Standard name
Human Ortholog
Description Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.38 0.52 0.42 0.41 0.31 0.3 0.19 0.22 0.3 0.23 0.29 0.37 0.49 0.42 0.35 0.27 0.27 0.29 0.25 0.21 0.26 0.12 0.13 0.13
Bud 0.19 0.22 0.21 0.21 0.22 0.21 0.18 0.23 0.17 0.18 0.18 0.21 0.2 0.19 0.07 0.08 0.05 0.1 0.31 0.31 0.27 0.49 0.51 0.41
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.26 0.21 0.27 0.2 0.22
Bud Site 0.25 0.15 0.26 0.16 0.1 0.14 0.1 0.05 0.05 0.05 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.11 0.13 0.12 0.1 0.07 0.11 0.13 0.08 0.17 0.15 0.15 0.25 0.37 0.43 0.59 0.12 0.19 0.17 0.06 0.07 0.09 0 0 0
Cytoplasm 0.21 0.24 0.19 0.21 0.33 0.3 0.35 0.33 0.3 0.33 0.33 0.23 0.31 0.29 0.25 0.41 0.31 0.32 0.06 0 0.06 0.06 0.06 0.1
Endoplasmic Reticulum 0 0.11 0.07 0 0.05 0.09 0.07 0.07 0.07 0.08 0.09 0.07 0 0 0.06 0 0.05 0 0 0 0 0 0 0
Endosome 0.09 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.09 0.08 0.05 0.21 0.2 0.19 0.33 0.31 0.35 0.37 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.09 0.07 0.15 0.16 0.11 0.09 0.07 0.07 0.06 0.05 0.06 0 0 0.06 0 0 0 0.11 0 0 0 0 0 0
SpindlePole 0.07 0.06 0.12 0.05 0.09 0 0.07 0.06 0 0 0 0 0.06 0 0 0.2 0.27 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 131 134 143 104 86 123 92 119 128 111 79 79 188 207 163 78 41 42 100 70 114 44 76 72
Bud 68 57 71 53 61 88 85 123 73 83 48 46 78 95 34 23 8 14 121 105 118 184 294 228
Bud Neck 1 3 2 2 1 1 1 2 4 0 1 0 0 0 2 1 4 0 95 86 92 101 115 125
Bud Site 86 40 87 41 29 57 45 27 23 25 14 7 15 13 0 10 4 3 – – – – – –
Cell Periphery 39 34 40 25 19 45 60 45 73 69 40 54 140 213 277 36 29 25 24 24 37 4 11 10
Cytoplasm 72 62 66 55 93 122 165 177 126 155 88 50 118 143 117 121 47 46 24 13 23 21 37 57
Endoplasmic Reticulum 7 28 24 11 15 36 31 37 30 37 23 16 17 11 26 8 8 3 6 8 8 2 10 10
Endosome 32 3 10 9 19 15 15 18 4 9 3 2 1 1 0 6 1 8 3 5 7 3 10 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 2 6 6 1 2 3 2 0 1
Mitochondria 42 3 29 21 13 85 93 101 142 147 95 80 3 0 0 18 7 6 4 6 10 2 6 9
Nucleus 9 9 4 4 6 3 6 13 5 4 3 1 1 0 0 10 5 5 0 0 0 1 0 0
Nuclear Periphery 0 1 2 0 0 1 0 0 1 1 2 0 0 0 1 0 0 0 0 0 0 2 2 4
Nucleolus 17 3 7 6 7 7 11 24 8 11 2 3 1 0 1 3 2 2 0 0 0 0 1 0
Peroxisomes 31 17 50 42 31 39 34 35 25 22 15 7 17 28 7 7 5 16 0 0 3 0 0 1
SpindlePole 26 15 42 14 25 17 31 32 15 20 6 1 23 17 6 58 41 27 3 2 4 2 1 5
Vac/Vac Membrane 5 5 7 5 11 4 19 25 7 16 3 2 2 2 2 22 9 9 1 8 3 2 4 10
Unique Cell Count 349 260 340 256 281 413 473 530 424 473 268 216 382 493 469 293 150 145 393 338 434 380 582 562
Labelled Cell Count 566 415 584 392 416 643 688 778 664 710 422 348 605 730 636 403 217 212 393 338 434 380 582 562


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 8.8 7.5 7.6 7.7 6.7 6.2 6.4 6.3 6.1 5.9 6.2 9.2 9.2 9.1 9.6 12.3 11.3 7.6 7.7 8.2
Std Deviation (1e-4) 1.4 1.6 1.3 1.5 2.7 1.5 1.6 1.8 1.2 1.6 1.4 1.2 1.8 1.7 1.6 2.3 2.8 2.8 1.2 1.5 1.9
Intensity Change (Log2) – – – 0.01 0.03 -0.18 -0.29 -0.25 -0.27 -0.32 -0.35 -0.29 0.29 0.29 0.27 0.34 0.71 0.58 0.01 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.4 -2.9 -3.5 -7.0 -6.2 -3.4 -5.6 -3.2 -1.3 1.9 0 -2.1 -4.1 -3.1 -2.7
Bud -0.1 0.3 0.1 -1.0 0.8 -1.3 -1.2 -0.9 0.1 -0.2 -0.6 -5.7 -4.6 -4.3 -3.0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -2.8 -4.9 -4.1 -6.1 -8.7 -7.9 -8.3 -6.7 -6.9 -8.3 -10.0 -11.6 -7.7 -6.0 -6.1
Cell Periphery -0.8 -2.1 -0.4 0.4 -1.6 2.1 1.2 1.1 4.1 7.7 9.7 13.6 0.2 2.2 1.6
Cytoplasm 0.6 3.9 3.2 4.8 4.5 3.3 4.2 3.8 1.1 3.5 3.1 1.9 6.0 2.9 2.9
Endoplasmic Reticulum -1.4 -0.9 0.8 -0.3 0 0 0.4 0.7 0.2 -1.5 -3.4 -0.9 -2.5 -0.7 -2.2
Endosome 0.4 2.2 0.5 0.2 0.4 -2.0 -1.0 -1.5 0 -2.9 0 0 -0.7 0 1.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.1 -1.9 4.6 4.4 4.2 8.2 7.7 8.2 8.3 -5.0 -6.6 -6.4 -1.1 -1.5 -1.7
Nucleus 0 0 0 0 1.3 0 0 0 0 0 0 0 1.9 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.4 -0.4 0.3 1.9 -0.2 0.3 0 0 0 0 0 0 0 0
Peroxisomes 0.6 -1.4 -2.2 -3.5 -3.9 -4.1 -5.0 -3.6 -4.3 -4.7 -4.4 -7.2 -5.4 -3.7 -1.1
SpindlePole -2.9 -1.4 -4.2 -2.9 -3.3 -4.6 -4.3 -4.6 -5.1 -3.0 -4.9 -6.6 2.6 4.1 1.8
Vacuole -0.1 1.4 0 1.6 2.0 -0.4 1.1 0 0 0 0 0 3.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.124 5.2442 5.6935 4.958 5.1346 5.6514 6.1913 6.9202 6.5212 6.2528 6.0131 6.5253 7.9505 7.6597 7.574 7.4978 7.322 8.155
Actin 0.1287 0.0154 0.0343 0.0941 0.0762 0.099 0.1847 0.0462 0.0998 0.1393 0.1385 0.1217 0.0954 0.0508 0.061 0.0404 0.0668 0.067
Bud 0.0151 0.0245 0.2596 0.0867 0.0641 0.0089 0.0044 0.0501 0.1833 0.13 0.0421 0.0075 0.0235 0.041 0.1738 0.1099 0.0827 0.0117
Bud Neck 0.1252 0.0104 0.0055 0.0025 0.1456 0.6062 0.0634 0.0083 0.0441 0.0003 0.0023 0.5484 0.0896 0.0097 0.0181 0.0017 0.0541 0.66
Bud Periphery 0.0736 0.0507 0.3844 0.439 0.4423 0.0473 0.0073 0.0336 0.338 0.6835 0.3145 0.0596 0.0765 0.0471 0.4343 0.6554 0.3936 0.0548
Bud Site 0.2432 0.3034 0.108 0.0091 0.0572 0.0174 0.3603 0.5522 0.142 0.0111 0.0099 0.0117 0.2955 0.5288 0.1678 0.0058 0.0829 0.0218
Cell Periphery 0.0646 0.0124 0.0101 0.0235 0.0604 0.036 0.0448 0.0124 0.0175 0.0155 0.0067 0.0323 0.1129 0.0278 0.0214 0.0163 0.0704 0.0318
Cytoplasm 0.1689 0.4951 0.1124 0.3201 0.0773 0.1306 0.0826 0.2626 0.1098 0.0159 0.158 0.17 0.1519 0.2442 0.0543 0.1613 0.0826 0.0976
Cytoplasmic Foci 0.1134 0.0105 0.0157 0.0014 0.002 0.0233 0.0772 0.0099 0.0075 0.0002 0.0832 0.0131 0.0236 0.0091 0.0092 0.0004 0.0251 0.0262
Eisosomes 0.0015 0.0005 0.0003 0.0008 0.0004 0.0004 0.0028 0.0007 0.0004 0.0002 0.0003 0.0009 0.0022 0.0006 0.0002 0.0001 0.0005 0.0003
Endoplasmic Reticulum 0.0043 0.0056 0.0034 0.0071 0.0009 0.0021 0.002 0.0015 0.0021 0.0006 0.0037 0.0027 0.0084 0.0015 0.0049 0.0018 0.004 0.0006
Endosome 0.0102 0.005 0.0061 0.0015 0.0015 0.0012 0.0349 0.0008 0.003 0.0003 0.0857 0.0112 0.0286 0.0036 0.0121 0.0003 0.037 0.0062
Golgi 0.0092 0.0028 0.0037 0.001 0.0029 0.0021 0.0315 0.0016 0.0071 0.0005 0.1058 0.0061 0.0131 0.0013 0.003 0.0003 0.0325 0.0056
Lipid Particles 0.0109 0.0015 0.0031 0.0005 0.0003 0.009 0.0197 0.0004 0.0118 0 0.0056 0.0013 0.0095 0.0005 0.0046 0.0001 0.02 0.0033
Mitochondria 0.0013 0.0008 0.002 0.0005 0.0626 0.0004 0.0128 0.0002 0.0213 0.0004 0.0207 0.002 0.01 0.0003 0.0071 0.0002 0.0089 0.0013
None 0.0023 0.0473 0.026 0.0036 0.0007 0.0045 0.003 0.0153 0.0023 0.0004 0.0018 0.0026 0.0292 0.0267 0.0126 0.0014 0.005 0.0029
Nuclear Periphery 0.0008 0.0014 0.0018 0.0009 0.0002 0.0002 0.0013 0.0004 0.0004 0.0002 0.0006 0.0012 0.0044 0.0004 0.0026 0.0006 0.004 0.0001
Nucleolus 0.0005 0.0003 0.0008 0.0001 0.0004 0.0002 0.0006 0.0001 0.0002 0 0.0001 0.0001 0.0005 0.0003 0.0003 0.0001 0.0031 0.0001
Nucleus 0.0007 0.002 0.0031 0.0008 0.0005 0.0004 0.0008 0.0006 0.0006 0.0002 0.0004 0.0005 0.0013 0.001 0.0012 0.0006 0.008 0.0004
Peroxisomes 0.0068 0.0002 0.0112 0.0001 0.0018 0.0081 0.0597 0.0014 0.0063 0 0.0169 0.0053 0.0146 0.0012 0.0079 0 0.0044 0.0064
Punctate Nuclear 0.0129 0.0006 0.0037 0.0002 0.0002 0.0009 0.0043 0.0003 0.0003 0 0.0006 0.0005 0.0041 0.0008 0.0006 0.0001 0.0059 0.0005
Vacuole 0.0053 0.0089 0.0043 0.0061 0.002 0.0017 0.0013 0.0012 0.0019 0.001 0.0018 0.0009 0.0036 0.0031 0.0024 0.0029 0.0074 0.0011
Vacuole Periphery 0.0005 0.0007 0.0005 0.0004 0.0005 0.0001 0.0005 0.0001 0.0003 0.0001 0.001 0.0002 0.0013 0.0002 0.0004 0.0002 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.3256 15.4866 15.9116 26.4589 14.6862 17.9803 18.3686 22.7627 25.2492 21.7859
Translational Efficiency 0.9501 1.0638 1.4267 1.0312 0.9513 0.8654 1.0063 0.8726 0.9138 0.8412

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
Localization
Cell Percentages cytoplasm (16%), bud neck (14%), mixed (32%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bem1

Bem1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available