Standard name | |
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Human Ortholog | |
Description | ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 2 | 5 | 2 | 5 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 5 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 5 | 11 | 8 | 19 | 13 | 16 | 10 | 15 | 0 | 3 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 14 | 5 | 10 | 14 | 3 | 10 | 12 | 10 | 7 | 11 | 2 | 31 | 59 | 55 | 29 | 8 | 22 | 2 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 5 | 2 | 1 | 0 | 3 | 2 | 1 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 0 | 14 | 20 | 11 | 8 | 46 | 55 | 92 | 98 | 104 | 59 | 64 | 2 | 0 | 4 | 6 | 0 | 7 | 0 | 3 | 1 | 1 | 3 | 5 |
Nucleus | 50 | 362 | 425 | 196 | 269 | 317 | 320 | 401 | 211 | 233 | 195 | 244 | 312 | 368 | 368 | 224 | 127 | 240 | 47 | 388 | 428 | 177 | 144 | 209 |
Nuclear Periphery | 0 | 0 | 4 | 0 | 2 | 3 | 5 | 4 | 9 | 8 | 0 | 4 | 1 | 2 | 2 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 23 | 104 | 99 | 71 | 71 | 147 | 168 | 269 | 187 | 208 | 157 | 236 | 84 | 114 | 134 | 56 | 20 | 67 | 13 | 33 | 54 | 60 | 111 | 153 |
Peroxisomes | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 3 | 2 | 3 | 0 | 2 | 4 | 9 | 11 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 4 |
Vac/Vac Membrane | 1 | 10 | 4 | 2 | 5 | 2 | 3 | 2 | 0 | 0 | 1 | 0 | 1 | 6 | 3 | 7 | 7 | 10 | 1 | 12 | 10 | 12 | 18 | 37 |
Unique Cell Count | 64 | 423 | 482 | 238 | 324 | 421 | 439 | 615 | 375 | 409 | 314 | 396 | 370 | 475 | 493 | 296 | 158 | 315 | 68 | 448 | 505 | 258 | 287 | 426 |
Labelled Cell Count | 78 | 509 | 562 | 291 | 380 | 531 | 572 | 811 | 533 | 584 | 437 | 575 | 438 | 568 | 596 | 339 | 167 | 353 | 68 | 448 | 505 | 258 | 287 | 426 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.9 | 10.8 | 7.9 | 7.5 | 8.0 | 6.9 | 6.8 | 6.3 | 6.3 | 6.1 | 6.1 | 5.7 | 11.7 | 11.3 | 9.8 | 15.5 | 18.1 | 15.6 | 11.4 | 12.4 | 12.9 |
Std Deviation (1e-4) | 3.1 | 3.9 | 2.1 | 2.0 | 2.2 | 1.9 | 2.1 | 1.6 | 1.8 | 2.0 | 2.2 | 1.7 | 3.8 | 4.2 | 3.5 | 5.7 | 5.9 | 4.8 | 2.9 | 2.8 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.01 | -0.21 | -0.23 | -0.33 | -0.33 | -0.38 | -0.39 | -0.49 | 0.57 | 0.51 | 0.31 | 0.96 | 1.19 | 0.98 | 0.52 | 0.65 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 3.6 | 0 | 3.9 | 4.1 | 4.4 | 0 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 3.0 | 0 | 1.5 | 1.2 | 1.8 | 0.9 | 2.4 | 0 | 5.3 | 7.0 | 6.6 | 5.8 | 0 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.3 | -1.3 | 3.9 | 4.6 | 5.9 | 9.3 | 9.1 | 6.8 | 6.0 | -3.3 | -4.5 | -3.4 | -1.6 | 0 | -1.5 |
Nucleus | -2.1 | -2.1 | -5.0 | -5.9 | -8.8 | -10.6 | -10.6 | -8.7 | -9.2 | -1.6 | -4.4 | -5.4 | -4.6 | -2.5 | -4.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1.9 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.8 | 0.5 | 4.8 | 5.9 | 8.1 | 9.0 | 9.5 | 8.7 | 11.9 | 0.8 | 1.3 | 2.4 | -0.5 | -2.2 | 0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.6123 | 9.4563 | 8.8985 | 7.9385 | 8.3627 | 9.2581 | 12.4653 | 11.936 | 12.1342 | 11.0683 | 10.5306 | 11.2351 | 14.084 | 12.0718 | 12.2997 | 10.7105 | 9.5618 | 11.5154 |
Actin | 0.0265 | 0 | 0.0264 | 0.0373 | 0.068 | 0.0005 | 0.0247 | 0.0049 | 0.012 | 0.0035 | 0.0098 | 0.0038 | 0 | 0 | 0 | 0.0001 | 0.001 | 0.0002 |
Bud | 0.001 | 0 | 0.0005 | 0.0007 | 0.0017 | 0.0001 | 0.0008 | 0 | 0.0005 | 0.0006 | 0.0005 | 0.0001 | 0 | 0 | 0 | 0.0032 | 0.0001 | 0 |
Bud Neck | 0.0066 | 0 | 0.0004 | 0.0017 | 0.0012 | 0.0004 | 0.0019 | 0.0001 | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0.0007 | 0.0004 | 0.0003 |
Bud Periphery | 0.0019 | 0 | 0.0009 | 0.0017 | 0.0023 | 0.0001 | 0.0017 | 0.0001 | 0.001 | 0.001 | 0.0007 | 0.0004 | 0 | 0 | 0 | 0.0083 | 0.0002 | 0.0001 |
Bud Site | 0.0089 | 0 | 0.0037 | 0.0378 | 0.0136 | 0.0001 | 0.0069 | 0.0001 | 0.0031 | 0.0011 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0.0002 | 0.0001 |
Cell Periphery | 0.0013 | 0 | 0.0008 | 0.0015 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0028 | 0.0001 | 0 |
Cytoplasm | 0.0021 | 0 | 0.0004 | 0.0011 | 0.0032 | 0 | 0.0052 | 0.0022 | 0.0047 | 0.0002 | 0.0074 | 0.0009 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 |
Cytoplasmic Foci | 0.002 | 0 | 0.0044 | 0.0087 | 0.0182 | 0.0002 | 0.0202 | 0.0002 | 0.0052 | 0.0018 | 0.0142 | 0.0011 | 0 | 0 | 0 | 0.0001 | 0.0039 | 0.0002 |
Eisosomes | 0.0008 | 0 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0107 | 0 | 0.0006 | 0.0011 | 0.0024 | 0.0001 | 0.0031 | 0.0001 | 0.0018 | 0.0002 | 0.0042 | 0.0007 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Endosome | 0.0029 | 0 | 0.0012 | 0.0151 | 0.0091 | 0.0009 | 0.0196 | 0.0002 | 0.0032 | 0.0085 | 0.0281 | 0.0038 | 0 | 0 | 0 | 0.0001 | 0.005 | 0.0001 |
Golgi | 0.0036 | 0 | 0.0018 | 0.0058 | 0.0039 | 0.0004 | 0.0061 | 0.0005 | 0.0018 | 0.0057 | 0.0117 | 0.0014 | 0 | 0 | 0 | 0 | 0.0072 | 0.0001 |
Lipid Particles | 0.0053 | 0 | 0.007 | 0.0117 | 0.0121 | 0.0016 | 0.0127 | 0.0001 | 0.0052 | 0.0015 | 0.0121 | 0.0019 | 0 | 0 | 0 | 0 | 0.0569 | 0.0064 |
Mitochondria | 0.0083 | 0.0001 | 0.0158 | 0.0191 | 0.0025 | 0.0003 | 0.0128 | 0.0005 | 0.004 | 0.0086 | 0.0026 | 0.0078 | 0.0002 | 0.0001 | 0.0001 | 0.0056 | 0.0284 | 0.0088 |
None | 0.0015 | 0 | 0.0006 | 0.0021 | 0.0072 | 0.0001 | 0.003 | 0.0027 | 0.0022 | 0.0001 | 0.0058 | 0.0005 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0117 | 0.0001 | 0.0016 | 0.0106 | 0.006 | 0.0023 | 0.0107 | 0.0005 | 0.0035 | 0.0003 | 0.0286 | 0.0041 | 0 | 0.0004 | 0.0001 | 0.0007 | 0.0008 | 0.0004 |
Nucleolus | 0.1156 | 0.0445 | 0.0419 | 0.0565 | 0.3309 | 0.1975 | 0.0387 | 0.0258 | 0.0243 | 0.0216 | 0.222 | 0.0976 | 0.0212 | 0.0542 | 0.0155 | 0.0832 | 0.1421 | 0.1269 |
Nucleus | 0.7793 | 0.955 | 0.8807 | 0.7544 | 0.4925 | 0.7933 | 0.7975 | 0.959 | 0.9156 | 0.9428 | 0.6157 | 0.8671 | 0.9786 | 0.9451 | 0.9843 | 0.8824 | 0.7436 | 0.8549 |
Peroxisomes | 0.0005 | 0 | 0.0069 | 0.0064 | 0.0027 | 0.0002 | 0.0059 | 0.0001 | 0.0012 | 0.0009 | 0.0063 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0.0076 | 0.0003 |
Punctate Nuclear | 0.0029 | 0.0001 | 0.0009 | 0.0116 | 0.019 | 0.0015 | 0.0214 | 0.0017 | 0.0073 | 0.0002 | 0.0235 | 0.0066 | 0 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0004 |
Vacuole | 0.0029 | 0 | 0.0016 | 0.0035 | 0.0016 | 0.0002 | 0.0039 | 0.0004 | 0.0014 | 0.0007 | 0.0029 | 0.0004 | 0 | 0 | 0 | 0.0069 | 0.0007 | 0.0001 |
Vacuole Periphery | 0.004 | 0 | 0.0016 | 0.0111 | 0.001 | 0.0001 | 0.0024 | 0.0005 | 0.001 | 0.0005 | 0.0025 | 0.0005 | 0 | 0 | 0 | 0.0035 | 0.0009 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 77.6289 | 37.2437 | 39.5115 | 39.6963 | 54.2282 | 61.1579 | 34.8622 | 33.6563 | 34.7739 | 50.3044 |
Translational Efficiency | 1.2697 | 1.4586 | 0.9832 | 1.1747 | 1.1154 | 1.3127 | 1.1316 | 0.851 | 1.0005 | 1.0633 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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