Standard name | |
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Human Ortholog | |
Description | Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 5 | 2 | 3 | 2 | 5 | 2 | 0 | 6 | 17 | 25 |
Bud | 3 | 4 | 9 | 5 | 8 | 4 | – | 15 | 7 | 17 | 9 | 15 | 3 | 2 | 3 | 2 | 1 | 1 | 1 | 1 | 0 | 1 | 5 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 |
Bud Site | 2 | 0 | 2 | 1 | 0 | 1 | – | 4 | 10 | 4 | 3 | 6 | 0 | 0 | 1 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 7 | 11 | 6 | 7 | 2 | 1 | – | 5 | 1 | 3 | 3 | 4 | 13 | 17 | 31 | 1 | 2 | 7 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 3 | 0 | 4 | 3 | 12 | 9 | – | 10 | 3 | 4 | 5 | 3 | 14 | 17 | 25 | 1 | 2 | 6 | 0 | 0 | 1 | 1 | 1 | 2 |
Endoplasmic Reticulum | 3 | 0 | 3 | 0 | 0 | 4 | – | 1 | 3 | 0 | 2 | 0 | 4 | 8 | 6 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 4 |
Endosome | 11 | 0 | 0 | 5 | 27 | 2 | – | 8 | 1 | 2 | 1 | 0 | 18 | 29 | 35 | 1 | 5 | 5 | 20 | 4 | 8 | 12 | 35 | 49 |
Golgi | 8 | 6 | 0 | 0 | 3 | 0 | – | 0 | 0 | 1 | 1 | 0 | 29 | 67 | 136 | 4 | 12 | 18 | 22 | 13 | 2 | 15 | 62 | 76 |
Mitochondria | 386 | 144 | 135 | 246 | 353 | 328 | – | 499 | 426 | 578 | 325 | 457 | 512 | 740 | 756 | 0 | 6 | 5 | 350 | 134 | 117 | 153 | 425 | 478 |
Nucleus | 3 | 1 | 0 | 0 | 2 | 3 | – | 3 | 3 | 2 | 3 | 1 | 3 | 2 | 5 | 2 | 4 | 3 | 1 | 0 | 0 | 0 | 2 | 3 |
Nuclear Periphery | 17 | 3 | 3 | 8 | 12 | 38 | – | 70 | 62 | 55 | 45 | 40 | 19 | 30 | 30 | 3 | 5 | 3 | 5 | 0 | 1 | 2 | 4 | 5 |
Nucleolus | 11 | 5 | 3 | 9 | 16 | 33 | – | 59 | 45 | 66 | 31 | 37 | 16 | 25 | 17 | 1 | 3 | 7 | 0 | 0 | 0 | 0 | 3 | 3 |
Peroxisomes | 6 | 1 | 0 | 5 | 16 | 0 | – | 17 | 4 | 9 | 0 | 2 | 12 | 39 | 37 | 2 | 0 | 7 | 12 | 5 | 4 | 11 | 32 | 35 |
SpindlePole | 2 | 0 | 0 | 2 | 2 | 0 | – | 8 | 5 | 15 | 4 | 6 | 7 | 7 | 5 | 0 | 2 | 5 | 1 | 0 | 0 | 3 | 2 | 7 |
Vac/Vac Membrane | 74 | 33 | 7 | 4 | 28 | 25 | – | 20 | 13 | 14 | 8 | 12 | 84 | 86 | 109 | 4 | 13 | 13 | 18 | 4 | 0 | 11 | 34 | 60 |
Unique Cell Count | 424 | 161 | 141 | 255 | 392 | 357 | 554 | 454 | 619 | 350 | 473 | 580 | 838 | 925 | 21 | 48 | 70 | 443 | 170 | 139 | 229 | 642 | 770 | |
Labelled Cell Count | 541 | 208 | 172 | 295 | 482 | 448 | 719 | 583 | 770 | 440 | 583 | 737 | 1074 | 1201 | 24 | 61 | 85 | 443 | 170 | 139 | 229 | 642 | 770 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 8.1 | 4.7 | 5.8 | 6.9 | 6.4 | – | 7.4 | 6.8 | 7.2 | 7.1 | 7.4 | 7.6 | 8.4 | 9.1 | 9.0 | 9.0 | 10.0 | 7.4 | 7.9 | 7.8 |
Std Deviation (1e-4) | 1.1 | 1.3 | 1.0 | 2.3 | 1.7 | 1.8 | – | 2.1 | 2.4 | 2.2 | 2.6 | 2.6 | 1.3 | 1.6 | 1.6 | 2.1 | 2.3 | 2.9 | 3.2 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.3 | 0.56 | 0.45 | – | 0.65 | 0.53 | 0.61 | 0.59 | 0.65 | 0.68 | 0.83 | 0.95 | 0.94 | 0.94 | 1.09 | 0.64 | 0.74 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.0 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.5 | 4.9 | 0 | 0 | 6.3 |
Mitochondria | 0.4 | -2.1 | -1.5 | – | -2.1 | -0.9 | -1.1 | -1.2 | 0.5 | -2.6 | -2.7 | -4.2 | -11.0 | -11.4 | -12.8 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 3.1 | – | 3.6 | 3.8 | 2.7 | 3.6 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1.1 | 2.8 | – | 3.2 | 3.0 | 3.2 | 2.7 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0.9 | 0.8 | – | -0.7 | 0 | 0 | 0 | 0 | 3.1 | 2.0 | 2.4 | 0 | 4.3 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.8229 | 4.9839 | 4.9988 | 4.4987 | 4.0947 | 4.9248 | 3.3928 | 3.9224 | 3.636 | 3.2116 | 3.0831 | 4.1086 | 5.7465 | 5.9869 | 6.0353 | 5.799 | 5.538 | 6.3184 |
Actin | 0.0118 | 0.0043 | 0.0013 | 0.0005 | 0.0245 | 0.0014 | 0.0302 | 0.0109 | 0.0044 | 0.0102 | 0.0132 | 0.0107 | 0.0059 | 0.0025 | 0.0078 | 0.0003 | 0.0063 | 0.0013 |
Bud | 0.0002 | 0.0009 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0008 | 0.0032 | 0.0005 | 0.0006 | 0.0038 | 0.0004 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0076 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0001 |
Bud Periphery | 0.0004 | 0.0013 | 0.0011 | 0.0014 | 0.0005 | 0.0002 | 0.0015 | 0.0077 | 0.0012 | 0.0018 | 0.0143 | 0.0012 | 0.0008 | 0.0011 | 0.0006 | 0.0003 | 0.0003 | 0.0002 |
Bud Site | 0.0006 | 0.0052 | 0.0002 | 0.0007 | 0.0004 | 0.0001 | 0.0098 | 0.0117 | 0.0062 | 0.0019 | 0.0011 | 0.0004 | 0.0041 | 0.0029 | 0.0006 | 0.0001 | 0.0002 | 0.0001 |
Cell Periphery | 0.001 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0.0022 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0009 | 0.0013 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0011 | 0.0008 | 0.0007 | 0.0003 | 0.0105 | 0.0003 | 0.0011 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0063 | 0.0209 | 0.0027 | 0.003 | 0.021 | 0.0047 | 0.0209 | 0.0176 | 0.0059 | 0.0128 | 0.0166 | 0.0089 | 0.0044 | 0.0078 | 0.0053 | 0.0009 | 0.0061 | 0.0021 |
Eisosomes | 0.0032 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0006 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0008 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0011 | 0.0006 | 0.0009 | 0.0003 | 0.0024 | 0.0009 | 0.0013 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endosome | 0.0202 | 0.0334 | 0.0142 | 0.0228 | 0.025 | 0.0087 | 0.0454 | 0.0357 | 0.0245 | 0.0219 | 0.0616 | 0.0135 | 0.0188 | 0.0296 | 0.0119 | 0.0041 | 0.0511 | 0.0094 |
Golgi | 0.0159 | 0.0314 | 0.021 | 0.0222 | 0.0468 | 0.0137 | 0.0392 | 0.0413 | 0.0207 | 0.0306 | 0.0321 | 0.0228 | 0.02 | 0.033 | 0.0207 | 0.0046 | 0.0337 | 0.0115 |
Lipid Particles | 0.0217 | 0.0057 | 0.0055 | 0.0129 | 0.0252 | 0.0039 | 0.023 | 0.0067 | 0.006 | 0.0209 | 0.0288 | 0.0129 | 0.0311 | 0.0086 | 0.0056 | 0.0011 | 0.0037 | 0.0042 |
Mitochondria | 0.8904 | 0.8339 | 0.9339 | 0.9227 | 0.8347 | 0.9564 | 0.7655 | 0.8045 | 0.9051 | 0.8461 | 0.7558 | 0.9041 | 0.8887 | 0.8652 | 0.9229 | 0.9786 | 0.8725 | 0.9606 |
None | 0.0033 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0 | 0.0012 | 0.0002 | 0.0006 | 0.0004 | 0.0027 | 0.0003 | 0.0015 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.001 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0024 | 0.0004 | 0.0011 | 0.0006 | 0.0108 | 0.0012 | 0.003 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0028 | 0.0008 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0011 | 0.0005 | 0.0004 | 0.0003 | 0.0015 | 0.0003 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 |
Peroxisomes | 0.0137 | 0.0362 | 0.0127 | 0.004 | 0.009 | 0.0045 | 0.0305 | 0.0196 | 0.0097 | 0.0422 | 0.0194 | 0.0135 | 0.009 | 0.02 | 0.0177 | 0.0079 | 0.0106 | 0.005 |
Punctate Nuclear | 0.0012 | 0.0001 | 0 | 0 | 0.0049 | 0 | 0.0056 | 0.0001 | 0.0013 | 0.0036 | 0.0086 | 0.0003 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0022 | 0.0086 | 0.0021 | 0.0027 | 0.0012 | 0.0012 | 0.0045 | 0.0201 | 0.0046 | 0.0016 | 0.008 | 0.0024 | 0.0025 | 0.0146 | 0.0022 | 0.0005 | 0.005 | 0.0011 |
Vacuole Periphery | 0.0035 | 0.0145 | 0.0035 | 0.0053 | 0.0019 | 0.0043 | 0.0039 | 0.0139 | 0.0042 | 0.0022 | 0.0068 | 0.0044 | 0.0044 | 0.0114 | 0.0036 | 0.001 | 0.0093 | 0.0035 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.598 | 26.0195 | 28.0838 | 43.5036 | 18.869 | 63.4701 | 50.2249 | 68.604 | 65.3019 | 43.3286 |
Translational Efficiency | 1.2602 | 2.4551 | 2.499 | 2.0294 | 3.0691 | 1.9299 | 1.8865 | 1.9224 | 2.0416 | 1.8458 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1834 | 1886 | 733 | 232 | 1649 | 1786 | 1829 | 768 | 3483 | 3672 | 2562 | 1000 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 782.89 | 906.04 | 1142.09 | 1331.68 | 847.10 | 906.84 | 1019.74 | 1141.32 | 813.29 | 906.43 | 1054.74 | 1185.48 |
Standard Deviation | 136.82 | 139.82 | 145.16 | 227.03 | 124.19 | 117.78 | 130.82 | 221.60 | 134.86 | 129.57 | 145.96 | 236.91 |
Intensity Change Log 2 | — | 0.210765 | 0.544795 | 0.766366 | — | 0.098316 | 0.267597 | 0.430099 | — | 0.153420 | 0.407390 | 0.601399 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000853 | 0.001637 | 0.006114 | 0.014261 | 0.000458 | 0.000640 | 0.002287 | 0.013269 | 0.000666 | 0.001152 | 0.003381 | 0.013499 |
Bud Neck | 0.003215 | 0.000517 | 0.005316 | 0.002861 | 0.000474 | 0.000857 | 0.003879 | 0.002730 | 0.001917 | 0.000682 | 0.004290 | 0.002760 |
Bud Site | 0.009930 | 0.002047 | 0.020610 | 0.011050 | 0.001385 | 0.001960 | 0.020452 | 0.011708 | 0.005885 | 0.002005 | 0.020497 | 0.011556 |
Cell Periphery | 0.000499 | 0.000214 | 0.000415 | 0.000449 | 0.000387 | 0.000273 | 0.000236 | 0.001373 | 0.000446 | 0.000243 | 0.000287 | 0.001158 |
Cytoplasm | 0.004238 | 0.000633 | 0.003762 | 0.001742 | 0.000735 | 0.000485 | 0.003577 | 0.001191 | 0.002580 | 0.000561 | 0.003630 | 0.001319 |
Cytoplasmic Foci | 0.027605 | 0.020338 | 0.085890 | 0.021377 | 0.004343 | 0.016609 | 0.029833 | 0.015224 | 0.016592 | 0.018524 | 0.045871 | 0.016651 |
Eisosomes | 0.000061 | 0.000049 | 0.000074 | 0.000428 | 0.000021 | 0.000035 | 0.000053 | 0.000930 | 0.000042 | 0.000042 | 0.000059 | 0.000814 |
Endoplasmic Reticulum | 0.000529 | 0.000144 | 0.001453 | 0.001982 | 0.000163 | 0.000201 | 0.000382 | 0.000531 | 0.000356 | 0.000172 | 0.000688 | 0.000867 |
Endosome | 0.060048 | 0.031089 | 0.135852 | 0.024569 | 0.010343 | 0.050159 | 0.053750 | 0.022059 | 0.036516 | 0.040364 | 0.077240 | 0.022642 |
Golgi | 0.060044 | 0.032348 | 0.142922* | 0.081033 | 0.007681 | 0.033085 | 0.041149 | 0.041090 | 0.035253 | 0.032707 | 0.070267 | 0.050357 |
Lipid Particles | 0.005431 | 0.001793 | 0.002403 | 0.006599 | 0.001463 | 0.003994 | 0.001045 | 0.001332 | 0.003553 | 0.002864 | 0.001434 | 0.002554 |
Mitochondria | 0.768321* | 0.872061* | 0.367767* | 0.750555* | 0.955136* | 0.858243* | 0.709564* | 0.845121* | 0.856767* | 0.865340* | 0.611774* | 0.823182* |
Mitotic Spindle | 0.004656 | 0.003713 | 0.076200 | 0.025707 | 0.001540 | 0.002053 | 0.053925 | 0.007756 | 0.003181 | 0.002906 | 0.060298 | 0.011921 |
None | 0.000708 | 0.000148 | 0.000324 | 0.001282 | 0.000383 | 0.000196 | 0.000313 | 0.000366 | 0.000554 | 0.000171 | 0.000316 | 0.000579 |
Nuclear Periphery | 0.000115 | 0.000072 | 0.001754 | 0.001314 | 0.000046 | 0.000095 | 0.000422 | 0.000080 | 0.000082 | 0.000083 | 0.000803 | 0.000366 |
Nuclear Periphery Foci | 0.000293 | 0.000110 | 0.002022 | 0.000894 | 0.000073 | 0.000144 | 0.000426 | 0.000099 | 0.000189 | 0.000127 | 0.000883 | 0.000283 |
Nucleolus | 0.000770 | 0.000070 | 0.000643 | 0.000115 | 0.000131 | 0.000103 | 0.000243 | 0.000057 | 0.000467 | 0.000086 | 0.000358 | 0.000071 |
Nucleus | 0.001821 | 0.000052 | 0.001247 | 0.000115 | 0.000124 | 0.000089 | 0.000783 | 0.000079 | 0.001017 | 0.000070 | 0.000916 | 0.000087 |
Peroxisomes | 0.023143 | 0.023941 | 0.025402 | 0.012192 | 0.007015 | 0.012289 | 0.032830 | 0.026064 | 0.015508 | 0.018274 | 0.030705 | 0.022846 |
Vacuole | 0.011449 | 0.004115 | 0.062127 | 0.018236 | 0.003071 | 0.009057 | 0.023676 | 0.003900 | 0.007482 | 0.006519 | 0.034677 | 0.007226 |
Vacuole Periphery | 0.016271 | 0.004910 | 0.057703 | 0.023238 | 0.005027 | 0.009431 | 0.021175 | 0.005040 | 0.010948 | 0.007109 | 0.031626 | 0.009262 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.54 | -4.28 | -3.31 | -3.11 | -1.92 | -0.89 | -5.66 | -5.79 | -5.71 | -4.93 | -2.60 | -6.42 | -6.61 | -6.35 | -5.11 |
Bud Neck | 5.59 | -2.49 | 0.47 | -4.10 | 2.73 | -1.51 | -7.34 | -3.62 | -3.13 | 1.62 | 4.38 | -5.32 | -1.57 | -4.39 | 2.64 |
Bud Site | 6.39 | -4.33 | -0.26 | -1.98 | 1.91 | -1.18 | -10.96 | -4.99 | -4.74 | 3.37 | 5.63 | -9.69 | -2.84 | -5.04 | 3.91 |
Cell Periphery | 3.44 | 0.51 | 0.37 | -1.82 | -0.17 | 0.79 | 1.23 | -2.57 | -2.96 | -3.13 | 2.51 | 1.92 | -2.46 | -3.23 | -3.07 |
Cytoplasm | 4.45 | 0.20 | 1.27 | -1.42 | 1.09 | 0.78 | -6.16 | -1.26 | -3.80 | 6.48 | 4.44 | -2.05 | 2.07 | -2.85 | 4.93 |
Cytoplasmic Foci | 3.51 | -12.56 | 1.26 | -0.46 | 10.74 | -9.01 | -14.66 | -5.79 | 0.63 | 6.02 | -1.49 | -14.76 | -0.14 | 0.86 | 11.88 |
Eisosomes | 2.16 | -1.98 | -2.20 | -2.28 | -2.12 | -6.60 | -13.27 | -1.43 | -1.40 | -1.38 | -0.00 | -4.96 | -1.57 | -1.57 | -1.54 |
Endoplasmic Reticulum | 5.65 | -4.92 | -1.46 | -1.85 | -0.50 | -0.56 | -5.01 | -4.16 | -3.28 | -1.47 | 3.85 | -5.06 | -2.11 | -2.89 | -0.64 |
Endosome | 8.78 | -11.48 | 7.16 | 1.45 | 15.31 | -13.48 | -16.33 | -4.41 | 7.81 | 9.45 | -1.68 | -13.61 | 5.30 | 6.77 | 16.79 |
Golgi | 8.28 | -11.16 | -1.83 | -4.34 | 4.76 | -11.70 | -14.76 | -8.21 | -1.81 | -0.00 | 1.22 | -11.37 | -3.49 | -4.16 | 4.13 |
Lipid Particles | 3.59 | 2.95 | -0.48 | -2.14 | -1.85 | -3.12 | 1.07 | 0.29 | 3.22 | -0.67 | 1.05 | 3.77 | 1.28 | 0.44 | -1.80 |
Mitochondria | -11.48 | 27.11 | 0.88 | 5.55 | -15.13 | 14.28 | 28.08 | 10.76 | 1.18 | -10.94 | -1.47 | 28.32 | 3.40 | 4.34 | -18.12 |
Mitotic Spindle | 1.22 | -11.86 | -3.32 | -3.46 | 5.82 | -0.99 | -16.22 | -4.18 | -3.92 | 13.17 | 0.59 | -19.80 | -4.67 | -4.80 | 14.21 |
None | 3.75 | 2.58 | -1.27 | -2.75 | -2.32 | 0.98 | 0.39 | 0.11 | -3.80 | -1.88 | 3.19 | 2.03 | -0.09 | -4.08 | -2.66 |
Nuclear Periphery | 2.15 | -5.08 | -4.86 | -5.03 | 1.08 | -3.82 | -3.51 | -2.18 | 0.78 | 3.16 | -0.09 | -5.98 | -4.67 | -4.62 | 3.24 |
Nuclear Periphery Foci | 3.53 | -6.80 | -2.97 | -3.95 | 3.60 | -2.45 | -5.82 | -0.95 | 1.76 | 5.53 | 2.08 | -7.96 | -1.69 | -3.07 | 6.28 |
Nucleolus | 2.08 | 0.19 | 1.96 | -0.90 | 2.63 | 0.30 | -1.15 | 0.81 | 1.36 | 4.04 | 2.09 | 0.50 | 2.19 | 0.55 | 4.22 |
Nucleus | 2.69 | 0.39 | 2.46 | -1.24 | 2.59 | 0.53 | -1.57 | 0.63 | 0.26 | 1.67 | 2.72 | -0.14 | 2.62 | -0.97 | 2.53 |
Peroxisomes | -0.27 | -0.65 | 2.55 | 2.72 | 2.76 | -2.86 | -9.59 | -4.98 | -3.56 | 1.59 | -1.59 | -6.77 | -2.31 | -1.44 | 2.29 |
Vacuole | 7.12 | -13.71 | -1.50 | -2.93 | 6.86 | -5.75 | -12.64 | -0.99 | 5.25 | 12.41 | 1.31 | -16.82 | 0.00 | -0.70 | 13.79 |
Vacuole Periphery | 10.48 | -10.50 | -1.66 | -4.47 | 6.17 | -4.90 | -10.17 | -0.02 | 4.61 | 9.97 | 5.33 | -12.47 | 1.35 | -1.82 | 11.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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