Standard name | |
---|---|
Human Ortholog | |
Description | Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 4 | 2 | 1 | 2 | 3 | 2 | 2 | 2 | 2 | 0 | 2 | 1 | 0 | 5 | 3 | 0 | 0 | 0 | 1 | 0 | 4 |
Bud | 3 | 2 | 3 | 2 | 9 | 4 | 18 | 12 | 18 | 20 | 10 | 7 | 0 | 1 | 1 | 1 | 1 | 2 | 9 | 1 | 1 | 8 | 17 | 25 |
Bud Neck | 0 | 0 | 4 | 2 | 3 | 2 | 6 | 6 | 8 | 5 | 3 | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 5 | 15 |
Bud Site | 3 | 0 | 0 | 6 | 5 | 13 | 19 | 15 | 21 | 18 | 6 | 6 | 0 | 1 | 1 | 1 | 2 | 3 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 5 | 8 | 8 | 25 | 23 | 19 | 14 | 16 | 17 | 8 | 10 | 45 | 125 | 120 | 27 | 35 | 26 | 2 | 1 | 3 | 4 | 8 | 14 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 7 | 1 | 0 | 0 | 0 | 1 | 1 | 4 |
Endosome | 93 | 56 | 12 | 32 | 85 | 68 | 42 | 20 | 29 | 42 | 13 | 28 | 90 | 229 | 247 | 112 | 114 | 101 | 31 | 16 | 10 | 26 | 55 | 67 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 2 | 4 |
Mitochondria | 34 | 6 | 1 | 4 | 5 | 17 | 45 | 20 | 61 | 54 | 61 | 25 | 0 | 0 | 0 | 13 | 13 | 10 | 1 | 0 | 0 | 2 | 4 | 7 |
Nucleus | 18 | 3 | 2 | 13 | 26 | 12 | 15 | 8 | 18 | 12 | 8 | 10 | 21 | 47 | 32 | 7 | 6 | 6 | 9 | 7 | 2 | 18 | 23 | 33 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 |
Nucleolus | 232 | 79 | 88 | 87 | 188 | 149 | 164 | 106 | 149 | 137 | 91 | 84 | 100 | 142 | 118 | 113 | 143 | 105 | 10 | 3 | 2 | 9 | 14 | 26 |
Peroxisomes | 6 | 1 | 0 | 1 | 10 | 8 | 5 | 1 | 4 | 6 | 2 | 4 | 0 | 0 | 13 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 176 | 49 | 139 | 162 | 366 | 254 | 240 | 175 | 177 | 241 | 92 | 121 | 187 | 328 | 248 | 131 | 163 | 136 | 274 | 119 | 172 | 209 | 251 | 327 |
Vac/Vac Membrane | 13 | 13 | 5 | 5 | 9 | 2 | 7 | 2 | 4 | 5 | 5 | 5 | 13 | 25 | 32 | 28 | 38 | 20 | 4 | 2 | 2 | 6 | 12 | 18 |
Unique Cell Count | 329 | 122 | 162 | 211 | 459 | 347 | 358 | 253 | 317 | 359 | 188 | 188 | 312 | 620 | 531 | 238 | 303 | 243 | 355 | 157 | 202 | 304 | 413 | 581 |
Labelled Cell Count | 585 | 214 | 263 | 327 | 734 | 554 | 582 | 384 | 507 | 559 | 301 | 305 | 461 | 903 | 814 | 440 | 534 | 416 | 355 | 157 | 202 | 304 | 413 | 581 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 7.8 | 6.7 | 6.5 | 6.5 | 5.3 | 5.1 | 5.6 | 4.8 | 5.2 | 4.7 | 5.4 | 6.9 | 7.0 | 7.1 | 9.1 | 9.8 | 9.3 | 7.0 | 7.2 | 7.6 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.1 | 1.4 | 1.6 | 1.1 | 1.1 | 1.1 | 0.9 | 1.1 | 0.8 | 1.2 | 1.0 | 0.9 | 1.0 | 1.8 | 2.3 | 1.8 | 1.2 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.04 | -0.34 | -0.39 | -0.27 | -0.48 | -0.37 | -0.5 | -0.32 | 0.03 | 0.07 | 0.08 | 0.43 | 0.54 | 0.47 | 0.05 | 0.11 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1.7 | 1.5 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3.0 | 3.2 | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | 0.2 | 0.7 | 0.2 | 0.3 | 0.1 | -0.1 | -0.3 | 0.2 | 3.1 | 4.6 | 5.1 | 2.2 | 2.3 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 2.3 | 3.3 | 3.5 | 1.5 | 0.2 | 0 | 1.5 | 0 | 2.2 | 5.4 | 7.2 | 9.0 | 8.4 | 7.0 | 7.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.4 | 4.4 | 3.3 | 0 | 5.0 | 0 | 4.5 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Nucleus | 2.4 | 2.3 | 0 | 1.8 | 0 | 2.3 | 0 | 0 | 2.1 | 2.6 | 3.0 | 2.5 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.5 | -2.9 | -2.4 | -1.8 | -2.5 | 0 | -3.4 | 0 | -1.8 | -4.7 | -7.8 | -7.8 | -1.3 | -1.5 | -2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.2 | -1.7 | -3.2 | -4.5 | -3.9 | 0 | -4.4 | 0 | -4.6 | -5.8 | -7.6 | -8.8 | -6.4 | -6.9 | -6.3 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 0.6 | 1.5 | 3.1 | 3.4 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9877 | 1.2203 | 1.1642 | 1.0105 | 0.5937 | 0.9619 | 3.1946 | 2.6688 | 3.4923 | 2.7482 | 2.0176 | 2.835 | 2.531 | 2.1064 | 1.8859 | 2.2206 | 2.42 | 2.322 |
Actin | 0.0126 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0124 | 0.0001 | 0.0067 | 0.0003 | 0.0019 | 0.0027 |
Bud | 0.0001 | 0.0011 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0 | 0 | 0 | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0014 | 0.0028 | 0.0021 | 0.0039 | 0.0171 | 0.0021 | 0.0002 | 0.0011 | 0.0006 | 0.0014 | 0.0004 | 0.0004 | 0.0015 | 0.0019 | 0.0071 | 0.0005 | 0.0009 | 0.0013 |
Bud Periphery | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Site | 0.0012 | 0.0132 | 0.0018 | 0.0008 | 0.0028 | 0.0002 | 0.0001 | 0.0008 | 0.0011 | 0.0001 | 0 | 0 | 0.0005 | 0.0039 | 0.0033 | 0.0001 | 0.0002 | 0.0003 |
Cell Periphery | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0023 | 0.0105 | 0.0008 | 0.0005 | 0.0005 | 0.0015 | 0.0021 | 0.0013 | 0.0011 | 0.0001 | 0 | 0.0002 | 0.0009 | 0.0064 | 0.0006 | 0.0018 | 0.0135 | 0.0092 |
Cytoplasmic Foci | 0.0299 | 0.0111 | 0.0058 | 0.069 | 0.1353 | 0.0084 | 0.0017 | 0.0033 | 0.0018 | 0.0449 | 0.0091 | 0.0036 | 0.0189 | 0.009 | 0.0104 | 0.032 | 0.111 | 0.0156 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0016 | 0.0008 |
Endosome | 0.0065 | 0.0077 | 0.001 | 0.013 | 0.01 | 0.0006 | 0.0004 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.003 | 0.0014 | 0.0013 | 0.0008 | 0.0117 | 0.0027 |
Golgi | 0.0008 | 0.0008 | 0.0004 | 0.0034 | 0.0036 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0012 | 0.0004 | 0.0009 | 0.0004 | 0.0034 | 0.0004 |
Lipid Particles | 0.008 | 0.0041 | 0.0036 | 0.0232 | 0.0156 | 0.0024 | 0.0002 | 0.0001 | 0.0002 | 0.001 | 0.0024 | 0.0004 | 0.0019 | 0.0013 | 0.0029 | 0.0007 | 0.0168 | 0.0009 |
Mitochondria | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0097 | 0.0001 | 0.0001 | 0.0003 |
None | 0.0005 | 0.0022 | 0.0004 | 0.0002 | 0.0002 | 0.0009 | 0.0001 | 0.0003 | 0.0011 | 0.0001 | 0 | 0 | 0.0005 | 0.0006 | 0.0006 | 0.0009 | 0.0168 | 0.0013 |
Nuclear Periphery | 0.0063 | 0.0052 | 0.0011 | 0.0017 | 0.0003 | 0.0009 | 0.0005 | 0.0022 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0097 | 0.0022 | 0.0007 | 0.0025 | 0.0027 | 0.0025 |
Nucleolus | 0.0385 | 0.0569 | 0.061 | 0.0252 | 0.071 | 0.037 | 0.0074 | 0.044 | 0.034 | 0.0014 | 0.0211 | 0.0092 | 0.018 | 0.037 | 0.024 | 0.0029 | 0.0021 | 0.0125 |
Nucleus | 0.0701 | 0.1804 | 0.0783 | 0.1647 | 0.0097 | 0.0225 | 0.026 | 0.0804 | 0.031 | 0.001 | 0.0017 | 0.0094 | 0.0171 | 0.1003 | 0.0391 | 0.0253 | 0.0013 | 0.0363 |
Peroxisomes | 0.0009 | 0.0008 | 0.0013 | 0.0064 | 0.0097 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0026 | 0.0005 | 0.0003 | 0.0008 | 0.0008 | 0.0012 | 0.0027 | 0.0098 | 0.0012 |
Punctate Nuclear | 0.8185 | 0.6977 | 0.8406 | 0.6857 | 0.7223 | 0.9225 | 0.9611 | 0.8653 | 0.9271 | 0.9461 | 0.9643 | 0.976 | 0.9119 | 0.8324 | 0.8907 | 0.9287 | 0.8056 | 0.9113 |
Vacuole | 0.0016 | 0.003 | 0.0004 | 0.0017 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0006 | 0.0002 | 0.0001 | 0.0004 | 0.0005 |
Vacuole Periphery | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.7902 | 12.14 | 6.0056 | 11.7941 | 8.0701 | 3.3932 | 13.5534 | 13.4625 | 9.0094 | 11.0707 |
Translational Efficiency | 1.5407 | 0.8259 | 1.6164 | 0.9952 | 1.2686 | 3.8424 | 0.9856 | 1.0657 | 1.5312 | 1.2318 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1150 | 1172 | 1559 | 1158 | 1946 | 2208 | 1532 | 1117 | 3096 | 3380 | 3091 | 2275 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 806.39 | 828.06 | 907.66 | 1021.63 | 777.64 | 829.51 | 1001.53 | 1036.58 | 788.32 | 829.01 | 954.19 | 1028.97 |
Standard Deviation | 108.15 | 134.12 | 141.32 | 157.22 | 100.08 | 111.98 | 135.88 | 152.03 | 104.08 | 120.12 | 146.38 | 154.88 |
Intensity Change Log 2 | — | 0.038258 | 0.170674 | 0.341323 | — | 0.093157 | 0.365031 | 0.414657 | — | 0.065470 | 0.269361 | 0.377791 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000574 | 0.001343 | 0.000202 | 0.000193 | 0.000469 | 0.000556 | 0.000390 | 0.000418 | 0.000508 | 0.000829 | 0.000295 | 0.000303 |
Bud Neck | 0.007425 | 0.007826 | 0.002871 | 0.001830 | 0.009200 | 0.011490 | 0.002155 | 0.001274 | 0.008540 | 0.010220 | 0.002516 | 0.001557 |
Bud Site | 0.005285 | 0.012617 | 0.002543 | 0.008818 | 0.006058 | 0.009889 | 0.004314 | 0.013659 | 0.005771 | 0.010835 | 0.003421 | 0.011195 |
Cell Periphery | 0.000244 | 0.000251 | 0.000046 | 0.000041 | 0.000238 | 0.000159 | 0.000099 | 0.000040 | 0.000240 | 0.000191 | 0.000072 | 0.000040 |
Cytoplasm | 0.062735 | 0.040866 | 0.006897 | 0.016273 | 0.051642 | 0.051728 | 0.047016 | 0.012467 | 0.055762 | 0.047961 | 0.026781 | 0.014404 |
Cytoplasmic Foci | 0.063251 | 0.058316 | 0.003084 | 0.006519 | 0.048539 | 0.033437 | 0.007619 | 0.006699 | 0.054004 | 0.042064 | 0.005332 | 0.006608 |
Eisosomes | 0.000111 | 0.000083 | 0.000010 | 0.000004 | 0.000094 | 0.000054 | 0.000018 | 0.000004 | 0.000100 | 0.000064 | 0.000014 | 0.000004 |
Endoplasmic Reticulum | 0.002677 | 0.003514 | 0.000490 | 0.000299 | 0.002428 | 0.001773 | 0.001514 | 0.000163 | 0.002521 | 0.002377 | 0.000998 | 0.000232 |
Endosome | 0.009046 | 0.012699 | 0.001409 | 0.003352 | 0.009094 | 0.011109 | 0.003486 | 0.000909 | 0.009076 | 0.011661 | 0.002438 | 0.002153 |
Golgi | 0.007505 | 0.009513 | 0.001225 | 0.006458 | 0.004442 | 0.004331 | 0.001082 | 0.001575 | 0.005580 | 0.006128 | 0.001154 | 0.004061 |
Lipid Particles | 0.008242 | 0.008215 | 0.000328 | 0.000611 | 0.012570 | 0.003626 | 0.001000 | 0.000339 | 0.010962 | 0.005218 | 0.000661 | 0.000478 |
Mitochondria | 0.010863 | 0.004186 | 0.000449 | 0.002290 | 0.004125 | 0.002064 | 0.000390 | 0.004407 | 0.006628 | 0.002800 | 0.000420 | 0.003329 |
Mitotic Spindle | 0.288954* | 0.487010* | 0.930012* | 0.920704* | 0.356885* | 0.521470* | 0.794186* | 0.940893* | 0.331652* | 0.509521* | 0.862692* | 0.930617* |
None | 0.017687 | 0.015501 | 0.000856 | 0.000800 | 0.017062 | 0.015598 | 0.006374 | 0.001207 | 0.017294 | 0.015564 | 0.003591 | 0.001000 |
Nuclear Periphery | 0.001194 | 0.000784 | 0.000332 | 0.000604 | 0.001232 | 0.001232 | 0.000575 | 0.000198 | 0.001218 | 0.001076 | 0.000453 | 0.000405 |
Nuclear Periphery Foci | 0.129989 | 0.135776 | 0.016700 | 0.011167 | 0.118148 | 0.067172 | 0.012507 | 0.004982 | 0.122547 | 0.090961 | 0.014622 | 0.008130 |
Nucleolus | 0.208204 | 0.089396 | 0.014774 | 0.001001 | 0.202749 | 0.119950 | 0.011842 | 0.000550 | 0.204775 | 0.109355 | 0.013321 | 0.000780 |
Nucleus | 0.148373* | 0.089909 | 0.014743 | 0.006305 | 0.141946* | 0.131412* | 0.094368 | 0.005954 | 0.144333* | 0.117021 | 0.054207 | 0.006132 |
Peroxisomes | 0.021778 | 0.015594 | 0.001136 | 0.000792 | 0.009267 | 0.008997 | 0.001109 | 0.000905 | 0.013914 | 0.011285 | 0.001123 | 0.000848 |
Vacuole | 0.005117 | 0.005740 | 0.001443 | 0.007255 | 0.002545 | 0.003263 | 0.009776 | 0.002987 | 0.003501 | 0.004122 | 0.005573 | 0.005160 |
Vacuole Periphery | 0.000746 | 0.000860 | 0.000451 | 0.004684 | 0.001266 | 0.000690 | 0.000178 | 0.000370 | 0.001073 | 0.000749 | 0.000316 | 0.002566 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.75 | -0.75 | -1.81 | 2.51 | -1.39 | -5.30 | -9.83 | -3.83 | -2.21 | 1.63 | -7.37 | -8.16 | -4.13 | -0.16 | 0.01 |
Bud Neck | -2.70 | -2.98 | -4.26 | -1.86 | -1.43 | -3.90 | -5.99 | -2.85 | -0.18 | 1.90 | -4.74 | -6.34 | -5.06 | -1.44 | 0.32 |
Bud Site | -5.71 | -3.16 | -7.19 | -3.97 | -5.31 | -5.02 | -9.73 | -8.22 | -6.67 | -3.56 | -7.38 | -9.70 | -11.15 | -8.15 | -6.14 |
Cell Periphery | -1.47 | 6.93 | 5.65 | 7.32 | -1.83 | 1.12 | -0.59 | 3.10 | 2.44 | 3.59 | -0.02 | 4.42 | 5.82 | 6.37 | 1.57 |
Cytoplasm | -0.62 | 4.90 | -0.02 | 0.58 | -4.71 | -2.57 | -4.61 | -1.70 | 0.20 | 2.32 | -2.29 | -0.36 | -1.46 | 0.43 | -1.16 |
Cytoplasmic Foci | -0.51 | 11.94 | 9.65 | 10.51 | -3.88 | 1.55 | 13.38 | 9.33 | 8.57 | -3.26 | 0.96 | 18.07 | 13.64 | 13.66 | -4.98 |
Eisosomes | 9.14 | 26.08 | 28.47 | 19.08 | 4.28 | 10.49 | 23.53 | 30.74 | 24.05 | 9.04 | 14.01 | 35.22 | 41.78 | 30.97 | 9.59 |
Endoplasmic Reticulum | -2.35 | 7.64 | 8.67 | 8.86 | 1.21 | -0.02 | -4.30 | 5.45 | 5.62 | 7.54 | -1.53 | 0.95 | 9.31 | 9.90 | 6.52 |
Endosome | -3.03 | 8.68 | 5.71 | 7.40 | -1.81 | -0.04 | 7.07 | 10.34 | 8.06 | 3.24 | -1.67 | 11.20 | 10.83 | 10.22 | 0.61 |
Golgi | -0.60 | 13.75 | 5.38 | 5.61 | -0.99 | 1.07 | 18.07 | 13.54 | 12.59 | 1.89 | 0.42 | 21.93 | 9.54 | 9.21 | -0.30 |
Lipid Particles | 0.16 | 8.50 | 6.66 | 5.58 | -6.55 | 4.04 | 6.96 | 6.69 | 6.15 | -0.88 | 3.41 | 10.41 | 9.02 | 7.92 | -4.97 |
Mitochondria | 0.65 | 1.77 | 1.04 | 0.41 | -1.67 | -0.02 | 1.38 | -0.75 | -0.77 | -1.12 | 0.64 | 2.22 | 0.00 | -0.51 | -1.66 |
Mitotic Spindle | -8.77 | -36.33* | -34.04* | -23.49* | 0.28 | -8.08 | -24.64* | -36.77* | -29.76* | -10.22 | -11.79 | -42.89 | -49.69 | -37.97* | -7.37 |
None | 0.48 | 9.27 | 9.08 | 6.93 | -0.30 | -1.29 | 4.37 | 8.76 | 9.94 | 5.17 | -0.62 | 9.99 | 12.77 | 12.39 | 4.60 |
Nuclear Periphery | 2.78 | 1.81 | -2.42 | -4.00 | -3.41 | 1.35 | -2.18 | 2.79 | 1.77 | 4.09 | 2.72 | 0.04 | -0.73 | -2.34 | -0.68 |
Nuclear Periphery Foci | 3.42 | 21.00* | 21.90* | 18.64* | 2.18 | 10.83 | 22.13* | 27.23* | 19.02* | 5.99 | 10.63 | 30.71 | 34.40 | 25.74* | 5.61 |
Nucleolus | 11.66 | 21.62* | 27.36* | 19.08 | 11.90 | 4.64 | 28.03* | 35.79* | 33.80* | 13.38 | 9.84 | 35.38* | 44.91* | 38.11* | 17.78 |
Nucleus | 1.63 | 6.44 | 6.79 | 4.96 | 0.79 | -3.46 | -7.42 | 5.84 | 8.93 | 11.13 | -1.79 | -2.51 | 8.63 | 10.15 | 9.81 |
Peroxisomes | -0.40 | 8.65 | 9.34 | 12.30 | 2.07 | 0.46 | 10.47 | 11.62 | 12.45 | 2.96 | 0.16 | 13.20 | 14.59 | 17.46 | 3.44 |
Vacuole | -1.94 | -2.11 | -7.14 | -6.57 | -6.52 | -1.83 | -7.43 | -6.86 | -6.59 | -0.70 | -2.51 | -7.89 | -9.96 | -9.46 | -5.15 |
Vacuole Periphery | -1.36 | -0.13 | -2.41 | -2.23 | -2.37 | 0.17 | 0.39 | 0.09 | -0.06 | -0.30 | -0.29 | 0.17 | -2.29 | -2.27 | -2.38 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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