Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 71 | – | 141 | 123 | 145 | 111 | 216 | 162 | 130 | 146 | 137 | 167 | 51 | 146 | 124 | 37 | 7 | 20 | 112 | 45 | 244 | 129 | 173 | 224 |
Bud | 21 | – | 45 | 34 | 87 | 51 | 111 | 54 | 50 | 65 | 59 | 55 | 11 | 27 | 8 | 4 | 1 | 2 | 6 | 3 | 11 | 13 | 11 | 13 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 2 |
Bud Site | 3 | – | 1 | 6 | 9 | 5 | 6 | 6 | 1 | 6 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | – | 13 | 16 | 10 | 11 | 25 | 17 | 19 | 9 | 14 | 25 | 8 | 24 | 28 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 2 |
Cytoplasm | 15 | – | 20 | 20 | 18 | 13 | 18 | 11 | 9 | 6 | 15 | 6 | 4 | 9 | 10 | 22 | 1 | 5 | 1 | 1 | 4 | 0 | 5 | 0 |
Endoplasmic Reticulum | 8 | – | 9 | 5 | 5 | 4 | 17 | 17 | 30 | 20 | 21 | 21 | 3 | 15 | 16 | 5 | 0 | 3 | 1 | 0 | 0 | 2 | 4 | 1 |
Endosome | 10 | – | 11 | 5 | 29 | 20 | 24 | 13 | 9 | 11 | 9 | 13 | 10 | 5 | 7 | 1 | 0 | 1 | 3 | 0 | 2 | 6 | 9 | 4 |
Golgi | 18 | – | 67 | 60 | 49 | 38 | 69 | 112 | 119 | 73 | 58 | 102 | 38 | 174 | 171 | 44 | 8 | 25 | 9 | 4 | 12 | 18 | 16 | 19 |
Mitochondria | 44 | – | 44 | 85 | 95 | 184 | 301 | 157 | 174 | 191 | 163 | 219 | 17 | 10 | 4 | 12 | 4 | 7 | 1 | 1 | 0 | 5 | 3 | 1 |
Nucleus | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 1 | 5 | 14 | 10 | 14 | 4 | 6 | 9 | 9 | 11 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 6 | – | 61 | 64 | 80 | 75 | 77 | 83 | 35 | 50 | 18 | 53 | 11 | 14 | 12 | 2 | 0 | 0 | 2 | 0 | 12 | 3 | 3 | 4 |
SpindlePole | 1 | – | 10 | 13 | 13 | 9 | 9 | 13 | 5 | 11 | 4 | 5 | 2 | 4 | 2 | 5 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | – | 0 | 1 | 1 | 1 | 2 | 3 | 1 | 3 | 2 | 3 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 1 |
Unique Cell Count | 119 | 244 | 241 | 312 | 327 | 550 | 381 | 347 | 366 | 329 | 428 | 80 | 248 | 242 | 101 | 16 | 46 | 142 | 59 | 293 | 192 | 244 | 280 | |
Labelled Cell Count | 202 | 424 | 438 | 555 | 532 | 891 | 653 | 589 | 601 | 515 | 688 | 157 | 430 | 383 | 141 | 22 | 69 | 142 | 59 | 293 | 192 | 244 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.5 | – | 22.9 | 18.9 | 19.7 | 20.9 | 19.6 | 22.4 | 23.2 | 20.9 | 21.9 | 21.6 | 22.5 | 23.0 | 24.4 | 32.9 | 34.7 | 33.6 | 24.0 | 22.1 | 23.6 |
Std Deviation (1e-4) | 8.8 | – | 11.6 | 10.8 | 10.4 | 12.0 | 11.1 | 12.8 | 14.0 | 12.3 | 13.5 | 14.4 | 12.8 | 11.3 | 11.6 | 18.2 | 17.5 | 12.3 | 12.4 | 10.7 | 10.3 |
Intensity Change (Log2) | – | – | – | -0.28 | -0.22 | -0.13 | -0.23 | -0.03 | 0.02 | -0.13 | -0.06 | -0.09 | -0.03 | 0.01 | 0.09 | 0.52 | 0.6 | 0.55 | 0.07 | -0.05 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | -2.6 | -5.7 | -4.8 | -3.7 | -4.9 | -4.3 | -3.8 | -4.7 | 0.9 | 0.2 | -1.4 | -3.6 | -1.1 | -1.8 |
Bud | -1.3 | 2.6 | -0.9 | 0.6 | -1.4 | -1.3 | -0.2 | -0.2 | -2.0 | -1.0 | -2.4 | -5.4 | -3.5 | 0 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.6 | -1.2 | -1.2 | -0.5 | -0.5 | 0.1 | -1.9 | -0.6 | 0.3 | 1.5 | 1.8 | 2.5 | 0 | 0 | 0 |
Cytoplasm | 0 | -1.1 | -2.1 | -3.0 | -3.0 | -3.1 | -3.9 | -1.8 | -4.4 | -0.9 | -2.2 | -1.9 | 3.5 | 0 | 0 |
Endoplasmic Reticulum | -1.1 | -1.6 | -2.0 | -0.4 | 0.5 | 2.4 | 1.0 | 1.4 | 0.7 | 0 | 1.2 | 1.5 | 0 | 0 | 0 |
Endosome | -1.5 | 2.2 | 0.8 | -0.1 | -0.7 | -1.3 | -1.0 | -1.1 | -1.0 | 2.5 | -1.6 | -0.9 | 0 | 0 | 0 |
Golgi | -0.6 | -3.4 | -4.8 | -5.1 | 0.5 | 1.8 | -2.2 | -2.8 | -1.0 | 3.3 | 9.5 | 9.5 | 2.9 | 0 | 3.6 |
Mitochondria | 4.3 | 3.4 | 9.2 | 9.6 | 6.1 | 8.0 | 8.5 | 7.8 | 8.5 | 0.6 | -5.0 | -6.1 | -1.4 | 0 | -0.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.4 | 0.2 | -0.6 | -3.8 | -0.9 | -4.8 | -3.6 | -6.7 | -4.2 | -2.1 | -6.0 | -6.2 | -5.0 | 0 | -3.8 |
SpindlePole | 0.7 | 0 | -0.9 | -2.1 | -0.4 | -2.0 | -0.7 | -2.2 | -2.5 | 0 | -1.7 | -2.3 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.331 | 15.6772 | 13.1406 | 8.8616 | 12.8136 | 14.8597 | 14.9453 | 19.2954 | 18.6879 | 16.0004 | 17.6392 | 19.3751 | 9.8207 | 10.9428 | 11.3192 | 11.7204 | 9.2811 | 11.473 |
Actin | 0.6639 | 0.8586 | 0.8484 | 0.9724 | 0.682 | 0.9357 | 0.7484 | 0.7902 | 0.8498 | 0.7608 | 0.8256 | 0.9125 | 0.7547 | 0.8861 | 0.893 | 0.7091 | 0.8241 | 0.9156 |
Bud | 0.0011 | 0.0045 | 0.0051 | 0.0055 | 0.0036 | 0.0005 | 0.0021 | 0.003 | 0.0136 | 0.0059 | 0.0038 | 0.0002 | 0.002 | 0.0034 | 0.017 | 0.0035 | 0.0005 | 0.0003 |
Bud Neck | 0.0022 | 0.0009 | 0.002 | 0.0002 | 0.0005 | 0.0075 | 0.0021 | 0.0032 | 0.0044 | 0.01 | 0.0041 | 0.0185 | 0.0036 | 0.001 | 0.0013 | 0.0014 | 0.0011 | 0.0118 |
Bud Periphery | 0.0009 | 0.0024 | 0.0077 | 0.0043 | 0.0222 | 0.0007 | 0.0023 | 0.002 | 0.0088 | 0.0087 | 0.0056 | 0.0003 | 0.0017 | 0.0022 | 0.0207 | 0.0041 | 0.0004 | 0.0004 |
Bud Site | 0.0209 | 0.033 | 0.0163 | 0.0133 | 0.0015 | 0.0011 | 0.0067 | 0.0692 | 0.021 | 0.0078 | 0.0101 | 0.0003 | 0.0197 | 0.0593 | 0.0132 | 0.0052 | 0.0008 | 0.0006 |
Cell Periphery | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0011 | 0.0001 | 0.0005 | 0.0007 | 0.0004 | 0.0017 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.0187 | 0.016 | 0.0064 | 0.0002 | 0.0008 | 0.0016 | 0.006 | 0.0018 | 0.0072 | 0.0037 | 0.0026 | 0.0001 | 0.0304 | 0.0024 | 0.0122 | 0.0023 | 0.0023 | 0.0004 |
Cytoplasmic Foci | 0.0592 | 0.0251 | 0.0187 | 0.0006 | 0.0225 | 0.0046 | 0.0223 | 0.0335 | 0.0089 | 0.0486 | 0.0318 | 0.0011 | 0.0305 | 0.0111 | 0.0093 | 0.0202 | 0.0194 | 0.0183 |
Eisosomes | 0.0054 | 0.0068 | 0.0188 | 0.0004 | 0.1022 | 0.0012 | 0.0323 | 0.0534 | 0.0179 | 0.0013 | 0.0007 | 0.0399 | 0.0017 | 0.0003 | 0.0009 | 0.0291 | 0.0005 | 0.0019 |
Endoplasmic Reticulum | 0.0019 | 0 | 0.003 | 0 | 0.0024 | 0.0017 | 0.0077 | 0.0002 | 0.0009 | 0.0008 | 0.0056 | 0 | 0.0141 | 0.0001 | 0.0001 | 0.0025 | 0.0023 | 0.0005 |
Endosome | 0.0423 | 0.0004 | 0.0118 | 0 | 0.0508 | 0.0106 | 0.0575 | 0.0016 | 0.0148 | 0.0456 | 0.0343 | 0.0008 | 0.043 | 0.0043 | 0.0018 | 0.0781 | 0.0475 | 0.0056 |
Golgi | 0.0392 | 0.0039 | 0.0122 | 0.0009 | 0.0618 | 0.0082 | 0.0335 | 0.0062 | 0.024 | 0.0465 | 0.0194 | 0.0203 | 0.0233 | 0.0185 | 0.0158 | 0.0608 | 0.0669 | 0.0141 |
Lipid Particles | 0.0198 | 0.0004 | 0.0025 | 0 | 0.0309 | 0.0035 | 0.0174 | 0.0007 | 0.0044 | 0.0196 | 0.0245 | 0.0003 | 0.0073 | 0.0005 | 0.0002 | 0.0306 | 0.0199 | 0.0062 |
Mitochondria | 0.0165 | 0.0008 | 0.0181 | 0.0005 | 0.0048 | 0.0019 | 0.0125 | 0.0063 | 0.0136 | 0.0125 | 0.0075 | 0.0005 | 0.0054 | 0.0006 | 0.0008 | 0.0077 | 0.0063 | 0.0069 |
None | 0.0304 | 0.0011 | 0.0063 | 0 | 0.0005 | 0.0002 | 0.0078 | 0.0001 | 0.0003 | 0.0001 | 0.0021 | 0 | 0.0032 | 0 | 0.0044 | 0.0004 | 0.0005 | 0.0005 |
Nuclear Periphery | 0.0037 | 0 | 0.0039 | 0 | 0.0018 | 0.0076 | 0.0148 | 0.0001 | 0.0004 | 0.0007 | 0.0071 | 0 | 0.0238 | 0 | 0.0001 | 0.0036 | 0.0012 | 0.0089 |
Nucleolus | 0.0109 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0006 | 0 | 0.0018 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 |
Nucleus | 0.0115 | 0.0001 | 0.0035 | 0 | 0.0002 | 0.0007 | 0.0022 | 0.0001 | 0.0002 | 0.0007 | 0.0015 | 0 | 0.0103 | 0 | 0.0005 | 0.0006 | 0.0001 | 0.0005 |
Peroxisomes | 0.0388 | 0.045 | 0.0066 | 0.0012 | 0.0072 | 0.0099 | 0.0062 | 0.0272 | 0.0064 | 0.0142 | 0.0052 | 0.005 | 0.0134 | 0.0098 | 0.0084 | 0.0273 | 0.0028 | 0.0048 |
Punctate Nuclear | 0.0092 | 0.0003 | 0.0055 | 0 | 0.0018 | 0.0013 | 0.0057 | 0.0002 | 0.0005 | 0.001 | 0.0025 | 0 | 0.0028 | 0.0001 | 0.0001 | 0.0008 | 0.0006 | 0.0006 |
Vacuole | 0.0023 | 0.0001 | 0.001 | 0 | 0.0005 | 0.0004 | 0.0084 | 0.0001 | 0.0016 | 0.0076 | 0.0031 | 0 | 0.0044 | 0.0001 | 0.0001 | 0.0075 | 0.0011 | 0.0003 |
Vacuole Periphery | 0.001 | 0 | 0.0008 | 0 | 0.0007 | 0.0007 | 0.003 | 0 | 0.0006 | 0.0016 | 0.0019 | 0 | 0.0023 | 0.0001 | 0 | 0.0048 | 0.0015 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.3095 | 78.5685 | 96.9574 | 109.9455 | 98.4707 | 104.0732 | 104.9133 | 104.0286 | 114.08 | 109.9096 |
Translational Efficiency | 1.2324 | 1.2318 | 1.1019 | 1.0311 | 1.1 | 1.4452 | 1.1841 | 1.1198 | 1.1536 | 1.0291 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1498 | 857 | 211 | 227 | 1608 | 1739 | 398 | 190 | 3106 | 2596 | 609 | 417 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1300.54 | 1841.10 | 1812.10 | 2083.73 | 1474.53 | 1590.40 | 1967.44 | 2280.34 | 1390.62 | 1673.16 | 1913.62 | 2173.31 |
Standard Deviation | 451.60 | 667.34 | 702.45 | 812.57 | 536.89 | 603.15 | 737.73 | 825.49 | 505.12 | 636.09 | 729.45 | 824.32 |
Intensity Change Log 2 | — | 0.501457 | 0.478552 | 0.680058 | — | 0.109134 | 0.416064 | 0.628994 | — | 0.306315 | 0.445686 | 0.653150 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.806854* | 0.840153* | 0.690633* | 0.656051* | 0.693140* | 0.794247* | 0.607330* | 0.718769* | 0.747984* | 0.809402* | 0.636192* | 0.684627* |
Bud Neck | 0.005939 | 0.005684 | 0.020682 | 0.022804 | 0.013779 | 0.005493 | 0.020667 | 0.017445 | 0.009998 | 0.005556 | 0.020672 | 0.020362 |
Bud Site | 0.059124 | 0.034923 | 0.122337* | 0.077423 | 0.141395* | 0.042719 | 0.149980* | 0.056912 | 0.101716* | 0.040145 | 0.140403* | 0.068077 |
Cell Periphery | 0.000887 | 0.000818 | 0.001225 | 0.002357 | 0.018782 | 0.000736 | 0.001885 | 0.004623 | 0.010151 | 0.000763 | 0.001657 | 0.003389 |
Cytoplasm | 0.006833 | 0.002908 | 0.016652 | 0.011111 | 0.014665 | 0.005034 | 0.023384 | 0.001746 | 0.010888 | 0.004332 | 0.021052 | 0.006844 |
Cytoplasmic Foci | 0.017698 | 0.008794 | 0.030161 | 0.045989 | 0.015094 | 0.020632 | 0.056000 | 0.016867 | 0.016350 | 0.016724 | 0.047048 | 0.032720 |
Eisosomes | 0.006768 | 0.045710 | 0.018717 | 0.010002 | 0.015364 | 0.006926 | 0.006937 | 0.024200 | 0.011218 | 0.019730 | 0.011018 | 0.016471 |
Endoplasmic Reticulum | 0.000788 | 0.001570 | 0.001970 | 0.006407 | 0.009407 | 0.001536 | 0.002184 | 0.010292 | 0.005250 | 0.001547 | 0.002110 | 0.008177 |
Endosome | 0.016397 | 0.013346 | 0.033215 | 0.054138 | 0.016332 | 0.024615 | 0.036534 | 0.049685 | 0.016364 | 0.020895 | 0.035384 | 0.052109 |
Golgi | 0.017618 | 0.012127 | 0.014339 | 0.050411 | 0.015198 | 0.028293 | 0.025144 | 0.048406 | 0.016365 | 0.022956 | 0.021401 | 0.049498 |
Lipid Particles | 0.014353 | 0.007249 | 0.014347 | 0.038698 | 0.011046 | 0.033256 | 0.023301 | 0.032662 | 0.012641 | 0.024670 | 0.020198 | 0.035947 |
Mitochondria | 0.004607 | 0.004962 | 0.007140 | 0.001696 | 0.013750 | 0.004169 | 0.002730 | 0.001159 | 0.009340 | 0.004431 | 0.004258 | 0.001451 |
Mitotic Spindle | 0.000673 | 0.000428 | 0.002779 | 0.001703 | 0.000592 | 0.000938 | 0.003951 | 0.000613 | 0.000631 | 0.000770 | 0.003545 | 0.001207 |
None | 0.000095 | 0.000079 | 0.000056 | 0.000038 | 0.000249 | 0.000112 | 0.000048 | 0.000047 | 0.000175 | 0.000102 | 0.000051 | 0.000042 |
Nuclear Periphery | 0.000012 | 0.000066 | 0.000077 | 0.000181 | 0.000120 | 0.000025 | 0.000093 | 0.000111 | 0.000068 | 0.000039 | 0.000087 | 0.000149 |
Nuclear Periphery Foci | 0.000051 | 0.000427 | 0.000294 | 0.000713 | 0.000243 | 0.000121 | 0.000506 | 0.000288 | 0.000150 | 0.000222 | 0.000433 | 0.000519 |
Nucleolus | 0.000011 | 0.000011 | 0.000023 | 0.000016 | 0.000046 | 0.000028 | 0.000018 | 0.000008 | 0.000029 | 0.000022 | 0.000020 | 0.000012 |
Nucleus | 0.000091 | 0.000073 | 0.000171 | 0.000286 | 0.000317 | 0.000100 | 0.000282 | 0.000091 | 0.000208 | 0.000091 | 0.000244 | 0.000197 |
Peroxisomes | 0.040643 | 0.019398 | 0.012004 | 0.010720 | 0.016345 | 0.029930 | 0.025424 | 0.009815 | 0.028064 | 0.026453 | 0.020775 | 0.010307 |
Vacuole | 0.000437 | 0.000892 | 0.012849 | 0.008433 | 0.002457 | 0.000875 | 0.013239 | 0.005634 | 0.001483 | 0.000881 | 0.013104 | 0.007158 |
Vacuole Periphery | 0.000121 | 0.000379 | 0.000327 | 0.000825 | 0.001678 | 0.000216 | 0.000363 | 0.000625 | 0.000927 | 0.000270 | 0.000351 | 0.000734 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.95 | 4.82 | 6.33 | 7.52 | 1.11 | -8.68 | 4.57 | -0.96 | 3.22 | -4.05 | -7.47 | 7.43 | 3.85 | 7.33 | -2.32 |
Bud Neck | 0.26 | -4.81 | -5.72 | -5.77 | -0.57 | 7.88 | -2.86 | -1.37 | -4.62 | 0.98 | 6.31 | -5.65 | -5.13 | -7.42 | 0.10 |
Bud Site | 4.48 | -4.21 | -1.71 | -3.94 | 2.55 | 14.06* | -0.88 | 7.44 | -1.58 | 6.39 | 13.57 | -4.14 | 4.08 | -3.88 | 6.40 |
Cell Periphery | 0.43 | -1.70 | -2.62 | -2.74 | -1.91 | 9.17 | 8.35 | 5.94 | -2.86 | -1.88 | 9.08 | 7.85 | 5.44 | -3.81 | -2.26 |
Cytoplasm | 3.55 | -1.94 | -1.29 | -2.09 | 0.56 | 5.34 | -2.13 | 6.90 | 2.97 | 4.58 | 5.97 | -3.08 | 0.97 | -1.34 | 3.19 |
Cytoplasmic Foci | 3.94 | -2.28 | -3.36 | -4.29 | -1.68 | -2.58 | -6.45 | -0.48 | 0.80 | 5.32 | -0.37 | -6.71 | -3.31 | -3.19 | 1.97 |
Eisosomes | -7.31 | -2.45 | -0.97 | 6.01 | 1.58 | 3.97 | 3.48 | -1.21 | -2.43 | -2.41 | -3.86 | 0.16 | -1.37 | 0.84 | -1.35 |
Endoplasmic Reticulum | -1.81 | -3.32 | -3.50 | -2.91 | -2.70 | 5.92 | 5.36 | -0.34 | -4.35 | -4.01 | 5.15 | 4.33 | -2.00 | -5.16 | -4.71 |
Endosome | 1.28 | -2.67 | -5.12 | -5.46 | -2.17 | -3.65 | -4.93 | -4.80 | -3.58 | -1.74 | -2.70 | -5.52 | -7.00 | -6.03 | -2.81 |
Golgi | 2.20 | 0.96 | -3.86 | -4.49 | -4.06 | -5.38 | -2.19 | -4.42 | -2.65 | -2.83 | -3.63 | -1.53 | -5.77 | -4.56 | -4.50 |
Lipid Particles | 3.04 | -0.02 | -3.43 | -4.49 | -3.06 | -6.85 | -2.71 | -3.20 | 0.06 | -1.18 | -5.19 | -2.28 | -4.73 | -2.20 | -2.74 |
Mitochondria | -0.29 | -1.02 | 3.65 | 2.84 | 2.23 | 5.74 | 6.64 | 8.19 | 3.71 | 1.88 | 4.75 | 4.00 | 8.85 | 4.44 | 2.76 |
Mitotic Spindle | 1.64 | -2.02 | -1.73 | -2.08 | 0.76 | -1.99 | -4.85 | -0.14 | 1.30 | 4.68 | -1.12 | -5.03 | -1.73 | -1.33 | 3.30 |
None | 0.35 | 1.51 | 2.21 | 1.37 | 1.18 | 2.64 | 4.96 | 5.06 | 1.95 | 0.27 | 2.14 | 5.06 | 5.49 | 2.38 | 0.92 |
Nuclear Periphery | -1.10 | -4.28 | -3.83 | -1.88 | -2.32 | 1.38 | 0.35 | 0.13 | -3.45 | -0.51 | 0.75 | -0.56 | -1.93 | -3.60 | -2.15 |
Nuclear Periphery Foci | -0.99 | -2.86 | -2.05 | -0.63 | -1.27 | 0.99 | -1.99 | -0.38 | -2.06 | 1.94 | -0.52 | -3.19 | -1.98 | -1.41 | -0.29 |
Nucleolus | -0.12 | -2.09 | -1.37 | -1.22 | 0.46 | 1.54 | 3.79 | 5.30 | 2.02 | 2.56 | 0.87 | 1.88 | 2.74 | 1.04 | 1.17 |
Nucleus | 0.25 | -1.42 | -1.64 | -1.67 | -1.11 | 3.31 | 0.13 | 3.78 | 0.36 | 3.23 | 2.83 | -1.04 | -0.62 | -1.56 | -0.13 |
Peroxisomes | 5.45 | 8.00 | 8.88 | 2.58 | 0.43 | -4.63 | -2.22 | 1.19 | 3.51 | 2.43 | 0.66 | 2.49 | 5.77 | 5.09 | 2.88 |
Vacuole | -1.13 | -2.38 | -3.51 | -3.28 | 0.68 | 3.87 | -3.88 | -1.99 | -3.00 | 2.53 | 2.34 | -4.60 | -3.94 | -4.35 | 2.11 |
Vacuole Periphery | -1.26 | -2.23 | -3.15 | -1.49 | -2.06 | 4.79 | 4.21 | 3.00 | -2.38 | -1.42 | 3.79 | 3.41 | 0.86 | -2.91 | -2.48 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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