Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.97 0.98 0.88 0.77 0.73 – 0.8 0.68 0.7 0.99 0.99 1.0 0.99 0.99 0.99 0.93 0.96 0.96 0.89 0.92 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.11 0.14 0.32 0.47 0.53 – 0.45 0.56 0.55 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Bud 0 0 0 0 1 8 11 7 – 3 8 14 0 0 0 0 1 0 2 0 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 1 – 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 1 – 1 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 2 0 2 – 3 1 4 4 3 8 30 27 9 0 0 0 0 0 0
Cytoplasm 128 90 151 205 292 309 321 442 – 397 218 283 293 454 501 310 351 227 125 97 149 214 250 294
Endoplasmic Reticulum 1 1 2 1 0 1 1 0 – 0 0 1 2 0 0 15 11 13 0 0 0 3 4 4
Endosome 0 0 0 0 0 0 0 1 – 0 0 1 0 0 0 0 0 0 0 1 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 2 1 19 24 41 112 195 324 – 224 180 222 0 0 0 3 1 0 1 0 1 5 2 4
Nucleus 0 0 1 0 0 2 3 4 – 2 0 0 3 2 0 0 1 0 0 0 0 1 0 0
Nuclear Periphery 0 0 1 0 0 0 3 1 – 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 – 4 3 3 0 1 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 0 0 1 0 0 4 4 – 4 4 0 0 1 2 1 1 1 0 0 0 11 5 8
Unique Cell Count 130 93 153 211 299 350 415 608 498 321 407 297 457 501 313 355 229 136 102 155 242 271 327
Labelled Cell Count 131 93 175 232 335 434 540 789 639 416 539 302 461 511 360 393 251 136 102 155 242 271 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.6 4.4 4.8 4.3 4.0 3.3 3.3 – 3.0 2.9 3.0 6.6 6.8 6.8 6.6 6.4 6.7 7.1 7.4 7.6
Std Deviation (1e-4) 0.8 1.1 0.5 1.4 0.8 1.3 1.1 0.9 – 1.0 1.1 1.2 0.8 1.3 1.1 1.0 1.0 1.0 1.2 1.7 1.4
Intensity Change (Log2) – – – 0.14 -0.0 -0.11 -0.39 -0.42 – -0.52 -0.58 -0.54 0.6 0.65 0.64 0.6 0.57 0.63 0.7 0.77 0.8


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 3.6 3.1 0
Cytoplasm -1.0 -0.7 -3.8 -6.0 -6.9 – -5.6 -7.5 -7.4 0 0.8 2.6 0.3 0.2 0.4
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 1.9 0 2.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -6.2 0 0 -5.5 -6.5 -5.5
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1518 1.7635 1.6398 1.1239 1.0159 1.7129 1.9147 3.2981 2.5352 2.6594 2.5111 2.7857 -0.132 0.1951 -0.2724 -0.1354 -0.2529 -0.2334
Actin 0.0066 0.0016 0.0022 0.0001 0.011 0.0014 0.0034 0.0003 0.0017 0.0007 0.0004 0.0008 0.0194 0.0001 0.0001 0.0002 0.007 0.0001
Bud 0.002 0.0012 0.0017 0.0003 0.0008 0.0005 0.0002 0.0003 0.0004 0.0003 0.0002 0.0001 0.0006 0.0001 0.0001 0.0002 0.0003 0
Bud Neck 0.0019 0.0004 0.0014 0.0002 0.0004 0.001 0.0005 0.0001 0.0006 0.0002 0.0003 0.0003 0.003 0 0 0.0001 0.0003 0.0003
Bud Periphery 0.0016 0.0012 0.0013 0.0001 0.0014 0.0003 0.0002 0.0002 0.0004 0.0005 0.0002 0.0001 0.0007 0 0 0.0002 0.0002 0
Bud Site 0.0054 0.0045 0.0075 0.0001 0.0009 0.0001 0.0005 0.002 0.002 0.0002 0.0002 0 0.0158 0.0003 0.0002 0.0001 0.0006 0
Cell Periphery 0.0003 0.0002 0.0002 0 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0.0001 0.0001 0
Cytoplasm 0.4795 0.7172 0.5806 0.6992 0.5548 0.6814 0.5418 0.646 0.5813 0.5757 0.6169 0.6447 0.3674 0.6691 0.7878 0.64 0.5292 0.8913
Cytoplasmic Foci 0.023 0.0149 0.015 0.0164 0.013 0.015 0.0164 0.0084 0.0143 0.01 0.0349 0.0071 0.0311 0.0071 0.0047 0.0093 0.0309 0.0033
Eisosomes 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0005 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0038 0.0017 0.0024 0.0018 0.0022 0.0029 0.0035 0.0016 0.0039 0.0015 0.0018 0.0009 0.0135 0.0041 0.0022 0.0029 0.0017 0.0015
Endosome 0.0127 0.006 0.0104 0.0161 0.0064 0.0078 0.0092 0.0042 0.0089 0.0064 0.011 0.0044 0.0329 0.0033 0.0033 0.0156 0.1042 0.0016
Golgi 0.0014 0.0006 0.001 0.0003 0.0007 0.0008 0.0014 0.0004 0.0008 0.0003 0.0005 0.0007 0.0192 0.0002 0.0002 0.0005 0.0369 0.0001
Lipid Particles 0.0012 0.0003 0.0003 0.0001 0.0003 0.0001 0.002 0.0001 0.0004 0.0003 0.0002 0.0024 0.0596 0.0003 0.0001 0.0002 0.0128 0
Mitochondria 0.0007 0.0004 0.0012 0.0004 0.0007 0.0017 0.0005 0.0003 0.0006 0.0008 0.0003 0.0004 0.0077 0.0002 0.0001 0.0002 0.0549 0.0001
None 0.447 0.2436 0.367 0.2586 0.4023 0.2815 0.4105 0.3316 0.3739 0.3977 0.3299 0.3347 0.3693 0.3105 0.198 0.3169 0.1195 0.1004
Nuclear Periphery 0.0011 0.0006 0.0009 0.0009 0.0007 0.0005 0.0015 0.0003 0.002 0.0005 0.0003 0.0002 0.0246 0.0009 0.0006 0.0012 0.0005 0.0002
Nucleolus 0.0006 0.0002 0.0003 0 0.0001 0.0001 0.0002 0.0001 0.0003 0.0001 0.0001 0 0.0079 0 0 0.0001 0.0002 0
Nucleus 0.0021 0.0017 0.0018 0.0015 0.0012 0.0013 0.0008 0.0007 0.0025 0.0008 0.0005 0.0004 0.0044 0.0006 0.0004 0.0006 0.0005 0.0003
Peroxisomes 0.0025 0.0004 0.0005 0.0001 0.0006 0.0003 0.0017 0.0001 0.0008 0.0002 0.0006 0.0016 0.0087 0 0 0.0001 0.0933 0
Punctate Nuclear 0.0032 0.0009 0.001 0.0004 0.0006 0.0018 0.003 0.0003 0.0018 0.0003 0.0004 0.0002 0.0061 0.0002 0.0001 0.0005 0.0004 0
Vacuole 0.0029 0.002 0.0024 0.0027 0.0013 0.0011 0.0021 0.0026 0.0027 0.0027 0.0011 0.0008 0.0043 0.0024 0.0017 0.0093 0.0046 0.0005
Vacuole Periphery 0.0004 0.0003 0.0005 0.0006 0.0003 0.0002 0.0003 0.0002 0.0004 0.0005 0.0002 0.0002 0.0025 0.0002 0.0002 0.0019 0.0019 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7936 12.9876 8.6229 11.4382 11.4013 12.2278 14.1685 10.729 15.0968 15.6515
Translational Efficiency 1.5009 1.1171 1.2391 1.2753 1.1935 1.2139 1.3136 1.1573 1.1103 1.1081

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1975 1826 107 162 576 1757 215 1568 2551 3583 322 1730

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 737.43 860.25 1178.37 1155.60 812.15 814.15 1115.13 1088.41 754.30 837.64 1136.14 1094.70
Standard Deviation 92.37 126.88 133.96 157.51 105.71 106.14 126.30 153.07 100.53 119.42 132.30 154.73
Intensity Change Log 2 — 0.222250 0.676215 0.648064 — 0.003548 0.457394 0.422404 — 0.111767 0.565674 0.534202

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000226 0.000530 0.000330 0.000242 0.000383 0.000744 0.000674 0.000129 0.000303 0.000673 0.000642
Bud Neck 0.008222 0.007457 0.001175 0.001824 0.007180 0.014586 0.001209 0.001326 0.007987 0.010953 0.001198 0.001373
Bud Site 0.007049 0.017847 0.010101 0.011379 0.008179 0.024121 0.015933 0.016951 0.007304 0.020924 0.013995 0.016430
Cell Periphery 0.000085 0.000106 0.000181 0.000076 0.000182 0.000095 0.000255 0.000058 0.000107 0.000101 0.000231 0.000060
Cytoplasm 0.624022* 0.639343* 0.777882* 0.485055* 0.558027* 0.535972* 0.762230* 0.819389* 0.609121* 0.588653* 0.767431* 0.788081*
Cytoplasmic Foci 0.201482* 0.192723* 0.065580 0.066229 0.275782* 0.232220* 0.054079 0.052115 0.218259* 0.212091* 0.057901 0.053436
Eisosomes 0.000070 0.000054 0.000019 0.000033 0.000130 0.000081 0.000023 0.000010 0.000084 0.000067 0.000022 0.000012
Endoplasmic Reticulum 0.000556 0.000867 0.001188 0.000417 0.001162 0.000906 0.001144 0.000127 0.000693 0.000886 0.001159 0.000154
Endosome 0.015117 0.016137 0.004216 0.005466 0.024456 0.029296 0.004268 0.003054 0.017225 0.022590 0.004251 0.003280
Golgi 0.004086 0.006972 0.000444 0.000160 0.003401 0.009661 0.000543 0.001959 0.003931 0.008290 0.000510 0.001791
Lipid Particles 0.003362 0.002054 0.001016 0.000826 0.006544 0.002500 0.000754 0.000235 0.004080 0.002273 0.000841 0.000290
Mitochondria 0.002693 0.002342 0.000045 0.000034 0.002017 0.005041 0.000294 0.000735 0.002541 0.003665 0.000211 0.000670
Mitotic Spindle 0.000553 0.003794 0.002343 0.005074 0.001307 0.006858 0.000556 0.006899 0.000723 0.005296 0.001150 0.006728
None 0.002870 0.001324 0.000755 0.005226 0.001078 0.001108 0.001289 0.001200 0.002466 0.001218 0.001111 0.001577
Nuclear Periphery 0.000161 0.000095 0.000214 0.000193 0.000170 0.000166 0.000169 0.000046 0.000163 0.000130 0.000184 0.000059
Nuclear Periphery Foci 0.000334 0.000768 0.012497 0.003073 0.001132 0.000227 0.010115 0.000922 0.000514 0.000503 0.010907 0.001124
Nucleolus 0.000530 0.000449 0.000077 0.000120 0.000274 0.000580 0.000081 0.000082 0.000472 0.000513 0.000080 0.000085
Nucleus 0.045523 0.026809 0.041939 0.029179 0.025219 0.019215 0.029772 0.013020 0.040938 0.023085 0.033815 0.014533
Peroxisomes 0.001595 0.002502 0.000235 0.000553 0.001755 0.004747 0.000376 0.001491 0.001631 0.003603 0.000329 0.001404
Vacuole 0.079830 0.075750 0.079347 0.384591* 0.080294 0.108482 0.115960 0.079375 0.079935 0.091801 0.103794 0.107956
Vacuole Periphery 0.001764 0.002381 0.000217 0.000163 0.001469 0.003757 0.000206 0.000330 0.001697 0.003056 0.000209 0.000314

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.62 -4.74 -4.22 -0.96 3.01 -1.26 -1.16 -0.74 -0.16 0.38 -3.40 -2.50 -1.31 -0.48 0.64
Bud Neck 1.03 10.99 8.16 8.06 -1.88 -5.44 5.47 5.63 15.06 -0.22 -4.20 12.10 12.88 18.94 -0.22
Bud Site -8.80 -2.48 -2.70 4.43 0.37 -8.55 -1.47 -3.04 5.64 0.38 -12.53 -2.16 -4.39 5.78 0.28
Cell Periphery -3.13 -4.85 1.17 3.24 5.05 6.81 -0.05 9.85 5.68 1.66 0.98 -1.50 7.81 6.88 2.51
Cytoplasm -1.81 -4.48 7.20 7.75 8.54 1.85 -9.42 -18.88 -27.76 -2.81 3.12 -8.89 -17.56 -20.71 -1.06
Cytoplasmic Foci 1.55 17.56 23.31 21.22 1.56 4.91 25.75 28.83 35.95 0.85 1.36 31.85 41.73 44.20 2.00
Eisosomes 4.04 9.84 10.20 6.46 -0.87 5.80 12.87 15.59 19.24 4.61 4.50 15.96 24.17 23.63 5.30
Endoplasmic Reticulum -1.82 -2.39 0.37 1.86 2.44 0.76 0.69 5.58 2.70 3.24 -1.09 -1.95 9.09 4.41 4.54
Endosome -0.82 8.54 14.31 12.81 1.54 -2.36 11.83 13.43 22.25 3.30 -5.23 15.57 21.02 25.83 4.10
Golgi -4.25 12.40 13.63 11.28 6.81 -6.37 6.19 3.68 8.85 -1.57 -7.45 13.03 6.42 11.16 -1.51
Lipid Particles 3.24 4.95 5.56 3.55 0.43 2.64 3.74 4.08 12.20 4.87 3.94 6.84 8.36 14.49 5.99
Mitochondria 0.45 4.42 4.42 4.04 0.16 -2.25 1.78 1.53 5.23 -0.98 -1.52 4.57 3.93 6.03 -1.21
Mitotic Spindle -3.46 -1.57 -2.51 -0.69 -1.42 -3.42 0.56 -2.51 1.13 -3.46 -5.29 -1.19 -3.88 0.33 -3.24
None 4.47 6.88 0.50 -0.70 -1.19 -0.08 -0.19 0.06 0.16 0.29 4.45 5.33 3.62 0.20 -0.53
Nuclear Periphery 1.99 -2.54 -3.79 -4.86 -2.04 0.06 -0.68 1.78 1.22 4.68 0.90 -2.15 1.09 -0.11 4.32
Nuclear Periphery Foci -1.85 -6.91 -6.33 -4.88 5.33 7.32 -6.91 0.80 -12.02 7.02 0.05 -10.01 -9.00 -5.17 9.34
Nucleolus 0.48 10.95 6.73 2.19 -2.11 -2.23 6.85 6.54 3.55 -1.32 -0.49 12.02 11.39 4.40 -2.09
Nucleus 9.77 0.03 2.58 -1.42 1.73 3.73 -2.22 4.72 1.24 4.85 12.86 1.53 15.46 3.62 6.37
Peroxisomes -3.72 10.84 9.11 9.14 -2.78 -6.75 6.29 2.33 7.49 -1.77 -7.91 9.54 2.54 7.60 -1.96
Vacuole 1.01 -2.24 -14.41 -14.53 -10.58 -5.63 -5.30 -8.95 -4.18 1.37 -4.44 -5.77 -15.19 -12.68 -1.84
Vacuole Periphery -2.59 13.38 15.17 10.85 2.89 -5.54 5.95 5.69 10.02 -0.77 -6.02 15.06 14.19 13.80 -0.28
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hsm3

Hsm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available