Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 17 | 6 | 4 | 4 | 2 | 6 |
Bud | 18 | 1 | 15 | 16 | 9 | 12 | – | 26 | 13 | 17 | 19 | 25 | 4 | 1 | 3 | 2 | 2 | 0 | 5 | 5 | 1 | 1 | 1 | 3 |
Bud Neck | 3 | 0 | 9 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 3 | 1 | 2 | 6 | 3 |
Bud Site | 11 | 1 | 1 | 1 | 5 | 8 | – | 11 | 9 | 13 | 6 | 14 | 0 | 0 | 0 | 3 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 10 | 11 | 6 | 8 | 3 | 2 | – | 1 | 0 | 3 | 1 | 4 | 3 | 1 | 7 | 14 | 3 | 8 | 2 | 1 | 2 | 0 | 0 | 0 |
Cytoplasm | 189 | 86 | 96 | 31 | 28 | 31 | – | 52 | 17 | 18 | 19 | 8 | 55 | 81 | 111 | 48 | 5 | 22 | 24 | 11 | 5 | 1 | 4 | 3 |
Endoplasmic Reticulum | 3 | 2 | 1 | 1 | 2 | 1 | – | 1 | 0 | 1 | 2 | 5 | 6 | 4 | 2 | 11 | 5 | 8 | 17 | 7 | 4 | 1 | 2 | 4 |
Endosome | 83 | 35 | 3 | 4 | 9 | 23 | – | 2 | 1 | 0 | 0 | 0 | 18 | 16 | 60 | 47 | 25 | 31 | 77 | 18 | 14 | 12 | 8 | 11 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 11 | 7 | 3 | 1 | 3 | 3 |
Mitochondria | 415 | 130 | 169 | 181 | 207 | 278 | – | 354 | 386 | 419 | 385 | 516 | 50 | 35 | 48 | 28 | 10 | 21 | 631 | 281 | 210 | 80 | 111 | 110 |
Nucleus | 5 | 1 | 1 | 0 | 4 | 7 | – | 19 | 10 | 6 | 5 | 9 | 1 | 1 | 1 | 21 | 2 | 3 | 2 | 2 | 0 | 0 | 0 | 2 |
Nuclear Periphery | 2 | 2 | 0 | 1 | 1 | 2 | – | 2 | 7 | 11 | 11 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 4 | 0 | 0 | 0 | 0 | 2 | – | 5 | 14 | 8 | 11 | 17 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 17 | 10 | 4 | 3 | 11 | 14 | – | 3 | 2 | 3 | 2 | 4 | 5 | 0 | 34 | 30 | 16 | 35 | 12 | 2 | 7 | 3 | 3 | 3 |
SpindlePole | 34 | 10 | 17 | 7 | 8 | 18 | – | 27 | 10 | 11 | 6 | 4 | 3 | 4 | 6 | 31 | 14 | 30 | 12 | 3 | 4 | 0 | 3 | 2 |
Vac/Vac Membrane | 93 | 79 | 6 | 4 | 16 | 23 | – | 11 | 23 | 16 | 7 | 17 | 27 | 13 | 31 | 50 | 15 | 18 | 98 | 43 | 6 | 7 | 19 | 20 |
Unique Cell Count | 698 | 311 | 261 | 206 | 246 | 329 | 417 | 400 | 438 | 405 | 535 | 155 | 146 | 255 | 217 | 70 | 120 | 951 | 407 | 279 | 119 | 170 | 183 | |
Labelled Cell Count | 888 | 371 | 330 | 257 | 304 | 421 | 515 | 492 | 526 | 474 | 645 | 172 | 158 | 304 | 288 | 100 | 181 | 951 | 407 | 279 | 119 | 170 | 183 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 5.4 | 3.5 | 4.2 | 4.2 | 3.9 | – | 3.5 | 3.5 | 3.5 | 3.9 | 3.8 | 5.2 | 5.3 | 5.0 | 7.0 | 6.9 | 6.3 | 4.9 | 5.3 | 5.6 |
Std Deviation (1e-4) | 0.7 | 1.4 | 1.1 | 7.7 | 1.6 | 4.5 | – | 1.4 | 1.2 | 1.0 | 1.5 | 1.1 | 1.2 | 1.2 | 1.0 | 2.6 | 2.0 | 1.6 | 1.2 | 1.3 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.24 | 0.26 | 0.14 | – | 0.01 | -0.01 | 0.01 | 0.15 | 0.1 | 0.55 | 0.6 | 0.52 | 1.0 | 0.96 | 0.83 | 0.47 | 0.6 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.5 | -2.5 | -2.8 | -2.8 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | 2.3 | 0 | 0 |
Cytoplasm | -5.2 | -6.6 | -8.0 | – | -7.5 | -10.9 | -11.3 | -10.7 | -13.9 | -0.3 | 3.6 | 1.6 | -3.5 | -4.8 | -3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4.7 | 4.5 | 7.8 | 7.3 | 9.2 | 7.9 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -6.4 | -7.9 | -10.6 | -11.5 | -7.5 | -8.6 |
Nucleus | 0 | 0 | 0 | – | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.8 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.1 | 5.2 | 0 | 8.3 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.1 | -1.7 | -2.3 | 2.8 | 3.4 | 5.1 |
Vacuole | 0 | 2.3 | 2.6 | – | 0.3 | 2.1 | 1.0 | -0.5 | 0.7 | 5.5 | 3.0 | 4.3 | 7.0 | 0 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0167 | 4.7301 | 4.6652 | 4.107 | 4.4051 | 4.2697 | 2.971 | 2.1766 | 2.3867 | 2.3823 | 2.2016 | 2.3221 | 3.7916 | 4.0373 | 4.1598 | 3.9754 | 3.0879 | 3.5327 |
Actin | 0.0199 | 0.009 | 0.0066 | 0.0457 | 0.0082 | 0.0008 | 0.0356 | 0.0045 | 0.0134 | 0.0015 | 0.055 | 0.0032 | 0.0091 | 0.0038 | 0.0119 | 0.0031 | 0.0012 | 0.0018 |
Bud | 0.0011 | 0.004 | 0.0011 | 0.0003 | 0.0002 | 0.0002 | 0.001 | 0.0066 | 0.002 | 0.0009 | 0.0006 | 0.0002 | 0.0011 | 0.0009 | 0.0014 | 0.002 | 0.007 | 0.0002 |
Bud Neck | 0.0007 | 0.0008 | 0.0029 | 0.0008 | 0.0002 | 0.0006 | 0.0036 | 0.0005 | 0.0014 | 0.0003 | 0.0014 | 0.0006 | 0.0004 | 0.0007 | 0.0009 | 0.0005 | 0.0009 | 0.0014 |
Bud Periphery | 0.0022 | 0.0061 | 0.0022 | 0.0006 | 0.0005 | 0.0005 | 0.0022 | 0.0088 | 0.002 | 0.0041 | 0.0013 | 0.0006 | 0.003 | 0.0022 | 0.0033 | 0.007 | 0.0142 | 0.0008 |
Bud Site | 0.0052 | 0.0218 | 0.0081 | 0.0004 | 0.0009 | 0.0002 | 0.0079 | 0.0148 | 0.0102 | 0.0007 | 0.0012 | 0.0002 | 0.0041 | 0.0085 | 0.0113 | 0.0016 | 0.0061 | 0.0009 |
Cell Periphery | 0.0008 | 0.0014 | 0.001 | 0.0002 | 0.0001 | 0.0002 | 0.004 | 0.0015 | 0.0008 | 0.0004 | 0.0003 | 0.0002 | 0.0011 | 0.0008 | 0.0009 | 0.0011 | 0.0011 | 0.0004 |
Cytoplasm | 0.002 | 0.0025 | 0.0046 | 0.0003 | 0.0002 | 0.0008 | 0.0134 | 0.003 | 0.005 | 0.0006 | 0.0005 | 0.0003 | 0.0045 | 0.0013 | 0.003 | 0.0018 | 0.0145 | 0.0007 |
Cytoplasmic Foci | 0.0166 | 0.0132 | 0.0188 | 0.0063 | 0.019 | 0.0041 | 0.0329 | 0.0116 | 0.0187 | 0.0365 | 0.0205 | 0.0049 | 0.0154 | 0.0177 | 0.0107 | 0.0098 | 0.0173 | 0.0122 |
Eisosomes | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0038 | 0.0017 | 0.0007 | 0.0005 | 0.0002 | 0.0007 | 0.0081 | 0.0029 | 0.0004 | 0.0002 | 0.0014 | 0.0006 | 0.0026 | 0.0017 | 0.0016 | 0.0013 | 0.0006 | 0.0007 |
Endosome | 0.0666 | 0.0522 | 0.0496 | 0.0484 | 0.0344 | 0.0334 | 0.069 | 0.0454 | 0.0368 | 0.0284 | 0.0623 | 0.0237 | 0.0759 | 0.0705 | 0.0427 | 0.0307 | 0.0349 | 0.04 |
Golgi | 0.049 | 0.026 | 0.0323 | 0.0419 | 0.0241 | 0.0258 | 0.0526 | 0.0321 | 0.025 | 0.0469 | 0.059 | 0.0272 | 0.034 | 0.0447 | 0.0411 | 0.0202 | 0.0588 | 0.0436 |
Lipid Particles | 0.0243 | 0.0072 | 0.0081 | 0.008 | 0.0455 | 0.0056 | 0.0238 | 0.0059 | 0.0085 | 0.0063 | 0.0391 | 0.0063 | 0.0424 | 0.0109 | 0.0058 | 0.0019 | 0.0183 | 0.0103 |
Mitochondria | 0.7379 | 0.8029 | 0.8325 | 0.8221 | 0.8344 | 0.908 | 0.6687 | 0.8313 | 0.8377 | 0.8508 | 0.7066 | 0.9143 | 0.7548 | 0.791 | 0.8368 | 0.8938 | 0.7712 | 0.861 |
None | 0.0092 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0077 | 0.0008 | 0.002 | 0.0003 | 0.0006 | 0.0002 | 0.0011 | 0.0003 | 0.0006 | 0.0006 | 0.0009 | 0.0003 |
Nuclear Periphery | 0.0104 | 0.0018 | 0.0009 | 0.0002 | 0.0003 | 0.0084 | 0.0072 | 0.0003 | 0.0005 | 0.0001 | 0.0054 | 0.0009 | 0.0027 | 0.0015 | 0.0006 | 0.0009 | 0.0003 | 0.0004 |
Nucleolus | 0.0009 | 0.0012 | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.0021 | 0.0006 | 0.001 | 0.0002 | 0.0008 | 0.0001 | 0.0006 | 0.0029 | 0.0003 | 0.0003 | 0.0004 | 0.0001 |
Nucleus | 0.0046 | 0.0011 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0033 | 0.0006 | 0.0005 | 0.0002 | 0.0009 | 0.0002 | 0.0008 | 0.0011 | 0.0005 | 0.0006 | 0.0015 | 0.0001 |
Peroxisomes | 0.018 | 0.01 | 0.0071 | 0.0139 | 0.0209 | 0.0025 | 0.026 | 0.017 | 0.0138 | 0.0155 | 0.03 | 0.0117 | 0.0199 | 0.0179 | 0.0087 | 0.0069 | 0.0364 | 0.0164 |
Punctate Nuclear | 0.0021 | 0.0002 | 0.0008 | 0.0003 | 0.0015 | 0.0001 | 0.007 | 0.0007 | 0.0004 | 0.0003 | 0.0042 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0119 | 0.0182 | 0.0113 | 0.0043 | 0.0038 | 0.0031 | 0.0142 | 0.0063 | 0.0096 | 0.0031 | 0.0043 | 0.0016 | 0.0142 | 0.0119 | 0.009 | 0.0098 | 0.0103 | 0.0027 |
Vacuole Periphery | 0.0123 | 0.0178 | 0.009 | 0.0051 | 0.0049 | 0.0043 | 0.0087 | 0.0044 | 0.01 | 0.0027 | 0.0043 | 0.0027 | 0.0111 | 0.0091 | 0.0083 | 0.0057 | 0.0038 | 0.0056 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.3869 | 19.484 | 66.868 | 69.759 | 35.3238 | 57.1819 | 59.7163 | 63.1575 | 84.5882 | 57.9383 |
Translational Efficiency | 1.2233 | 2.9591 | 1.2358 | 1.4182 | 2.3621 | 2.4694 | 1.7515 | 2.0484 | 1.9352 | 1.9911 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2050 | 1465 | 133 | 1385 | 1907 | 990 | 1405 | 1435 | 3957 | 2455 | 1538 | 2820 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 659.12 | 716.41 | 841.85 | 793.09 | 646.56 | 728.92 | 771.59 | 778.71 | 653.07 | 721.45 | 777.67 | 785.77 |
Standard Deviation | 74.97 | 95.17 | 120.71 | 119.14 | 93.87 | 100.34 | 97.66 | 112.03 | 84.84 | 97.49 | 101.80 | 115.80 |
Intensity Change Log 2 | — | 0.120244 | 0.353022 | 0.266943 | — | 0.172976 | 0.255050 | 0.268302 | — | 0.146598 | 0.305339 | 0.267616 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000408 | 0.001004 | 0.002079 | 0.013956 | 0.001032 | 0.000599 | 0.002437 | 0.019277 | 0.000709 | 0.000840 | 0.002406 | 0.016663 |
Bud Neck | 0.050935 | 0.021635 | 0.014409 | 0.045516 | 0.027856 | 0.031857 | 0.065667 | 0.046756 | 0.039813 | 0.025757 | 0.061234 | 0.046147 |
Bud Site | 0.021046 | 0.035536 | 0.020196 | 0.148836* | 0.020376 | 0.019489 | 0.076604 | 0.187437* | 0.020723 | 0.029065 | 0.071726 | 0.168479* |
Cell Periphery | 0.000205 | 0.000275 | 0.000383 | 0.000340 | 0.000299 | 0.000333 | 0.000199 | 0.000683 | 0.000250 | 0.000299 | 0.000215 | 0.000515 |
Cytoplasm | 0.145621* | 0.025845 | 0.205469* | 0.218937* | 0.039318 | 0.025920 | 0.238894* | 0.197984* | 0.094391 | 0.025876 | 0.236004* | 0.208275* |
Cytoplasmic Foci | 0.324684* | 0.150413 | 0.111619 | 0.122570 | 0.150514 | 0.115899 | 0.110158 | 0.126571 | 0.240746* | 0.136495 | 0.110284 | 0.124606 |
Eisosomes | 0.000257 | 0.000124 | 0.000172 | 0.000218 | 0.000249 | 0.000154 | 0.000112 | 0.000265 | 0.000253 | 0.000136 | 0.000117 | 0.000242 |
Endoplasmic Reticulum | 0.001813 | 0.000746 | 0.013707 | 0.004479 | 0.001773 | 0.001608 | 0.005707 | 0.004112 | 0.001794 | 0.001094 | 0.006399 | 0.004292 |
Endosome | 0.069695 | 0.093143 | 0.039191 | 0.026968 | 0.076470 | 0.109113 | 0.025518 | 0.024839 | 0.072960 | 0.099583 | 0.026701 | 0.025885 |
Golgi | 0.037196 | 0.130255* | 0.002781 | 0.017786 | 0.071900 | 0.085836 | 0.008462 | 0.023550 | 0.053921 | 0.112343* | 0.007970 | 0.020719 |
Lipid Particles | 0.026856 | 0.006060 | 0.012180 | 0.003638 | 0.014849 | 0.008351 | 0.004425 | 0.002812 | 0.021069 | 0.006984 | 0.005096 | 0.003218 |
Mitochondria | 0.172759* | 0.393752* | 0.003739 | 0.039285 | 0.486075* | 0.464687* | 0.044695 | 0.070731 | 0.323756* | 0.422357* | 0.041154 | 0.055286 |
Mitotic Spindle | 0.003756 | 0.025361 | 0.011878 | 0.050258 | 0.003903 | 0.011881 | 0.043990 | 0.038093 | 0.003826 | 0.019925 | 0.041213 | 0.044068 |
None | 0.001396 | 0.001835 | 0.003318 | 0.000793 | 0.000980 | 0.001315 | 0.001575 | 0.000678 | 0.001195 | 0.001625 | 0.001726 | 0.000734 |
Nuclear Periphery | 0.000441 | 0.000252 | 0.003002 | 0.000709 | 0.000391 | 0.000366 | 0.001796 | 0.000340 | 0.000417 | 0.000298 | 0.001901 | 0.000521 |
Nuclear Periphery Foci | 0.000261 | 0.000370 | 0.007762 | 0.001376 | 0.000421 | 0.000185 | 0.000949 | 0.000539 | 0.000338 | 0.000295 | 0.001539 | 0.000950 |
Nucleolus | 0.001642 | 0.002806 | 0.007621 | 0.001157 | 0.001582 | 0.002853 | 0.003327 | 0.001212 | 0.001613 | 0.002825 | 0.003698 | 0.001185 |
Nucleus | 0.031435 | 0.004800 | 0.348622* | 0.056107 | 0.008381 | 0.010378 | 0.168305* | 0.039712 | 0.020325 | 0.007050 | 0.183898* | 0.047764 |
Peroxisomes | 0.028484 | 0.050936 | 0.016236 | 0.074319 | 0.035654 | 0.040470 | 0.041804 | 0.085550 | 0.031940 | 0.046716 | 0.039593 | 0.080034 |
Vacuole | 0.067364 | 0.034820 | 0.170224 | 0.165936 | 0.038601 | 0.045443 | 0.148903 | 0.123043 | 0.053502 | 0.039104 | 0.150747 | 0.144109 |
Vacuole Periphery | 0.013745 | 0.020030 | 0.005412 | 0.006815 | 0.019377 | 0.023261 | 0.006471 | 0.005819 | 0.016459 | 0.021333 | 0.006379 | 0.006308 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.59 | -5.51 | -9.63 | -9.00 | -7.96 | 2.32 | -4.93 | -11.07 | -11.39 | -10.16 | -0.81 | -7.50 | -14.64 | -14.42 | -12.77 |
Bud Neck | 10.12 | 10.13 | 2.44 | -7.85 | -8.24 | -1.16 | -10.68 | -6.74 | -4.04 | 4.86 | 6.41 | -6.67 | -2.55 | -8.72 | 4.90 |
Bud Site | -5.39 | 0.22 | -21.39 | -16.99 | -18.36 | 0.39 | -13.03 | -26.79* | -26.65* | -16.06 | -4.54 | -13.31 | -34.07 | -30.15 | -17.66 |
Cell Periphery | -2.43 | -3.33 | -4.23 | -0.83 | 1.31 | -0.72 | 4.67 | -2.14 | -1.87 | -2.92 | -1.85 | 3.03 | -2.77 | -2.09 | -3.41 |
Cytoplasm | 26.26 | -2.72 | -10.12 | -30.79 | -1.39 | 4.09 | -29.30 | -25.91 | -27.60 | 3.97 | 23.35 | -21.03 | -23.48 | -41.45 | 2.80 |
Cytoplasmic Foci | 26.83 | 16.72 | 33.33 | 6.52 | -0.14 | 5.82 | 8.65 | 4.93 | -1.22 | -3.41 | 22.39 | 27.46 | 27.41 | 4.31 | -3.00 |
Eisosomes | 9.37 | 3.63 | 3.21 | -6.79 | -1.08 | 4.54 | 7.55 | -0.31 | -4.73 | -7.59 | 9.54 | 11.76 | 1.43 | -7.79 | -9.90 |
Endoplasmic Reticulum | 5.32 | -6.20 | -5.92 | -8.59 | 4.71 | 0.25 | -9.01 | -5.03 | -3.80 | 3.41 | 2.37 | -12.04 | -7.77 | -8.86 | 4.86 |
Endosome | -5.74 | 5.01 | 16.30 | 18.79 | 2.36 | -5.85 | 17.15 | 17.47 | 16.99 | 0.17 | -8.00 | 21.15 | 23.92 | 25.45 | 0.96 |
Golgi | -19.67 | 18.63 | 10.64 | 24.96 | -6.95 | -2.76 | 22.37 | 15.71 | 14.23 | -7.39 | -16.38 | 26.49 | 18.62 | 28.15 | -8.05 |
Lipid Particles | 11.31 | 6.45 | 14.36 | 2.39 | 4.92 | 4.22 | 8.63 | 10.05 | 5.19 | 4.41 | 11.60 | 15.25 | 17.48 | 5.13 | 5.23 |
Mitochondria | -22.11* | 31.36* | 24.19 | 40.39* | -9.54 | 1.61 | 54.84* | 49.02* | 35.08* | -5.24 | -11.54 | 50.08* | 48.11* | 52.40* | -3.55 |
Mitotic Spindle | -10.93 | -1.74 | -12.65 | -5.64 | -3.55 | -5.28 | -12.24 | -12.05 | -8.27 | 1.28 | -12.10 | -12.34 | -17.42 | -8.89 | -0.39 |
None | -0.73 | -2.17 | 3.11 | 1.85 | 3.02 | -0.51 | -1.77 | 1.54 | 1.52 | 6.03 | -0.95 | -2.34 | 3.16 | 2.38 | 6.93 |
Nuclear Periphery | 2.31 | -3.64 | -3.51 | -4.67 | 2.98 | 0.19 | -8.43 | -0.02 | -0.25 | 9.26 | 1.56 | -9.72 | -2.68 | -3.91 | 8.30 |
Nuclear Periphery Foci | -1.02 | -5.37 | -2.96 | -2.63 | 4.35 | 1.75 | -3.80 | -1.13 | -4.70 | 3.41 | 0.45 | -7.38 | -3.20 | -3.41 | 2.40 |
Nucleolus | -1.89 | -3.94 | 2.19 | 2.61 | 4.26 | -1.41 | -3.65 | 1.01 | 2.26 | 6.60 | -2.31 | -5.89 | 1.85 | 3.43 | 8.14 |
Nucleus | 14.64 | -12.38 | -9.24 | -18.42 | 10.93 | -0.94 | -27.94 | -12.66 | -10.61 | 20.43 | 10.01 | -28.35 | -14.76 | -21.16 | 21.78 |
Peroxisomes | -7.12 | 2.53 | -9.36 | -2.83 | -8.33 | -1.32 | -0.84 | -10.09 | -8.11 | -8.86 | -6.16 | -1.72 | -13.83 | -7.55 | -9.94 |
Vacuole | 9.87 | -7.32 | -22.22 | -27.55 | -1.17 | -1.90 | -24.03 | -20.03 | -17.27 | 2.75 | 5.91 | -23.03 | -29.31 | -32.12 | -1.89 |
Vacuole Periphery | -4.52 | 4.11 | 8.71 | 10.06 | -0.38 | -2.23 | 13.30 | 12.89 | 11.41 | 0.53 | -4.42 | 15.50 | 15.44 | 15.08 | 0.30 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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