Standard name
Human Ortholog
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.99 0.97 0.94 0.86 0.92 – 0.87 0.85 0.99 1.0 1.0 0.98 0.99 0.99 0.98 0.95 0.97 0.93 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.13 0.06 – 0 0.07 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 4 4 – 3 8 0 0 0 0 0 0 0 0 0 0 0 1
Bud 2 1 2 2 1 0 0 1 0 – 2 0 3 1 1 0 1 1 1 0 0 2 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 1 2 1 1 6 10 13 7 – 6 16 3 1 3 0 0 0 0 0 0 0 0 0
Cytoplasm 516 162 206 236 348 300 422 427 304 – 186 241 263 373 418 482 357 431 493 150 198 160 197 244
Endoplasmic Reticulum 0 0 0 1 4 7 30 62 19 – 9 21 0 1 1 23 21 25 0 0 0 2 0 5
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Golgi 1 0 0 0 0 0 0 1 0 – 0 0 1 0 0 5 3 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 0 0 4 – 3 4 0 0 0 0 1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 2 3 0 – 2 0 2 0 0 1 0 0 0 2 0 1 6 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 1 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 0 0 0 1 0 0 – 8 5 1 0 0 11 5 4 2 2 2 1 2 5
Unique Cell Count 518 164 207 238 352 308 451 495 331 213 283 266 374 420 494 362 435 505 159 205 173 215 273
Labelled Cell Count 525 166 210 240 354 313 465 511 338 219 295 274 376 423 523 388 462 505 159 205 173 215 273


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 107.0 88.8 59.4 59.9 60.9 49.2 47.6 46.2 41.4 – 38.2 40.7 89.4 79.8 67.2 106.2 132.1 126.8 79.5 75.9 80.8
Std Deviation (1e-4) 20.2 29.3 12.6 13.5 14.0 11.1 11.6 11.2 11.2 – 11.8 11.2 21.2 16.3 12.7 32.8 36.6 35.9 21.9 21.1 26.6
Intensity Change (Log2) – – – 0.01 0.04 -0.27 -0.32 -0.36 -0.52 – -0.64 -0.55 0.59 0.43 0.18 0.84 1.15 1.09 0.42 0.35 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 2.7 0 0 0 0 0 0
Cytoplasm -0.5 -0.8 -1.8 -3.4 -5.4 -3.9 – -5.0 -5.5 -0.8 0.4 0 -1.7 -1.0 -0.6
Endoplasmic Reticulum 0 0 0 3.8 5.3 3.5 – 0 4.0 0 0 0 3.2 3.5 3.5
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 99.2937 125.9881 117.3173 107.0663 117.643 109.4708 83.0031 124.3264 108.4151 102.7409 103.5618 114.1758 66.0972 96.2977 87.7381 72.8119 76.5564 86.6237
Actin 0.0002 0.0002 0.0001 0 0.0001 0.0021 0.0107 0 0.0034 0.005 0.0009 0.0001 0.0038 0.0001 0.0001 0.0013 0.0002 0
Bud 0.0004 0.0001 0.0002 0.0001 0.0002 0.0011 0.0012 0 0.0005 0.0003 0.0003 0 0.0007 0 0 0.0003 0.0001 0
Bud Neck 0.0002 0.0003 0.0004 0.0003 0.0006 0.0042 0.0003 0.0001 0.0002 0.0002 0.0002 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002
Bud Periphery 0.0001 0 0 0 0 0.001 0.0018 0 0.0002 0.0004 0.0002 0 0.0007 0 0 0.0001 0 0
Bud Site 0 0.0001 0 0 0 0.0001 0.0025 0 0.0008 0.0002 0.0003 0 0.0009 0 0 0.0006 0 0
Cell Periphery 0.0002 0.0003 0.0002 0 0.0001 0.0019 0.0021 0 0.0007 0.003 0.0013 0 0.0004 0 0 0.0001 0 0
Cytoplasm 0.9489 0.9891 0.9857 0.9922 0.9447 0.9025 0.834 0.9985 0.9725 0.9406 0.9334 0.9961 0.8991 0.9991 0.9985 0.9456 0.9528 0.9985
Cytoplasmic Foci 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0026 0 0.0011 0.0004 0.0021 0.0002 0.0004 0.0001 0.0001 0.009 0.0012 0.0001
Eisosomes 0 0 0 0 0 0 0.0013 0 0.0001 0.0004 0.0004 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0005 0.0017 0.0004 0.0001 0.001 0.0025 0.0098 0 0.0008 0.0008 0.0008 0.0001 0.0061 0 0.0001 0.0026 0.0022 0
Endosome 0.0002 0.0001 0.0001 0.0001 0.0002 0.0009 0.0082 0 0.0008 0.0014 0.0014 0 0.0026 0 0 0.003 0.0121 0
Golgi 0 0 0 0 0 0 0.0022 0 0.0002 0.0007 0.0004 0 0.0003 0 0 0.0003 0.0001 0
Lipid Particles 0 0 0 0 0 0.0001 0.003 0 0.0009 0.0005 0.0014 0 0.0001 0 0 0.0021 0 0
Mitochondria 0.0005 0.0001 0.0002 0 0.0002 0.0088 0.0199 0 0.0009 0.0209 0.0253 0 0.0091 0 0 0.0031 0.0011 0
None 0.0003 0.0001 0.0001 0 0.0002 0.0004 0.005 0.0001 0.0014 0.0009 0.0063 0.0001 0.001 0.0001 0.0001 0.0062 0.0003 0
Nuclear Periphery 0.0229 0.0019 0.0034 0.0012 0.0093 0.0192 0.0513 0.0002 0.0034 0.0046 0.0052 0.0006 0.0417 0.0001 0.0002 0.0065 0.0138 0.0002
Nucleolus 0 0.0001 0 0 0.0001 0.0004 0.002 0 0.0006 0.0033 0.0029 0 0.0003 0 0 0.0017 0 0
Nucleus 0.0195 0.0039 0.006 0.0037 0.0389 0.0157 0.0258 0.0009 0.0075 0.0051 0.0053 0.0018 0.0268 0.0004 0.0008 0.0086 0.0049 0.0008
Peroxisomes 0 0 0 0 0 0.0001 0.0004 0 0.0002 0.0001 0.0004 0 0.0001 0 0 0.0004 0 0
Punctate Nuclear 0 0 0 0 0 0.0001 0.0017 0 0.0003 0.0002 0.0005 0.0001 0.0004 0 0 0.0054 0.0001 0
Vacuole 0.0039 0.0016 0.0023 0.0015 0.0031 0.025 0.0065 0.0001 0.0027 0.0039 0.0038 0.0001 0.0027 0 0 0.0016 0.0041 0.0001
Vacuole Periphery 0.0018 0.0004 0.0008 0.0004 0.0011 0.0138 0.0077 0 0.0009 0.0072 0.0072 0 0.0028 0 0 0.0011 0.0066 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 124.8843 109.9686 102.6732 109.3716 120.9338 137.8881 106.6149 101.6434 117.7668 119.494
Translational Efficiency 0.4991 0.4711 0.4504 0.5588 0.5499 0.4516 0.4486 0.4046 0.4033 0.4248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Pyc2

Pyc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pyc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available