Standard name
Human Ortholog
Description AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 – 0.89 0.96 0.87 0.77 – 0.67 0.73 0.7 0.71 0.96 0.98 0.97 0.95 0.96 0.98 0.91 0.93 0.92 0.9 0.88 0.9
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.11 0.1 – 0.32 0.2 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0.05 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 – 0.11 0 0 0.25 – 0.13 0.1 0.05 0.06 0 0 0.09 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud 0 1 – 1 0 2 1 – 3 3 2 0 1 0 0 0 0 0 0 0 0 1 5 8
Bud Neck 0 0 – 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 – 0 0 0 1 – 1 4 1 1 2 1 2 7 4 5 0 0 0 0 0 0
Cytoplasm 283 314 – 32 65 78 188 – 123 124 105 137 341 495 489 190 122 152 276 301 58 191 495 385
Endoplasmic Reticulum 2 2 – 0 1 0 0 – 2 2 0 4 1 2 1 9 6 5 3 2 0 1 10 4
Endosome 1 0 – 0 0 0 0 – 0 3 0 1 2 1 0 0 0 0 8 2 0 1 7 4
Golgi 2 0 – 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 5 0 0 0 2 0
Mitochondria 0 1 – 0 0 10 24 – 59 34 42 49 0 0 0 1 0 0 3 3 0 0 5 2
Nucleus 1 0 – 0 1 2 2 – 2 9 12 15 5 6 2 8 4 2 0 2 1 0 2 2
Nuclear Periphery 0 0 – 0 0 0 1 – 2 3 1 0 0 1 0 0 0 0 0 1 0 0 2 0
Nucleolus 0 0 – 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 2 0 3 4 4
Vac/Vac Membrane 16 2 – 4 1 3 61 – 24 17 7 12 9 9 44 8 4 2 1 3 1 8 12 7
Unique Cell Count 296 319 36 68 90 245 183 169 149 194 354 505 506 199 127 155 305 324 64 213 563 427
Labelled Cell Count 312 320 37 68 97 279 216 199 171 219 362 516 538 223 140 166 305 324 64 213 563 427


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 8.5 – 7.3 7.9 4.7 6.2 – 5.3 5.4 5.3 5.2 9.3 8.3 8.9 11.2 11.2 11.9 8.4 8.4 8.9
Std Deviation (1e-4) 2.0 1.5 – 0.9 1.2 1.1 1.4 – 1.4 1.2 1.0 0.9 1.8 1.7 1.9 2.1 2.2 2.5 2.0 1.9 2.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5037 1.0116 0.6162 0.2101 0.2172 1.0602 2.6129 3.0595 2.8758 2.4186 2.2656 2.8658 4.2347 5.6886 4.9617 4.5651 4.9213 4.9309
Actin 0.0712 0.0005 0.0025 0.0532 0.0697 0.0135 0.0144 0.0016 0.0091 0.0074 0.0217 0.0014 0.0091 0.0002 0.0122 0.0011 0.0009 0.0031
Bud 0.0016 0.0004 0.0002 0.0003 0.0033 0.0005 0.0022 0.0193 0.012 0.0243 0.002 0.0029 0.0002 0.0001 0.0003 0.0002 0.0002 0.0004
Bud Neck 0.0019 0.0001 0.002 0.0006 0.001 0.0006 0.0034 0.0003 0.0017 0.0003 0.0003 0.002 0.0004 0.0001 0.0002 0.0002 0.0002 0.0009
Bud Periphery 0.002 0.0002 0.0001 0.0002 0.0042 0.0003 0.0021 0.0078 0.0041 0.0094 0.0025 0.001 0.0002 0 0.0004 0.0001 0.0001 0.0005
Bud Site 0.0094 0.0008 0.0047 0.002 0.0069 0.0002 0.0148 0.0206 0.0183 0.0018 0.0034 0.0011 0.0014 0.0004 0.0004 0.0003 0.0003 0.0005
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0004 0 0.0005 0.0004 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.5803 0.9141 0.8342 0.7559 0.4951 0.8941 0.5884 0.7796 0.7237 0.7091 0.6513 0.8803 0.6635 0.7787 0.7157 0.8686 0.7554 0.8149
Cytoplasmic Foci 0.0379 0.0071 0.018 0.0283 0.0254 0.0112 0.0403 0.0117 0.028 0.0457 0.0185 0.0164 0.0109 0.0056 0.0182 0.0114 0.0077 0.007
Eisosomes 0.0003 0 0 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0198 0.0053 0.0143 0.008 0.017 0.0056 0.0062 0.0032 0.0041 0.0072 0.0026 0.0032 0.0081 0.0033 0.0045 0.0071 0.004 0.0021
Endosome 0.0473 0.0074 0.0114 0.0573 0.1092 0.0136 0.0453 0.0104 0.0178 0.016 0.0093 0.0082 0.0082 0.0071 0.0093 0.008 0.0154 0.0036
Golgi 0.0255 0.0005 0.0026 0.0084 0.0435 0.0031 0.0101 0.0034 0.0084 0.0028 0.0072 0.0008 0.0008 0.0002 0.0009 0.0004 0.0008 0.0003
Lipid Particles 0.011 0.0002 0.0009 0.0112 0.0144 0.0019 0.0172 0.0003 0.0026 0.0033 0.0294 0.0001 0.0011 0.0003 0.0009 0.0001 0.001 0.0004
Mitochondria 0.0102 0.0002 0.0003 0.0018 0.0392 0.0019 0.0199 0.0018 0.0156 0.0084 0.0468 0.0013 0.0003 0.0001 0.0004 0.0003 0.0005 0.0002
None 0.1202 0.0566 0.0937 0.0502 0.1059 0.039 0.146 0.1324 0.1238 0.1206 0.1659 0.0636 0.2838 0.1987 0.226 0.0948 0.1935 0.1601
Nuclear Periphery 0.014 0.0017 0.0036 0.0039 0.0288 0.0043 0.0123 0.0009 0.0029 0.0048 0.001 0.0016 0.0029 0.0009 0.0015 0.0017 0.0079 0.0006
Nucleolus 0.0005 0 0.0001 0.001 0.0009 0.0001 0.0044 0.0003 0.001 0.0008 0.0011 0.0002 0.0002 0 0.0001 0 0.0005 0.0001
Nucleus 0.0041 0.0017 0.0021 0.0033 0.0066 0.0051 0.0278 0.0023 0.0144 0.0054 0.0012 0.0077 0.0019 0.0019 0.002 0.0029 0.0048 0.0018
Peroxisomes 0.0157 0.0001 0.0044 0.0033 0.0042 0.0014 0.0107 0.0002 0.0053 0.0078 0.0315 0.0024 0.0002 0 0.0007 0.0001 0.0002 0.0007
Punctate Nuclear 0.0186 0.0004 0.0008 0.0024 0.0032 0.0012 0.0178 0.0011 0.0045 0.0201 0.0016 0.0034 0.0048 0.0004 0.0039 0.0006 0.0043 0.0018
Vacuole 0.0053 0.0022 0.0034 0.0071 0.0121 0.002 0.0132 0.002 0.0018 0.0033 0.0016 0.002 0.0014 0.0017 0.0019 0.0015 0.0019 0.0008
Vacuole Periphery 0.0029 0.0004 0.0005 0.0013 0.0084 0.0007 0.003 0.0003 0.0005 0.0014 0.0007 0.0004 0.0002 0.0002 0.0002 0.0003 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.5135 59.2672 58.6385 50.9129 66.5163 52.0632 53.8612 51.5197 59.3151 50.3541
Translational Efficiency 2.2373 1.7445 1.8561 2.1747 1.5563 1.8606 1.6557 2.0979 1.6704 1.759

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2546 1097 2470 1984 1668 1752 1142 1473 4214 2849 3612 3457

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 846.80 828.56 1094.97 970.96 771.18 923.60 976.19 938.33 816.87 887.01 1057.42 957.06
Standard Deviation 109.42 123.46 157.79 159.14 117.66 123.34 144.60 151.91 118.66 131.77 163.36 156.93
Intensity Change Log 2 — -0.031415 0.370798 0.197391 — 0.260201 0.340094 0.283028 — 0.114941 0.356245 0.238842

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000134 0.000273 0.000053 0.000260 0.000109 0.000326 0.000340 0.000230 0.000124 0.000305 0.000144 0.000247
Bud Neck 0.006887 0.004747 0.001362 0.006020 0.002850 0.008513 0.000955 0.005966 0.005289 0.007063 0.001233 0.005997
Bud Site 0.008637 0.015396 0.006204 0.022175 0.004059 0.020373 0.003107 0.025617 0.006825 0.018457 0.005225 0.023642
Cell Periphery 0.000103 0.000116 0.000029 0.000082 0.000095 0.000097 0.000091 0.000096 0.000100 0.000104 0.000048 0.000088
Cytoplasm 0.678160* 0.703177* 0.773502* 0.654792* 0.706892* 0.705483* 0.675531* 0.654850* 0.689533* 0.704595* 0.742526* 0.654817*
Cytoplasmic Foci 0.150616 0.128518 0.007053 0.012009 0.122508 0.137440 0.013312 0.019162 0.139490 0.134005 0.009032 0.015057
Eisosomes 0.000055 0.000031 0.000004 0.000016 0.000037 0.000038 0.000019 0.000023 0.000048 0.000035 0.000009 0.000019
Endoplasmic Reticulum 0.001097 0.001318 0.000393 0.000982 0.001362 0.000539 0.001532 0.000990 0.001202 0.000839 0.000753 0.000985
Endosome 0.005431 0.011140 0.000566 0.003272 0.005323 0.008389 0.002064 0.004365 0.005388 0.009448 0.001040 0.003737
Golgi 0.005523 0.004475 0.000054 0.003965 0.001745 0.002879 0.000853 0.003796 0.004027 0.003493 0.000307 0.003893
Lipid Particles 0.002620 0.002515 0.000220 0.000523 0.002222 0.001395 0.000739 0.000745 0.002463 0.001826 0.000384 0.000618
Mitochondria 0.002656 0.002374 0.000195 0.001896 0.002287 0.001473 0.000204 0.001864 0.002510 0.001820 0.000198 0.001883
Mitotic Spindle 0.002682 0.001561 0.002275 0.047080 0.001396 0.006801 0.004959 0.027628 0.002173 0.004784 0.003123 0.038792
None 0.011271 0.006468 0.005779 0.007476 0.007155 0.003634 0.008947 0.007286 0.009642 0.004726 0.006781 0.007395
Nuclear Periphery 0.000127 0.000191 0.000464 0.001214 0.000283 0.000081 0.000709 0.000853 0.000189 0.000123 0.000542 0.001060
Nuclear Periphery Foci 0.000566 0.001978 0.000929 0.001351 0.001148 0.000575 0.003682 0.001734 0.000797 0.001115 0.001799 0.001514
Nucleolus 0.000769 0.000906 0.000119 0.000536 0.001039 0.000453 0.000197 0.000589 0.000876 0.000627 0.000144 0.000558
Nucleus 0.071805 0.043916 0.176342* 0.156427* 0.094621 0.044107 0.218620* 0.145362* 0.080836 0.044033 0.189709* 0.151712*
Peroxisomes 0.001555 0.002489 0.000072 0.000547 0.000924 0.003712 0.000165 0.000785 0.001305 0.003241 0.000101 0.000648
Vacuole 0.047677 0.065474 0.024308 0.077514 0.042821 0.052453 0.063756 0.095679 0.045755 0.057467 0.036780 0.085254
Vacuole Periphery 0.001628 0.002937 0.000078 0.001864 0.001123 0.001238 0.000219 0.002379 0.001428 0.001892 0.000122 0.002083

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.47 4.05 -3.47 0.95 -7.32 -2.69 -2.60 -2.60 1.59 1.62 -3.46 -1.09 -4.30 1.79 -1.77
Bud Neck 3.18 10.95 4.25 0.58 -6.90 -6.71 3.81 -3.10 3.25 -5.35 -2.88 11.41 1.28 3.73 -8.45
Bud Site -4.51 2.64 -1.40 2.83 -3.40 -9.02 0.88 -5.36 1.99 -5.74 -9.13 2.40 -4.82 3.76 -6.05
Cell Periphery -1.79 21.04 9.30 7.11 -10.21 -0.22 0.10 2.38 3.27 2.16 -0.89 11.87 7.48 7.67 -5.00
Cytoplasm -2.63 -8.41 19.29 17.19 26.76 0.07 12.81 16.25 15.69 2.72 -2.54 1.00 25.05 24.22 23.20
Cytoplasmic Foci 3.86 42.60 42.27 24.84 -1.38 -2.87 30.40 29.86 30.27 -0.64 1.45 52.42 51.41 39.79 -2.34
Eisosomes 7.39 20.27 12.18 3.47 -20.52 -0.19 4.53 5.08 5.26 -0.63 6.04 20.72 13.06 6.87 -14.42
Endoplasmic Reticulum -1.22 4.16 -1.71 -0.06 -10.46 4.82 -3.22 0.48 -7.09 4.72 2.96 1.64 -1.00 -5.49 -4.01
Endosome -6.11 16.33 11.21 10.56 -4.58 -4.44 8.71 5.30 8.09 -1.79 -7.38 18.77 11.46 13.78 -4.63
Golgi 1.32 11.92 7.14 4.08 -2.45 -2.55 2.85 0.18 1.79 -1.63 1.25 11.87 5.66 4.43 -2.98
Lipid Particles 0.24 13.87 10.51 3.59 -13.29 3.13 6.93 8.54 2.95 2.10 2.55 15.92 13.46 4.61 -7.43
Mitochondria 0.35 6.31 3.96 1.63 -4.63 1.27 3.63 2.02 1.11 -3.91 1.44 7.07 4.26 2.02 -6.05
Mitotic Spindle 1.31 -0.84 -9.10 -9.53 -8.91 -3.70 -3.42 -6.65 -4.12 -4.61 -2.67 -2.94 -11.23 -9.03 -10.03
None 3.65 6.76 4.82 -0.44 -3.24 4.55 0.01 0.75 -4.67 0.92 6.76 5.97 4.50 -3.68 -1.86
Nuclear Periphery -1.88 -18.44 -16.16 -15.17 -11.09 4.21 -10.88 -10.89 -14.89 -1.91 2.71 -18.87 -19.54 -20.75 -11.08
Nuclear Periphery Foci -7.61 -5.64 -5.57 2.70 -1.23 5.71 -10.47 -1.50 -3.77 7.59 -3.52 -11.20 -4.64 -2.20 4.61
Nucleolus -1.06 11.20 3.71 3.86 -4.09 2.91 4.10 2.52 -0.61 -3.54 2.73 8.19 4.10 2.18 -5.56
Nucleus 10.56 -33.47 -32.08 -36.09 -5.27 14.84 -20.73 -16.54 -28.01 5.66 18.57 -37.63 -35.08 -46.13 -0.54
Peroxisomes -1.94 12.48 9.51 4.98 -2.38 -4.92 4.32 2.62 5.95 -1.59 -4.95 12.26 8.61 7.67 -2.93
Vacuole -5.43 -0.23 -25.13 -18.45 -23.78 -3.21 -14.90 -21.51 -19.79 -5.51 -5.71 -10.74 -33.05 -28.19 -22.70
Vacuole Periphery -2.46 5.55 3.02 4.79 -2.46 -0.52 6.94 0.14 0.39 -2.15 -1.69 7.49 2.31 3.73 -3.18
Standard name
Human Ortholog
Description AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tae1

Tae1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tae1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available