Standard name
Human Ortholog
Description Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0.07 0.09 0.12 0.17 0.17 0.13 0.1 0.08 0.08 0.1 0.17 0.28 0.34 – 0.27 0.22 0 0 0 0.09 0.05 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0.15 0 0 0 0 0 0 0 0 0 0 – 0.16 0.07 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.78 0.16 0.28 0.49 0.33 0.7 0.74 0.65 0.84 0.82 0.79 0.84 0.1 0.07 0.08 – 0.06 0.11 0 0 0 0 0.05 0.07
Nucleus 0.78 0.56 0.71 0.55 0.37 0.29 0.25 0.3 0.31 0.37 0.34 0.34 0.68 0.47 0.36 – 0.36 0.42 0.74 0.67 0.59 0.44 0.33 0.25
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.07 0
Vac/Vac Membrane 0 0.34 0.11 0.06 0.2 0.08 0.1 0.07 0.05 0.05 0.06 0.07 0.14 0.21 0.31 – 0.23 0.27 0.14 0.2 0.23 0.25 0.27 0.37
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 1 0
Bud 0 0 0 1 2 10 10 10 17 15 6 3 0 0 0 – 0 0 0 3 2 3 4 5
Bud Neck 0 0 0 0 0 0 0 3 0 1 0 0 0 0 0 – 0 0 0 1 1 0 2 3
Bud Site 0 0 0 0 1 6 7 9 11 24 3 13 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1 16 14 15 43 70 83 70 44 46 18 28 19 64 67 – 89 66 0 10 8 7 8 14
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 1 0 0 0 0 1 0 – 21 20 0 0 0 0 1 1
Endosome 0 9 3 3 55 0 3 21 0 3 0 0 0 5 7 – 53 22 1 5 3 0 9 4
Golgi 0 0 0 0 3 0 1 0 0 0 0 0 1 0 0 – 2 4 0 0 1 0 2 3
Mitochondria 14 40 59 87 118 295 365 359 388 459 169 245 11 16 15 – 20 32 1 2 7 2 9 10
Nucleus 14 143 147 97 133 123 123 163 142 206 72 98 76 106 71 – 119 123 41 202 153 36 59 40
Nuclear Periphery 0 2 0 0 0 1 0 4 2 4 2 3 0 0 0 – 0 0 0 1 0 0 0 1
Nucleolus 0 2 2 1 3 2 7 11 6 8 3 5 0 1 0 – 1 3 0 2 8 0 11 2
Peroxisomes 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 8 3 9 13 3 9 1 1 0 0 0 – 9 3 0 9 6 4 13 6
Vac/Vac Membrane 0 88 23 11 70 34 48 36 25 27 13 19 15 48 61 – 78 79 7 61 61 21 49 57
Unique Cell Count 18 257 208 176 355 422 494 549 461 562 213 290 111 226 198 332 295 57 304 261 84 182 157
Labelled Cell Count 29 300 248 216 437 545 657 701 639 802 288 415 122 241 221 393 352 57 304 261 84 182 157


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.3 4.5 4.1 4.2 3.6 3.2 3.7 3.3 3.1 3.4 3.1 4.5 4.4 4.3 4.8 5.6 5.8 4.2 4.3 5.7
Std Deviation (1e-4) 0.6 0.7 1.4 1.5 1.7 1.4 1.5 1.8 1.3 1.4 1.4 1.3 1.4 1.1 1.0 1.3 1.6 1.5 1.0 1.3 2.5
Intensity Change (Log2) – – – -0.11 -0.07 -0.31 -0.48 -0.27 -0.45 -0.54 -0.39 -0.52 0.02 -0.02 -0.04 0.09 0.34 0.38 -0.08 -0.04 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 3.6 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2821 -0.5321 -0.5551 -0.4681 -1.0014 -0.59 0.3872 1.4411 1.1393 1.1903 -0.0562 1.041 1.4705 1.3649 1.696 1.1846 0.9145 1.3408
Actin 0.0001 0.0002 0.0008 0.0003 0.0008 0.0004 0.039 0.0002 0.0145 0.0017 0.0082 0.0063 0.031 0.0002 0.035 0.0004 0.0002 0.0013
Bud 0.0001 0.0001 0.0001 0 0 0.0001 0.0004 0.0001 0.0002 0.0002 0.0002 0.0001 0.0008 0.0002 0.0004 0.0002 0.0001 0.0001
Bud Neck 0.0005 0.0004 0.0004 0.0005 0.0006 0.0037 0.0007 0.0002 0.0007 0.0007 0.0007 0.003 0.0023 0.0004 0.0009 0.0014 0.0007 0.0023
Bud Periphery 0 0.0001 0.0001 0 0 0.0001 0.0006 0 0.0002 0.0004 0.0003 0.0001 0.0011 0.0002 0.0004 0.0002 0.0001 0.0002
Bud Site 0.0003 0.0014 0.0005 0.0002 0.0001 0.0002 0.0021 0.0007 0.0014 0.0008 0.0005 0.0002 0.0084 0.001 0.0062 0.001 0.0004 0.0002
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0.0002 0 0.0001 0.0012 0.0001 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.0236 0.1325 0.0714 0.0772 0.0348 0.0353 0.0639 0.0923 0.0555 0.0568 0.0373 0.0619 0.0826 0.0889 0.0875 0.0488 0.042 0.0385
Cytoplasmic Foci 0.0003 0.0029 0.004 0.003 0.0056 0.0075 0.0191 0.0022 0.0283 0.0208 0.014 0.0042 0.0324 0.0019 0.015 0.008 0.0016 0.0011
Eisosomes 0 0 0.0004 0 0 0 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0022 0.0001 0.0003 0 0.0002 0
Endoplasmic Reticulum 0.0011 0.0078 0.0015 0.0029 0.001 0.0016 0.0033 0.0042 0.0048 0.0034 0.0012 0.0037 0.003 0.0056 0.0031 0.0016 0.0012 0.0013
Endosome 0.0006 0.0085 0.0053 0.0037 0.0011 0.0042 0.0241 0.0054 0.0253 0.0153 0.0122 0.007 0.0407 0.0043 0.0122 0.0451 0.0009 0.0023
Golgi 0 0.0001 0.0009 0.0001 0.0001 0.0001 0.0076 0.0001 0.0137 0.0053 0.0097 0.0032 0.0202 0.0001 0.0037 0.0023 0.0001 0.0002
Lipid Particles 0.0001 0.0013 0.0096 0.0005 0.0007 0.0009 0.0228 0.0004 0.0118 0.0034 0.0126 0.0017 0.0061 0.001 0.0123 0.0018 0.0004 0.0016
Mitochondria 0.0004 0.0005 0.0007 0.0008 0.0009 0.0006 0.0053 0.0006 0.0048 0.0136 0.0029 0.0012 0.0252 0.0009 0.0015 0.0009 0.0012 0.0014
None 0.0605 0.1515 0.2167 0.1126 0.0608 0.057 0.1144 0.4948 0.2028 0.3463 0.2459 0.159 0.1712 0.1564 0.1352 0.0502 0.3099 0.0726
Nuclear Periphery 0.0139 0.0338 0.0108 0.0325 0.0173 0.0228 0.0142 0.02 0.0354 0.0208 0.0101 0.0165 0.0088 0.027 0.0283 0.0077 0.0074 0.0172
Nucleolus 0.0069 0.0069 0.0067 0.0045 0.0059 0.0107 0.0074 0.0038 0.0063 0.0057 0.0057 0.0101 0.0176 0.009 0.0071 0.0046 0.0094 0.0103
Nucleus 0.8849 0.6302 0.6312 0.6868 0.8564 0.7912 0.6239 0.3408 0.5318 0.4506 0.5428 0.6629 0.4878 0.6852 0.5703 0.7577 0.6104 0.8242
Peroxisomes 0 0.0001 0.0011 0.0001 0.0004 0.0002 0.0193 0.0001 0.0093 0.0188 0.0166 0.0011 0.0027 0.0001 0.0083 0.0003 0.0001 0.0008
Punctate Nuclear 0.0052 0.0161 0.0359 0.0718 0.0118 0.0614 0.0242 0.0312 0.0498 0.0307 0.078 0.0559 0.0505 0.0128 0.0705 0.0629 0.0125 0.0229
Vacuole 0.0012 0.0048 0.0016 0.0019 0.0012 0.0014 0.0063 0.0021 0.0019 0.0032 0.0006 0.0015 0.0036 0.0035 0.0012 0.0042 0.001 0.001
Vacuole Periphery 0.0003 0.0007 0.0004 0.0004 0.0004 0.0005 0.0009 0.0007 0.001 0.0013 0.0004 0.0003 0.0008 0.0011 0.0005 0.0007 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7155 11.1665 8.286 7.6686 8.5477 14.5652 8.4678 9.102 8.5079 8.7071
Translational Efficiency 0.4003 0.2472 0.38 0.3481 0.4822 0.3477 0.4892 0.4774 0.4196 0.5506

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1863 550 1485 1618 1859 2285 425 1136 3722 2835 1910 2754

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 659.72 707.46 926.81 889.03 688.27 741.63 940.96 851.48 673.98 735.00 929.96 873.54
Standard Deviation 68.79 125.70 114.11 116.56 75.02 95.69 102.83 114.89 73.37 103.09 111.85 117.34
Intensity Change Log 2 — 0.100795 0.490420 0.430378 — 0.107725 0.451159 0.306998 — 0.104337 0.470507 0.368700

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000074 0.000841 0.000360 0.001520 0.000047 0.000204 0.000677 0.000773 0.000060 0.000328 0.000430 0.001212
Bud Neck 0.002291 0.008304 0.004768 0.016532 0.002916 0.010505 0.002215 0.012563 0.002603 0.010078 0.004200 0.014894
Bud Site 0.001505 0.004640 0.002164 0.021975 0.000497 0.005668 0.000929 0.011417 0.001002 0.005468 0.001889 0.017620
Cell Periphery 0.000139 0.000197 0.000073 0.000172 0.000087 0.000133 0.000068 0.000151 0.000113 0.000145 0.000072 0.000163
Cytoplasm 0.245746* 0.164342* 0.030887 0.086392 0.140493* 0.130878* 0.014580 0.093434 0.193176* 0.137370* 0.027258 0.089297
Cytoplasmic Foci 0.032196 0.049484 0.000496 0.005565 0.022407 0.021843 0.000269 0.006513 0.027307 0.027206 0.000445 0.005956
Eisosomes 0.000039 0.000080 0.000017 0.000034 0.000031 0.000031 0.000037 0.000037 0.000035 0.000041 0.000022 0.000035
Endoplasmic Reticulum 0.000432 0.005744 0.004314 0.007806 0.001139 0.001967 0.009032 0.005467 0.000785 0.002700 0.005363 0.006841
Endosome 0.001381 0.004140 0.000447 0.007555 0.000869 0.002484 0.000485 0.006358 0.001126 0.002805 0.000455 0.007061
Golgi 0.001308 0.001460 0.000038 0.003043 0.000265 0.000961 0.000047 0.003656 0.000787 0.001058 0.000040 0.003296
Lipid Particles 0.003383 0.008786 0.000276 0.000746 0.003825 0.003990 0.000526 0.002430 0.003604 0.004921 0.000331 0.001441
Mitochondria 0.003301 0.014831 0.000970 0.005953 0.002193 0.008850 0.000659 0.002801 0.002748 0.010011 0.000901 0.004653
Mitotic Spindle 0.002404 0.004602 0.002324 0.060239 0.001401 0.006608 0.001021 0.040593 0.001903 0.006219 0.002034 0.052135
None 0.012462 0.025415 0.003151 0.007214 0.007094 0.008048 0.004245 0.014459 0.009781 0.011417 0.003394 0.010202
Nuclear Periphery 0.001057 0.002607 0.001124 0.003580 0.001784 0.002520 0.001011 0.003251 0.001420 0.002537 0.001099 0.003444
Nuclear Periphery Foci 0.000415 0.001288 0.000247 0.002357 0.000487 0.000529 0.000348 0.003310 0.000451 0.000676 0.000270 0.002750
Nucleolus 0.003890 0.014708 0.001037 0.004047 0.003395 0.005651 0.001305 0.002871 0.003643 0.007408 0.001097 0.003562
Nucleus 0.682010* 0.657757* 0.939920* 0.725358* 0.806017* 0.775290* 0.950832* 0.760398* 0.743947* 0.752488* 0.942348* 0.739811*
Peroxisomes 0.002697 0.010792 0.000062 0.001815 0.001539 0.003402 0.000152 0.003485 0.002119 0.004836 0.000082 0.002504
Vacuole 0.003084 0.018519 0.007121 0.032987 0.002955 0.008987 0.011469 0.023197 0.003020 0.010836 0.008089 0.028949
Vacuole Periphery 0.000186 0.001461 0.000205 0.005109 0.000558 0.001450 0.000093 0.002839 0.000372 0.001452 0.000180 0.004173

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.90 -12.50 -10.75 -2.32 -7.33 -4.39 -7.97 -9.88 -7.47 -0.40 -5.49 -15.88 -14.04 -9.22 -7.33
Bud Neck -5.59 -7.95 -17.73 -9.79 -14.11 -6.95 -1.56 -9.48 -3.95 -9.08 -8.85 -7.10 -19.44 -9.46 -15.95
Bud Site -3.08 -1.29 -9.43 -6.62 -8.81 -5.35 -4.08 -7.00 -3.12 -6.26 -5.39 -2.59 -11.90 -6.60 -10.41
Cell Periphery -3.42 7.16 -1.08 2.84 -9.32 -3.36 1.10 -5.11 0.05 -6.08 -2.80 6.29 -4.26 0.21 -11.01
Cytoplasm 8.30 36.94 28.67 10.40 -9.53 1.79 27.35 10.51 9.22 -12.77 11.40 43.06 28.25 15.57 -14.43
Cytoplasmic Foci -3.71 18.63 16.20 10.84 -4.72 0.75 15.55 10.71 10.26 -4.42 0.41 24.13 19.39 15.66 -6.35
Eisosomes -7.10 7.56 -8.31 2.60 -14.86 0.26 -4.42 -7.93 -8.36 -2.74 -2.61 3.92 -11.74 -8.38 -15.03
Endoplasmic Reticulum -6.51 -22.57 -26.19 -5.53 -10.54 -2.87 -9.52 -14.66 -14.97 3.17 -8.31 -19.32 -28.44 -20.42 -5.95
Endosome -4.75 3.97 -5.39 0.02 -7.42 -5.46 1.80 -4.78 -2.11 -5.23 -6.31 4.56 -7.36 -2.83 -9.08
Golgi -0.27 3.34 0.48 0.78 -3.37 -2.76 1.84 -2.27 -0.95 -2.69 -0.99 3.75 -1.36 -0.57 -4.22
Lipid Particles -4.77 7.80 5.48 7.16 -10.84 0.00 6.44 3.93 4.23 -2.73 -2.28 10.08 6.54 7.82 -6.37
Mitochondria -3.62 3.29 -0.86 3.44 -6.32 -6.39 3.50 -0.24 6.56 -5.89 -6.88 4.33 -1.15 6.58 -7.57
Mitotic Spindle -1.43 -0.27 -13.25 -12.38 -13.07 -4.16 -0.50 -8.90 -7.55 -8.53 -4.22 -0.75 -15.97 -14.09 -15.52
None -3.09 11.54 5.52 4.54 -8.59 -0.77 2.92 -2.43 -1.85 -4.63 -1.18 11.07 1.38 2.16 -8.99
Nuclear Periphery -5.21 -3.26 -11.98 -7.31 -11.32 -3.84 0.58 -4.93 -2.94 -5.36 -6.96 -1.90 -12.54 -8.38 -12.35
Nuclear Periphery Foci -4.00 0.25 -7.64 -5.77 -7.74 0.22 0.54 -4.57 -4.66 -4.73 -2.08 0.81 -8.87 -8.41 -9.08
Nucleolus -4.72 6.03 -7.26 0.01 -9.81 -2.72 5.63 -4.60 -3.21 -6.51 -5.02 8.54 -8.63 -4.71 -11.82
Nucleus 1.83 -33.76 -2.29 -3.27 26.46 3.88 -16.50 4.80 1.85 16.81 -1.10 -36.11 2.36 3.12 31.27
Peroxisomes -4.35 4.66 2.84 5.50 -3.80 -3.75 5.53 0.06 2.20 -1.92 -4.75 6.66 2.06 6.25 -3.35
Vacuole -6.45 -7.42 -17.57 -7.73 -12.71 -7.46 -6.60 -12.22 -9.70 -3.56 -9.88 -9.78 -21.33 -15.92 -13.13
Vacuole Periphery -3.83 0.12 -2.78 -0.41 -2.77 -2.41 1.67 -1.41 0.42 -2.74 -4.19 1.43 -2.95 -0.09 -3.69
Standard name
Human Ortholog
Description Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p
Localization
Cell Percentages nucleus (78%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ert1

Ert1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ert1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available