Standard name
Human Ortholog
Description RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.08 0.08 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0.07 0.11 0.12 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.06 0.06 0.06 0.27 0.25 0.24 0.18 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.92 0.95 0.94 0.93 0.97 0.91 0.93 0.92 0.9 0.86 0.9 0.89 0.94 0.91 0.87 0.81 0.89 0.83 0.87 0.92 0.89 0.86 0.79 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0 0.1 0.13 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1
Bud 0 1 0 0 1 1 5 2 1 4 4 3 0 0 1 0 0 0 3 3 2 1 3 9
Bud Neck 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 1 6 4 11 10 39 38 30 1 0 0 1 0 0
Cell Periphery 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 8 5 16 16 15 21 27 11 8 13 8 12 11 32 60 42 14 16 0 0 3 0 0 0
Endoplasmic Reticulum 0 1 2 1 0 0 1 1 0 1 0 0 0 1 0 7 3 3 0 0 1 0 1 2
Endosome 0 2 1 0 0 2 0 2 0 1 0 0 1 3 1 1 1 0 2 5 2 0 2 4
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 1 3 2 1 0 3 2
Mitochondria 9 13 3 4 4 29 39 30 103 117 109 96 12 6 6 9 3 8 1 4 4 0 2 8
Nucleus 158 294 305 280 503 418 603 480 341 401 414 474 337 410 484 290 194 122 154 286 292 132 299 310
Nuclear Periphery 1 1 0 0 3 20 5 12 4 15 9 14 7 9 3 2 2 0 0 0 0 0 0 2
Nucleolus 2 0 0 1 0 5 9 11 9 42 20 23 4 4 7 2 1 2 1 0 0 1 8 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 1 3 3 2 2 4 1 3 4 5 1 1 3 3 3 2 1 0 0 0 2 3
Vac/Vac Membrane 2 4 1 0 3 8 2 6 1 5 3 8 1 3 5 24 5 11 4 6 14 16 49 74
Unique Cell Count 171 308 326 300 520 458 648 520 380 465 460 535 357 449 554 357 217 147 177 312 329 154 378 449
Labelled Cell Count 180 324 329 306 536 513 699 571 478 641 610 666 376 470 571 383 226 165 177 312 329 154 378 449


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 6.2 6.9 6.3 6.6 7.5 6.2 6.1 5.1 5.3 5.0 5.9 6.8 6.3 6.4 9.2 9.9 9.2 6.0 5.8 6.4
Std Deviation (1e-4) 1.3 1.1 1.3 1.6 1.4 12.9 4.2 4.5 4.5 4.5 3.5 7.8 4.4 1.3 1.6 2.4 2.2 2.3 1.7 1.2 1.5
Intensity Change (Log2) -0.13 -0.06 0.1 -0.16 -0.18 -0.44 -0.38 -0.48 -0.22 -0.03 -0.14 -0.11 0.41 0.52 0.41 -0.22 -0.26 -0.12

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF100AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 5.4 5.3 4.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -1.5 -0.2 -0.5 -2.2 -2.0 -1.6 -2.5 -2.1 -1.2 1.3 3.0 3.2 0.8 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 3.7 3.5 9.7 9.4 9.0 7.6 2.2 0 0 1.6 0 0
Nucleus -0.1 2.2 -1.2 -0.3 -0.7 -1.8 -3.3 -1.8 -2.4 0.5 -1.2 -2.9 -4.8 -1.7 -3.6
Nuclear Periphery 0 0 3.8 0 0 0 3.3 0 2.9 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 5.6 3.8 3.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin
Bud
Bud Neck
Bud Periphery
Bud Site
Cell Periphery
Cytoplasm
Cytoplasmic Foci
Eisosomes
Endoplasmic Reticulum
Endosome
Golgi
Lipid Particles
Mitochondria
None
Nuclear Periphery
Nucleolus
Nucleus
Peroxisomes
Punctate Nuclear
Vacuole
Vacuole Periphery

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9233 9.2835 5.7407 10.1269 6.7323 9.0818 7.5288 7.5232 11.2299 10.5362
Translational Efficiency 0.6825 0.4639 0.729 0.4996 0.7038 0.5712 0.6257 0.5889 0.4131 0.559

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1406 1719 579 1740 935 2137 99 1857 2341 3856 678 3597

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.00 799.34 1028.86 982.23 721.11 887.22 1048.09 957.45 705.43 848.04 1031.67 969.44
Standard Deviation 82.55 144.73 129.63 161.14 96.69 124.96 179.77 145.66 89.39 141.07 138.25 153.84
Intensity Change Log 2 0.201796 0.565962 0.499048 0.299073 0.539471 0.408978 0.252150 0.552533 0.453885

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000119 0.000415 0.000951 0.000756 0.000451 0.000341 0.001136 0.000572 0.000252 0.000374 0.000978 0.000661
Bud Neck 0.001977 0.007427 0.017486 0.023149 0.002572 0.008116 0.008981 0.024243 0.002214 0.007809 0.016244 0.023714
Bud Site 0.000360 0.001325 0.001289 0.004175 0.000470 0.001641 0.001138 0.001943 0.000404 0.001500 0.001267 0.003022
Cell Periphery 0.000106 0.000244 0.000051 0.000117 0.000120 0.000134 0.000062 0.000094 0.000111 0.000183 0.000053 0.000105
Cytoplasm 0.005227 0.004980 0.000424 0.007486 0.004066 0.001451 0.000854 0.002523 0.004763 0.003024 0.000487 0.004924
Cytoplasmic Foci 0.000893 0.000504 0.000018 0.000856 0.000555 0.000982 0.000067 0.000014 0.000758 0.000769 0.000025 0.000421
Eisosomes 0.000012 0.000020 0.000038 0.000025 0.000021 0.000025 0.000044 0.000027 0.000016 0.000023 0.000039 0.000026
Endoplasmic Reticulum 0.002024 0.005667 0.005544 0.005102 0.005008 0.010226 0.007146 0.004898 0.003216 0.008193 0.005778 0.004997
Endosome 0.000148 0.000373 0.000148 0.001615 0.000235 0.000890 0.000197 0.000173 0.000182 0.000659 0.000155 0.000871
Golgi 0.000094 0.000161 0.000061 0.001133 0.000164 0.000574 0.000065 0.000047 0.000122 0.000390 0.000062 0.000572
Lipid Particles 0.000212 0.000149 0.000074 0.001502 0.000263 0.000568 0.000119 0.000038 0.000232 0.000381 0.000080 0.000746
Mitochondria 0.001050 0.001020 0.001492 0.003260 0.001490 0.004188 0.001167 0.001895 0.001226 0.002776 0.001445 0.002555
Mitotic Spindle 0.001199 0.001785 0.000456 0.008499 0.000218 0.003935 0.000283 0.001265 0.000807 0.002977 0.000431 0.004765
None 0.007675 0.008696 0.001071 0.007030 0.010406 0.001484 0.001207 0.003173 0.008766 0.004699 0.001091 0.005039
Nuclear Periphery 0.000649 0.000523 0.000669 0.002219 0.000290 0.001484 0.000323 0.000726 0.000505 0.001055 0.000619 0.001448
Nuclear Periphery Foci 0.000597 0.000451 0.000157 0.001175 0.000520 0.000384 0.000115 0.000076 0.000567 0.000414 0.000151 0.000607
Nucleolus 0.007178 0.008148 0.006403 0.003717 0.016349 0.008561 0.004100 0.003965 0.010841 0.008377 0.006067 0.003845
Nucleus 0.969799 0.956990 0.962013 0.922813 0.955462 0.949773 0.970717 0.952134 0.964073 0.952991 0.963284 0.937950
Peroxisomes 0.000274 0.000260 0.000276 0.000396 0.000427 0.000448 0.000320 0.000088 0.000335 0.000364 0.000283 0.000237
Vacuole 0.000271 0.000637 0.001256 0.003650 0.000650 0.003249 0.001853 0.001879 0.000422 0.002085 0.001343 0.002736
Vacuole Periphery 0.000138 0.000222 0.000123 0.001326 0.000265 0.001547 0.000106 0.000227 0.000188 0.000956 0.000120 0.000759

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.72 -15.59 -12.72 -7.54 1.98 1.57 -4.75 -3.37 -7.77 3.69 -3.39 -13.42 -12.09 -10.79 4.76
Bud Neck -7.11 -8.88 -15.86 -11.69 -2.99 -6.67 -4.89 -18.98 -13.68 -7.49 -9.75 -9.48 -24.49 -17.82 -4.58
Bud Site -3.36 -6.33 -5.16 -3.17 -3.20 -2.81 -3.32 -4.74 -1.34 -0.94 -4.27 -7.12 -6.96 -3.25 -3.55
Cell Periphery -3.37 3.19 0.13 3.62 -6.05 -0.42 1.40 1.29 2.95 -0.40 -3.06 3.66 1.18 4.53 -5.21
Cytoplasm 0.18 5.71 -0.78 -1.07 -8.04 2.55 1.97 1.58 -1.96 -0.98 2.38 6.39 0.78 -2.20 -8.70
Cytoplasmic Foci 0.77 2.29 1.01 0.28 -2.96 -0.94 1.86 2.69 3.08 1.20 0.09 2.96 2.04 2.47 -2.66
Eisosomes -5.83 -14.12 -12.01 -6.59 5.37 -1.74 -5.42 -5.14 -3.21 4.13 -5.82 -14.00 -13.05 -6.53 7.08
Endoplasmic Reticulum -11.50 -5.66 -13.87 -0.45 -0.26 -8.58 -2.67 -0.46 8.60 2.55 -14.02 -4.82 -10.47 7.28 0.89
Endosome -3.62 -0.65 -3.37 -2.48 -3.31 -3.22 -0.34 1.19 3.72 1.44 -4.30 0.40 -3.16 0.19 -3.29
Golgi -0.86 0.62 -1.47 -1.29 -1.54 -1.49 0.86 1.80 1.94 1.89 -1.73 1.39 -1.21 0.13 -1.47
Lipid Particles 1.09 2.44 -1.27 -1.41 -1.54 -1.73 0.39 3.77 3.22 2.29 -1.36 3.14 -0.85 -0.33 -1.40
Mitochondria 0.10 -1.24 -3.29 -3.62 -3.02 -2.56 0.06 -1.15 2.23 -3.09 -2.62 -0.84 -3.31 0.16 -3.96
Mitotic Spindle -0.65 1.04 -3.90 -3.25 -4.53 -3.65 -1.13 -3.61 0.83 -2.66 -2.96 0.52 -4.87 -1.87 -5.28
None -0.66 6.28 1.83 2.41 -5.61 5.10 5.19 4.35 -2.35 -2.70 3.74 8.15 4.69 0.99 -6.54
Nuclear Periphery 0.53 -0.10 -3.48 -4.26 -3.25 -5.97 -2.13 -9.40 2.67 -3.32 -3.09 -0.58 -4.45 -2.31 -3.00
Nuclear Periphery Foci 0.34 1.16 -0.46 -0.93 -1.70 0.83 1.16 1.55 2.65 0.88 0.77 1.67 0.41 -0.35 -1.52
Nucleolus -1.12 0.34 4.48 5.65 3.26 5.03 7.72 8.09 5.53 -0.15 3.17 4.63 9.12 7.85 3.18
Nucleus 3.57 2.75 9.97 7.03 6.94 1.01 -1.15 2.44 1.42 3.15 3.66 1.40 9.57 6.02 6.76
Peroxisomes 0.19 -0.20 0.05 -0.16 0.32 -0.09 0.11 3.21 4.93 3.97 -0.23 0.32 2.09 2.97 3.03
Vacuole -2.69 -6.80 -7.91 -6.91 -5.03 -3.35 -2.30 -3.22 0.96 0.99 -4.37 -4.74 -8.49 -3.05 -3.99
Vacuole Periphery -1.09 0.19 -2.24 -1.94 -2.29 -3.10 1.49 0.66 3.30 -1.36 -3.33 1.28 -2.04 1.68 -2.52
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp5

Prp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available