Standard name | |
---|---|
Human Ortholog | |
Description | Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 0 | 3 | 15 | 11 | 9 | 13 | 18 | 23 | 29 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 224 | 289 | 343 | 316 | 529 | 639 | 788 | 682 | 700 | 792 | 679 | 895 | 286 | 235 | 331 | 83 | 72 | 47 | 8 | 41 | 23 | 13 | 5 | 8 |
Endoplasmic Reticulum | 4 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 2 | 1 | 8 | 16 | 13 | 2 | 3 | 3 | 2 | 4 | 3 |
Endosome | 43 | 13 | 16 | 4 | 25 | 4 | 6 | 11 | 4 | 17 | 4 | 7 | 7 | 0 | 3 | 4 | 11 | 8 | 45 | 26 | 25 | 12 | 5 | 20 |
Golgi | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 5 | 7 | 3 | 1 | 1 | 0 | 0 |
Mitochondria | 30 | 10 | 47 | 26 | 35 | 92 | 133 | 163 | 160 | 122 | 199 | 227 | 0 | 0 | 0 | 7 | 4 | 4 | 20 | 6 | 16 | 12 | 8 | 13 |
Nucleus | 45 | 4 | 6 | 4 | 6 | 15 | 21 | 34 | 18 | 16 | 10 | 15 | 2 | 1 | 0 | 100 | 82 | 56 | 1 | 1 | 1 | 1 | 1 | 2 |
Nuclear Periphery | 0 | 2 | 1 | 3 | 7 | 1 | 12 | 15 | 6 | 6 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 2 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 7 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 2 | 1 | 1 | 3 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | 1 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 433 | 232 | 207 | 127 | 229 | 227 | 189 | 172 | 201 | 275 | 195 | 203 | 61 | 13 | 14 | 173 | 225 | 152 | 582 | 418 | 447 | 272 | 216 | 309 |
Unique Cell Count | 615 | 475 | 497 | 396 | 700 | 821 | 1001 | 941 | 888 | 1059 | 882 | 1139 | 328 | 246 | 343 | 282 | 327 | 227 | 683 | 514 | 531 | 323 | 253 | 373 |
Labelled Cell Count | 781 | 552 | 623 | 481 | 842 | 995 | 1164 | 1095 | 1107 | 1250 | 1121 | 1387 | 357 | 252 | 355 | 378 | 418 | 287 | 683 | 514 | 531 | 323 | 253 | 373 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 5.3 | 4.3 | 4.4 | 4.9 | 4.7 | 4.1 | 4.2 | 4.1 | 4.2 | 4.1 | 4.2 | 6.0 | 5.6 | 5.6 | 7.7 | 7.6 | 7.8 | 6.8 | 7.1 | 7.0 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.8 | 1.3 | 1.3 | 1.3 | 1.0 | 1.1 | 1.2 | 1.0 | 0.9 | 1.0 | 0.9 | 1.2 | 1.2 | 1.7 | 1.8 | 1.9 | 1.3 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.18 | 0.14 | -0.05 | -0.03 | -0.05 | -0.03 | -0.08 | -0.04 | 0.48 | 0.39 | 0.38 | 0.85 | 0.83 | 0.87 | 0.66 | 0.73 | 0.71 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 2.5 | 3.6 | 4.1 | 1.4 | 4.1 | 2.4 | 3.2 | 4.1 | 6.0 | 8.2 | 9.8 | -10.7 | -13.2 | -12.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | -2.8 | -2.2 | -1.5 | 0.1 | 0.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.7 | -5.0 | -5.9 | -3.7 | -4.8 | -3.8 |
Nucleus | 0 | 0 | 0.9 | 1.2 | 2.6 | 1.1 | 0.5 | -0.1 | 0.2 | 0 | 0 | 0 | 13.4 | 10.9 | 10.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | -3.2 | -5.2 | -9.4 | -9.6 | -7.4 | -6.2 | -7.7 | -10.2 | -6.9 | -10.2 | -12.2 | 5.3 | 7.6 | 6.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2434 | 2.3355 | 1.9008 | 1.7956 | 1.4193 | 2.1722 | 3.638 | 5.4628 | 4.5018 | 4.5626 | 4.1912 | 4.95 | 1.7904 | 3.3621 | 2.7801 | 3.0979 | 3.1888 | 2.8528 |
Actin | 0.0098 | 0.0001 | 0.0027 | 0.0064 | 0 | 0 | 0.0044 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Bud | 0.0018 | 0.0029 | 0.0053 | 0.0026 | 0.0009 | 0.0008 | 0.0005 | 0.0004 | 0.0009 | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0003 |
Bud Neck | 0.0016 | 0.0013 | 0.0018 | 0.0013 | 0.0017 | 0.0028 | 0.0013 | 0.0014 | 0.001 | 0.0028 | 0.0018 | 0.0016 | 0.0005 | 0.0025 | 0.003 | 0.001 | 0.0023 | 0.0015 |
Bud Periphery | 0.0017 | 0.0034 | 0.0044 | 0.0034 | 0.005 | 0.002 | 0.0012 | 0.001 | 0.0021 | 0.001 | 0.0017 | 0.0015 | 0.0006 | 0.0007 | 0.0009 | 0.0005 | 0.0013 | 0.0009 |
Bud Site | 0.0047 | 0.0042 | 0.0098 | 0.0143 | 0.0011 | 0.0016 | 0.0012 | 0.0011 | 0.0019 | 0.002 | 0.0031 | 0.0008 | 0.0004 | 0.0011 | 0.0012 | 0.0019 | 0.0019 | 0.0006 |
Cell Periphery | 0.0013 | 0.0015 | 0.001 | 0.0008 | 0.0032 | 0.0017 | 0.0015 | 0.0015 | 0.0015 | 0.0023 | 0.0015 | 0.0015 | 0.001 | 0.0021 | 0.0015 | 0.0005 | 0.0015 | 0.0022 |
Cytoplasm | 0.033 | 0.0266 | 0.0211 | 0.008 | 0.0037 | 0.0138 | 0.0206 | 0.0118 | 0.0128 | 0.0038 | 0.0072 | 0.0093 | 0.0132 | 0.0269 | 0.0107 | 0.0064 | 0.0049 | 0.009 |
Cytoplasmic Foci | 0.0347 | 0.0145 | 0.035 | 0.0301 | 0.0048 | 0.0132 | 0.016 | 0.0058 | 0.0122 | 0.0085 | 0.0049 | 0.0068 | 0.009 | 0.0111 | 0.0101 | 0.0065 | 0.0025 | 0.0053 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0063 | 0.0053 | 0.0042 | 0.0013 | 0.0012 | 0.0033 | 0.0064 | 0.0039 | 0.0048 | 0.002 | 0.0026 | 0.0042 | 0.0097 | 0.0085 | 0.0097 | 0.0012 | 0.0016 | 0.0043 |
Endosome | 0.3971 | 0.2619 | 0.3859 | 0.2734 | 0.2318 | 0.1841 | 0.3499 | 0.206 | 0.2777 | 0.2903 | 0.1574 | 0.2316 | 0.3305 | 0.1859 | 0.249 | 0.2215 | 0.1103 | 0.168 |
Golgi | 0.0229 | 0.0048 | 0.02 | 0.0073 | 0.0029 | 0.0047 | 0.0096 | 0.0054 | 0.0088 | 0.0063 | 0.0026 | 0.0069 | 0.0067 | 0.0028 | 0.0073 | 0.0018 | 0.0007 | 0.0031 |
Lipid Particles | 0.0239 | 0.0052 | 0.019 | 0.0056 | 0.002 | 0.006 | 0.011 | 0.0033 | 0.0039 | 0.007 | 0.0023 | 0.0055 | 0.0062 | 0.0047 | 0.0057 | 0.0012 | 0.001 | 0.0089 |
Mitochondria | 0.0285 | 0.006 | 0.0309 | 0.0144 | 0.0772 | 0.0077 | 0.0146 | 0.0043 | 0.0183 | 0.0027 | 0.0022 | 0.0079 | 0.0119 | 0.0021 | 0.0102 | 0.0003 | 0.0007 | 0.0089 |
None | 0.0204 | 0.0011 | 0.0052 | 0.0014 | 0.001 | 0.0008 | 0.0121 | 0.0104 | 0.0031 | 0.0004 | 0.0005 | 0.0026 | 0.019 | 0.0091 | 0.0057 | 0.0002 | 0.0003 | 0.0019 |
Nuclear Periphery | 0.0103 | 0.0106 | 0.0073 | 0.0026 | 0.0027 | 0.0073 | 0.0105 | 0.0041 | 0.0063 | 0.0027 | 0.0039 | 0.006 | 0.0187 | 0.0109 | 0.0114 | 0.003 | 0.0029 | 0.0065 |
Nucleolus | 0.0037 | 0.0029 | 0.0067 | 0.0052 | 0.0021 | 0.0028 | 0.0011 | 0.0011 | 0.0016 | 0.0019 | 0.0014 | 0.0015 | 0.0012 | 0.0018 | 0.0022 | 0.0025 | 0.0026 | 0.0019 |
Nucleus | 0.0076 | 0.0082 | 0.0076 | 0.0069 | 0.0039 | 0.006 | 0.0031 | 0.003 | 0.005 | 0.0019 | 0.0035 | 0.0045 | 0.006 | 0.0045 | 0.004 | 0.0025 | 0.0041 | 0.0053 |
Peroxisomes | 0.0078 | 0.0006 | 0.0045 | 0.0321 | 0.0006 | 0.0006 | 0.0015 | 0.0003 | 0.0006 | 0.001 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0003 |
Punctate Nuclear | 0.0047 | 0.0016 | 0.0018 | 0.0006 | 0.0001 | 0.0008 | 0.0008 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.309 | 0.5548 | 0.3402 | 0.5248 | 0.5806 | 0.6373 | 0.4373 | 0.6415 | 0.5244 | 0.5913 | 0.6899 | 0.5854 | 0.4462 | 0.6205 | 0.542 | 0.6287 | 0.7739 | 0.6438 |
Vacuole Periphery | 0.069 | 0.0825 | 0.0855 | 0.0575 | 0.0735 | 0.1025 | 0.0951 | 0.0936 | 0.1124 | 0.0716 | 0.1126 | 0.1207 | 0.1176 | 0.104 | 0.1241 | 0.1195 | 0.0868 | 0.127 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 80.3714 | 82.8069 | 59.639 | 72.0027 | 77.7123 | 94.3955 | 83.726 | 73.4068 | 79.0936 | 88.0179 |
Translational Efficiency | 1.3907 | 1.0955 | 1.2275 | 0.9448 | 1.0938 | 0.9855 | 0.9755 | 1.1766 | 0.9116 | 1.0094 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2827 | 1198 | 205 | 1266 | 1792 | 1402 | 2911 | 1454 | 4619 | 2600 | 3116 | 2720 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 729.40 | 759.88 | 865.41 | 910.98 | 696.60 | 756.14 | 914.29 | 896.88 | 716.67 | 757.86 | 911.07 | 903.44 |
Standard Deviation | 84.54 | 127.97 | 97.19 | 128.12 | 83.66 | 126.38 | 111.85 | 118.28 | 85.70 | 127.13 | 111.60 | 123.16 |
Intensity Change Log 2 | — | 0.059061 | 0.246674 | 0.320709 | — | 0.118323 | 0.392321 | 0.364584 | — | 0.088315 | 0.319660 | 0.342309 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000111 | 0.000060 | 0.001547 | 0.004378 | 0.000114 | 0.000137 | 0.000913 | 0.003182 | 0.000112 | 0.000101 | 0.000955 | 0.003738 |
Bud Neck | 0.016092 | 0.011333 | 0.014375 | 0.017040 | 0.013483 | 0.012616 | 0.015510 | 0.018687 | 0.015080 | 0.012025 | 0.015436 | 0.017920 |
Bud Site | 0.011121 | 0.011506 | 0.016620 | 0.067829 | 0.008646 | 0.010960 | 0.019595 | 0.037400 | 0.010161 | 0.011211 | 0.019400 | 0.051562 |
Cell Periphery | 0.000075 | 0.000126 | 0.000181 | 0.000110 | 0.000078 | 0.000361 | 0.000035 | 0.000070 | 0.000076 | 0.000253 | 0.000044 | 0.000089 |
Cytoplasm | 0.095338 | 0.080184 | 0.133495* | 0.261835* | 0.074158 | 0.041663 | 0.114358* | 0.249745* | 0.087121 | 0.059412 | 0.115617* | 0.255372* |
Cytoplasmic Foci | 0.201176* | 0.066273 | 0.188112* | 0.109753 | 0.167960 | 0.050405 | 0.170208 | 0.145745 | 0.188289* | 0.057716 | 0.171386 | 0.128993 |
Eisosomes | 0.000069 | 0.000026 | 0.000116 | 0.000041 | 0.000055 | 0.000048 | 0.000019 | 0.000041 | 0.000063 | 0.000038 | 0.000025 | 0.000041 |
Endoplasmic Reticulum | 0.002272 | 0.002773 | 0.008274 | 0.001520 | 0.001294 | 0.002761 | 0.001150 | 0.001052 | 0.001892 | 0.002766 | 0.001618 | 0.001270 |
Endosome | 0.245482* | 0.118103 | 0.164394 | 0.084760 | 0.205652* | 0.140334 | 0.203106* | 0.129863 | 0.230029* | 0.130090 | 0.200559* | 0.108870 |
Golgi | 0.026102 | 0.003087 | 0.006252 | 0.009977 | 0.024825 | 0.006745 | 0.018547 | 0.008736 | 0.025606 | 0.005060 | 0.017738 | 0.009314 |
Lipid Particles | 0.011763 | 0.004921 | 0.026449 | 0.004788 | 0.008810 | 0.003811 | 0.008737 | 0.004492 | 0.010617 | 0.004323 | 0.009902 | 0.004630 |
Mitochondria | 0.014569 | 0.003150 | 0.001120 | 0.002824 | 0.012011 | 0.003991 | 0.008212 | 0.004607 | 0.013576 | 0.003603 | 0.007745 | 0.003778 |
Mitotic Spindle | 0.002944 | 0.001221 | 0.046151 | 0.079770 | 0.002430 | 0.001582 | 0.063335 | 0.043978 | 0.002744 | 0.001416 | 0.062205 | 0.060637 |
None | 0.000283 | 0.000898 | 0.001975 | 0.000731 | 0.000394 | 0.000345 | 0.000249 | 0.000808 | 0.000326 | 0.000599 | 0.000362 | 0.000772 |
Nuclear Periphery | 0.000182 | 0.000499 | 0.004138 | 0.001008 | 0.000278 | 0.000193 | 0.001763 | 0.001264 | 0.000219 | 0.000334 | 0.001919 | 0.001145 |
Nuclear Periphery Foci | 0.000258 | 0.000342 | 0.020538 | 0.002937 | 0.000353 | 0.000228 | 0.001702 | 0.003130 | 0.000295 | 0.000280 | 0.002941 | 0.003040 |
Nucleolus | 0.000621 | 0.001669 | 0.005688 | 0.001102 | 0.000928 | 0.000591 | 0.001479 | 0.001254 | 0.000740 | 0.001088 | 0.001756 | 0.001183 |
Nucleus | 0.004894 | 0.012302 | 0.081954 | 0.024182 | 0.005154 | 0.005732 | 0.010956 | 0.020748 | 0.004995 | 0.008759 | 0.015627 | 0.022346 |
Peroxisomes | 0.005030 | 0.002287 | 0.022157 | 0.014552 | 0.007215 | 0.003283 | 0.010192 | 0.022830 | 0.005877 | 0.002824 | 0.010979 | 0.018977 |
Vacuole | 0.337482* | 0.649611* | 0.252023* | 0.306024* | 0.442830* | 0.686941* | 0.341423* | 0.298308* | 0.378353* | 0.669740* | 0.335541* | 0.301900* |
Vacuole Periphery | 0.024137 | 0.029630 | 0.004440 | 0.004840 | 0.023333 | 0.027275 | 0.008512 | 0.004060 | 0.023825 | 0.028360 | 0.008244 | 0.004423 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 3.48 | -5.16 | -4.52 | -4.61 | -1.30 | -0.70 | -3.90 | -2.53 | -2.50 | -1.46 | 0.48 | -4.45 | -4.49 | -4.50 | -2.54 |
Bud Neck | 3.66 | 0.35 | 0.90 | -2.67 | 0.05 | 0.57 | -3.75 | -3.20 | -3.51 | -0.38 | 3.04 | -2.87 | -1.68 | -4.39 | 0.91 |
Bud Site | -0.29 | -1.88 | -9.82 | -9.27 | -5.54 | -1.81 | -7.74 | -7.92 | -6.44 | -3.74 | -1.11 | -6.97 | -12.34 | -11.19 | -7.66 |
Cell Periphery | -4.71 | -6.29 | -1.83 | 3.53 | 5.60 | -5.44 | 5.21 | 2.28 | 5.88 | -5.50 | -6.24 | 7.23 | 1.08 | 6.44 | -6.67 |
Cytoplasm | 2.53 | -2.99 | -21.76 | -21.07 | -8.34 | 7.67 | -12.80 | -24.46 | -29.03 | -16.43 | 7.89 | -12.05 | -32.26 | -35.51 | -21.09 |
Cytoplasmic Foci | 26.71 | 2.65 | 25.21 | -2.36 | 7.56 | 21.84 | 2.89 | 8.46 | -13.77 | 7.27 | 36.10 | 8.71 | 23.12 | -11.89 | 14.21 |
Eisosomes | 11.42 | -5.71 | 8.82 | -4.07 | 9.06 | 0.91 | 9.57 | 4.27 | 1.46 | -8.12 | 5.70 | 14.25 | 9.62 | 0.12 | -6.11 |
Endoplasmic Reticulum | -1.37 | -6.02 | 3.05 | 4.60 | 6.86 | -5.72 | 0.24 | 2.09 | 7.17 | 2.18 | -3.74 | 0.60 | 4.00 | 8.32 | 4.24 |
Endosome | 21.29* | 7.86 | 39.99* | 13.24 | 8.04 | 9.49 | 6.05 | 18.26 | 7.85 | 14.57 | 21.94* | 13.59 | 39.49* | 14.09 | 22.81 |
Golgi | 17.30 | 13.41 | 14.57 | -0.93 | 0.87 | 8.66 | 5.14 | 9.63 | 1.11 | 6.98 | 17.22 | 8.85 | 17.24 | 0.44 | 8.43 |
Lipid Particles | 7.91 | -3.44 | 10.48 | 0.40 | 5.36 | 5.62 | -0.44 | 5.82 | -0.87 | 10.91 | 10.31 | 0.64 | 12.15 | -0.31 | 12.06 |
Mitochondria | 11.25 | 14.55 | 13.85 | 2.73 | -0.93 | 6.34 | 3.65 | 6.57 | 1.00 | 5.28 | 12.55 | 8.00 | 13.66 | 2.18 | 8.35 |
Mitotic Spindle | 1.91 | -4.20 | -13.06 | -13.34 | -1.92 | 0.90 | -20.63* | -10.49 | -10.80 | 5.81 | 2.08 | -21.29 | -16.68 | -17.09 | 2.73 |
None | -2.26 | -3.27 | -5.22 | 0.05 | 2.14 | 0.34 | 1.01 | -2.86 | -3.23 | -5.25 | -1.80 | -0.94 | -5.80 | -1.77 | -5.39 |
Nuclear Periphery | -2.87 | -7.34 | -12.36 | -7.93 | 4.40 | 1.22 | -14.08 | -5.59 | -6.19 | 3.37 | -1.92 | -17.01 | -10.84 | -9.57 | 4.64 |
Nuclear Periphery Foci | -0.64 | -6.15 | -8.85 | -8.96 | 5.40 | 1.04 | -8.07 | -4.21 | -4.42 | -1.17 | 0.11 | -9.50 | -7.88 | -8.01 | 1.24 |
Nucleolus | -4.30 | -5.17 | -4.27 | 1.90 | 4.69 | 1.39 | -3.52 | -2.50 | -4.39 | 1.15 | -1.77 | -9.15 | -5.06 | -1.94 | 4.83 |
Nucleus | -4.60 | -7.63 | -16.04 | -9.63 | 4.65 | -0.68 | -11.86 | -14.80 | -12.46 | -7.63 | -4.00 | -14.18 | -21.87 | -15.58 | -7.21 |
Peroxisomes | 6.89 | -3.73 | -4.27 | -7.12 | 2.73 | 5.25 | -3.19 | -5.21 | -7.79 | -3.69 | 7.58 | -7.21 | -7.00 | -10.10 | -1.93 |
Vacuole | -29.32* | 3.85 | -7.12 | 18.98 | -7.43 | -21.08 | 8.33 | 7.47 | 28.95 | 0.35 | -37.73* | 3.09 | -0.73 | 33.94 | -3.47 |
Vacuole Periphery | -3.45 | 21.70 | 25.98* | 18.60 | 2.16 | -2.69 | 15.78 | 19.35 | 20.80 | 8.98 | -4.29 | 26.92 | 32.86 | 27.80 | 10.71 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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