Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.99 0.96 0.93 0.88 0.88 0.79 0.77 0.63 0.65 1.0 0.99 0.99 0.97 0.96 0.96 0.93 0.91 0.91 0.96 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.07 0.12 0.16 0.35 0.25 0.43 0.47 0.66 0.56 0 0 0 0 0 0 0 0.05 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 0 1 0 2 2
Bud 1 0 3 3 1 3 20 6 10 21 23 9 0 0 1 0 0 0 0 0 6 0 6 11
Bud Neck 0 0 0 1 0 0 2 2 0 4 1 0 1 3 0 0 0 0 0 0 1 0 2 4
Bud Site 0 0 0 0 0 1 0 0 0 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 14 5 8 5 5 8 5 5 0 5 5 8 1 8 12 12 0 0 0 0 0 0
Cytoplasm 300 163 437 383 557 518 896 843 424 569 314 345 411 737 647 196 217 234 298 160 402 317 485 520
Endoplasmic Reticulum 2 1 2 0 0 0 0 0 0 0 0 11 0 2 2 14 11 14 0 0 6 1 3 2
Endosome 0 0 2 0 6 5 9 12 3 2 0 2 1 2 0 1 2 1 0 0 3 1 2 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 4 0
Mitochondria 10 9 3 27 67 89 355 244 228 347 332 296 0 0 1 6 2 7 4 8 10 1 4 5
Nucleus 1 1 1 0 0 1 0 1 3 2 0 0 2 2 0 0 4 3 0 0 0 1 2 3
Nuclear Periphery 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 3 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 3 9 5 3 2 5 0 1 0 0 0 1 1 0 2 1 4 5
Vac/Vac Membrane 1 0 3 2 5 1 10 23 4 13 5 10 0 0 2 2 3 5 6 1 0 0 0 1
Unique Cell Count 307 170 444 388 582 556 1021 961 535 736 501 532 412 743 652 202 227 243 321 176 443 330 526 575
Labelled Cell Count 316 175 465 421 645 626 1301 1151 682 969 681 685 420 755 655 227 252 277 321 176 443 330 526 575


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.5 4.6 4.3 4.2 3.6 3.5 3.6 3.5 3.0 2.9 3.0 5.7 5.9 5.7 7.2 7.4 6.5 6.0 6.3 6.3
Std Deviation (1e-4) 0.5 0.9 1.1 1.4 1.4 0.8 1.4 1.4 1.5 1.1 0.8 0.6 0.8 0.9 1.1 1.2 1.2 1.1 1.1 1.4 1.5
Intensity Change (Log2) – – – -0.11 -0.11 -0.35 -0.4 -0.35 -0.39 -0.61 -0.68 -0.63 0.31 0.35 0.3 0.65 0.69 0.49 0.39 0.46 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3698 3.8732 3.613 3.0779 2.8607 3.4249 -1.339 -0.5548 -1.0793 -1.2858 -0.08 -1.033 -1.2895 -0.5638 -0.9 -0.6771 -0.717 -1.043
Actin 0.0406 0.0004 0.0153 0.0015 0.0015 0.0055 0.0511 0.0002 0.0238 0.0001 0.0176 0.018 0.0382 0.0007 0.0181 0.0098 0.003 0.0014
Bud 0.0013 0.0026 0.0067 0.0032 0.0022 0.0019 0.0036 0.0001 0.0029 0.0004 0.0179 0.0012 0.0018 0.0058 0.0101 0.0012 0.0236 0.0006
Bud Neck 0.0072 0.0003 0.003 0.0004 0.0003 0.0008 0.0043 0.0001 0.0005 0.0001 0.017 0.001 0.0013 0.0001 0.0011 0.0008 0.0002 0.0002
Bud Periphery 0.0039 0.0032 0.0039 0.0139 0.0103 0.0013 0.0075 0.0001 0.0059 0.0003 0.0563 0.003 0.0013 0.0013 0.0034 0.0017 0.0091 0.0037
Bud Site 0.0101 0.0224 0.0097 0.0012 0.0002 0.0002 0.0151 0.0006 0.0068 0.0001 0.0426 0.0001 0.0044 0.0072 0.0074 0.0021 0.0004 0.0001
Cell Periphery 0.0004 0.0004 0.0004 0.0002 0.0003 0.0002 0.0011 0.0002 0.0004 0 0.0027 0.0001 0.0005 0.0003 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.0893 0.2845 0.2048 0.1755 0.1355 0.219 0.2402 0.4393 0.4246 0.4578 0.3028 0.504 0.1337 0.3403 0.2743 0.2504 0.3884 0.273
Cytoplasmic Foci 0.0115 0.0055 0.0091 0.0094 0.017 0.0072 0.0382 0.008 0.0238 0.0116 0.0383 0.0119 0.019 0.0035 0.0095 0.007 0.0167 0.0189
Eisosomes 0.0009 0.0001 0.0004 0.0001 0.0012 0.0002 0.0004 0.0001 0.0002 0 0.0003 0.0001 0.0007 0.0001 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.0031 0.0013 0.0013 0.0018 0.0015 0.0017 0.006 0.0038 0.004 0.0018 0.0088 0.0026 0.0024 0.001 0.0015 0.0013 0.0031 0.0015
Endosome 0.0202 0.0008 0.0027 0.0145 0.0011 0.0024 0.0191 0.0035 0.0176 0.0127 0.0671 0.0192 0.0132 0.001 0.002 0.0095 0.0352 0.0048
Golgi 0.0056 0.0001 0.0013 0.0012 0.0003 0.0012 0.0078 0.0002 0.0023 0.0002 0.025 0.0013 0.0056 0.0001 0.0012 0.0104 0.0032 0.0003
Lipid Particles 0.0069 0.0001 0.0011 0.0047 0.0014 0.0017 0.0103 0.0012 0.0031 0 0.0914 0.0004 0.0068 0.0001 0.0011 0.0011 0.0035 0.0006
Mitochondria 0.0048 0.0003 0.0104 0.0032 0.001 0.0017 0.0021 0.0002 0.011 0.0002 0.009 0.0011 0.0045 0.0002 0.0014 0.0048 0.0033 0.0007
None 0.7613 0.675 0.7173 0.7576 0.8198 0.7456 0.5584 0.5383 0.4563 0.5085 0.248 0.4308 0.7335 0.6361 0.6587 0.6957 0.4847 0.6887
Nuclear Periphery 0.0059 0.0003 0.0009 0.0012 0.0008 0.0022 0.005 0.0007 0.0018 0.0007 0.0068 0.0008 0.0065 0.0003 0.0008 0.0004 0.0153 0.0021
Nucleolus 0.0014 0.0002 0.0008 0.0017 0.0012 0.0003 0.0012 0.0001 0.001 0 0.0027 0.0001 0.0015 0.0001 0.0006 0.0001 0.0005 0.0001
Nucleus 0.0051 0.0006 0.0047 0.002 0.0009 0.0014 0.0031 0.0007 0.0015 0.0009 0.0043 0.0007 0.007 0.0006 0.0023 0.0004 0.0021 0.0008
Peroxisomes 0.0058 0.0002 0.0027 0.0028 0.0008 0.0035 0.0052 0.0001 0.0047 0 0.0114 0.0002 0.0091 0 0.0019 0.0015 0.0007 0.0001
Punctate Nuclear 0.0117 0.0008 0.0018 0.0026 0.0017 0.0012 0.0158 0.0004 0.002 0.0004 0.0127 0.0005 0.0061 0.0002 0.0026 0.0003 0.0044 0.0015
Vacuole 0.002 0.0007 0.0015 0.001 0.0008 0.0007 0.0038 0.0019 0.0049 0.0034 0.0143 0.0022 0.0019 0.0008 0.0012 0.0011 0.0017 0.0006
Vacuole Periphery 0.001 0.0001 0.0002 0.0004 0.0002 0.0003 0.0008 0.0002 0.0009 0.0006 0.0031 0.0006 0.001 0.0001 0.0002 0.0003 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.958 18.7881 11.618 15.1844 21.005 9.6276 10.2899 12.0201 11.5728 19.6007
Translational Efficiency 0.9698 1.0772 1.6276 1.0717 0.884 1.9141 1.2111 0.8541 0.7481 0.8208

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1412 898 2008 377 1447 1714 297 710 2859 2612 2305 1087

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 660.35 721.79 834.88 920.79 671.17 759.88 953.84 896.40 665.83 746.78 850.21 904.86
Standard Deviation 87.05 146.12 95.94 115.96 84.23 288.77 110.16 115.33 85.81 249.77 105.69 116.13
Intensity Change Log 2 — 0.128348 0.338338 0.479641 — 0.179093 0.507069 0.417464 — 0.154150 0.425853 0.448635

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000172 0.000594 0.000227 0.000267 0.000215 0.000512 0.000350 0.006479 0.000194 0.000540 0.000243 0.004325
Bud Neck 0.016074 0.033776 0.004520 0.002865 0.011289 0.029643 0.007411 0.003349 0.013652 0.031064 0.004892 0.003181
Bud Site 0.007754 0.026390 0.014361 0.010969 0.007995 0.017336 0.011361 0.023827 0.007876 0.020449 0.013975 0.019367
Cell Periphery 0.000171 0.000163 0.000102 0.000134 0.000155 0.000134 0.000185 0.000277 0.000163 0.000144 0.000113 0.000227
Cytoplasm 0.532615* 0.397182* 0.616708* 0.554325* 0.539006* 0.504179* 0.387905* 0.544785* 0.535850* 0.467394* 0.587226* 0.548093*
Cytoplasmic Foci 0.256573* 0.246210* 0.014931 0.043300 0.233852* 0.195308* 0.037176 0.048894 0.245073* 0.212808* 0.017797 0.046954
Eisosomes 0.000196 0.000162 0.000025 0.000058 0.000179 0.000127 0.000108 0.000063 0.000187 0.000139 0.000036 0.000061
Endoplasmic Reticulum 0.001349 0.000903 0.001423 0.002490 0.001220 0.000506 0.002802 0.000965 0.001284 0.000643 0.001601 0.001494
Endosome 0.016454 0.050006 0.001964 0.009870 0.013866 0.025473 0.004999 0.017535 0.015144 0.033908 0.002355 0.014876
Golgi 0.003421 0.010204 0.000389 0.002523 0.004650 0.004187 0.000104 0.003782 0.004043 0.006255 0.000353 0.003345
Lipid Particles 0.006865 0.009392 0.000790 0.002742 0.008148 0.003941 0.002432 0.001532 0.007514 0.005815 0.001002 0.001952
Mitochondria 0.003620 0.009170 0.000755 0.000145 0.003981 0.005908 0.000260 0.013876 0.003803 0.007029 0.000691 0.009113
Mitotic Spindle 0.000457 0.005267 0.007495 0.006336 0.005670 0.008234 0.001533 0.028574 0.003095 0.007214 0.006727 0.020861
None 0.001938 0.005803 0.002842 0.003284 0.002011 0.001966 0.003834 0.002336 0.001975 0.003285 0.002970 0.002665
Nuclear Periphery 0.000266 0.000502 0.001085 0.000994 0.000378 0.000288 0.001287 0.000495 0.000323 0.000362 0.001111 0.000668
Nuclear Periphery Foci 0.000377 0.000655 0.000953 0.004467 0.000576 0.000215 0.001767 0.001877 0.000478 0.000366 0.001058 0.002775
Nucleolus 0.000958 0.003616 0.000171 0.000194 0.001281 0.000969 0.000557 0.000345 0.001121 0.001879 0.000221 0.000293
Nucleus 0.073597 0.063474 0.254334* 0.111737 0.097470 0.069253 0.324524* 0.063521 0.085679 0.067267 0.263378* 0.080244
Peroxisomes 0.004237 0.015872 0.000437 0.000296 0.003185 0.007149 0.000801 0.002228 0.003705 0.010148 0.000484 0.001558
Vacuole 0.071110 0.117098 0.076275 0.242743* 0.063729 0.122558 0.210298* 0.231843* 0.067374 0.120681 0.093544 0.235624*
Vacuole Periphery 0.001796 0.003559 0.000211 0.000263 0.001144 0.002113 0.000305 0.003417 0.001466 0.002610 0.000223 0.002323

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.86 -1.04 -2.33 3.99 -0.47 -2.30 -2.73 -1.48 -1.24 -1.28 -3.95 -1.25 -1.52 -1.10 -1.45
Bud Neck -5.70 8.05 9.22 10.99 2.12 -9.50 3.69 9.59 15.54 1.90 -10.38 9.54 13.28 18.99 3.51
Bud Site -8.01 -2.11 0.29 6.47 1.64 -4.89 -0.10 -2.14 1.71 -1.60 -8.87 -1.95 -1.90 4.87 -0.15
Cell Periphery 0.26 2.24 2.25 4.54 0.13 1.62 -0.90 0.62 0.18 0.88 1.04 3.28 1.37 0.90 -0.31
Cytoplasm 11.37 -1.16 7.78 -0.44 8.58 3.69 16.50 7.46 4.71 -9.19 9.23 3.63 10.51 3.56 7.35
Cytoplasmic Foci 1.38 44.27 38.64 29.77 -6.37 5.61 36.84 35.86 31.97 -3.89 6.16 59.82 52.46 43.17 -9.54
Eisosomes 1.53 9.29 7.75 7.61 -7.62 3.55 4.60 9.10 9.41 7.96 3.84 13.10 11.72 12.06 -4.38
Endoplasmic Reticulum 1.23 -1.96 -2.88 -6.58 -2.01 4.26 -8.08 0.65 -5.35 8.52 3.35 -5.71 -1.41 -8.36 6.37
Endosome -10.36 12.41 6.51 13.72 -1.70 -7.28 10.07 4.53 9.89 -1.50 -12.03 17.56 7.45 16.40 -3.46
Golgi -5.09 8.93 3.74 6.62 -0.74 0.58 5.70 4.00 5.64 -3.13 -3.12 8.45 5.63 8.64 -2.36
Lipid Particles -2.18 12.33 8.22 6.50 -2.51 5.71 7.48 9.83 9.20 5.71 2.81 14.90 13.46 9.78 -1.72
Mitochondria -3.33 5.01 5.92 5.90 4.01 -1.65 4.59 -0.14 0.83 -2.22 -3.50 6.35 0.77 2.98 -1.94
Mitotic Spindle -3.06 -5.58 -3.11 -1.52 -1.17 -1.14 2.08 -2.60 -1.88 -4.12 -2.89 -2.39 -4.20 -2.38 -2.89
None -2.73 -2.13 -2.57 1.47 -1.59 0.11 -2.98 -0.42 -0.36 2.69 -2.03 -3.77 -2.02 0.90 0.84
Nuclear Periphery -2.72 -21.60 -8.88 -6.53 -0.44 1.36 -13.40 -7.17 -10.15 9.86 -0.98 -23.24 -11.91 -11.00 6.29
Nuclear Periphery Foci -2.29 -5.52 -8.34 -7.68 -6.26 4.41 -6.93 -2.81 -3.48 1.05 1.38 -8.15 -5.36 -5.69 -3.03
Nucleolus -2.38 2.44 2.27 3.77 -1.23 0.89 2.25 3.10 5.08 2.40 -1.40 3.79 3.75 5.14 -0.17
Nucleus 1.71 -35.61 -7.34 -7.78 16.33 6.98 -14.60 1.17 -3.99 14.66 5.90 -37.46 -4.39 -8.06 28.46
Peroxisomes -8.17 10.45 10.94 11.86 1.07 -5.98 8.16 6.38 9.91 -1.23 -9.62 13.89 11.18 15.32 -1.56
Vacuole -8.38 -10.09 -19.89 -16.10 -16.61 -13.51 -15.23 -27.70 -21.63 -2.09 -15.52 -16.94 -33.97 -26.97 -23.25
Vacuole Periphery -2.99 7.77 7.88 6.34 0.57 -3.17 8.76 0.16 2.03 -2.13 -3.91 10.94 2.40 4.84 -2.13
Standard name
Human Ortholog
Description S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis
Localization
Cell Percentages cytoplasm (61%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Efm2

Efm2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Efm2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available