Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.6 0.58 0.51 0.46 0.34 0.39 0.43 0.37 0.4 0.42 0.39 0.64 0.59 0.51 0.37 0.44 0.43 0.79 0.77 0.84 0.67 0.71 0.8
Bud 0.18 0.18 0.14 0.28 0.16 0.2 0.14 0.14 0.18 0.18 0.13 0.14 0.11 0 0 0.06 0 0 0.05 0 0.07 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.05 0.07 0 0 0.05 0 0.05 0 0 0.06 0.1 0.1 0.12 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.08 0.08 0.06 0 0 0 0 0 0.05 0 0.05 0 0 0.22 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0.09 0.05 0.06 0.05 0 0.06 0.07 0.05 0 0.07 0 0 0 0 0 0
Endosome 0.08 0.05 0 0.09 0.06 0 0 0 0 0 0 0.12 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.15 0.27 0.25 0.16 0.12 0.13 0.29 0.34 0.2 0.18 0.24 0.47 0.7 0.71 0.44 0.5 0.54 0.07 0.08 0 0.1 0.07 0.07
Mitochondria 0.37 0.18 0.35 0.3 0.56 0.55 0.41 0.5 0.52 0.5 0.51 0.21 0 0 0.12 0.25 0.15 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0.25 0.27 0.26 0.23 0.14 0.22 0.1 0.14 0.05 0.12 0.14 0.06 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 71 141 123 145 111 216 162 130 146 137 167 51 146 124 37 7 20 112 45 244 129 173 224
Bud 21 45 34 87 51 111 54 50 65 59 55 11 27 8 4 1 2 6 3 11 13 11 13
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1 5 2
Bud Site 3 1 6 9 5 6 6 1 6 5 7 0 0 0 1 0 1
Cell Periphery 4 13 16 10 11 25 17 19 9 14 25 8 24 28 4 0 2 0 0 0 1 1 2
Cytoplasm 15 20 20 18 13 18 11 9 6 15 6 4 9 10 22 1 5 1 1 4 0 5 0
Endoplasmic Reticulum 8 9 5 5 4 17 17 30 20 21 21 3 15 16 5 0 3 1 0 0 2 4 1
Endosome 10 11 5 29 20 24 13 9 11 9 13 10 5 7 1 0 1 3 0 2 6 9 4
Golgi 18 67 60 49 38 69 112 119 73 58 102 38 174 171 44 8 25 9 4 12 18 16 19
Mitochondria 44 44 85 95 184 301 157 174 191 163 219 17 10 4 12 4 7 1 1 0 5 3 1
Nucleus 1 1 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 1 0 0 2 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 5 14 10 14 4 6 9 9 11 1 1 0 2 0 0 0 0 0 0 0 1
Peroxisomes 6 61 64 80 75 77 83 35 50 18 53 11 14 12 2 0 0 2 0 12 3 3 4
SpindlePole 1 10 13 13 9 9 13 5 11 4 5 2 4 2 5 1 2 1 0 1 1 1 0
Vac/Vac Membrane 0 0 1 1 1 2 3 1 3 2 3 1 1 1 2 0 1 0 0 0 3 2 1
Unique Cell Count 119 244 241 312 327 550 381 347 366 329 428 80 248 242 101 16 46 142 59 293 192 244 280
Labelled Cell Count 202 424 438 555 532 891 653 589 601 515 688 157 430 383 141 22 69 142 59 293 192 244 280


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.5 22.9 18.9 19.7 20.9 19.6 22.4 23.2 20.9 21.9 21.6 22.5 23.0 24.4 32.9 34.7 33.6 24.0 22.1 23.6
Std Deviation (1e-4) 8.8 11.6 10.8 10.4 12.0 11.1 12.8 14.0 12.3 13.5 14.4 12.8 11.3 11.6 18.2 17.5 12.3 12.4 10.7 10.3
Intensity Change (Log2) -0.28 -0.22 -0.13 -0.23 -0.03 0.02 -0.13 -0.06 -0.09 -0.03 0.01 0.09 0.52 0.6 0.55 0.07 -0.05 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -2.6 -5.7 -4.8 -3.7 -4.9 -4.3 -3.8 -4.7 0.9 0.2 -1.4 -3.6 -1.1 -1.8
Bud -1.3 2.6 -0.9 0.6 -1.4 -1.3 -0.2 -0.2 -2.0 -1.0 -2.4 -5.4 -3.5 0 -2.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.6 -1.2 -1.2 -0.5 -0.5 0.1 -1.9 -0.6 0.3 1.5 1.8 2.5 0 0 0
Cytoplasm 0 -1.1 -2.1 -3.0 -3.0 -3.1 -3.9 -1.8 -4.4 -0.9 -2.2 -1.9 3.5 0 0
Endoplasmic Reticulum -1.1 -1.6 -2.0 -0.4 0.5 2.4 1.0 1.4 0.7 0 1.2 1.5 0 0 0
Endosome -1.5 2.2 0.8 -0.1 -0.7 -1.3 -1.0 -1.1 -1.0 2.5 -1.6 -0.9 0 0 0
Golgi -0.6 -3.4 -4.8 -5.1 0.5 1.8 -2.2 -2.8 -1.0 3.3 9.5 9.5 2.9 0 3.6
Mitochondria 4.3 3.4 9.2 9.6 6.1 8.0 8.5 7.8 8.5 0.6 -5.0 -6.1 -1.4 0 -0.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.4 0.2 -0.6 -3.8 -0.9 -4.8 -3.6 -6.7 -4.2 -2.1 -6.0 -6.2 -5.0 0 -3.8
SpindlePole 0.7 0 -0.9 -2.1 -0.4 -2.0 -0.7 -2.2 -2.5 0 -1.7 -2.3 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.331 15.6772 13.1406 8.8616 12.8136 14.8597 14.9453 19.2954 18.6879 16.0004 17.6392 19.3751 9.8207 10.9428 11.3192 11.7204 9.2811 11.473
Actin 0.6639 0.8586 0.8484 0.9724 0.682 0.9357 0.7484 0.7902 0.8498 0.7608 0.8256 0.9125 0.7547 0.8861 0.893 0.7091 0.8241 0.9156
Bud 0.0011 0.0045 0.0051 0.0055 0.0036 0.0005 0.0021 0.003 0.0136 0.0059 0.0038 0.0002 0.002 0.0034 0.017 0.0035 0.0005 0.0003
Bud Neck 0.0022 0.0009 0.002 0.0002 0.0005 0.0075 0.0021 0.0032 0.0044 0.01 0.0041 0.0185 0.0036 0.001 0.0013 0.0014 0.0011 0.0118
Bud Periphery 0.0009 0.0024 0.0077 0.0043 0.0222 0.0007 0.0023 0.002 0.0088 0.0087 0.0056 0.0003 0.0017 0.0022 0.0207 0.0041 0.0004 0.0004
Bud Site 0.0209 0.033 0.0163 0.0133 0.0015 0.0011 0.0067 0.0692 0.021 0.0078 0.0101 0.0003 0.0197 0.0593 0.0132 0.0052 0.0008 0.0006
Cell Periphery 0.0003 0.0004 0.0005 0.0001 0.0011 0.0001 0.0005 0.0007 0.0004 0.0017 0.0003 0.0003 0.0005 0.0002 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.0187 0.016 0.0064 0.0002 0.0008 0.0016 0.006 0.0018 0.0072 0.0037 0.0026 0.0001 0.0304 0.0024 0.0122 0.0023 0.0023 0.0004
Cytoplasmic Foci 0.0592 0.0251 0.0187 0.0006 0.0225 0.0046 0.0223 0.0335 0.0089 0.0486 0.0318 0.0011 0.0305 0.0111 0.0093 0.0202 0.0194 0.0183
Eisosomes 0.0054 0.0068 0.0188 0.0004 0.1022 0.0012 0.0323 0.0534 0.0179 0.0013 0.0007 0.0399 0.0017 0.0003 0.0009 0.0291 0.0005 0.0019
Endoplasmic Reticulum 0.0019 0 0.003 0 0.0024 0.0017 0.0077 0.0002 0.0009 0.0008 0.0056 0 0.0141 0.0001 0.0001 0.0025 0.0023 0.0005
Endosome 0.0423 0.0004 0.0118 0 0.0508 0.0106 0.0575 0.0016 0.0148 0.0456 0.0343 0.0008 0.043 0.0043 0.0018 0.0781 0.0475 0.0056
Golgi 0.0392 0.0039 0.0122 0.0009 0.0618 0.0082 0.0335 0.0062 0.024 0.0465 0.0194 0.0203 0.0233 0.0185 0.0158 0.0608 0.0669 0.0141
Lipid Particles 0.0198 0.0004 0.0025 0 0.0309 0.0035 0.0174 0.0007 0.0044 0.0196 0.0245 0.0003 0.0073 0.0005 0.0002 0.0306 0.0199 0.0062
Mitochondria 0.0165 0.0008 0.0181 0.0005 0.0048 0.0019 0.0125 0.0063 0.0136 0.0125 0.0075 0.0005 0.0054 0.0006 0.0008 0.0077 0.0063 0.0069
None 0.0304 0.0011 0.0063 0 0.0005 0.0002 0.0078 0.0001 0.0003 0.0001 0.0021 0 0.0032 0 0.0044 0.0004 0.0005 0.0005
Nuclear Periphery 0.0037 0 0.0039 0 0.0018 0.0076 0.0148 0.0001 0.0004 0.0007 0.0071 0 0.0238 0 0.0001 0.0036 0.0012 0.0089
Nucleolus 0.0109 0.0002 0.0008 0 0.0001 0.0001 0.0007 0 0.0001 0.0005 0.0006 0 0.0018 0 0 0.0003 0.0001 0.0003
Nucleus 0.0115 0.0001 0.0035 0 0.0002 0.0007 0.0022 0.0001 0.0002 0.0007 0.0015 0 0.0103 0 0.0005 0.0006 0.0001 0.0005
Peroxisomes 0.0388 0.045 0.0066 0.0012 0.0072 0.0099 0.0062 0.0272 0.0064 0.0142 0.0052 0.005 0.0134 0.0098 0.0084 0.0273 0.0028 0.0048
Punctate Nuclear 0.0092 0.0003 0.0055 0 0.0018 0.0013 0.0057 0.0002 0.0005 0.001 0.0025 0 0.0028 0.0001 0.0001 0.0008 0.0006 0.0006
Vacuole 0.0023 0.0001 0.001 0 0.0005 0.0004 0.0084 0.0001 0.0016 0.0076 0.0031 0 0.0044 0.0001 0.0001 0.0075 0.0011 0.0003
Vacuole Periphery 0.001 0 0.0008 0 0.0007 0.0007 0.003 0 0.0006 0.0016 0.0019 0 0.0023 0.0001 0 0.0048 0.0015 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 100.3095 78.5685 96.9574 109.9455 98.4707 104.0732 104.9133 104.0286 114.08 109.9096
Translational Efficiency 1.2324 1.2318 1.1019 1.0311 1.1 1.4452 1.1841 1.1198 1.1536 1.0291

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
Localization
Cell Percentages actin (76%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arc40

Arc40


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arc40-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available