Standard name | |
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Human Ortholog | |
Description | Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 17 | 26 | 82 | 37 | 57 | 82 | 174 | – | 160 | 186 | 269 | 85 | 199 | 302 | 50 | 78 | 32 | 1 | 2 | 0 | 2 | 4 | 5 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 3 | – | 0 | 2 | 0 | 1 | 3 | 2 | 11 | 25 | 17 | 0 | 0 | 0 | 0 | 2 | 2 |
Endosome | 6 | 0 | 17 | 5 | 9 | 2 | 2 | 2 | – | 0 | 0 | 2 | 4 | 4 | 1 | 6 | 9 | 6 | 10 | 8 | 12 | 9 | 18 | 25 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 2 |
Mitochondria | 14 | 33 | 89 | 14 | 27 | 40 | 43 | 25 | – | 46 | 45 | 53 | 0 | 0 | 0 | 26 | 26 | 13 | 2 | 4 | 6 | 4 | 7 | 11 |
Nucleus | 39 | 28 | 58 | 45 | 46 | 102 | 155 | 210 | – | 66 | 32 | 55 | 15 | 13 | 2 | 116 | 175 | 112 | 4 | 1 | 2 | 2 | 2 | 1 |
Nuclear Periphery | 3 | 2 | 1 | 2 | 3 | 3 | 5 | 12 | – | 1 | 3 | 2 | 0 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 2 | 1 | 3 | 5 |
Nucleolus | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 2 | – | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Vac/Vac Membrane | 82 | 152 | 278 | 249 | 269 | 270 | 395 | 587 | – | 295 | 238 | 340 | 116 | 117 | 50 | 111 | 167 | 163 | 129 | 171 | 375 | 248 | 380 | 330 |
Unique Cell Count | 123 | 189 | 375 | 312 | 329 | 374 | 548 | 794 | 459 | 385 | 570 | 194 | 295 | 333 | 248 | 406 | 274 | 157 | 195 | 408 | 276 | 432 | 398 | |
Labelled Cell Count | 154 | 235 | 470 | 403 | 391 | 477 | 683 | 1015 | 572 | 509 | 724 | 223 | 337 | 357 | 324 | 487 | 345 | 157 | 195 | 408 | 276 | 432 | 398 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.8 | 5.2 | 5.9 | 6.9 | 6.7 | 6.5 | 6.7 | – | 5.9 | 5.9 | 6.1 | 6.2 | 5.9 | 5.9 | 5.6 | 6.7 | 8.1 | 6.9 | 7.1 | 7.2 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.2 | 1.4 | 1.6 | 2.1 | 1.9 | 1.7 | – | 1.7 | 1.7 | 1.7 | 0.9 | 1.0 | 1.2 | 1.5 | 2.0 | 2.3 | 2.1 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.4 | 0.35 | 0.32 | 0.35 | – | 0.18 | 0.18 | 0.21 | 0.24 | 0.17 | 0.17 | 0.11 | 0.36 | 0.63 | 0.4 | 0.43 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.9 | 2.0 | 3.6 | 3.7 | 6.3 | – | 9.6 | 12.7 | 13.1 | 10.5 | 16.5 | 22.3 | 4.9 | 5.0 | 2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.9 | 4.9 |
Endosome | -2.2 | -1.3 | -3.5 | -4.4 | -5.4 | – | -4.6 | -4.2 | -4.5 | -1.5 | -2.3 | -3.6 | -1.4 | -1.8 | -1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -7.0 | -5.5 | -4.7 | -6.8 | -11.1 | – | -5.3 | -4.4 | -6.1 | -7.4 | -9.0 | -9.5 | -4.2 | -6.8 | -6.6 |
Nucleus | -0.4 | -0.6 | 3.9 | 4.5 | 4.2 | – | -0.4 | -3.1 | -2.7 | -2.6 | -4.6 | -7.1 | 8.5 | 8.4 | 7.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.8 | 2.4 | -0.6 | -0.7 | -0.1 | – | -3.1 | -3.6 | -4.6 | -3.5 | -9.0 | -15.7 | -7.4 | -9.3 | -3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0121 | 3.9476 | 3.1984 | 3.132 | 2.7937 | 3.4042 | 4.2604 | 6.2775 | 5.1629 | 5.0256 | 4.7281 | 5.6502 | 5.0015 | 7.4848 | 6.1941 | 6.305 | 5.3187 | 6.0723 |
Actin | 0.0256 | 0.0003 | 0 | 0 | 0 | 0.0024 | 0.0099 | 0 | 0.0044 | 0 | 0.0195 | 0.0032 | 0.0037 | 0 | 0.002 | 0.0248 | 0 | 0 |
Bud | 0.0006 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0.0004 |
Bud Neck | 0.0035 | 0.0009 | 0.0009 | 0.0005 | 0.0008 | 0.001 | 0.0007 | 0.0015 | 0.0039 | 0.0007 | 0.0011 | 0.0016 | 0.0025 | 0.0013 | 0.0029 | 0.0009 | 0.0011 | 0.0013 |
Bud Periphery | 0.0017 | 0.0007 | 0.001 | 0.0004 | 0.0008 | 0.0011 | 0.0007 | 0.0007 | 0.0016 | 0.0005 | 0.0008 | 0.0011 | 0.0016 | 0.0008 | 0.0013 | 0.0003 | 0.0008 | 0.0014 |
Bud Site | 0.0044 | 0.0018 | 0.0006 | 0.0003 | 0.0012 | 0.0005 | 0.0007 | 0.0013 | 0.0047 | 0.0007 | 0.0009 | 0.0008 | 0.0035 | 0.0009 | 0.0056 | 0.0006 | 0.0008 | 0.001 |
Cell Periphery | 0.0035 | 0.0021 | 0.0028 | 0.0018 | 0.0033 | 0.0042 | 0.0025 | 0.0037 | 0.0029 | 0.001 | 0.0026 | 0.0039 | 0.003 | 0.0029 | 0.0031 | 0.0014 | 0.0018 | 0.0029 |
Cytoplasm | 0.0152 | 0.0173 | 0.0063 | 0.0009 | 0.0007 | 0.0021 | 0.0049 | 0.0125 | 0.0056 | 0.0067 | 0.0019 | 0.0017 | 0.0028 | 0.0043 | 0.003 | 0.0013 | 0.0049 | 0.0013 |
Cytoplasmic Foci | 0.0089 | 0.0057 | 0.0066 | 0.0024 | 0.0011 | 0.0049 | 0.0102 | 0.0041 | 0.0066 | 0.0045 | 0.0117 | 0.0028 | 0.0042 | 0.0046 | 0.0059 | 0.0071 | 0.006 | 0.0018 |
Eisosomes | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0102 | 0.0018 | 0.0023 | 0.0005 | 0.0024 | 0.0018 | 0.0045 | 0.0033 | 0.0034 | 0.0016 | 0.0016 | 0.0018 | 0.0021 | 0.0032 | 0.0028 | 0.0005 | 0.0021 | 0.0015 |
Endosome | 0.1456 | 0.0888 | 0.1986 | 0.1568 | 0.0886 | 0.1004 | 0.1735 | 0.1001 | 0.1441 | 0.1953 | 0.1653 | 0.1126 | 0.1456 | 0.1039 | 0.1431 | 0.1587 | 0.1847 | 0.1024 |
Golgi | 0.0073 | 0.0019 | 0.004 | 0.0038 | 0.0019 | 0.0055 | 0.0077 | 0.004 | 0.009 | 0.0081 | 0.0084 | 0.004 | 0.0071 | 0.0047 | 0.0116 | 0.008 | 0.0042 | 0.0047 |
Lipid Particles | 0.0261 | 0.0044 | 0.0066 | 0.0067 | 0.0138 | 0.021 | 0.0152 | 0.0076 | 0.0215 | 0.0077 | 0.0172 | 0.011 | 0.0249 | 0.01 | 0.0234 | 0.0059 | 0.0048 | 0.0172 |
Mitochondria | 0.0362 | 0.013 | 0.0086 | 0.0019 | 0.0012 | 0.0161 | 0.0177 | 0.0041 | 0.0338 | 0.0326 | 0.0049 | 0.0056 | 0.0304 | 0.0026 | 0.0267 | 0.0014 | 0.0022 | 0.0091 |
None | 0.0274 | 0.0026 | 0.0035 | 0.0001 | 0.0001 | 0.0006 | 0.0036 | 0.0065 | 0.0078 | 0.0067 | 0.001 | 0.0002 | 0.0014 | 0.0015 | 0.0005 | 0.0001 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.019 | 0.0064 | 0.0066 | 0.0011 | 0.0118 | 0.0168 | 0.006 | 0.0053 | 0.0124 | 0.0021 | 0.0027 | 0.0048 | 0.0056 | 0.0152 | 0.0106 | 0.0013 | 0.0053 | 0.0049 |
Nucleolus | 0.0158 | 0.0038 | 0.0085 | 0.0035 | 0.0059 | 0.0031 | 0.0101 | 0.0039 | 0.0077 | 0.0024 | 0.003 | 0.0105 | 0.0129 | 0.0087 | 0.0066 | 0.0017 | 0.0023 | 0.0078 |
Nucleus | 0.0338 | 0.0062 | 0.0042 | 0.0023 | 0.0044 | 0.005 | 0.0073 | 0.0046 | 0.0116 | 0.003 | 0.0023 | 0.0092 | 0.0082 | 0.0093 | 0.0146 | 0.0014 | 0.0099 | 0.0048 |
Peroxisomes | 0.0027 | 0.0003 | 0.0006 | 0.0004 | 0.0005 | 0.0015 | 0.003 | 0.0006 | 0.0033 | 0.0006 | 0.0064 | 0.0012 | 0.002 | 0.0004 | 0.0018 | 0.0008 | 0.0002 | 0.0005 |
Punctate Nuclear | 0.0097 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0027 | 0.0057 | 0.0001 | 0.0025 | 0.0001 | 0.0048 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 |
Vacuole | 0.4168 | 0.6571 | 0.5416 | 0.6535 | 0.704 | 0.6122 | 0.4864 | 0.6446 | 0.5002 | 0.5795 | 0.6074 | 0.6217 | 0.4982 | 0.621 | 0.5222 | 0.5885 | 0.6002 | 0.6053 |
Vacuole Periphery | 0.1853 | 0.1842 | 0.1961 | 0.163 | 0.157 | 0.1968 | 0.2295 | 0.1911 | 0.2122 | 0.1458 | 0.1363 | 0.2017 | 0.2388 | 0.2042 | 0.2114 | 0.1951 | 0.1679 | 0.2314 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.1589 | 47.3743 | 55.6165 | 67.26 | 61.5317 | 45.8509 | 62.02 | 66.1063 | 74.933 | 67.6196 |
Translational Efficiency | 1.0171 | 1.1003 | 0.8925 | 1.0151 | 0.81 | 1.5088 | 0.9639 | 0.9398 | 0.97 | 0.8688 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
79 | 1251 | 521 | 1453 | 2244 | 1556 | 2019 | 2159 | 2323 | 2807 | 2540 | 3612 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 965.66 | 780.77 | 842.07 | 892.55 | 778.30 | 770.37 | 878.00 | 891.74 | 784.67 | 775.00 | 870.63 | 892.07 |
Standard Deviation | 114.57 | 130.10 | 91.69 | 135.49 | 109.67 | 97.90 | 104.09 | 121.83 | 114.97 | 113.50 | 102.70 | 127.50 |
Intensity Change Log 2 | — | -0.306618 | -0.197575 | -0.113582 | — | -0.014775 | 0.173895 | 0.196297 | — | -0.169038 | -0.019900 | 0.032983 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000537 | 0.000400 | 0.000093 | 0.000692 | 0.000542 | 0.000043 | 0.000080 | 0.000331 | 0.000542 | 0.000202 | 0.000083 | 0.000476 |
Bud Neck | 0.008854 | 0.018103 | 0.028087 | 0.016332 | 0.007084 | 0.012896 | 0.027370 | 0.020651 | 0.007144 | 0.015216 | 0.027517 | 0.018913 |
Bud Site | 0.017017 | 0.014312 | 0.005644 | 0.029972 | 0.006925 | 0.007491 | 0.012301 | 0.026061 | 0.007268 | 0.010531 | 0.010936 | 0.027634 |
Cell Periphery | 0.001922 | 0.000228 | 0.000255 | 0.000092 | 0.001750 | 0.000397 | 0.000034 | 0.000088 | 0.001756 | 0.000322 | 0.000079 | 0.000089 |
Cytoplasm | 0.044996 | 0.108302* | 0.164154* | 0.101750* | 0.009935 | 0.021825 | 0.091240 | 0.107244* | 0.011127 | 0.060366 | 0.106196* | 0.105034* |
Cytoplasmic Foci | 0.026045 | 0.030790 | 0.042329 | 0.081572 | 0.005635 | 0.011759 | 0.057122 | 0.057424 | 0.006329 | 0.020241 | 0.054088 | 0.067138 |
Eisosomes | 0.000033 | 0.000022 | 0.000127 | 0.000023 | 0.000010 | 0.000018 | 0.000017 | 0.000018 | 0.000011 | 0.000020 | 0.000040 | 0.000020 |
Endoplasmic Reticulum | 0.003543 | 0.001373 | 0.002919 | 0.001210 | 0.003044 | 0.002001 | 0.001390 | 0.001106 | 0.003061 | 0.001721 | 0.001704 | 0.001148 |
Endosome | 0.109377 | 0.065546 | 0.071396 | 0.190479 | 0.036028 | 0.075281 | 0.237083* | 0.198794 | 0.038523 | 0.070942 | 0.203098* | 0.195449 |
Golgi | 0.007748 | 0.001098 | 0.000574 | 0.005241 | 0.001340 | 0.001202 | 0.001611 | 0.003409 | 0.001558 | 0.001156 | 0.001399 | 0.004146 |
Lipid Particles | 0.002869 | 0.001305 | 0.005806 | 0.004002 | 0.005115 | 0.001986 | 0.003090 | 0.003005 | 0.005038 | 0.001682 | 0.003647 | 0.003406 |
Mitochondria | 0.001879 | 0.000789 | 0.000667 | 0.002726 | 0.001345 | 0.006168 | 0.001351 | 0.002038 | 0.001363 | 0.003771 | 0.001211 | 0.002315 |
Mitotic Spindle | 0.001412 | 0.001885 | 0.001972 | 0.016454 | 0.000115 | 0.000268 | 0.005644 | 0.010260 | 0.000159 | 0.000989 | 0.004891 | 0.012751 |
None | 0.000148 | 0.002293 | 0.003084 | 0.000236 | 0.000194 | 0.000222 | 0.000199 | 0.000467 | 0.000192 | 0.001145 | 0.000791 | 0.000374 |
Nuclear Periphery | 0.001545 | 0.000327 | 0.003595 | 0.000605 | 0.000144 | 0.000391 | 0.001344 | 0.001142 | 0.000192 | 0.000363 | 0.001805 | 0.000926 |
Nuclear Periphery Foci | 0.000564 | 0.000349 | 0.002991 | 0.002140 | 0.000082 | 0.000118 | 0.002550 | 0.001232 | 0.000098 | 0.000221 | 0.002640 | 0.001597 |
Nucleolus | 0.004467 | 0.001869 | 0.010981 | 0.001123 | 0.000271 | 0.001168 | 0.004270 | 0.002366 | 0.000414 | 0.001481 | 0.005647 | 0.001866 |
Nucleus | 0.048174 | 0.020365 | 0.369665* | 0.006920 | 0.001226 | 0.006980 | 0.022348 | 0.013033 | 0.002823 | 0.012945 | 0.093589 | 0.010574 |
Peroxisomes | 0.004831 | 0.001496 | 0.004430 | 0.011804 | 0.002113 | 0.001256 | 0.005855 | 0.006681 | 0.002205 | 0.001363 | 0.005563 | 0.008742 |
Vacuole | 0.671211* | 0.708935* | 0.279181* | 0.516930* | 0.892496* | 0.815599* | 0.518652* | 0.533386* | 0.884971* | 0.768062* | 0.469533* | 0.526766* |
Vacuole Periphery | 0.042827 | 0.020213 | 0.002048 | 0.009697 | 0.024607 | 0.032931 | 0.006447 | 0.011267 | 0.025226 | 0.027263 | 0.005545 | 0.010635 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.31 | 1.47 | -0.34 | -0.65 | -3.38 | 2.00 | 1.83 | 0.90 | -2.73 | -2.33 | 1.19 | 1.84 | 0.40 | -1.40 | -4.21 |
Bud Neck | -2.70 | -4.65 | -2.52 | 0.57 | 3.59 | -4.89 | -13.98 | -12.23 | -6.07 | 4.29 | -7.45 | -14.91 | -13.04 | -3.94 | 6.22 |
Bud Site | 0.52 | 1.47 | -1.54 | -6.35 | -9.53 | -0.42 | -4.34 | -9.72 | -9.07 | -7.14 | -2.79 | -3.45 | -12.62 | -10.81 | -10.90 |
Cell Periphery | 0.96 | 0.98 | 1.04 | 3.25 | 2.39 | 8.92 | 11.48 | 11.11 | 10.36 | -2.80 | 9.12 | 10.82 | 10.72 | 9.25 | -1.00 |
Cytoplasm | -3.75 | -7.20 | -4.07 | -0.63 | 6.42 | -4.84 | -23.87 | -24.75 | -20.71 | -1.72 | -16.17 | -28.88 | -30.93 | -13.21 | 1.16 |
Cytoplasmic Foci | -0.72 | -1.82 | -7.09 | -13.61 | -9.86 | -4.69 | -25.97 | -25.62 | -21.04 | 0.06 | -11.12 | -27.21 | -33.43 | -23.43 | -5.23 |
Eisosomes | 1.70 | -4.76 | 1.71 | 0.00 | 6.16 | -4.59 | -5.54 | -5.27 | -0.01 | -0.04 | -5.63 | -8.00 | -6.05 | 0.14 | 5.36 |
Endoplasmic Reticulum | 3.01 | -0.11 | 3.21 | 0.93 | 8.23 | 3.87 | 6.58 | 8.07 | 5.29 | 2.91 | 5.66 | 4.95 | 8.25 | 4.70 | 6.32 |
Endosome | 1.88 | 2.03 | -2.48 | -16.18 | -13.26 | -9.89 | -29.35* | -27.77 | -17.86 | 3.50 | -10.18 | -27.04 | -32.56 | -24.19 | 0.17 |
Golgi | 1.27 | 1.39 | 0.63 | -3.94 | -4.92 | 0.39 | -0.03 | -3.32 | -3.42 | -3.34 | 1.19 | 1.20 | -4.01 | -5.23 | -5.35 |
Lipid Particles | 1.59 | -1.74 | -1.20 | -7.20 | 1.05 | 2.35 | 1.29 | 1.38 | -2.87 | 0.29 | 2.67 | 1.01 | 1.03 | -6.49 | 0.05 |
Mitochondria | 0.83 | 0.88 | -0.31 | -2.29 | -2.42 | -3.47 | 0.09 | -1.56 | 3.07 | -2.18 | -2.91 | 0.46 | -1.70 | 2.01 | -2.71 |
Mitotic Spindle | -0.32 | -1.96 | -6.46 | -6.27 | -5.40 | -1.28 | -6.91 | -8.74 | -8.63 | -3.45 | -2.12 | -7.60 | -11.25 | -10.24 | -5.67 |
None | -2.44 | -4.24 | -0.82 | 2.35 | 4.12 | -0.30 | -0.89 | -1.97 | -1.85 | -1.71 | -2.41 | -4.04 | -1.82 | 1.91 | 2.71 |
Nuclear Periphery | 2.85 | -5.09 | 1.74 | -3.33 | 8.36 | -2.42 | -11.96 | -8.19 | -6.31 | 2.29 | -2.06 | -14.77 | -8.66 | -7.17 | 7.34 |
Nuclear Periphery Foci | 1.74 | -5.25 | -4.28 | -5.05 | 3.46 | -1.68 | -6.39 | -7.59 | -7.42 | 2.94 | -4.84 | -8.39 | -9.16 | -8.44 | 3.89 |
Nucleolus | 1.09 | -2.46 | 1.28 | 1.42 | 6.02 | -2.62 | -10.39 | -8.52 | -3.92 | 3.74 | -4.42 | -11.49 | -8.32 | -2.84 | 7.50 |
Nucleus | 1.89 | -13.59 | 2.69 | 5.81 | 23.82 | -5.80 | -17.57 | -14.70 | -6.26 | 6.99 | -8.87 | -22.39 | -11.46 | 0.27 | 19.70 |
Peroxisomes | 1.58 | 0.53 | -2.42 | -8.17 | -5.07 | 1.34 | -4.80 | -5.47 | -8.45 | -1.47 | 1.41 | -4.37 | -7.57 | -11.68 | -4.59 |
Vacuole | -1.04 | 9.30 | 3.79 | 15.78 | -14.98 | 10.42 | 49.08* | 46.26* | 32.14* | -1.36 | 17.78 | 56.02* | 53.24* | 33.34 | -6.81 |
Vacuole Periphery | 1.82 | 3.33 | 2.78 | 7.30 | -7.63 | -4.09 | 16.34 | 11.32 | 12.86 | -7.18 | -1.14 | 17.11 | 12.45 | 14.60 | -8.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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