Standard name
Human Ortholog
Description ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.11 0.1 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.11 0.13 0.15 0.26 0.25 0.19 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.78 0.86 0.88 0.82 0.83 0.75 0.73 0.65 0.56 0.57 0.62 0.62 0.84 0.77 0.75 0.76 0.8 0.76 0.7 0.87 0.85 0.69 0.51 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.36 0.25 0.21 0.3 0.22 0.35 0.38 0.44 0.5 0.51 0.5 0.6 0.23 0.24 0.27 0.19 0.13 0.21 0.2 0.08 0.11 0.24 0.39 0.36
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 1 1 3 2 5 2 5 0 1 5 0 0 0 0 3 2 1 0 2
Bud Neck 0 0 0 0 2 1 2 5 1 0 1 3 0 0 0 2 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 5 11 8 19 13 16 10 15 0 3 4 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 4 14 5 10 14 3 10 12 10 7 11 2 31 59 55 29 8 22 2 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 2 2 2 0 0 1 0 1 1
Endosome 0 1 4 0 0 0 0 0 0 0 0 0 2 3 3 5 2 1 0 3 2 1 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 3
Mitochondria 0 14 20 11 8 46 55 92 98 104 59 64 2 0 4 6 0 7 0 3 1 1 3 5
Nucleus 50 362 425 196 269 317 320 401 211 233 195 244 312 368 368 224 127 240 47 388 428 177 144 209
Nuclear Periphery 0 0 4 0 2 3 5 4 9 8 0 4 1 2 2 4 1 1 0 0 0 0 0 0
Nucleolus 23 104 99 71 71 147 168 269 187 208 157 236 84 114 134 56 20 67 13 33 54 60 111 153
Peroxisomes 0 2 0 0 0 0 0 0 0 0 0 0 1 3 6 2 0 0 0 0 0 0 0 0
SpindlePole 0 2 1 0 2 0 0 3 2 3 0 2 4 9 11 0 0 0 1 0 2 1 2 4
Vac/Vac Membrane 1 10 4 2 5 2 3 2 0 0 1 0 1 6 3 7 7 10 1 12 10 12 18 37
Unique Cell Count 64 423 482 238 324 421 439 615 375 409 314 396 370 475 493 296 158 315 68 448 505 258 287 426
Labelled Cell Count 78 509 562 291 380 531 572 811 533 584 437 575 438 568 596 339 167 353 68 448 505 258 287 426


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.9 10.8 7.9 7.5 8.0 6.9 6.8 6.3 6.3 6.1 6.1 5.7 11.7 11.3 9.8 15.5 18.1 15.6 11.4 12.4 12.9
Std Deviation (1e-4) 3.1 3.9 2.1 2.0 2.2 1.9 2.1 1.6 1.8 2.0 2.2 1.7 3.8 4.2 3.5 5.7 5.9 4.8 2.9 2.8 3.1
Intensity Change (Log2) -0.09 0.01 -0.21 -0.23 -0.33 -0.33 -0.38 -0.39 -0.49 0.57 0.51 0.31 0.96 1.19 0.98 0.52 0.65 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.6 0 3.9 4.1 4.4 0 4.3 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3.0 0 1.5 1.2 1.8 0.9 2.4 0 5.3 7.0 6.6 5.8 0 4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 -1.3 3.9 4.6 5.9 9.3 9.1 6.8 6.0 -3.3 -4.5 -3.4 -1.6 0 -1.5
Nucleus -2.1 -2.1 -5.0 -5.9 -8.8 -10.6 -10.6 -8.7 -9.2 -1.6 -4.4 -5.4 -4.6 -2.5 -4.4
Nuclear Periphery 0 0 0 0 0 1.9 1.5 0 0 0 0 0 0 0 0
Nucleolus 2.8 0.5 4.8 5.9 8.1 9.0 9.5 8.7 11.9 0.8 1.3 2.4 -0.5 -2.2 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6123 9.4563 8.8985 7.9385 8.3627 9.2581 12.4653 11.936 12.1342 11.0683 10.5306 11.2351 14.084 12.0718 12.2997 10.7105 9.5618 11.5154
Actin 0.0265 0 0.0264 0.0373 0.068 0.0005 0.0247 0.0049 0.012 0.0035 0.0098 0.0038 0 0 0 0.0001 0.001 0.0002
Bud 0.001 0 0.0005 0.0007 0.0017 0.0001 0.0008 0 0.0005 0.0006 0.0005 0.0001 0 0 0 0.0032 0.0001 0
Bud Neck 0.0066 0 0.0004 0.0017 0.0012 0.0004 0.0019 0.0001 0.0005 0.0002 0.0006 0.0003 0 0 0 0.0007 0.0004 0.0003
Bud Periphery 0.0019 0 0.0009 0.0017 0.0023 0.0001 0.0017 0.0001 0.001 0.001 0.0007 0.0004 0 0 0 0.0083 0.0002 0.0001
Bud Site 0.0089 0 0.0037 0.0378 0.0136 0.0001 0.0069 0.0001 0.0031 0.0011 0.0007 0.0002 0 0 0 0.0012 0.0002 0.0001
Cell Periphery 0.0013 0 0.0008 0.0015 0.0004 0.0001 0.0005 0.0001 0.0004 0.0001 0.0001 0.0001 0 0 0 0.0028 0.0001 0
Cytoplasm 0.0021 0 0.0004 0.0011 0.0032 0 0.0052 0.0022 0.0047 0.0002 0.0074 0.0009 0 0 0 0.0004 0.0001 0
Cytoplasmic Foci 0.002 0 0.0044 0.0087 0.0182 0.0002 0.0202 0.0002 0.0052 0.0018 0.0142 0.0011 0 0 0 0.0001 0.0039 0.0002
Eisosomes 0.0008 0 0.0004 0.0004 0.0003 0 0.0003 0 0.0002 0 0 0.0001 0 0 0 0 0 0
Endoplasmic Reticulum 0.0107 0 0.0006 0.0011 0.0024 0.0001 0.0031 0.0001 0.0018 0.0002 0.0042 0.0007 0 0 0 0.0002 0.0001 0
Endosome 0.0029 0 0.0012 0.0151 0.0091 0.0009 0.0196 0.0002 0.0032 0.0085 0.0281 0.0038 0 0 0 0.0001 0.005 0.0001
Golgi 0.0036 0 0.0018 0.0058 0.0039 0.0004 0.0061 0.0005 0.0018 0.0057 0.0117 0.0014 0 0 0 0 0.0072 0.0001
Lipid Particles 0.0053 0 0.007 0.0117 0.0121 0.0016 0.0127 0.0001 0.0052 0.0015 0.0121 0.0019 0 0 0 0 0.0569 0.0064
Mitochondria 0.0083 0.0001 0.0158 0.0191 0.0025 0.0003 0.0128 0.0005 0.004 0.0086 0.0026 0.0078 0.0002 0.0001 0.0001 0.0056 0.0284 0.0088
None 0.0015 0 0.0006 0.0021 0.0072 0.0001 0.003 0.0027 0.0022 0.0001 0.0058 0.0005 0 0 0 0.0002 0.0001 0.0001
Nuclear Periphery 0.0117 0.0001 0.0016 0.0106 0.006 0.0023 0.0107 0.0005 0.0035 0.0003 0.0286 0.0041 0 0.0004 0.0001 0.0007 0.0008 0.0004
Nucleolus 0.1156 0.0445 0.0419 0.0565 0.3309 0.1975 0.0387 0.0258 0.0243 0.0216 0.222 0.0976 0.0212 0.0542 0.0155 0.0832 0.1421 0.1269
Nucleus 0.7793 0.955 0.8807 0.7544 0.4925 0.7933 0.7975 0.959 0.9156 0.9428 0.6157 0.8671 0.9786 0.9451 0.9843 0.8824 0.7436 0.8549
Peroxisomes 0.0005 0 0.0069 0.0064 0.0027 0.0002 0.0059 0.0001 0.0012 0.0009 0.0063 0.0006 0 0 0 0.0002 0.0076 0.0003
Punctate Nuclear 0.0029 0.0001 0.0009 0.0116 0.019 0.0015 0.0214 0.0017 0.0073 0.0002 0.0235 0.0066 0 0.0001 0 0.0002 0.0007 0.0004
Vacuole 0.0029 0 0.0016 0.0035 0.0016 0.0002 0.0039 0.0004 0.0014 0.0007 0.0029 0.0004 0 0 0 0.0069 0.0007 0.0001
Vacuole Periphery 0.004 0 0.0016 0.0111 0.001 0.0001 0.0024 0.0005 0.001 0.0005 0.0025 0.0005 0 0 0 0.0035 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.6289 37.2437 39.5115 39.6963 54.2282 61.1579 34.8622 33.6563 34.7739 50.3044
Translational Efficiency 1.2697 1.4586 0.9832 1.1747 1.1154 1.3127 1.1316 0.851 1.0005 1.0633

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; Isw1p import into nucleus depends on C-terminal bipartite nuclear targeting signal KRIR X19 KKAK
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Isw1

Isw1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isw1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available