Standard name
Human Ortholog
Description Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.17 0.07 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.13 0.17 0.12 0.29 0.1 0.12 0.21 0.23 0.19
Bud 0.06 0.05 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.05 0.08
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.12 0.05 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.09 0.08 0.2 0.15 0.12 0.17
Cytoplasm 0.45 0.67 0.9 0.92 0.93 0.9 0.91 0.9 0.75 0.71 0.67 0.68 0.82 0.81 0.86 0.14 0.16 0.15 0.06 0.22 0.2 0.13 0.1 0.14
Endoplasmic Reticulum 0.27 0.1 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.08 0.05 0.05 0.27 0.15 0.1 0.14 0.15 0.13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.35 0.25 0.32 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0 0 0 0 0
Mitochondria 0.15 0.11 0 0 0 0.09 0.1 0.05 0.29 0.3 0.37 0.34 0 0 0 0.09 0.15 0.1 0.11 0.2 0.21 0.19 0.23 0.17
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.29 0.28 0.32 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0.06 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 10 10 11 11 5 9 7 5 3 2 1 15 5 7 32 22 13 37 21 50 54 87 81
Bud 5 7 4 3 3 3 9 8 27 26 23 18 10 8 6 8 1 1 0 0 2 3 3 3
Bud Neck 0 4 16 14 30 16 14 15 13 8 3 4 0 2 1 2 1 1 5 6 24 19 18 31
Bud Site 1 0 0 0 0 2 0 0 2 1 1 2 0 0 0 0 0 1 – – – – – –
Cell Periphery 3 7 42 14 25 20 18 11 8 5 3 6 13 9 15 0 1 0 10 17 82 38 46 69
Cytoplasm 35 99 319 287 437 440 571 527 427 350 365 329 182 323 366 36 21 17 7 45 86 32 38 59
Endoplasmic Reticulum 21 14 11 2 5 5 6 8 0 3 0 1 14 5 22 21 6 6 34 30 42 36 56 55
Endosome 0 2 0 0 1 0 0 0 0 0 1 1 4 34 20 88 33 35 5 10 7 3 10 14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 5 10 9 1 3 1 2 2 4
Mitochondria 12 16 0 3 6 42 61 31 166 148 199 166 9 3 3 23 20 11 14 41 89 50 89 69
Nucleus 0 1 0 0 0 1 1 7 2 3 7 4 0 0 0 2 1 3 0 2 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 1 0 0 2 0 0 0 0 0 0 3 1 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 29 7 25 2 1 2 1 0 2 6 4
SpindlePole 2 3 4 7 3 3 6 7 6 9 9 14 4 25 12 74 37 35 0 2 1 0 2 2
Vac/Vac Membrane 0 0 0 0 0 1 0 2 5 6 7 7 0 1 0 10 7 2 5 18 23 13 13 16
Unique Cell Count 78 147 356 311 469 489 627 587 566 493 544 487 223 398 427 255 131 111 128 205 423 261 381 419
Labelled Cell Count 93 164 406 341 522 538 696 623 663 564 620 553 251 446 459 326 166 136 128 205 423 261 381 419


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.6 5.1 4.5 5.0 4.0 4.2 4.6 3.6 3.6 3.4 4.0 4.4 4.8 4.7 7.2 7.6 7.2 4.5 4.3 4.7
Std Deviation (1e-4) 0.6 1.4 0.7 0.8 0.9 0.9 0.9 0.9 0.7 0.8 0.5 0.8 0.6 1.7 1.0 1.4 1.8 1.3 2.2 1.2 1.1
Intensity Change (Log2) – – – -0.18 -0.03 -0.34 -0.3 -0.17 -0.52 -0.49 -0.58 -0.35 -0.22 -0.1 -0.11 0.49 0.58 0.5 -0.18 -0.26 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.358 -0.2881 -0.372 -0.039 -0.2532 -0.3724 -0.2059 -0.3089 -0.3829 -0.6213 -0.993 -0.2764 -0.5199 0.0048 -0.2545 0.0208 -0.6373 -0.3835
Actin 0.0189 0.0166 0.018 0.0287 0.0086 0.0116 0.0663 0.0266 0.0453 0.0438 0.0028 0.0197 0.02 0.0147 0.0355 0.0063 0.0178 0.0154
Bud 0.0007 0.0009 0.0007 0.0015 0.0007 0.0002 0.001 0.0078 0.0017 0.0004 0.0002 0.0021 0.0002 0.0003 0.0007 0.0002 0.0001 0.0002
Bud Neck 0.008 0.003 0.0089 0.0013 0.0036 0.0087 0.0056 0.0035 0.0095 0.0079 0.0028 0.0196 0.0036 0.005 0.004 0.0062 0.001 0.0058
Bud Periphery 0.0015 0.0015 0.0015 0.015 0.0013 0.0004 0.0019 0.004 0.0029 0.0007 0.0005 0.0055 0.0002 0.0007 0.0009 0.0003 0.0001 0.0005
Bud Site 0.0082 0.0287 0.0152 0.0011 0.0182 0.0003 0.0173 0.0281 0.0113 0.0007 0.0016 0.0011 0.0012 0.0104 0.0047 0.0014 0.0003 0.0006
Cell Periphery 0.0021 0.0025 0.0029 0.0006 0.0008 0.0009 0.0021 0.0023 0.0019 0.0028 0.0012 0.0021 0.0009 0.0156 0.0019 0.0005 0.0002 0.0031
Cytoplasm 0.1059 0.1142 0.0984 0.0367 0.081 0.2009 0.1659 0.1446 0.1764 0.1038 0.2865 0.2108 0.0943 0.2449 0.1764 0.1738 0.1098 0.2648
Cytoplasmic Foci 0.2188 0.2138 0.2092 0.2131 0.2446 0.2033 0.1864 0.2519 0.2351 0.2972 0.1868 0.2673 0.2351 0.1787 0.2109 0.3591 0.2828 0.2613
Eisosomes 0.0026 0.0012 0.0016 0.0003 0.0006 0.0004 0.0007 0.0004 0.0004 0.0013 0.0002 0.0004 0.0004 0.0007 0.0006 0.0001 0.0001 0.0003
Endoplasmic Reticulum 0.0096 0.0136 0.0144 0.0041 0.012 0.0125 0.0116 0.0101 0.0126 0.0045 0.0039 0.0118 0.013 0.0397 0.0227 0.0616 0.0043 0.0148
Endosome 0.1063 0.1639 0.1384 0.169 0.1259 0.088 0.1451 0.157 0.1654 0.1984 0.1293 0.1121 0.1488 0.1106 0.1397 0.1617 0.1087 0.105
Golgi 0.0178 0.02 0.0219 0.064 0.0166 0.0157 0.0318 0.0327 0.0334 0.0239 0.0124 0.0305 0.0285 0.018 0.0262 0.0135 0.0227 0.017
Lipid Particles 0.1808 0.128 0.1283 0.1148 0.1879 0.1166 0.152 0.1132 0.1028 0.1121 0.1558 0.096 0.2369 0.0931 0.1239 0.0789 0.1508 0.0989
Mitochondria 0.0147 0.0116 0.0073 0.046 0.0115 0.0067 0.0057 0.0046 0.0047 0.0024 0.0007 0.0104 0.0031 0.0014 0.0037 0.0011 0.0011 0.0025
None 0.2295 0.2415 0.2807 0.2376 0.1897 0.2903 0.1172 0.1649 0.1335 0.122 0.1563 0.1654 0.1531 0.24 0.2093 0.0609 0.244 0.1677
Nuclear Periphery 0.0096 0.0021 0.0032 0.0028 0.0096 0.0011 0.0267 0.001 0.0063 0.0013 0.0009 0.0013 0.0055 0.0016 0.0018 0.0042 0.0006 0.0019
Nucleolus 0.0011 0.001 0.0014 0.0006 0.0107 0.0005 0.0009 0.0011 0.0006 0.0002 0.0005 0.0003 0.0006 0.0011 0.0002 0.0001 0.0001 0.0004
Nucleus 0.006 0.0011 0.0022 0.0009 0.0039 0.0011 0.0044 0.0017 0.0032 0.0005 0.0011 0.001 0.0031 0.0009 0.0007 0.0005 0.0004 0.001
Peroxisomes 0.0377 0.0208 0.0293 0.0468 0.0369 0.0296 0.0401 0.031 0.0398 0.0668 0.0375 0.0301 0.026 0.0094 0.0269 0.0593 0.0496 0.0233
Punctate Nuclear 0.0126 0.0038 0.0057 0.0024 0.0278 0.0047 0.0068 0.0041 0.0033 0.0027 0.0161 0.0029 0.0203 0.0032 0.0029 0.0051 0.0036 0.0095
Vacuole 0.006 0.0093 0.0092 0.0079 0.0062 0.0058 0.0079 0.0087 0.0085 0.006 0.0028 0.0085 0.0044 0.0095 0.0057 0.0044 0.0017 0.0053
Vacuole Periphery 0.0014 0.0009 0.0016 0.0046 0.002 0.0006 0.0026 0.0007 0.0014 0.0008 0.0003 0.001 0.0008 0.0006 0.0007 0.0008 0.0003 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.9691 8.0424 5.9678 8.2015 2.7446 4.2313 7.4142 8.3939 9.0159 8.8908
Translational Efficiency 0.8402 0.4743 0.6184 0.4387 1.1291 0.8221 0.7595 0.4469 0.5583 0.403

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1426 1192 270 912 1340 382 866 951 2766 1574 1136 1863

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 656.15 741.44 1055.09 863.83 701.30 785.87 881.41 836.19 678.02 752.22 922.69 849.72
Standard Deviation 66.26 109.80 111.00 128.16 90.01 100.35 105.40 115.48 81.84 109.25 129.85 122.63
Intensity Change Log 2 — 0.176304 0.685269 0.396722 — 0.164259 0.329782 0.253799 — 0.170094 0.512552 0.324653

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.009068 0.027672 0.002780 0.049385 0.009784 0.009557 0.004372 0.012803 0.009415 0.023275 0.003994 0.030711
Bud Neck 0.034866 0.013476 0.012082 0.016760 0.023302 0.032764 0.007603 0.018542 0.029264 0.018157 0.008667 0.017670
Bud Site 0.008116 0.009794 0.023499 0.069285 0.010013 0.010729 0.015027 0.045977 0.009035 0.010021 0.017040 0.057387
Cell Periphery 0.003006 0.000509 0.002082 0.001328 0.001528 0.001330 0.000697 0.000606 0.002290 0.000708 0.001026 0.000959
Cytoplasm 0.321273* 0.109668* 0.177563* 0.174151* 0.291639* 0.180387* 0.397995* 0.194103* 0.306917* 0.126831* 0.345603* 0.184336*
Cytoplasmic Foci 0.249335* 0.346978* 0.043937 0.195011* 0.298449* 0.311551* 0.124073 0.222550* 0.273128* 0.338380* 0.105027 0.209068*
Eisosomes 0.023061 0.004641 0.000413 0.001728 0.007553 0.009081 0.000420 0.000651 0.015548 0.005719 0.000418 0.001178
Endoplasmic Reticulum 0.007944 0.006115 0.037820 0.018114 0.008304 0.004513 0.008088 0.005532 0.008118 0.005726 0.015154 0.011691
Endosome 0.021883 0.073043 0.014037 0.038861 0.031042 0.066581 0.013519 0.067974 0.026320 0.071475 0.013642 0.053722
Golgi 0.070836 0.090219 0.000317 0.010120 0.064859 0.090548 0.001007 0.009594 0.067940 0.090299 0.000843 0.009851
Lipid Particles 0.117880* 0.137228* 0.013187 0.059917 0.113694* 0.111783* 0.017831 0.061563 0.115852* 0.131052* 0.016727 0.060757
Mitochondria 0.016213 0.011994 0.000608 0.002109 0.008886 0.020595 0.000498 0.002529 0.012664 0.014081 0.000524 0.002323
Mitotic Spindle 0.000378 0.005830 0.004240 0.027851 0.000494 0.000564 0.005337 0.051556 0.000434 0.004552 0.005076 0.039952
None 0.002334 0.002128 0.000572 0.000413 0.002840 0.000699 0.002801 0.001639 0.002579 0.001782 0.002271 0.001039
Nuclear Periphery 0.000253 0.000198 0.002384 0.000614 0.000403 0.000286 0.001599 0.001276 0.000325 0.000219 0.001785 0.000952
Nuclear Periphery Foci 0.000624 0.001217 0.017081 0.012919 0.001375 0.000799 0.011153 0.010580 0.000988 0.001115 0.012562 0.011725
Nucleolus 0.000561 0.000390 0.000366 0.000556 0.000772 0.000295 0.000867 0.003164 0.000663 0.000367 0.000748 0.001888
Nucleus 0.031283 0.014173 0.278266* 0.054074 0.044854 0.016978 0.210752* 0.066824 0.037857 0.014854 0.226798* 0.060582
Peroxisomes 0.062016 0.121147* 0.001307 0.059298 0.056386 0.086743 0.012147 0.065296 0.059289 0.112798* 0.009570 0.062360
Vacuole 0.017958 0.022238 0.367190* 0.204521* 0.022771 0.037228 0.164045 0.154050 0.020290 0.025876 0.212328* 0.178757
Vacuole Periphery 0.001112 0.001342 0.000271 0.002983 0.001053 0.006989 0.000171 0.003192 0.001084 0.002712 0.000195 0.003090

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.19 6.08 -7.90 -2.69 -9.83 0.12 4.64 -0.24 -0.33 -3.92 -6.05 6.33 -7.16 -0.58 -10.10
Bud Neck 8.90 9.76 8.04 -1.52 -2.25 -2.25 8.37 2.74 3.75 -5.86 5.86 13.74 7.81 0.90 -6.95
Bud Site -1.25 -3.72 -12.11 -11.52 -7.03 -0.30 -2.23 -7.90 -6.82 -6.44 -0.81 -4.34 -14.28 -13.47 -10.73
Cell Periphery 9.50 4.18 7.34 -4.37 4.94 0.74 6.69 7.68 3.33 2.17 9.11 9.38 10.08 -1.13 1.69
Cytoplasm 26.74 14.69 16.20 -7.49 -1.22 9.21 -5.50 9.92 -0.74 13.91 28.07 -0.01 18.23 -7.99 13.78
Cytoplasmic Foci -12.40 36.68 9.57 20.18 -21.12 -1.09 24.38 10.97 8.80 -10.82 -10.10 31.90 14.30 21.45 -15.67
Eisosomes 12.05 16.24 15.40 4.85 -6.40 -0.99 11.79 11.50 5.94 -2.33 9.97 19.14 18.31 7.84 -6.55
Endoplasmic Reticulum 1.60 -11.88 -5.46 -6.54 7.04 2.45 -0.99 2.70 -0.81 4.90 2.65 -7.96 -3.34 -5.43 3.97
Endosome -13.45 5.88 -3.75 9.23 -7.13 -6.20 7.60 -6.32 1.31 -11.12 -13.95 8.59 -7.42 6.80 -12.69
Golgi -4.06 27.57 23.18 21.22 -7.43 -3.46 21.14 17.67 12.08 -4.66 -5.67 34.01 28.55 24.46 -7.86
Lipid Particles -2.75 25.56 11.80 12.50 -10.33 0.22 19.89 10.05 6.43 -8.15 -2.65 30.43 15.41 14.39 -12.05
Mitochondria 2.30 9.90 8.86 9.15 -2.44 -3.36 8.99 6.79 5.47 -4.78 -0.92 13.01 11.03 10.41 -4.96
Mitotic Spindle -4.47 -1.29 -7.28 -5.19 -5.05 -0.35 -4.01 -9.42 -9.40 -7.77 -4.46 -4.20 -11.77 -9.96 -9.06
None 0.11 3.88 4.12 1.91 1.21 2.95 0.47 1.35 -2.40 0.62 0.91 0.90 3.23 0.96 1.24
Nuclear Periphery 1.14 -16.74 -8.09 -9.02 11.82 0.58 -10.45 -5.83 -5.67 1.24 1.40 -17.35 -7.94 -9.15 6.17
Nuclear Periphery Foci -3.04 -13.18 -11.17 -10.63 0.65 1.85 -9.32 -8.32 -8.66 0.16 -0.81 -12.87 -13.66 -13.44 -0.02
Nucleolus 1.00 1.65 -0.46 -1.85 -2.72 1.64 -0.78 -3.98 -4.64 -3.79 1.70 -1.04 -4.01 -4.71 -3.58
Nucleus 10.24 -17.29 -7.78 -12.27 14.73 10.07 -21.39 -5.49 -11.36 15.82 14.96 -27.88 -9.18 -16.91 21.91
Peroxisomes -10.38 22.67 3.35 12.14 -13.58 -4.09 13.19 0.82 4.41 -9.91 -11.56 21.41 2.83 12.83 -14.49
Vacuole -2.29 -25.90 -27.85 -27.01 7.87 -3.37 -25.10 -22.55 -18.86 0.76 -3.20 -33.29 -35.52 -33.63 2.66
Vacuole Periphery -1.53 9.04 -1.29 -1.03 -2.32 -3.26 8.15 -1.51 2.37 -2.62 -3.54 12.54 -1.98 0.64 -3.54
Standard name
Human Ortholog
Description Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
Localization
Cell Percentages ER (34%), cytoplasm (3%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mtc4

Mtc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available