Standard name
Human Ortholog
Description Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.09 0.12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.05 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.05 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.14 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Golgi 0 0.08 0 0 0 0 0 0 – 0 0 0 0.1 0.24 0.34 – 0.18 0.12 0 0.07 0 0.08 0.12 0.11
Mitochondria 0.93 0.83 0.97 0.98 0.95 0.95 0.94 0.91 – 0.94 0.98 0.96 0.9 0.7 0.61 – 0.09 0 0.85 0.78 0.88 0.7 0.68 0.62
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.05 0 0.05 0.09 0.1 0.07 – 0.11 0.11 0.13 0 0 0 – 0.18 0 0 0 0 0 0 0
Nucleolus 0.1 0 0 0 0.05 0.09 0.11 0.14 – 0.08 0.08 0.07 0.05 0 0 – 0.09 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.05 0.31 0 0 0 0.07 0 0.07
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.21 0.05 0 0.08 0 0 0 – 0 0 0 0.14 0.27 0.25 – 0.27 0.5 0 0.07 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 – 0 0 0 3 0 0 – 0 0 0 1 0 4 3 7
Bud 4 0 8 5 6 16 13 10 – 10 16 17 1 1 2 – 2 2 1 1 0 1 3 1
Bud Neck 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 2 4 4 12 – 6 5 6 0 0 0 – 1 0 – – – – – –
Cell Periphery 3 5 3 5 1 5 7 0 – 1 5 3 1 6 6 – 1 2 1 0 0 0 0 0
Cytoplasm 2 2 0 5 4 4 5 2 – 1 1 2 0 6 5 – 3 0 1 0 0 0 0 0
Endoplasmic Reticulum 1 1 0 0 1 1 2 3 – 5 5 3 3 1 2 – 2 1 0 0 0 0 0 3
Endosome 1 4 7 4 8 0 5 4 – 5 2 0 4 8 6 – 1 0 1 3 10 0 6 7
Golgi 4 11 0 0 4 0 0 0 – 0 0 1 10 47 56 – 4 2 5 11 3 8 22 23
Mitochondria 125 116 282 302 423 387 633 429 – 583 523 544 92 138 101 – 2 0 128 121 254 76 131 136
Nucleus 3 0 3 0 2 7 6 5 – 3 1 3 1 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 5 7 15 13 23 38 69 32 – 71 61 74 3 5 1 – 4 0 0 0 2 0 2 0
Nucleolus 14 4 13 10 21 37 75 65 – 48 43 39 5 2 3 – 2 0 0 1 0 1 0 0
Peroxisomes 1 3 4 4 3 1 3 6 – 4 1 0 2 4 3 – 1 5 2 1 10 8 7 16
SpindlePole 1 0 1 0 1 3 6 5 – 11 5 1 2 2 1 – 1 0 0 0 0 0 0 0
Vac/Vac Membrane 6 29 15 9 34 17 25 21 – 27 21 15 14 53 42 – 6 8 2 10 2 4 10 15
Unique Cell Count 135 140 290 308 447 406 675 469 619 536 566 102 196 166 22 16 151 156 289 108 192 218
Labelled Cell Count 170 183 353 357 533 520 854 594 775 689 708 141 273 228 30 20 151 156 289 108 192 218


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 9.1 5.9 6.0 5.9 5.7 5.3 5.6 – 5.5 5.5 5.6 7.7 8.1 8.4 – 11.2 12.5 9.9 9.7 10.0
Std Deviation (1e-4) 2.1 2.0 1.1 1.2 1.3 1.0 1.2 1.3 – 1.2 1.5 1.4 1.3 1.5 1.3 – 2.5 4.0 1.9 2.4 2.1
Intensity Change (Log2) – – – 0.02 -0.01 -0.06 -0.16 -0.08 – -0.09 -0.1 -0.08 0.4 0.47 0.52 – 0.93 1.08 0.75 0.72 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud -1.0 -1.4 0.8 -0.8 -0.6 – -1.2 0.2 0.2 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Endosome -1.0 -0.6 0 0 0 – 0 0 0 0 1.0 0 – 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 8.8 10.6 – 0 0
Mitochondria 0.7 -1.7 -1.3 -2.2 -3.2 – -2.0 0.3 -0.8 -2.9 -8.5 -10.2 – -13.9 -14.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Nuclear Periphery -0.6 0 2.1 2.6 0.9 – 3.0 2.9 3.6 0 -1.4 -2.6 – 0 0
Nucleolus -0.8 0.1 2.3 3.3 4.1 – 1.8 1.9 1.4 0 -2.2 -1.5 – 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – 0 0
Vacuole -1.4 1.3 -0.6 -1.0 -0.4 – -0.5 -0.8 -1.9 2.8 6.8 6.3 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.025 4.0261 4.163 4.2545 3.4034 4.2535 7.0589 7.1622 7.1725 6.7677 6.8518 7.1286 4.2254 4.5527 3.9131 4.0669 3.3267 3.9886
Actin 0.01 0.0039 0.0035 0.0042 0.0047 0.0028 0.0035 0.0048 0.0039 0.0084 0.0003 0.0009 0.0168 0.0016 0.0108 0.0023 0.0004 0.0004
Bud 0.0002 0.0002 0.0004 0.0027 0.0008 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0
Bud Neck 0.0001 0.0003 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0 0 0.0004 0.0001 0.0002 0 0 0.0001
Bud Periphery 0.0004 0.0004 0.0009 0.0195 0.002 0.0021 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0004 0.0002 0.0003 0.0001 0.0003 0.0001
Bud Site 0.0006 0.0004 0.0011 0.0002 0.0007 0.0011 0.0002 0.0169 0.0002 0.0001 0 0.0001 0.001 0.0007 0.0004 0.0001 0 0.0001
Cell Periphery 0.0002 0.0001 0.0018 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0003 0.0001 0.0003 0 0.0002 0.0001 0.0001 0.0001 0.0002 0 0 0 0.0002 0.0001 0.0012 0 0 0
Cytoplasmic Foci 0.0074 0.0061 0.0062 0.0017 0.0096 0.0021 0.0027 0.0034 0.0017 0.0016 0.0004 0.0008 0.0102 0.007 0.0034 0.0024 0.0001 0.0019
Eisosomes 0.0004 0.0001 0.0006 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0 0 0 0.0004 0.0002 0.0005 0 0.0005 0.0001
Endosome 0.015 0.0145 0.0063 0.0062 0.0261 0.0049 0.0083 0.0081 0.0068 0.0019 0.0022 0.0022 0.019 0.0156 0.0114 0.0027 0.0018 0.0085
Golgi 0.0287 0.0484 0.0081 0.0042 0.066 0.0195 0.0155 0.0208 0.0116 0.0094 0.0031 0.0081 0.0292 0.0391 0.0161 0.0088 0.0027 0.0207
Lipid Particles 0.023 0.0147 0.0089 0.0011 0.0037 0.0085 0.0151 0.0213 0.0013 0.0015 0.0035 0.0009 0.0218 0.0121 0.0095 0.0255 0.0007 0.0069
Mitochondria 0.8884 0.8813 0.9415 0.9293 0.8606 0.9467 0.9345 0.9063 0.9638 0.9439 0.9767 0.9799 0.8703 0.8972 0.9323 0.949 0.9911 0.9495
None 0.0004 0 0.0007 0.0001 0.0001 0 0.0001 0 0.0004 0 0 0 0.0002 0 0.0004 0 0 0
Nuclear Periphery 0.0005 0 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0004 0 0 0 0.0008 0.0001 0.0009 0 0.0001 0.0001
Nucleolus 0.0002 0.0044 0.0002 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0.0004 0.0001 0.0001 0.0001 0 0.0001
Nucleus 0.0001 0.0003 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0 0.0003 0 0 0.0001
Peroxisomes 0.0173 0.021 0.0104 0.0247 0.0148 0.0056 0.0161 0.0159 0.0065 0.0323 0.0079 0.0055 0.02 0.0211 0.0072 0.0078 0.0007 0.005
Punctate Nuclear 0.0006 0.0001 0.0027 0 0 0 0.0001 0 0.0002 0 0 0 0.0003 0 0.001 0 0 0
Vacuole 0.0015 0.0016 0.0029 0.0013 0.003 0.0011 0.0009 0.0004 0.0008 0.0001 0.0009 0.0003 0.0022 0.0012 0.0011 0.0002 0.0002 0.002
Vacuole Periphery 0.0042 0.002 0.0026 0.0043 0.0068 0.0035 0.0019 0.0009 0.0013 0.0003 0.0044 0.001 0.0057 0.0032 0.0022 0.0005 0.0011 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.07 11.7847 12.2426 16.6403 15.3829 9.2229 23.2351 26.1369 21.6193 22.4978
Translational Efficiency 0.9836 1.5035 1.3014 0.8956 1.169 2.8433 0.9537 0.8507 1.2032 1.3245

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mba1

Mba1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mba1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available