 0.1276 | 0.1088 | 0.1024 | 0.0807 | 0.1023 | 0.1275 | 0.1334 | 0.0689 | 0.0683 | 0.0787 | 0.0837 |
Golgi | 0.0801 | 0.0903 | 0.0773 | 0.0742 | 0.0956 | 0.098 | 0.0462 | 0.089 | 0.0754 | 0.0394 | 0.0397 | 0.1004 | 0.0752 | 0.069 | 0.0419 | 0.044 | 0.055 | 0.079 |
Lipid Particles | 0.0374 | 0.0091 | 0.0073 | 0.006 | 0.0231 | 0.0082 | 0.0155 | 0.0122 | 0.0086 | 0.0056 | 0.0131 | 0.0207 | 0.012 | 0.0091 | 0.0053 | 0.0064 | 0.0121 | 0.0082 |
Mitochondria | 0.4837 | 0.4389 | 0.5337 | 0.6769 | 0.3642 | 0.6918 | 0.5106 | 0.3966 | 0.5562 | 0.6262 | 0.4929 | 0.5345 | 0.6106 | 0.5179 | 0.6434 | 0.7242 | 0.7568 | 0.6828 |
None | 0.0044 | 0.0129 | 0.0162 | 0.0011 | 0.002 | 0.0121 | 0.0179 | 0.0155 | 0.0328 | 0.0025 | 0.1008 | 0.0096 | 0.0237 | 0.0142 | 0.0447 | 0.0063 | 0.0097 | 0.0199 |
Nuclear Periphery | 0.0056 | 0.002 | 0.0008 | 0.0006 | 0.0012 | 0.0002 | 0.0042 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0017 | 0.0008 | 0.0009 | 0.0004 | 0.0001 | 0.0006 | 0.0002 |
Nucleolus | 0.0019 | 0.0026 | 0.0009 | 0.0005 | 0.0014 | 0.0003 | 0.0019 | 0.0007 | 0.0006 | 0.0005 | 0.0004 | 0.0007 | 0.001 | 0.0015 | 0.001 | 0.0003 | 0.0003 | 0.0004 |
Nucleus | 0.0017 | 0.0026 | 0.0015 | 0.0008 | 0.0017 | 0.0004 | 0.0026 | 0.0009 | 0.0009 | 0.0008 | 0.0004 | 0.0007 | 0.0008 | 0.0025 | 0.0009 | 0.0004 | 0.0004 | 0.0006 |
Peroxisomes | 0.0356 | 0.0361 | 0.0276 | 0.0425 | 0.084 | 0.0214 | 0.0462 | 0.0443 | 0.0279 | 0.0445 | 0.0493 | 0.041 | 0.0305 | 0.0319 | 0.0473 | 0.0397 | 0.0104 | 0.0296 |
Punctate Nuclear | 0.0017 | 0.0027 | 0.0007 | 0.0001 | 0.0017 | 0.0002 | 0.011 | 0.0004 | 0.0004 | 0.0003 | 0.0009 | 0.0007 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0006 |
Vacuole | 0.0181 | 0.0175 | 0.0278 | 0.009 | 0.0202 | 0.0044 | 0.0295 | 0.0094 | 0.0105 | 0.0113 | 0.0035 | 0.0092 | 0.0099 | 0.0176 | 0.0083 | 0.0051 | 0.0069 | 0.0048 |
Vacuole Periphery | 0.0082 | 0.0061 | 0.0087 | 0.0078 | 0.0039 | 0.0043 | 0.0152 | 0.0033 | 0.0043 | 0.0067 | 0.0026 | 0.0046 | 0.0048 | 0.0053 | 0.0035 | 0.0036 | 0.0041 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3122 | 12.6754 | 16.5312 | 17.9308 | 12.256 | 8.5887 | 16.2143 | 20.1833 | 18.8818 | 17.2013 |
Translational Efficiency | 0.9314 | 0.8622 | 0.7145 | 0.6994 | 0.6693 | 1.2383 | 0.7742 | 0.6072 | 0.6475 | 0.8038 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1127 | 1546 | 182 | 1194 | 1751 | 1817 | 202 | 1300 | 2878 | 3363 | 384 | 2494 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 822.93 | 750.05 | 1096.79 | 1011.07 | 726.80 | 751.60 | 1031.26 | 983.73 | 764.44 | 750.89 | 1062.32 | 996.82 |
Standard Deviation | 104.65 | 92.73 | 133.42 | 141.00 | 89.73 | 96.16 | 127.09 | 144.43 | 106.72 | 94.60 | 134.18 | 143.45 |
Intensity Change Log 2 | — | -0.133783 | 0.414446 | 0.297041 | — | 0.048407 | 0.504778 | 0.436704 | — | -0.045468 | 0.457515 | 0.364227 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005953 | 0.002966 | 0.010567 | 0.008277 | 0.001504 | 0.000502 | 0.010792 | 0.005295 | 0.003246 | 0.001635 | 0.010685 | 0.006723 |
Bud Neck | 0.023489 | 0.023787 | 0.004292 | 0.006778 | 0.007348 | 0.028654 | 0.011882 | 0.005966 | 0.013669 | 0.026416 | 0.008284 | 0.006355 |
Bud Site | 0.135109* | 0.034935 | 0.051490 | 0.105975* | 0.012513 | 0.016172 | 0.092668 | 0.082849 | 0.060520 | 0.024798 | 0.073151 | 0.093920 |
Cell Periphery | 0.001143 | 0.000767 | 0.000435 | 0.000873 | 0.000575 | 0.000329 | 0.001034 | 0.001119 | 0.000798 | 0.000530 | 0.000750 | 0.001001 |
Cytoplasm | 0.026747 | 0.033294 | 0.006138 | 0.017076 | 0.007081 | 0.022155 | 0.008282 | 0.013433 | 0.014782 | 0.027276 | 0.007266 | 0.015177 |
Cytoplasmic Foci | 0.051760 | 0.156927 | 0.134464 | 0.045857 | 0.042108 | 0.108325 | 0.055980 | 0.038872 | 0.045888 | 0.130668 | 0.093178 | 0.042216 |
Eisosomes | 0.000143 | 0.000998 | 0.000136 | 0.000095 | 0.000108 | 0.000140 | 0.000123 | 0.000085 | 0.000122 | 0.000535 | 0.000129 | 0.000090 |
Endoplasmic Reticulum | 0.001625 | 0.000590 | 0.002796 | 0.001906 | 0.000547 | 0.000565 | 0.001666 | 0.001156 | 0.000969 | 0.000577 | 0.002202 | 0.001515 |
Endosome | 0.121672 | 0.077805 | 0.103549 | 0.077718 | 0.061549 | 0.118778 | 0.091909 | 0.090735 | 0.085092 | 0.099942 | 0.097426 | 0.084503 |
Golgi | 0.075481 | 0.126863* | 0.234212* | 0.148849* | 0.113436* | 0.102841 | 0.085762 | 0.116493* | 0.098573 | 0.113884* | 0.156121* | 0.131983* |
Lipid Particles | 0.001891 | 0.004148 | 0.001517 | 0.000488 | 0.003602 | 0.011458 | 0.000387 | 0.000400 | 0.002932 | 0.008097 | 0.000923 | 0.000442 |
Mitochondria | 0.328388* | 0.393643* | 0.220173* | 0.380040* | 0.690576* | 0.463454* | 0.368538* | 0.443190* | 0.548746* | 0.431362* | 0.298220* | 0.412957* |
Mitotic Spindle | 0.029257 | 0.033185 | 0.088541 | 0.063912 | 0.005176 | 0.009802 | 0.118303* | 0.045721 | 0.014606 | 0.020551 | 0.104197* | 0.054430 |
None | 0.000375 | 0.000616 | 0.000241 | 0.000375 | 0.000287 | 0.001175 | 0.000296 | 0.000553 | 0.000322 | 0.000918 | 0.000270 | 0.000468 |
Nuclear Periphery | 0.000157 | 0.000168 | 0.000889 | 0.000225 | 0.000234 | 0.000217 | 0.000265 | 0.000237 | 0.000204 | 0.000194 | 0.000561 | 0.000231 |
Nuclear Periphery Foci | 0.000245 | 0.000279 | 0.002276 | 0.000841 | 0.000181 | 0.000231 | 0.000411 | 0.000326 | 0.000206 | 0.000253 | 0.001295 | 0.000573 |
Nucleolus | 0.000684 | 0.001177 | 0.000480 | 0.000176 | 0.000583 | 0.003057 | 0.000210 | 0.000338 | 0.000623 | 0.002193 | 0.000338 | 0.000260 |
Nucleus | 0.004181 | 0.004695 | 0.004718 | 0.000566 | 0.001105 | 0.008002 | 0.003564 | 0.000614 | 0.002309 | 0.006482 | 0.004111 | 0.000591 |
Peroxisomes | 0.037703 | 0.053770 | 0.030570 | 0.011660 | 0.017015 | 0.037233 | 0.079847 | 0.018195 | 0.025116 | 0.044835 | 0.056492 | 0.015066 |
Vacuole | 0.111975 | 0.034106 | 0.059203 | 0.079205 | 0.014154 | 0.045375 | 0.038140 | 0.082777 | 0.052460 | 0.040195 | 0.048123 | 0.081067 |
Vacuole Periphery | 0.042024 | 0.015280 | 0.043313 | 0.049108 | 0.020318 | 0.021536 | 0.029942 | 0.051645 | 0.028818 | 0.018660 | 0.036279 | 0.050430 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.53 | -1.86 | -1.55 | -4.10 | 0.86 | 1.97 | -3.22 | -3.63 | -5.20 | 1.82 | 2.80 | -3.91 | -3.94 | -6.50 | 1.96 |
Bud Neck | -0.15 | 9.82 | 8.96 | 8.95 | -2.13 | -9.59 | -1.98 | 1.09 | 11.17 | 2.75 | -7.89 | 3.49 | 6.92 | 14.28 | 1.45 |
Bud Site | 15.99* | 8.66 | 3.43 | -12.66 | -6.02 | -2.12 | -7.09 | -15.12 | -14.27 | 0.79 | 12.27 | -1.76 | -7.89 | -19.03 | -2.73 |
Cell Periphery | 1.14 | 3.46 | 1.11 | -0.35 | -2.96 | 1.59 | -1.64 | -1.79 | -3.01 | -0.25 | 1.56 | 0.27 | -1.04 | -2.42 | -1.28 |
Cytoplasm | -2.17 | 9.28 | 3.78 | 6.28 | -6.64 | -8.35 | -0.85 | -4.52 | 4.11 | -2.38 | -7.73 | 5.39 | -0.48 | 7.31 | -5.84 |
Cytoplasmic Foci | -21.61 | -8.34 | 1.81 | 23.70 | 9.04 | -18.24 | -2.19 | 1.28 | 18.50 | 2.69 | -28.45 | -7.78 | 1.85 | 29.58 | 8.40 |
Eisosomes | -3.01 | 0.36 | 5.77 | 3.18 | 2.22 | -3.01 | -1.12 | 2.54 | 7.11 | 3.21 | -3.16 | -0.67 | 4.98 | 3.41 | 3.68 |
Endoplasmic Reticulum | 4.64 | -2.27 | -0.65 | -3.51 | 1.48 | -0.15 | -2.55 | -2.97 | -2.95 | 1.20 | 3.51 | -3.70 | -2.47 | -4.53 | 1.88 |
Endosome | 7.56 | 2.04 | 7.52 | 0.13 | 3.42 | -12.11 | -3.51 | -6.79 | 5.59 | 0.12 | -4.19 | -2.05 | 0.19 | 4.74 | 2.22 |
Golgi | -9.17 | -9.70 | -10.58 | -2.98 | 5.09 | 2.01 | 3.03 | -0.39 | -2.23 | -3.17 | -3.81 | -5.73 | -6.96 | -3.79 | 2.39 |
Lipid Particles | -2.90 | 0.70 | 2.49 | 6.90 | 2.37 | -6.15 | 4.98 | 4.98 | 9.95 | 0.08 | -6.57 | 4.17 | 5.52 | 11.77 | 2.27 |
Mitochondria | -5.20 | 4.49 | -3.58 | 1.19 | -6.51 | 21.42* | 12.46 | 20.41 | 1.70 | -2.80 | 13.46 | 13.70 | 14.18 | 2.19 | -6.12 |
Mitotic Spindle | -1.09 | -4.44 | -7.31 | -6.76 | 1.78 | -4.19 | -7.60 | -11.99 | -10.32 | 4.70 | -3.66 | -8.89 | -14.22 | -11.95 | 4.75 |
None | -3.35 | 3.35 | 0.16 | 3.78 | -4.85 | -6.29 | -0.15 | -2.15 | 3.57 | -2.13 | -7.15 | 1.83 | -2.14 | 4.62 | -3.07 |
Nuclear Periphery | -0.14 | -1.63 | -1.66 | -1.11 | 1.47 | 0.12 | -0.23 | -0.03 | -0.39 | 0.36 | 0.12 | -1.56 | -0.37 | -1.04 | 1.51 |
Nuclear Periphery Foci | -0.31 | -1.61 | -2.17 | -2.00 | 1.11 | -0.78 | -2.32 | -2.18 | -2.19 | 1.07 | -0.81 | -1.84 | -2.70 | -2.36 | 1.19 |
Nucleolus | -2.25 | 0.78 | 2.90 | 5.95 | 1.50 | -4.28 | 1.48 | 0.88 | 4.90 | -0.69 | -4.74 | 1.49 | 2.04 | 6.23 | 0.65 |
Nucleus | -0.08 | -0.04 | 3.43 | 4.90 | 0.97 | -5.55 | -1.12 | 1.25 | 6.07 | 1.26 | -4.74 | -0.87 | 3.48 | 7.67 | 1.56 |
Peroxisomes | -3.74 | 1.21 | 7.67 | 13.31 | 3.67 | -8.33 | -5.67 | -0.50 | 6.86 | 5.51 | -8.59 | -4.78 | 5.25 | 14.29 | 6.40 |
Vacuole | 15.07 | 5.40 | 5.05 | -10.71 | -2.30 | -11.67 | -4.72 | -17.85 | -8.56 | -6.47 | 4.73 | 0.62 | -8.47 | -13.42 | -5.77 |
Vacuole Periphery | 10.70 | -0.23 | -1.93 | -11.47 | -0.82 | -0.74 | -1.79 | -11.70 | -11.11 | -3.80 | 7.50 | -1.78 | -9.89 | -15.87 | -3.18 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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