ORF
Human Ortholog
Description Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0 0 0 – 0 0 0 0 0.07 0 0.06 – 0.09 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 1.0 0.99 0.96 0.96 – 0.87 0.85 0.81 0.84 0.99 0.99 1.0 – 0.97 0.97 0.93 0.94 0.91 0.78 0.76 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.08 – 0.2 0.22 0.31 0.23 0 0 0 – 0 0 0 0 0 0.06 0.05 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 0 1 0 – 2 2 1 1 0 0 0 – 2 0 0 2 1 3 5 7
Bud 1 3 0 1 1 3 3 – 6 5 4 3 4 1 2 – 1 0 0 2 1 2 4 8
Bud Neck 0 0 6 0 1 1 3 – 1 3 0 2 0 0 0 – 0 0 1 1 1 3 4 3
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 9 20 15 13 4 9 6 – 7 6 8 6 18 9 20 – 44 23 0 0 1 3 2 2
Cytoplasm 257 478 332 250 336 348 376 – 290 287 207 188 267 272 336 – 461 338 242 455 301 201 195 190
Endoplasmic Reticulum 2 6 2 0 0 0 0 – 1 2 0 3 0 3 0 – 14 13 3 6 4 6 13 12
Endosome 0 0 0 0 1 0 1 – 0 0 0 0 0 0 1 – 4 2 4 2 0 1 2 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 3 1 1 0 0 1
Mitochondria 1 3 0 2 9 32 32 – 66 76 80 51 1 0 1 – 7 4 0 2 9 16 13 8
Nucleus 0 1 1 1 1 3 0 – 3 3 2 0 0 0 0 – 1 4 0 0 1 0 0 0
Nuclear Periphery 0 0 1 0 1 0 0 – 1 0 0 0 0 0 0 – 0 0 1 0 1 0 0 1
Nucleolus 0 0 0 1 0 0 0 – 2 4 2 0 0 0 0 – 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 – 0 2 0 0 0 1 1 – 0 1 0 0 0 0 1 2
Vac/Vac Membrane 0 2 0 0 2 0 0 – 2 0 1 0 0 0 0 – 12 3 0 3 1 13 5 6
Unique Cell Count 260 487 335 251 340 363 392 335 339 257 225 269 276 336 475 350 261 482 330 260 256 252
Labelled Cell Count 270 515 358 268 356 397 421 381 390 305 254 290 286 362 546 389 261 482 330 260 256 252


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.8 5.8 5.2 5.8 4.7 4.9 – 4.5 4.2 4.1 4.2 5.5 5.8 5.7 – 8.3 7.4 5.7 6.5 7.1
Std Deviation (1e-4) 1.0 0.8 1.0 0.9 1.3 1.0 1.2 – 1.2 1.0 1.0 0.7 1.1 1.1 0.9 – 1.5 1.4 1.4 1.9 1.7
Intensity Change (Log2) – – – -0.17 -0.0 -0.32 -0.27 – -0.36 -0.48 -0.52 -0.46 -0.09 -0.02 -0.04 – 0.51 0.34 -0.02 0.14 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0.4 -2.6 0 0 – 0 0 0 0 1.2 -0.8 0.9 – 2.6 1.2
Cytoplasm 0.7 -0.4 -2.7 -2.7 – -6.3 -6.8 -7.8 -7.0 0.2 -0.6 1.7 – -2.0 -2.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – 2.4 2.8
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5601 3.3473 2.8712 2.8589 2.8962 3.0429 1.3541 1.8625 1.5697 1.4104 1.4484 1.5856 1.2138 1.5305 1.3585 1.1632 1.3967 1.3911
Actin 0.0153 0.0006 0.0042 0.0014 0.0003 0.0118 0.022 0.0034 0.0088 0.0174 0.0608 0.0061 0.0398 0.0047 0.011 0.003 0.0134 0.008
Bud 0.0016 0.0045 0.0091 0.0006 0.0001 0.0002 0.0021 0.0063 0.0041 0.0036 0.0117 0.0018 0.0201 0.0505 0.0435 0.0442 0.0367 0.0347
Bud Neck 0.0003 0.0007 0.0056 0.0015 0.0001 0.0017 0.0109 0.0005 0.0033 0.0009 0.01 0.0018 0.0029 0.0012 0.0014 0.0009 0.007 0.0052
Bud Periphery 0.0015 0.005 0.0016 0.0008 0.0005 0.0006 0.003 0.0061 0.006 0.0192 0.0232 0.0064 0.0209 0.0196 0.0333 0.0209 0.0259 0.0334
Bud Site 0.0018 0.0114 0.0132 0.0366 0.0001 0.0002 0.023 0.0222 0.0233 0.0009 0.0137 0.0003 0.025 0.1129 0.054 0.0056 0.017 0.0039
Cell Periphery 0.002 0.0025 0.007 0.0019 0.0005 0.003 0.0013 0.0007 0.0006 0.0004 0.0007 0.0003 0.0018 0.0031 0.0022 0.0008 0.002 0.0011
Cytoplasm 0.4289 0.6305 0.5248 0.6066 0.3855 0.6575 0.5468 0.8266 0.7742 0.7238 0.4021 0.8201 0.3528 0.5375 0.5026 0.5239 0.4832 0.5224
Cytoplasmic Foci 0.0127 0.0042 0.0048 0.0113 0.0021 0.0038 0.0361 0.0082 0.0126 0.0183 0.0836 0.0162 0.0418 0.0113 0.0242 0.01 0.0237 0.0128
Eisosomes 0.0007 0.0003 0.0007 0.0004 0.0002 0.0004 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0005 0.0003 0.0003 0.0001 0.0004 0.0001
Endoplasmic Reticulum 0.0066 0.0021 0.0025 0.0022 0.002 0.0019 0.0134 0.0024 0.0037 0.0031 0.0041 0.0044 0.0059 0.0016 0.0031 0.0026 0.0088 0.0022
Endosome 0.0156 0.0009 0.0025 0.0011 0.0004 0.0008 0.0463 0.0023 0.007 0.0167 0.0896 0.0074 0.0189 0.0015 0.0101 0.0037 0.0252 0.0026
Golgi 0.003 0.0002 0.0004 0.0003 0.0001 0.0006 0.0108 0.0004 0.0061 0.0049 0.0484 0.0011 0.0042 0.0005 0.0012 0.0003 0.0102 0.0003
Lipid Particles 0.0067 0.0002 0.0006 0.0004 0.0002 0.0009 0.0151 0.0002 0.0049 0.0059 0.0135 0.0027 0.0043 0.0004 0.0018 0.0004 0.0082 0.0004
Mitochondria 0.0068 0.0001 0.0004 0.0001 0.0001 0.0004 0.0061 0.0004 0.0051 0.0018 0.0148 0.0007 0.002 0.0017 0.0035 0.0017 0.0109 0.001
None 0.4696 0.3319 0.4153 0.3304 0.6068 0.3132 0.2224 0.1156 0.1274 0.171 0.1867 0.1197 0.4342 0.2336 0.274 0.3641 0.2495 0.3538
Nuclear Periphery 0.0046 0.0003 0.0005 0.0002 0.0001 0.0003 0.0131 0.0003 0.0012 0.0019 0.0035 0.0014 0.0058 0.0012 0.0029 0.0032 0.0116 0.0022
Nucleolus 0.003 0.0002 0.0005 0.0002 0 0.0001 0.0011 0.0001 0.0003 0.0002 0.0009 0.0001 0.0008 0.0017 0.0019 0.001 0.0067 0.0006
Nucleus 0.0018 0.0008 0.0029 0.0006 0.0002 0.0003 0.0043 0.001 0.0028 0.0014 0.0021 0.0012 0.003 0.007 0.0067 0.0059 0.0173 0.0073
Peroxisomes 0.0115 0.0001 0.0002 0.0001 0 0.001 0.0092 0.0006 0.0049 0.002 0.0126 0.0052 0.0037 0.0016 0.0068 0.0003 0.003 0.0005
Punctate Nuclear 0.003 0.0019 0.0007 0.0018 0.0001 0.0003 0.0021 0.0003 0.0009 0.002 0.0072 0.001 0.0075 0.0048 0.0103 0.0036 0.0181 0.0044
Vacuole 0.002 0.0013 0.0023 0.0012 0.0003 0.0009 0.0084 0.0021 0.0022 0.004 0.0084 0.0015 0.0035 0.0031 0.0045 0.0033 0.0172 0.0026
Vacuole Periphery 0.0008 0.0001 0.0002 0.0001 0 0.0001 0.0023 0.0002 0.0004 0.0006 0.0024 0.0006 0.0007 0.0003 0.0007 0.0006 0.0042 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1643 6.2036 7.0404 10.3605 5.5573 12.2397 8.3941 12.327 11.8292 11.0901
Translational Efficiency 0.6332 0.7272 0.7024 0.643 0.8904 0.5577 0.6631 0.5325 0.571 0.5822

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1667 1805 367 2132 1371 675 422 749 3038 2480 789 2881

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 673.19 766.97 991.60 966.89 674.65 692.73 890.51 893.31 673.85 746.76 937.53 947.76
Standard Deviation 73.74 108.24 117.69 121.34 79.46 101.69 106.76 133.21 76.38 111.50 122.81 128.65
Intensity Change Log 2 — 0.188156 0.558745 0.522338 — 0.038154 0.400493 0.405022 — 0.115023 0.481702 0.464808

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000277 0.000666 0.000231 0.000979 0.000202 0.000635 0.000106 0.000266 0.000243 0.000658 0.000164 0.000794
Bud Neck 0.016856 0.025615 0.001845 0.005927 0.008737 0.020992 0.002247 0.002516 0.013192 0.024356 0.002060 0.005040
Bud Site 0.009224 0.028864 0.010275 0.056650 0.006302 0.021095 0.003446 0.034081 0.007906 0.026749 0.006622 0.050782
Cell Periphery 0.000197 0.000139 0.000248 0.000154 0.000158 0.000221 0.000213 0.000140 0.000179 0.000162 0.000229 0.000150
Cytoplasm 0.575487* 0.525588* 0.404953* 0.708876* 0.580330* 0.453394* 0.492900* 0.584817* 0.577673* 0.505939* 0.451991* 0.676623*
Cytoplasmic Foci 0.205639* 0.228497* 0.071370 0.028851 0.219488* 0.272990* 0.079098 0.071100 0.211889* 0.240607* 0.075503 0.039835
Eisosomes 0.000327 0.000151 0.000135 0.000024 0.000192 0.000293 0.000121 0.000048 0.000266 0.000189 0.000128 0.000030
Endoplasmic Reticulum 0.000713 0.000706 0.002318 0.000295 0.000685 0.000539 0.000735 0.000821 0.000700 0.000660 0.001471 0.000432
Endosome 0.012989 0.026311 0.014684 0.004252 0.017202 0.051952 0.008266 0.008741 0.014890 0.033290 0.011251 0.005419
Golgi 0.003656 0.007673 0.000162 0.003153 0.003649 0.008268 0.000186 0.000830 0.003653 0.007835 0.000175 0.002549
Lipid Particles 0.005481 0.003515 0.005265 0.000382 0.006903 0.007632 0.002509 0.000946 0.006123 0.004635 0.003791 0.000529
Mitochondria 0.002013 0.003419 0.000111 0.002644 0.002022 0.007043 0.000245 0.000087 0.002017 0.004406 0.000182 0.001979
Mitotic Spindle 0.000899 0.001783 0.000657 0.042889 0.001201 0.001335 0.002875 0.004202 0.001035 0.001661 0.001844 0.032832
None 0.003093 0.000990 0.002463 0.001441 0.002677 0.002277 0.004274 0.002213 0.002905 0.001340 0.003432 0.001642
Nuclear Periphery 0.000207 0.000250 0.001047 0.000273 0.000217 0.000251 0.000592 0.000250 0.000211 0.000250 0.000804 0.000267
Nuclear Periphery Foci 0.000354 0.000429 0.004432 0.001023 0.000716 0.000583 0.001481 0.002203 0.000518 0.000471 0.002854 0.001330
Nucleolus 0.000516 0.000434 0.000582 0.000176 0.000776 0.001268 0.000539 0.000175 0.000633 0.000661 0.000559 0.000176
Nucleus 0.069688 0.027180 0.120229 0.030292 0.063239 0.033218 0.160779* 0.021042 0.066777 0.028823 0.141918* 0.027887
Peroxisomes 0.002578 0.006875 0.000556 0.002017 0.002445 0.010713 0.001285 0.001275 0.002518 0.007919 0.000946 0.001824
Vacuole 0.088935 0.109030 0.358090* 0.106025 0.081868 0.102776 0.237890* 0.263816* 0.085746 0.107328 0.293800* 0.147047
Vacuole Periphery 0.000873 0.001886 0.000346 0.003679 0.000990 0.002523 0.000216 0.000429 0.000926 0.002059 0.000276 0.002834

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.50 -0.28 -2.97 0.07 -3.37 -2.59 1.83 -0.96 2.29 -3.24 -3.55 0.70 -3.49 0.82 -4.49
Bud Neck -4.92 14.44 11.53 14.02 -4.18 -5.80 9.74 9.55 9.28 -0.36 -8.27 17.21 13.38 16.64 -4.22
Bud Site -11.58 -0.24 -8.88 -0.68 -6.74 -7.22 3.27 -6.03 -1.19 -6.87 -14.31 1.22 -11.03 -1.17 -10.67
Cell Periphery 5.39 -1.48 5.39 2.47 5.69 -2.46 -2.15 4.72 4.46 5.26 1.88 -2.29 5.87 4.20 6.67
Cytoplasm 5.85 15.57 -9.16 -14.24 -20.30 10.42 9.26 4.80 -4.39 -4.30 10.29 17.12 -5.70 -14.40 -19.88
Cytoplasmic Foci -3.54 27.00 40.54 41.64 6.99 -5.90 23.86 27.50 25.59 1.02 -5.53 35.42 51.46 48.70 8.13
Eisosomes 7.86 8.95 14.36 15.77 15.42 -1.90 6.69 16.26 4.83 10.27 3.99 10.86 19.22 10.51 19.12
Endoplasmic Reticulum 0.07 -8.00 4.83 3.48 9.88 1.35 -3.52 0.20 -0.51 2.11 0.38 -8.43 4.47 2.81 10.72
Endosome -8.59 0.56 14.36 17.10 10.42 -8.77 6.71 7.44 11.79 0.60 -11.46 5.71 15.91 20.17 8.98
Golgi -6.67 10.08 4.33 9.85 -2.59 -4.18 7.08 5.62 7.61 -1.36 -7.81 11.92 5.90 12.27 -2.81
Lipid Particles 3.94 1.71 12.92 8.66 8.83 -0.72 7.81 10.86 7.92 6.28 3.25 6.95 16.93 11.68 11.37
Mitochondria -2.18 4.81 1.80 3.97 -3.60 -2.38 3.66 3.96 3.41 1.84 -3.23 5.93 3.08 5.08 -3.27
Mitotic Spindle -1.25 -0.71 -8.27 -7.87 -7.71 -0.05 -0.83 -2.74 -2.84 -1.14 -1.10 -1.10 -8.59 -8.23 -7.31
None 3.39 0.90 2.70 -2.43 0.56 0.47 -0.66 1.73 1.04 1.76 3.51 0.19 3.41 -0.51 1.80
Nuclear Periphery -0.27 -10.31 -2.80 -0.87 9.27 -0.60 -10.75 -1.13 -0.25 7.37 -0.35 -13.64 -3.39 -0.91 11.38
Nuclear Periphery Foci -0.61 -12.78 -2.63 -2.61 11.04 0.94 -4.35 -2.73 -2.95 -1.17 0.68 -12.65 -3.47 -3.89 7.16
Nucleolus 1.47 0.55 6.01 4.70 3.99 -1.56 2.26 4.75 4.26 4.95 -0.06 2.21 6.58 5.78 6.11
Nucleus 19.20 -6.17 10.31 -10.27 9.28 8.36 -11.46 15.91 3.77 17.39 20.53 -12.50 15.01 -6.84 17.67
Peroxisomes -8.54 9.83 5.13 11.71 -4.23 -4.87 3.18 4.68 5.74 -0.05 -9.21 6.64 6.75 11.66 -1.56
Vacuole -5.11 -22.83 -20.53 -16.22 14.83 -3.92 -16.79 -24.58 -21.09 -2.42 -6.82 -27.26 -30.54 -24.74 11.40
Vacuole Periphery -8.02 9.70 -1.19 0.84 -2.41 -5.66 7.51 6.07 8.70 -1.74 -9.39 11.75 -0.63 2.39 -2.56
ORF
Human Ortholog
Description Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YBR225W

YBR225W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ybr225w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available