Standard name | |
---|---|
Human Ortholog | |
Description | Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 1 | 3 | 2 | 9 | 19 | 15 | 4 | 8 | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 4 | 9 | 3 | 1 | 6 | 6 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 4 | 7 | 17 | 10 | 27 | 45 | 42 | 29 | 20 | 25 | 44 | 4 | 16 | 9 | 10 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 220 | 295 | 321 | 238 | 284 | 481 | 486 | 455 | 301 | 310 | 251 | 331 | 179 | 342 | 314 | 281 | 302 | 267 | 208 | 285 | 315 | 221 | 230 | 291 |
Endoplasmic Reticulum | 4 | 6 | 24 | 8 | 7 | 12 | 20 | 52 | 14 | 29 | 18 | 14 | 4 | 2 | 2 | 13 | 16 | 8 | 5 | 4 | 23 | 2 | 6 | 10 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 6 | 0 | 1 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 2 | 4 | 11 | 95 | 201 | 125 | 86 | 207 | 150 | 144 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 1 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 2 | 2 | 1 | 4 | 0 | 1 | 1 | 3 | 2 | 0 | 0 | 1 | 1 | 4 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 1 | 19 | 6 | 3 | 13 | 27 | 24 | 7 | 13 | 20 | 0 | 4 | 1 | 3 | 10 | 6 | 0 | 3 | 4 | 4 | 5 | 4 |
Unique Cell Count | 226 | 300 | 345 | 268 | 302 | 542 | 665 | 631 | 398 | 480 | 379 | 479 | 181 | 347 | 316 | 288 | 313 | 276 | 219 | 301 | 355 | 235 | 251 | 313 |
Labelled Cell Count | 229 | 305 | 358 | 296 | 321 | 634 | 804 | 727 | 468 | 589 | 468 | 565 | 187 | 366 | 328 | 313 | 335 | 292 | 219 | 301 | 355 | 235 | 251 | 313 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.2 | 18.9 | 14.5 | 13.9 | 11.3 | 9.2 | 8.7 | 9.0 | 9.2 | 7.6 | 7.5 | 8.1 | 18.3 | 17.0 | 15.7 | 21.8 | 19.4 | 21.4 | 18.8 | 18.8 | 20.3 |
Std Deviation (1e-4) | 2.7 | 3.6 | 2.7 | 4.3 | 2.1 | 2.0 | 3.1 | 2.6 | 5.6 | 1.8 | 1.9 | 3.9 | 3.8 | 3.5 | 3.3 | 5.6 | 4.2 | 4.6 | 4.1 | 4.4 | 4.2 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.37 | -0.66 | -0.75 | -0.69 | -0.66 | -0.93 | -0.96 | -0.84 | 0.33 | 0.22 | 0.11 | 0.58 | 0.42 | 0.56 | 0.37 | 0.37 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 2.8 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.7 | 1.0 | 2.2 | 3.2 | 3.2 | 3.3 | 1.7 | 3.0 | 4.2 | 0 | 1.9 | 0.7 | 1.1 | 0 | 0.7 |
Cytoplasm | -1.8 | 0.5 | -2.1 | -7.5 | -7.7 | -6.4 | -9.5 | -8.8 | -8.3 | 2.9 | 3.6 | 4.2 | 2.6 | 2.0 | 2.0 |
Endoplasmic Reticulum | -2.2 | -2.8 | -3.5 | -2.9 | 0.7 | -2.1 | -0.5 | -1.3 | -2.7 | -2.3 | -4.4 | -4.2 | -1.3 | -1.0 | -2.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.8 | 7.9 | 11.1 | 8.5 | 8.8 | 13.9 | 12.9 | 10.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.7 | 0 | 0 | 0 | 3.6 | 4.3 | 0 | 3.1 | 3.5 | 0 | 0 | 0 | 0 | 2.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.5744 | 14.5445 | 12.9989 | 10.7998 | 10.9946 | 12.9752 | 10.693 | 15.627 | 14.5393 | 13.1512 | 12.0621 | 13.9782 | 11.6088 | 14.9835 | 13.2966 | 14.4813 | 12.1968 | 13.9206 |
Actin | 0.0185 | 0.0021 | 0.0108 | 0.0088 | 0.0276 | 0.0018 | 0.038 | 0.0012 | 0.0016 | 0.0083 | 0.0126 | 0.0116 | 0.024 | 0.0127 | 0.0581 | 0.0001 | 0.0079 | 0.0008 |
Bud | 0.0011 | 0.0008 | 0.0017 | 0.0009 | 0.0011 | 0.0008 | 0.0011 | 0.0004 | 0.0005 | 0.0035 | 0.003 | 0.0008 | 0.001 | 0.0005 | 0.0009 | 0.0013 | 0.0048 | 0.0006 |
Bud Neck | 0.0015 | 0.0005 | 0.0012 | 0.001 | 0.0011 | 0.0015 | 0.0008 | 0.0002 | 0.0004 | 0.0007 | 0.0011 | 0.001 | 0.0011 | 0.0003 | 0.0028 | 0.0004 | 0.0018 | 0.0012 |
Bud Periphery | 0.0012 | 0.0003 | 0.0019 | 0.0008 | 0.0017 | 0.0025 | 0.0012 | 0.0001 | 0.0002 | 0.003 | 0.0095 | 0.0006 | 0.0012 | 0.0002 | 0.0006 | 0.0004 | 0.0046 | 0.0003 |
Bud Site | 0.0027 | 0.012 | 0.0015 | 0.0009 | 0.0016 | 0.0003 | 0.0026 | 0.0006 | 0.001 | 0.0049 | 0.0065 | 0.0003 | 0.0071 | 0.0018 | 0.006 | 0.0002 | 0.0061 | 0.0001 |
Cell Periphery | 0.0029 | 0.0011 | 0.0014 | 0.0005 | 0.0006 | 0.0015 | 0.0011 | 0.0002 | 0.0001 | 0.0012 | 0.0041 | 0.0001 | 0.0012 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0001 |
Cytoplasm | 0.6933 | 0.9212 | 0.7876 | 0.4765 | 0.6296 | 0.8247 | 0.6676 | 0.9293 | 0.8758 | 0.6788 | 0.6904 | 0.7388 | 0.7061 | 0.9611 | 0.8258 | 0.713 | 0.6072 | 0.8211 |
Cytoplasmic Foci | 0.0171 | 0.0091 | 0.0148 | 0.0341 | 0.0294 | 0.0189 | 0.018 | 0.0107 | 0.0153 | 0.0265 | 0.0316 | 0.0232 | 0.0215 | 0.0038 | 0.0154 | 0.0162 | 0.0565 | 0.0156 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0911 | 0.0169 | 0.0327 | 0.0232 | 0.0191 | 0.0323 | 0.0772 | 0.0164 | 0.0126 | 0.0097 | 0.0121 | 0.088 | 0.0583 | 0.0052 | 0.0174 | 0.019 | 0.0078 | 0.0446 |
Endosome | 0.0641 | 0.0193 | 0.0571 | 0.1703 | 0.142 | 0.0733 | 0.0836 | 0.0158 | 0.0478 | 0.1747 | 0.1523 | 0.0892 | 0.0545 | 0.0056 | 0.0455 | 0.1087 | 0.1124 | 0.0696 |
Golgi | 0.0112 | 0.0038 | 0.0125 | 0.0307 | 0.015 | 0.0103 | 0.0172 | 0.0045 | 0.0034 | 0.0268 | 0.0323 | 0.0167 | 0.0103 | 0.001 | 0.0107 | 0.0033 | 0.0559 | 0.0043 |
Lipid Particles | 0.0085 | 0.0008 | 0.0036 | 0.0322 | 0.0378 | 0.0008 | 0.0036 | 0.0021 | 0.0007 | 0.0011 | 0.0012 | 0.0015 | 0.009 | 0.0001 | 0.0012 | 0.0001 | 0.0014 | 0 |
Mitochondria | 0.0059 | 0.0009 | 0.012 | 0.0875 | 0.0149 | 0.0092 | 0.0086 | 0.0021 | 0.0148 | 0.0112 | 0.0192 | 0.009 | 0.0164 | 0.0007 | 0.0016 | 0.0015 | 0.0987 | 0.0024 |
None | 0.0085 | 0.0005 | 0.0014 | 0.0009 | 0.0089 | 0.0015 | 0.0046 | 0.0004 | 0.0011 | 0.0005 | 0.0024 | 0.0012 | 0.0152 | 0.0003 | 0.0006 | 0.0005 | 0.0008 | 0.0005 |
Nuclear Periphery | 0.0174 | 0.0008 | 0.0047 | 0.0055 | 0.0096 | 0.0038 | 0.0158 | 0.0012 | 0.0019 | 0.0019 | 0.0021 | 0.004 | 0.0095 | 0.0004 | 0.0023 | 0.004 | 0.0008 | 0.002 |
Nucleolus | 0.001 | 0 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0052 | 0.0007 | 0.0019 | 0.0017 | 0.0056 | 0.0014 | 0.0097 | 0.0011 | 0.0013 | 0.0012 | 0.001 | 0.0022 | 0.0031 | 0.0007 | 0.0011 | 0.0015 | 0.0011 | 0.0009 |
Peroxisomes | 0.0029 | 0.0002 | 0.006 | 0.0068 | 0.0093 | 0.0002 | 0.0019 | 0.002 | 0.0003 | 0.0004 | 0.0003 | 0.0007 | 0.0031 | 0.0001 | 0.0005 | 0.0001 | 0.0048 | 0.0001 |
Punctate Nuclear | 0.0045 | 0.0001 | 0.0004 | 0.0009 | 0.0087 | 0.0004 | 0.0018 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0004 | 0.0029 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Vacuole | 0.0358 | 0.0076 | 0.0412 | 0.092 | 0.0261 | 0.0096 | 0.0367 | 0.0099 | 0.0153 | 0.0349 | 0.0119 | 0.0066 | 0.046 | 0.0045 | 0.0065 | 0.102 | 0.0167 | 0.0219 |
Vacuole Periphery | 0.0054 | 0.0012 | 0.0054 | 0.0246 | 0.0087 | 0.0049 | 0.0084 | 0.0015 | 0.0052 | 0.0104 | 0.0054 | 0.0038 | 0.0073 | 0.0006 | 0.0019 | 0.0276 | 0.0091 | 0.0137 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.0104 | 61.7528 | 50.7771 | 32.834 | 48.4133 | 39.1856 | 33.5394 | 27.9046 | 24.8216 | 36.0809 |
Translational Efficiency | 2.253 | 1.9434 | 1.9597 | 2.2908 | 1.9718 | 2.096 | 1.758 | 1.9144 | 1.6267 | 1.7328 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1033 | 145 | 559 | 1299 | 1844 | 1812 | 159 | 1206 | 2877 | 1957 | 718 | 2505 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1257.08 | 1396.74 | 1700.66 | 1778.59 | 1274.36 | 1393.86 | 1817.16 | 1827.64 | 1268.16 | 1394.07 | 1726.46 | 1802.20 |
Standard Deviation | 194.17 | 231.02 | 244.46 | 310.19 | 182.89 | 208.66 | 242.13 | 303.65 | 187.20 | 210.40 | 248.70 | 308.03 |
Intensity Change Log 2 | — | 0.151987 | 0.436018 | 0.500658 | — | 0.129313 | 0.511913 | 0.520209 | — | 0.140617 | 0.474723 | 0.510533 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000295 | 0.000661 | 0.000745 | 0.000436 | 0.000153 | 0.000195 | 0.001658 | 0.000800 | 0.000204 | 0.000230 | 0.000947 | 0.000611 |
Bud Neck | 0.000329 | 0.000754 | 0.002992 | 0.002079 | 0.000194 | 0.000297 | 0.007402 | 0.001952 | 0.000242 | 0.000331 | 0.003969 | 0.002018 |
Bud Site | 0.026062 | 0.046544 | 0.026982 | 0.029941 | 0.016733 | 0.015036 | 0.050989 | 0.025956 | 0.020082 | 0.017371 | 0.032299 | 0.028023 |
Cell Periphery | 0.000612 | 0.001093 | 0.003521 | 0.005297 | 0.000289 | 0.000270 | 0.001888 | 0.005413 | 0.000405 | 0.000331 | 0.003159 | 0.005353 |
Cytoplasm | 0.802911* | 0.783072* | 0.815155* | 0.713562* | 0.773575* | 0.833123* | 0.605950* | 0.797092* | 0.784109* | 0.829414* | 0.768827* | 0.753776* |
Cytoplasmic Foci | 0.104188 | 0.097480 | 0.025955 | 0.021212 | 0.126472 | 0.086131 | 0.077796 | 0.017520 | 0.118471 | 0.086972 | 0.037435 | 0.019435 |
Eisosomes | 0.000004 | 0.000008 | 0.000005 | 0.000004 | 0.000003 | 0.000002 | 0.000012 | 0.000006 | 0.000003 | 0.000003 | 0.000007 | 0.000005 |
Endoplasmic Reticulum | 0.006161 | 0.001488 | 0.001556 | 0.001604 | 0.005899 | 0.003168 | 0.003433 | 0.000919 | 0.005993 | 0.003043 | 0.001972 | 0.001274 |
Endosome | 0.005608 | 0.008468 | 0.005779 | 0.006353 | 0.006179 | 0.005529 | 0.019653 | 0.004604 | 0.005974 | 0.005747 | 0.008851 | 0.005511 |
Golgi | 0.016036 | 0.022137 | 0.013905 | 0.019410 | 0.018163 | 0.013673 | 0.014437 | 0.015149 | 0.017399 | 0.014300 | 0.014023 | 0.017359 |
Lipid Particles | 0.001298 | 0.005424 | 0.011788 | 0.010945 | 0.000661 | 0.000872 | 0.016484 | 0.013550 | 0.000890 | 0.001210 | 0.012828 | 0.012199 |
Mitochondria | 0.001502 | 0.000837 | 0.000028 | 0.000345 | 0.000411 | 0.000386 | 0.000016 | 0.000852 | 0.000803 | 0.000420 | 0.000025 | 0.000589 |
Mitotic Spindle | 0.000516 | 0.002154 | 0.001932 | 0.003563 | 0.001136 | 0.001220 | 0.004462 | 0.002328 | 0.000914 | 0.001289 | 0.002492 | 0.002968 |
None | 0.000244 | 0.000299 | 0.000226 | 0.000163 | 0.000136 | 0.000169 | 0.000079 | 0.000185 | 0.000175 | 0.000179 | 0.000193 | 0.000173 |
Nuclear Periphery | 0.000018 | 0.000020 | 0.000070 | 0.000168 | 0.000017 | 0.000034 | 0.000195 | 0.000051 | 0.000017 | 0.000033 | 0.000097 | 0.000112 |
Nuclear Periphery Foci | 0.000800 | 0.000861 | 0.006601 | 0.003184 | 0.000523 | 0.000523 | 0.012660 | 0.002410 | 0.000622 | 0.000548 | 0.007943 | 0.002811 |
Nucleolus | 0.000138 | 0.000467 | 0.000052 | 0.000186 | 0.000017 | 0.000024 | 0.000052 | 0.000138 | 0.000061 | 0.000057 | 0.000052 | 0.000162 |
Nucleus | 0.001565 | 0.001964 | 0.004348 | 0.003485 | 0.000877 | 0.001485 | 0.004589 | 0.003119 | 0.001124 | 0.001520 | 0.004401 | 0.003309 |
Peroxisomes | 0.000228 | 0.000824 | 0.000262 | 0.000421 | 0.000129 | 0.000250 | 0.000700 | 0.000555 | 0.000165 | 0.000293 | 0.000359 | 0.000486 |
Vacuole | 0.014573 | 0.016358 | 0.073031 | 0.154809 | 0.018119 | 0.026215 | 0.172508 | 0.098031 | 0.016846 | 0.025484 | 0.095060 | 0.127474 |
Vacuole Periphery | 0.016911 | 0.009088 | 0.005067 | 0.022833 | 0.030313 | 0.011398 | 0.005037 | 0.009370 | 0.025501 | 0.011227 | 0.005061 | 0.016351 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.27 | -4.17 | -3.61 | 0.75 | 2.89 | -1.97 | -6.12 | -2.44 | -2.28 | 2.36 | -0.86 | -7.31 | -3.18 | -2.93 | 2.17 |
Bud Neck | -3.82 | -10.03 | -12.81 | -8.47 | 2.63 | -4.27 | -8.67 | -12.09 | -11.35 | 6.35 | -3.66 | -13.11 | -17.85 | -16.81 | 6.11 |
Bud Site | -3.49 | -0.77 | -1.80 | 2.75 | -0.64 | 1.53 | -5.56 | -5.05 | -6.04 | 3.93 | 2.43 | -5.41 | -5.76 | -7.48 | 1.95 |
Cell Periphery | -1.95 | -7.04 | -8.50 | -6.86 | -2.24 | 0.70 | -5.66 | -9.32 | -9.35 | -5.66 | 2.10 | -8.42 | -12.74 | -12.93 | -3.97 |
Cytoplasm | 0.92 | -0.46 | 9.23 | 3.56 | 8.39 | -7.80 | 7.76 | -1.57 | 5.20 | -8.26 | -6.83 | 2.10 | 5.48 | 11.68 | 1.50 |
Cytoplasmic Foci | 0.62 | 16.36 | 18.42 | 7.33 | 2.39 | 8.72 | 7.07 | 28.48 | 21.32 | 9.69 | 7.97 | 23.01 | 33.87 | 22.80 | 7.88 |
Eisosomes | -1.14 | -1.98 | -0.48 | 1.07 | 1.86 | 1.06 | -7.60 | -3.51 | -3.79 | 4.09 | 1.18 | -7.26 | -3.89 | -4.31 | 2.74 |
Endoplasmic Reticulum | 4.11 | 4.48 | 4.56 | -0.03 | 0.14 | 3.42 | 2.53 | 7.09 | 4.15 | 2.57 | 4.22 | 6.65 | 8.29 | 3.87 | 2.13 |
Endosome | -1.61 | -0.47 | -0.79 | 1.26 | -0.30 | 1.37 | -6.79 | 2.83 | 1.66 | 7.52 | 0.53 | -4.19 | 1.26 | 0.68 | 4.66 |
Golgi | -1.51 | 1.50 | -1.15 | 0.94 | -2.17 | 4.96 | 2.79 | 2.51 | -0.40 | -0.41 | 3.66 | 3.24 | 0.98 | -1.57 | -1.74 |
Lipid Particles | -1.12 | -11.19 | -11.84 | -1.45 | 1.02 | -2.17 | -5.57 | -11.60 | -11.42 | 0.94 | -1.11 | -12.43 | -16.57 | -14.99 | 0.71 |
Mitochondria | 0.69 | 1.78 | 1.42 | 0.99 | -2.63 | 0.12 | 7.38 | -0.78 | -0.77 | -1.61 | 1.08 | 2.60 | 0.68 | -0.41 | -2.18 |
Mitotic Spindle | -3.39 | -4.52 | -3.43 | -1.69 | -1.86 | -0.17 | -3.60 | -2.29 | -2.25 | 0.46 | -1.12 | -4.36 | -3.82 | -3.33 | -1.64 |
None | -0.45 | 0.42 | 1.32 | 1.15 | 1.44 | -0.75 | 3.03 | -1.60 | -0.42 | -3.09 | -0.09 | -0.53 | -0.17 | -0.02 | 0.34 |
Nuclear Periphery | -0.29 | -6.51 | -1.96 | -1.92 | -1.20 | -0.98 | -2.73 | -4.00 | -1.11 | 2.12 | -0.98 | -5.39 | -2.42 | -1.88 | -0.26 |
Nuclear Periphery Foci | -0.31 | -6.57 | -9.48 | -8.86 | 3.83 | 0.02 | -3.23 | -10.09 | -11.10 | 2.69 | 0.90 | -6.89 | -14.03 | -15.75 | 4.76 |
Nucleolus | -0.72 | 0.72 | -0.43 | 0.62 | -3.79 | -3.43 | -6.52 | -4.68 | -4.41 | -3.04 | 0.07 | 0.16 | -2.17 | -2.73 | -4.90 |
Nucleus | -0.40 | -4.24 | -3.74 | -2.65 | 0.91 | -7.84 | -7.89 | -13.05 | -9.39 | 2.88 | -1.65 | -9.69 | -9.17 | -11.18 | 2.56 |
Peroxisomes | -3.00 | -1.04 | -5.42 | 1.90 | -3.93 | -3.06 | -6.95 | -6.09 | -3.85 | 1.44 | -3.22 | -6.44 | -8.49 | -3.76 | -2.76 |
Vacuole | -0.39 | -10.31 | -26.67 | -20.43 | -10.83 | -3.96 | -9.37 | -18.13 | -15.97 | 4.14 | -4.73 | -13.32 | -31.66 | -27.71 | -5.02 |
Vacuole Periphery | 2.52 | 5.78 | -1.77 | -4.33 | -10.45 | 9.59 | 13.07 | 10.69 | 2.17 | -3.73 | 9.18 | 14.20 | 6.31 | -3.59 | -10.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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